bifidobacterium animalis subspecies lactis  (ATCC)

Journal: International Dental Journal

Article Title: Probiotic Bifidobacteria in Managing Periodontal Disease: A Systematic Review

doi: 10.1016/j.identj.2022.11.018

Figure Lengend Snippet: Characteristics of the included studies on genus Bifidobacterium probiotics used in chronic periodontitis cases in RCTs.

Article Snippet: Argandoña Valdez et al (2021) Brazil , PROB strains: Bifidobacterium longum subspecies longum (ATCC 15,707), Bifidobacterium longum subspecies infantis (ATCC 15,697), and Bifidobacterium animalis subspecies lactis (ATCC 27,673) vs periodontal pathogens: Fusobacterium nucleatum subspecies nucleatum (ATCC 25,585), Porphyromonas gingivalis (33,277), and Streptococcus oralis were grown in brain heart infusion agar , Bacterial strains were incubated for 24 h, 72 h, and 168 h at 37 °C , Checkerboards DNA–DNA hybridisation , The absolute counts of each bacterium in combination were presented in the percentage of bacterial growth.

Techniques:

Journal: International Dental Journal

Article Title: Probiotic Bifidobacteria in Managing Periodontal Disease: A Systematic Review

doi: 10.1016/j.identj.2022.11.018

Figure Lengend Snippet: Characteristics of the included in vitro and animal model studies examining genus Bifidobacterium probiotics.

Article Snippet: Argandoña Valdez et al (2021) Brazil , PROB strains: Bifidobacterium longum subspecies longum (ATCC 15,707), Bifidobacterium longum subspecies infantis (ATCC 15,697), and Bifidobacterium animalis subspecies lactis (ATCC 27,673) vs periodontal pathogens: Fusobacterium nucleatum subspecies nucleatum (ATCC 25,585), Porphyromonas gingivalis (33,277), and Streptococcus oralis were grown in brain heart infusion agar , Bacterial strains were incubated for 24 h, 72 h, and 168 h at 37 °C , Checkerboards DNA–DNA hybridisation , The absolute counts of each bacterium in combination were presented in the percentage of bacterial growth.

Techniques: In Vitro, Animal Model, Incubation, DNA-DNA Hybridization, Inhibition, Cell Culture, Immunohistochemical staining, Micro-CT

Journal: International Dental Journal

Article Title: Probiotic Bifidobacteria in Managing Periodontal Disease: A Systematic Review

doi: 10.1016/j.identj.2022.11.018

Figure Lengend Snippet: Results of the included studies.

Article Snippet: Argandoña Valdez et al (2021) Brazil , PROB strains: Bifidobacterium longum subspecies longum (ATCC 15,707), Bifidobacterium longum subspecies infantis (ATCC 15,697), and Bifidobacterium animalis subspecies lactis (ATCC 27,673) vs periodontal pathogens: Fusobacterium nucleatum subspecies nucleatum (ATCC 25,585), Porphyromonas gingivalis (33,277), and Streptococcus oralis were grown in brain heart infusion agar , Bacterial strains were incubated for 24 h, 72 h, and 168 h at 37 °C , Checkerboards DNA–DNA hybridisation , The absolute counts of each bacterium in combination were presented in the percentage of bacterial growth.

Techniques: Concentration Assay, In Vitro, Activity Assay, Inhibition, Animal Model, Expressing

Journal: Journal of the International Society of Sports Nutrition

Article Title: “Do probiotics mitigate GI-induced inflammation and perceived fatigue in athletes? A systematic review”

doi: 10.1080/15502783.2024.2388085

Figure Lengend Snippet: Summary of main findings. +, acceptable ROB; ++, high quality/low ROB; -, low quality/high ROB.

Article Snippet: Roberts et al., 2016 , n = 30 (25 M & 5 F) , 3x10(10) LAB(4)Anti 0 billion CFU·day−1 Lactobacillus acidophilus CUL-60 (NCIMB 30,157), 10 billion CFU·day−1 Lactobacillus acidophillus CUL-21 (NCIMB 30,156), 9.5 billion CFU·day−1 Bifidobacterium bifidum CUL-20 (NCIMB 30,172) and 0.5 billion CFU·day−1 Bifidobacterium animalis subspecies lactis CUL-34 (NCIMB 30,153)/55.8 mg·day−1 fructooligosaccharides/400 mg·day−1 α-lipoic acid, 600 mg·day−1 N-acetyl-carnitine). OR LAB(4) containing same strains but not antioxidants , RPE - Results failed to mention outcome , Long distance triathlon. No significant differences , LAB4ANTI significantly reduced endotoxin units , May reduce endotoxin unit levels , (++).

Techniques: Bacteria, Capsules

Journal: Heliyon

Article Title: The effect of probiotic Bifidobacterium lactis Bl-04 on innate antiviral responses in vitro

doi: 10.1016/j.heliyon.2024.e29588

Figure Lengend Snippet: The effect of Bifidobacterium animalis subsp. lactis Bl-04 and pIC + R848 on human macrophage and dendritic cell cytokine responses. Cells were stimulated or left unstimulated with Bl-04 (10 bacteria/cell) and/or pIC (15 μg/ml) + R848 (5 μM) for 24 h (Mf) or 48 h (DC). Cytokine concentrations in the supernatants were measured by ELISA (Mf: A-F; DC: G-N) and the mean of six donors (pg/ml) with standard deviation is shown. DMSO, dimethyl sulfoxide was used as a vehicle control for pIC + R848. The data were analyzed with a linear model using change from baseline as response (Ctrl vs Bl-04; or pIC + R848 vs Bl-04 + pIC + R848). Different comparisons are separated with a dashed line. N = 6, for pIC + R848 technical replicates were used. *: 0.01 ≤ p﹤0.05, **: 0.001 ≤ p﹤0.01, ***: p < 0.001 vs ctrl and: # 0.01 ≤ p﹤0.05, ##: 0.001 ≤ p﹤0.01, ##: p < 0.001 vs pIC + R848.

Article Snippet: To prepare Bl-04™ bacteria for immune cell treatment, Bifidobacterium animalis subspecies lactis Bl-04 (Danisco Global Culture collection DGCC2908 Niebüll, Germany and American Type Culture Collections ATCC SD5219, Manassas, VA, USA) was cultured anaerobically in N2 atmosphere at 37 °C in Bifidobacterium medium 58 (DSMZ, Braunschweig, Germany).

Techniques: Bacteria, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Heliyon

Article Title: The effect of probiotic Bifidobacterium lactis Bl-04 on innate antiviral responses in vitro

doi: 10.1016/j.heliyon.2024.e29588

Figure Lengend Snippet: Principal component analysis of transcriptomes and differentially expressed genes of all samples tested for Mfs and DCs. PCA of all samples (A) Mfs and (B) DCs was performed. Color of the individual points and collective ellipses denotes treatment, shape of the point denotes the human donor of the cells. DEG analysis was done for each treatment. Bl-04 was compared to unstimulated controls and pIC + R848 to DMSO and the number of DEGs for Mfs (C) and DC (D) are shown as Venn diagrams. PC, Principal Component. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: To prepare Bl-04™ bacteria for immune cell treatment, Bifidobacterium animalis subspecies lactis Bl-04 (Danisco Global Culture collection DGCC2908 Niebüll, Germany and American Type Culture Collections ATCC SD5219, Manassas, VA, USA) was cultured anaerobically in N2 atmosphere at 37 °C in Bifidobacterium medium 58 (DSMZ, Braunschweig, Germany).

Techniques:

Journal: Heliyon

Article Title: The effect of probiotic Bifidobacterium lactis Bl-04 on innate antiviral responses in vitro

doi: 10.1016/j.heliyon.2024.e29588

Figure Lengend Snippet: Differential expression of genes involved in antiviral responses in Mfs and DCs after Bifidobacterium animalis subsp. lactis Bl-04 or pIC + R848 treatment. Cells were stimulated with Bl-04 (10 bacteria/cell) or pIC (15 μg/ml) + R848 (5 μM) for 24 h (Mf) or 48 h (DC). RNA was collected and transcriptomic analyses were performed. Selected genes of interest were grouped to (A) Receptor, (B) PRR, (C) Signaling, (D) Interferon, (E) Chemokine, (F) Cytokine and (G) Antiviral protein categories. Color of the balloon denotes expression level (log2 fold change) with blue having reduced expression and red having increased expression compared with the control. Size of the balloon denotes significance (-log10 adjusted p-value, black outline at adjusted p-value <0.05) with the larger size having more significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: To prepare Bl-04™ bacteria for immune cell treatment, Bifidobacterium animalis subspecies lactis Bl-04 (Danisco Global Culture collection DGCC2908 Niebüll, Germany and American Type Culture Collections ATCC SD5219, Manassas, VA, USA) was cultured anaerobically in N2 atmosphere at 37 °C in Bifidobacterium medium 58 (DSMZ, Braunschweig, Germany).

Techniques: Expressing, Bacteria

Journal: Heliyon

Article Title: The effect of probiotic Bifidobacterium lactis Bl-04 on innate antiviral responses in vitro

doi: 10.1016/j.heliyon.2024.e29588

Figure Lengend Snippet: Correlation analysis on the differentially expressed genes. Mfs treated with Bl-04 (A), or pIC + R848 (B), DCs treated with Bl-04 (C), or pIC + R848 (D). The correlation values and the adjusted p-values are visualized in the bubble plots, with the X and Y axes indicating the gene names that are grouped to Receptor, pattern-recognition receptor (PRR), Signaling, Interferon, Chemokine, Cytokine and Antiviral protein categories. The blue and red colors of the balloon indicate a negative and a positive Pearson correlation, respectively. Values with an adjusted p-value <0.05 were circled with a black ring to show significance, and the size of the balloon denotes the -log10 (adjusted p-value). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: To prepare Bl-04™ bacteria for immune cell treatment, Bifidobacterium animalis subspecies lactis Bl-04 (Danisco Global Culture collection DGCC2908 Niebüll, Germany and American Type Culture Collections ATCC SD5219, Manassas, VA, USA) was cultured anaerobically in N2 atmosphere at 37 °C in Bifidobacterium medium 58 (DSMZ, Braunschweig, Germany).

Techniques:

Journal: Heliyon

Article Title: The effect of probiotic Bifidobacterium lactis Bl-04 on innate antiviral responses in vitro

doi: 10.1016/j.heliyon.2024.e29588

Figure Lengend Snippet: The effect of Bifidobacterium animalis subsp. lactis Bl-04 on RV replication in fibroblasts. Mean values (±SEM) of viral replication from low and medium titer (three replicate experiments, target titer 10 1.5 TCID 50 /ml and 10 2.5 TCID 50 /ml) respectively or high titer (target 10 4.5 TCID 50 /ml) experiments are shown. TCID 50 , Median Tissue Culture Infectious Dose. *p = 0.01.

Article Snippet: To prepare Bl-04™ bacteria for immune cell treatment, Bifidobacterium animalis subspecies lactis Bl-04 (Danisco Global Culture collection DGCC2908 Niebüll, Germany and American Type Culture Collections ATCC SD5219, Manassas, VA, USA) was cultured anaerobically in N2 atmosphere at 37 °C in Bifidobacterium medium 58 (DSMZ, Braunschweig, Germany).

Techniques:

Journal: Heliyon

Article Title: The effect of probiotic Bifidobacterium lactis Bl-04 on innate antiviral responses in vitro

doi: 10.1016/j.heliyon.2024.e29588

Figure Lengend Snippet: Cytokine and chemokine production of MRC-5 fibroblast before and after RV infection. (A) IL-6, (B) CXCL8, (C) VEGF, (D) CXCL10, (E) IL-1β. MRC-5 fibroblast cells were treated with Bifidobacterium animalis subsp. lactis Bl-04 (25 bacteria/cell) for 24 h and baseline samples (0 h) were collected. Remaining wells were infected with low (3 experiments), medium (2 experiments) or high titer (1 experiment) of RV for 2 h. Bl-04 treated and control samples were collected after 24, 48 and 72 h of incubation and cytokines were analyzed by ELISA. Error bars represent the SD of three replicate wells. Statistically significant result of Bl-04 versus control is marked with an asterisk (IL-1β high titer 24 h p = 0.037). ND = not detected.

Article Snippet: To prepare Bl-04™ bacteria for immune cell treatment, Bifidobacterium animalis subspecies lactis Bl-04 (Danisco Global Culture collection DGCC2908 Niebüll, Germany and American Type Culture Collections ATCC SD5219, Manassas, VA, USA) was cultured anaerobically in N2 atmosphere at 37 °C in Bifidobacterium medium 58 (DSMZ, Braunschweig, Germany).

Techniques: Infection, Bacteria, Incubation, Enzyme-linked Immunosorbent Assay

Journal: Heliyon

Article Title: The effect of probiotic Bifidobacterium lactis Bl-04 on innate antiviral responses in vitro

doi: 10.1016/j.heliyon.2024.e29588

Figure Lengend Snippet: The effect of Bifidobacterium animalis subsp. lactis Bl-04 and pIC + R848 on human macrophage and dendritic cell cytokine responses. Cells were stimulated or left unstimulated with Bl-04 (10 bacteria/cell) and/or pIC (15 μg/ml) + R848 (5 μM) for 24 h (Mf) or 48 h (DC). Cytokine concentrations in the supernatants were measured by ELISA (Mf: A-F; DC: G-N) and the mean of six donors (pg/ml) with standard deviation is shown. DMSO, dimethyl sulfoxide was used as a vehicle control for pIC + R848. The data were analyzed with a linear model using change from baseline as response (Ctrl vs Bl-04; or pIC + R848 vs Bl-04 + pIC + R848). Different comparisons are separated with a dashed line. N = 6, for pIC + R848 technical replicates were used. *: 0.01 ≤ p﹤0.05, **: 0.001 ≤ p﹤0.01, ***: p < 0.001 vs ctrl and: # 0.01 ≤ p﹤0.05, ##: 0.001 ≤ p﹤0.01, ##: p < 0.001 vs pIC + R848.

Article Snippet: To prepare Bl-04™ bacteria for immune cell treatment, Bifidobacterium animalis subspecies lactis Bl-04 (Danisco Global Culture collection DGCC2908 Niebüll, Germany and American Type Culture Collections ATCC SD5219, Manassas, VA, USA) was cultured anaerobically in N2 atmosphere at 37 °C in Bifidobacterium medium 58 (DSMZ, Braunschweig, Germany).

Techniques: Bacteria, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Heliyon

Article Title: The effect of probiotic Bifidobacterium lactis Bl-04 on innate antiviral responses in vitro

doi: 10.1016/j.heliyon.2024.e29588

Figure Lengend Snippet: Principal component analysis of transcriptomes and differentially expressed genes of all samples tested for Mfs and DCs. PCA of all samples (A) Mfs and (B) DCs was performed. Color of the individual points and collective ellipses denotes treatment, shape of the point denotes the human donor of the cells. DEG analysis was done for each treatment. Bl-04 was compared to unstimulated controls and pIC + R848 to DMSO and the number of DEGs for Mfs (C) and DC (D) are shown as Venn diagrams. PC, Principal Component. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: To prepare Bl-04™ bacteria for immune cell treatment, Bifidobacterium animalis subspecies lactis Bl-04 (Danisco Global Culture collection DGCC2908 Niebüll, Germany and American Type Culture Collections ATCC SD5219, Manassas, VA, USA) was cultured anaerobically in N2 atmosphere at 37 °C in Bifidobacterium medium 58 (DSMZ, Braunschweig, Germany).

Techniques:

Journal: Heliyon

Article Title: The effect of probiotic Bifidobacterium lactis Bl-04 on innate antiviral responses in vitro

doi: 10.1016/j.heliyon.2024.e29588

Figure Lengend Snippet: Differential expression of genes involved in antiviral responses in Mfs and DCs after Bifidobacterium animalis subsp. lactis Bl-04 or pIC + R848 treatment. Cells were stimulated with Bl-04 (10 bacteria/cell) or pIC (15 μg/ml) + R848 (5 μM) for 24 h (Mf) or 48 h (DC). RNA was collected and transcriptomic analyses were performed. Selected genes of interest were grouped to (A) Receptor, (B) PRR, (C) Signaling, (D) Interferon, (E) Chemokine, (F) Cytokine and (G) Antiviral protein categories. Color of the balloon denotes expression level (log2 fold change) with blue having reduced expression and red having increased expression compared with the control. Size of the balloon denotes significance (-log10 adjusted p-value, black outline at adjusted p-value <0.05) with the larger size having more significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: To prepare Bl-04™ bacteria for immune cell treatment, Bifidobacterium animalis subspecies lactis Bl-04 (Danisco Global Culture collection DGCC2908 Niebüll, Germany and American Type Culture Collections ATCC SD5219, Manassas, VA, USA) was cultured anaerobically in N2 atmosphere at 37 °C in Bifidobacterium medium 58 (DSMZ, Braunschweig, Germany).

Techniques: Expressing, Bacteria

Journal: Heliyon

Article Title: The effect of probiotic Bifidobacterium lactis Bl-04 on innate antiviral responses in vitro

doi: 10.1016/j.heliyon.2024.e29588

Figure Lengend Snippet: Correlation analysis on the differentially expressed genes. Mfs treated with Bl-04 (A), or pIC + R848 (B), DCs treated with Bl-04 (C), or pIC + R848 (D). The correlation values and the adjusted p-values are visualized in the bubble plots, with the X and Y axes indicating the gene names that are grouped to Receptor, pattern-recognition receptor (PRR), Signaling, Interferon, Chemokine, Cytokine and Antiviral protein categories. The blue and red colors of the balloon indicate a negative and a positive Pearson correlation, respectively. Values with an adjusted p-value <0.05 were circled with a black ring to show significance, and the size of the balloon denotes the -log10 (adjusted p-value). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: To prepare Bl-04™ bacteria for immune cell treatment, Bifidobacterium animalis subspecies lactis Bl-04 (Danisco Global Culture collection DGCC2908 Niebüll, Germany and American Type Culture Collections ATCC SD5219, Manassas, VA, USA) was cultured anaerobically in N2 atmosphere at 37 °C in Bifidobacterium medium 58 (DSMZ, Braunschweig, Germany).

Techniques:

Journal: Heliyon

Article Title: The effect of probiotic Bifidobacterium lactis Bl-04 on innate antiviral responses in vitro

doi: 10.1016/j.heliyon.2024.e29588

Figure Lengend Snippet: The effect of Bifidobacterium animalis subsp. lactis Bl-04 on RV replication in fibroblasts. Mean values (±SEM) of viral replication from low and medium titer (three replicate experiments, target titer 10 1.5 TCID 50 /ml and 10 2.5 TCID 50 /ml) respectively or high titer (target 10 4.5 TCID 50 /ml) experiments are shown. TCID 50 , Median Tissue Culture Infectious Dose. *p = 0.01.

Article Snippet: To prepare Bl-04™ bacteria for immune cell treatment, Bifidobacterium animalis subspecies lactis Bl-04 (Danisco Global Culture collection DGCC2908 Niebüll, Germany and American Type Culture Collections ATCC SD5219, Manassas, VA, USA) was cultured anaerobically in N2 atmosphere at 37 °C in Bifidobacterium medium 58 (DSMZ, Braunschweig, Germany).

Techniques:

Journal: Heliyon

Article Title: The effect of probiotic Bifidobacterium lactis Bl-04 on innate antiviral responses in vitro

doi: 10.1016/j.heliyon.2024.e29588

Figure Lengend Snippet: Cytokine and chemokine production of MRC-5 fibroblast before and after RV infection. (A) IL-6, (B) CXCL8, (C) VEGF, (D) CXCL10, (E) IL-1β. MRC-5 fibroblast cells were treated with Bifidobacterium animalis subsp. lactis Bl-04 (25 bacteria/cell) for 24 h and baseline samples (0 h) were collected. Remaining wells were infected with low (3 experiments), medium (2 experiments) or high titer (1 experiment) of RV for 2 h. Bl-04 treated and control samples were collected after 24, 48 and 72 h of incubation and cytokines were analyzed by ELISA. Error bars represent the SD of three replicate wells. Statistically significant result of Bl-04 versus control is marked with an asterisk (IL-1β high titer 24 h p = 0.037). ND = not detected.

Article Snippet: To prepare Bl-04™ bacteria for immune cell treatment, Bifidobacterium animalis subspecies lactis Bl-04 (Danisco Global Culture collection DGCC2908 Niebüll, Germany and American Type Culture Collections ATCC SD5219, Manassas, VA, USA) was cultured anaerobically in N2 atmosphere at 37 °C in Bifidobacterium medium 58 (DSMZ, Braunschweig, Germany).

Techniques: Infection, Bacteria, Incubation, Enzyme-linked Immunosorbent Assay