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bifidobacterium animalis subsp lactis atcc 27673  (ATCC)


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    ATCC bifidobacterium animalis subsp lactis atcc 27673
    Growth and production of short-chain fatty acids (SCFAs) during cultivation of <t>Bifidobacterium</t> adolescentis (BA) and Roseburia hominis (RH) on (galacto)-β-mannan-oligosaccharide (MOS/GMOS). ( A ) Cell concentration by qPCR-analysis for BA and RH in mono- and cocultures. ( B ) Relative strain abundance (%) in the coculture. ( C ) The BA monocultivation (in the medium for colon bacteria, MCB). ( D ) The RH monocultivation (in the modified medium for colon bacteria, mMCB). ( E ) The cocultivation of RH and BA in MCB. Rec A (for BA) and rho (for RH) genes were used as markers in the qPCR-analysis to determine the cell concentration, expressed as log (copy number/mL) in the mono- and cocultures. The sum of calculated gene-copy-number values was used to determine the relative population (%) of RH and BA in the cocultures. The optical density (OD) was determined at 600 nm. The amount of SCFAs was determined by HPLC.
    Bifidobacterium Animalis Subsp Lactis Atcc 27673, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Cross-Feeding and Enzymatic Catabolism for Mannan-Oligosaccharide Utilization by the Butyrate-Producing Gut Bacterium Roseburia hominis A2-183"

    Article Title: Cross-Feeding and Enzymatic Catabolism for Mannan-Oligosaccharide Utilization by the Butyrate-Producing Gut Bacterium Roseburia hominis A2-183

    Journal: Microorganisms

    doi: 10.3390/microorganisms10122496

    Growth and production of short-chain fatty acids (SCFAs) during cultivation of Bifidobacterium adolescentis (BA) and Roseburia hominis (RH) on (galacto)-β-mannan-oligosaccharide (MOS/GMOS). ( A ) Cell concentration by qPCR-analysis for BA and RH in mono- and cocultures. ( B ) Relative strain abundance (%) in the coculture. ( C ) The BA monocultivation (in the medium for colon bacteria, MCB). ( D ) The RH monocultivation (in the modified medium for colon bacteria, mMCB). ( E ) The cocultivation of RH and BA in MCB. Rec A (for BA) and rho (for RH) genes were used as markers in the qPCR-analysis to determine the cell concentration, expressed as log (copy number/mL) in the mono- and cocultures. The sum of calculated gene-copy-number values was used to determine the relative population (%) of RH and BA in the cocultures. The optical density (OD) was determined at 600 nm. The amount of SCFAs was determined by HPLC.
    Figure Legend Snippet: Growth and production of short-chain fatty acids (SCFAs) during cultivation of Bifidobacterium adolescentis (BA) and Roseburia hominis (RH) on (galacto)-β-mannan-oligosaccharide (MOS/GMOS). ( A ) Cell concentration by qPCR-analysis for BA and RH in mono- and cocultures. ( B ) Relative strain abundance (%) in the coculture. ( C ) The BA monocultivation (in the medium for colon bacteria, MCB). ( D ) The RH monocultivation (in the modified medium for colon bacteria, mMCB). ( E ) The cocultivation of RH and BA in MCB. Rec A (for BA) and rho (for RH) genes were used as markers in the qPCR-analysis to determine the cell concentration, expressed as log (copy number/mL) in the mono- and cocultures. The sum of calculated gene-copy-number values was used to determine the relative population (%) of RH and BA in the cocultures. The optical density (OD) was determined at 600 nm. The amount of SCFAs was determined by HPLC.

    Techniques Used: Concentration Assay, Modification



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    Growth and production of short-chain fatty acids (SCFAs) during cultivation of <t>Bifidobacterium</t> adolescentis (BA) and Roseburia hominis (RH) on (galacto)-β-mannan-oligosaccharide (MOS/GMOS). ( A ) Cell concentration by qPCR-analysis for BA and RH in mono- and cocultures. ( B ) Relative strain abundance (%) in the coculture. ( C ) The BA monocultivation (in the medium for colon bacteria, MCB). ( D ) The RH monocultivation (in the modified medium for colon bacteria, mMCB). ( E ) The cocultivation of RH and BA in MCB. Rec A (for BA) and rho (for RH) genes were used as markers in the qPCR-analysis to determine the cell concentration, expressed as log (copy number/mL) in the mono- and cocultures. The sum of calculated gene-copy-number values was used to determine the relative population (%) of RH and BA in the cocultures. The optical density (OD) was determined at 600 nm. The amount of SCFAs was determined by HPLC.
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    Image Search Results


    Growth and production of short-chain fatty acids (SCFAs) during cultivation of Bifidobacterium adolescentis (BA) and Roseburia hominis (RH) on (galacto)-β-mannan-oligosaccharide (MOS/GMOS). ( A ) Cell concentration by qPCR-analysis for BA and RH in mono- and cocultures. ( B ) Relative strain abundance (%) in the coculture. ( C ) The BA monocultivation (in the medium for colon bacteria, MCB). ( D ) The RH monocultivation (in the modified medium for colon bacteria, mMCB). ( E ) The cocultivation of RH and BA in MCB. Rec A (for BA) and rho (for RH) genes were used as markers in the qPCR-analysis to determine the cell concentration, expressed as log (copy number/mL) in the mono- and cocultures. The sum of calculated gene-copy-number values was used to determine the relative population (%) of RH and BA in the cocultures. The optical density (OD) was determined at 600 nm. The amount of SCFAs was determined by HPLC.

    Journal: Microorganisms

    Article Title: Cross-Feeding and Enzymatic Catabolism for Mannan-Oligosaccharide Utilization by the Butyrate-Producing Gut Bacterium Roseburia hominis A2-183

    doi: 10.3390/microorganisms10122496

    Figure Lengend Snippet: Growth and production of short-chain fatty acids (SCFAs) during cultivation of Bifidobacterium adolescentis (BA) and Roseburia hominis (RH) on (galacto)-β-mannan-oligosaccharide (MOS/GMOS). ( A ) Cell concentration by qPCR-analysis for BA and RH in mono- and cocultures. ( B ) Relative strain abundance (%) in the coculture. ( C ) The BA monocultivation (in the medium for colon bacteria, MCB). ( D ) The RH monocultivation (in the modified medium for colon bacteria, mMCB). ( E ) The cocultivation of RH and BA in MCB. Rec A (for BA) and rho (for RH) genes were used as markers in the qPCR-analysis to determine the cell concentration, expressed as log (copy number/mL) in the mono- and cocultures. The sum of calculated gene-copy-number values was used to determine the relative population (%) of RH and BA in the cocultures. The optical density (OD) was determined at 600 nm. The amount of SCFAs was determined by HPLC.

    Article Snippet: The top hit for Rh MosBP was a mannan-oligosaccharide binding protein ( Bl MnBP1) of an ABC-transporter from Bifidobacterium animalis subsp. lactis ATCC 27673 [ ] ( ).

    Techniques: Concentration Assay, Modification

    Bifidobacterium species included in the study <xref ref-type= a " width="100%" height="100%">

    Journal: Applied and Environmental Microbiology

    Article Title: Genome-Wide Assessment of Stress-Associated Genes in Bifidobacteria

    doi: 10.1128/aem.02251-21

    Figure Lengend Snippet: Bifidobacterium species included in the study a

    Article Snippet: Strains lacking homologs were strains of the B. asteroides group, B. subtile , B. eulemuris DSM 100216 (only representative of B. eulemuris species), B. longum BORI and CCUG 30698, and B. animalis subsp. lactis ATCC 27673, which has already been described as having a genetic content distinct from that of other strains of the species ( ).

    Techniques: Isolation

    Identification of HemW-specific motifs in HemN homologs of B. animalis BB-12, B. longum NCC2705, and B. asteroides PRL2011. To assess the presence of HemW-specific motifs in the protein sequences of HemN homologs from B. animalis subsp. lactis BB-12 ( WP_004217870.1 ), B. longum subsp. longum NCC2705 ( WP_008783712.1 ), and B. asteroides PRL2011 ( WP_033511215.1 ), their protein sequences were aligned with HemW of L. lactis ( WP_003132086.1 ). The alignment was generated using CLC Genomics Workbench 20.0, default settings, alignment mode set to very accurate. Blue boxes are HemW-specific conserved amino acid residues that distinguish the protein from HemN, identified in L. lactis : H 134 , H 184 xxxYxLxxE, Y 234 ExS, and especially H 248 NxxYW .

    Journal: Applied and Environmental Microbiology

    Article Title: Genome-Wide Assessment of Stress-Associated Genes in Bifidobacteria

    doi: 10.1128/aem.02251-21

    Figure Lengend Snippet: Identification of HemW-specific motifs in HemN homologs of B. animalis BB-12, B. longum NCC2705, and B. asteroides PRL2011. To assess the presence of HemW-specific motifs in the protein sequences of HemN homologs from B. animalis subsp. lactis BB-12 ( WP_004217870.1 ), B. longum subsp. longum NCC2705 ( WP_008783712.1 ), and B. asteroides PRL2011 ( WP_033511215.1 ), their protein sequences were aligned with HemW of L. lactis ( WP_003132086.1 ). The alignment was generated using CLC Genomics Workbench 20.0, default settings, alignment mode set to very accurate. Blue boxes are HemW-specific conserved amino acid residues that distinguish the protein from HemN, identified in L. lactis : H 134 , H 184 xxxYxLxxE, Y 234 ExS, and especially H 248 NxxYW .

    Article Snippet: Strains lacking homologs were strains of the B. asteroides group, B. subtile , B. eulemuris DSM 100216 (only representative of B. eulemuris species), B. longum BORI and CCUG 30698, and B. animalis subsp. lactis ATCC 27673, which has already been described as having a genetic content distinct from that of other strains of the species ( ).

    Techniques: Generated

    Active-site motif of BCP homologs in Bifidobacterium strains. ★, peroxidatic cysteine; ▾, resolving cysteine, which is absent from BCPs of B. animalis .

    Journal: Applied and Environmental Microbiology

    Article Title: Genome-Wide Assessment of Stress-Associated Genes in Bifidobacteria

    doi: 10.1128/aem.02251-21

    Figure Lengend Snippet: Active-site motif of BCP homologs in Bifidobacterium strains. ★, peroxidatic cysteine; ▾, resolving cysteine, which is absent from BCPs of B. animalis .

    Article Snippet: Strains lacking homologs were strains of the B. asteroides group, B. subtile , B. eulemuris DSM 100216 (only representative of B. eulemuris species), B. longum BORI and CCUG 30698, and B. animalis subsp. lactis ATCC 27673, which has already been described as having a genetic content distinct from that of other strains of the species ( ).

    Techniques: