Structured Review

Roche bglii
Oligonucleotide-modified plasmid assay. (A) Plasmid modification scheme. <t>pTW423</t> is digested with <t>BglII</t> and XhoI and purified, removing the polyterminator. Annealed oligonucleotides are then ligated onto the BglII and XhoI ends, restoring the ADE2 coding sequence. Precise in-frame repair of the break yields Ade + colonies. (B) Primer extension assay to determine the oligonucleotide ligation efficiency. Annealed oligonucleotides (Oligos) were added to the ligation reaction mixture at concentrations of 50, 100, 500, 1,000, and 5,000-fold molar excess over the concentration of the linearized plasmid (indicated by the thickness of the triangle over the lanes). Primer extension was performed after ligation as described in Materials and Methods.
Bglii, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 17 article reviews
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bglii - by Bioz Stars, 2020-07
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Images

1) Product Images from "Rejoining of DNA Double-Strand Breaks as a Function of Overhang Length †"

Article Title: Rejoining of DNA Double-Strand Breaks as a Function of Overhang Length †

Journal: Molecular and Cellular Biology

doi: 10.1128/MCB.25.3.896-906.2005

Oligonucleotide-modified plasmid assay. (A) Plasmid modification scheme. pTW423 is digested with BglII and XhoI and purified, removing the polyterminator. Annealed oligonucleotides are then ligated onto the BglII and XhoI ends, restoring the ADE2 coding sequence. Precise in-frame repair of the break yields Ade + colonies. (B) Primer extension assay to determine the oligonucleotide ligation efficiency. Annealed oligonucleotides (Oligos) were added to the ligation reaction mixture at concentrations of 50, 100, 500, 1,000, and 5,000-fold molar excess over the concentration of the linearized plasmid (indicated by the thickness of the triangle over the lanes). Primer extension was performed after ligation as described in Materials and Methods.
Figure Legend Snippet: Oligonucleotide-modified plasmid assay. (A) Plasmid modification scheme. pTW423 is digested with BglII and XhoI and purified, removing the polyterminator. Annealed oligonucleotides are then ligated onto the BglII and XhoI ends, restoring the ADE2 coding sequence. Precise in-frame repair of the break yields Ade + colonies. (B) Primer extension assay to determine the oligonucleotide ligation efficiency. Annealed oligonucleotides (Oligos) were added to the ligation reaction mixture at concentrations of 50, 100, 500, 1,000, and 5,000-fold molar excess over the concentration of the linearized plasmid (indicated by the thickness of the triangle over the lanes). Primer extension was performed after ligation as described in Materials and Methods.

Techniques Used: Modification, Plasmid Preparation, Purification, Sequencing, Primer Extension Assay, Ligation, Concentration Assay

2) Product Images from "Requirement of the galU Gene for Polysaccharide Production by and Pathogenicity and Growth In Planta of Xanthomonas citri subsp. citri ▿"

Article Title: Requirement of the galU Gene for Polysaccharide Production by and Pathogenicity and Growth In Planta of Xanthomonas citri subsp. citri ▿

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.02897-09

Sequence analysis of EZ-Tn5 insertion in the galU mutants. (A) Genomic location of galU on the X. citri subsp. citri chromosome and transposon insertion sites in the galU mutants. kefB encodes a transport protein, galU encodes a UTP-glucose-1-phosphate uridylyltransferase, and XCC2293 encodes a dehydratase protein. Bg, BglII restriction site. (B) PCR analysis confirming insertion of EZ-Tn5 into the galU gene: agarose gel electrophoresis of DNA amplified using primers galU -F1 and galU -R1 targeting the interior region of the galU gene from the X. citri subsp. citri wild-type 306, D12, and F6 strains. Lane 1, Invitrogen 1 Kb Plus DNA size marker; lane 2, D12, lane 3, F6, lane 4, X. citri subsp. citri 306. (C) Southern blot of DNA of X. citri subsp. citri wild-type strain 306 and galU mutants F6 and D12 digested with BglII. The membrane was probed with a 675-bp kan-2 gene fragment that was amplified using PCR with primers Kan-F1 and Kan-R1. Wt, wild type.
Figure Legend Snippet: Sequence analysis of EZ-Tn5 insertion in the galU mutants. (A) Genomic location of galU on the X. citri subsp. citri chromosome and transposon insertion sites in the galU mutants. kefB encodes a transport protein, galU encodes a UTP-glucose-1-phosphate uridylyltransferase, and XCC2293 encodes a dehydratase protein. Bg, BglII restriction site. (B) PCR analysis confirming insertion of EZ-Tn5 into the galU gene: agarose gel electrophoresis of DNA amplified using primers galU -F1 and galU -R1 targeting the interior region of the galU gene from the X. citri subsp. citri wild-type 306, D12, and F6 strains. Lane 1, Invitrogen 1 Kb Plus DNA size marker; lane 2, D12, lane 3, F6, lane 4, X. citri subsp. citri 306. (C) Southern blot of DNA of X. citri subsp. citri wild-type strain 306 and galU mutants F6 and D12 digested with BglII. The membrane was probed with a 675-bp kan-2 gene fragment that was amplified using PCR with primers Kan-F1 and Kan-R1. Wt, wild type.

Techniques Used: Sequencing, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Amplification, Marker, Southern Blot

3) Product Images from "Restriction site tagged (RST) microarrays: a novel technique to study the species composition of complex microbial systems"

Article Title: Restriction site tagged (RST) microarrays: a novel technique to study the species composition of complex microbial systems

Journal: Nucleic Acids Research

doi:

Hybridization of NR probe prepared from bacterial mixtures to NotI microarrays. DNA from E.coli R2 (A), E.cloacae R4 (B), K.pneumonia B4958 (C) and S.liquefaciens ATCC14460 (D) were mixed in different proportions (shown on the right) and a NR probe was prepared using BamHI and BglII digestion. NotI linking clones selected from S.liquefaciens , K.pneumoniae B4958, E.cloaceae R4 and E.coli R2 and shown at the bottom were used for the microarrays.
Figure Legend Snippet: Hybridization of NR probe prepared from bacterial mixtures to NotI microarrays. DNA from E.coli R2 (A), E.cloacae R4 (B), K.pneumonia B4958 (C) and S.liquefaciens ATCC14460 (D) were mixed in different proportions (shown on the right) and a NR probe was prepared using BamHI and BglII digestion. NotI linking clones selected from S.liquefaciens , K.pneumoniae B4958, E.cloaceae R4 and E.coli R2 and shown at the bottom were used for the microarrays.

Techniques Used: Hybridization, Clone Assay

Related Articles

Electroporation:

Article Title: Saturation mutagenesis of selected residues of the ?-peptide of the lantibiotic lacticin 3147 yields a derivative with enhanced antimicrobial activity
Article Snippet: .. Amplified products were purified as before, digested with BglII and XbaI (Roche), ligated with similarly digested and shrimp alkaline phosphatase (Fermentas)-treated pPTPL and introduced by electroporation into E. coli MC1000. ..

Amplification:

Article Title: Optimizing microarray-based in situ transcription-translation of proteins for MALDI mass spectrometry
Article Snippet: .. The sGFP-AviTag sequence was amplified with primers 5′-CTGACTTCCGGAATGGTGAGCAAGGGCGAGG -3′ (BspE I, forward) and 5′-ACTGAAGATCTTTATTCGTGCCATTCGATTTTC -3′ (Bgl II, reverse), the product linearized with BspEI and BglII, and ligated into the XmaI/BamHI sites of the pIVEX2.3d vector (Roche). .. This approach maintains the pIVEX2.3d multiple cloning site in an almost intact state, which enhances its suitability for production of in-frame C-terminus GFP-tagged proteins.

Article Title: Saturation mutagenesis of selected residues of the ?-peptide of the lantibiotic lacticin 3147 yields a derivative with enhanced antimicrobial activity
Article Snippet: .. Amplified products were purified as before, digested with BglII and XbaI (Roche), ligated with similarly digested and shrimp alkaline phosphatase (Fermentas)-treated pPTPL and introduced by electroporation into E. coli MC1000. ..

Agarose Gel Electrophoresis:

Article Title: Requirement of the galU Gene for Polysaccharide Production by and Pathogenicity and Growth In Planta of Xanthomonas citri subsp. citri ▿
Article Snippet: .. Genomic DNA (3 μg per sample) was digested with BglII, subjected to electrophoresis on a 0.9% agarose gel, and transferred to a positively charged nylon membrane (Roche, Indianapolis, IN) using standard procedures ( ). .. Probe generation, hybridization, and detection of chemiluminescence were performed using a digoxigenin (DIG)-High Prime II DNA labeling and detection starter kit as recommended by the manufacturer (Roche, Indianapolis, IN).

Southern Blot:

Article Title: Factor-induced Reprogramming and Zinc Finger Nuclease-aided Gene Targeting Cause Different Genome Instability in β-Thalassemia Induced Pluripotent Stem Cells (iPSCs) *
Article Snippet: .. Genomic DNA was digested by BglII, and then standard Southern blotting was performed following the instructions of DIG High Prime DNA Labeling and Detection Starter kit II (Roche Applied Science). .. Cells were digested by 0.25% trypsin (Invitrogen) and fixed with 1% paraformaldehyde for 10 min at 37 °C.

Purification:

Article Title: Rejoining of DNA Double-Strand Breaks as a Function of Overhang Length †
Article Snippet: .. Annealed oligonucleotides (200 pmol) were ligated onto 4 pmol of pTW423 digested with BglII and XhoI with T4 DNA ligase (Roche Molecular Biochemicals) at 16°C for 16 h. Plasmids were then purified from unligated oligonucleotides with the GeneClean kit (QBioGene). .. DNA concentration was quantified by UV spectrometry, and product size, linearity, and integrity were verified by agarose gel electrophoresis.

Article Title: Saturation mutagenesis of selected residues of the ?-peptide of the lantibiotic lacticin 3147 yields a derivative with enhanced antimicrobial activity
Article Snippet: .. Amplified products were purified as before, digested with BglII and XbaI (Roche), ligated with similarly digested and shrimp alkaline phosphatase (Fermentas)-treated pPTPL and introduced by electroporation into E. coli MC1000. ..

Electrophoresis:

Article Title: Requirement of the galU Gene for Polysaccharide Production by and Pathogenicity and Growth In Planta of Xanthomonas citri subsp. citri ▿
Article Snippet: .. Genomic DNA (3 μg per sample) was digested with BglII, subjected to electrophoresis on a 0.9% agarose gel, and transferred to a positively charged nylon membrane (Roche, Indianapolis, IN) using standard procedures ( ). .. Probe generation, hybridization, and detection of chemiluminescence were performed using a digoxigenin (DIG)-High Prime II DNA labeling and detection starter kit as recommended by the manufacturer (Roche, Indianapolis, IN).

DNA Labeling:

Article Title: Factor-induced Reprogramming and Zinc Finger Nuclease-aided Gene Targeting Cause Different Genome Instability in β-Thalassemia Induced Pluripotent Stem Cells (iPSCs) *
Article Snippet: .. Genomic DNA was digested by BglII, and then standard Southern blotting was performed following the instructions of DIG High Prime DNA Labeling and Detection Starter kit II (Roche Applied Science). .. Cells were digested by 0.25% trypsin (Invitrogen) and fixed with 1% paraformaldehyde for 10 min at 37 °C.

Sequencing:

Article Title: Optimizing microarray-based in situ transcription-translation of proteins for MALDI mass spectrometry
Article Snippet: .. The sGFP-AviTag sequence was amplified with primers 5′-CTGACTTCCGGAATGGTGAGCAAGGGCGAGG -3′ (BspE I, forward) and 5′-ACTGAAGATCTTTATTCGTGCCATTCGATTTTC -3′ (Bgl II, reverse), the product linearized with BspEI and BglII, and ligated into the XmaI/BamHI sites of the pIVEX2.3d vector (Roche). .. This approach maintains the pIVEX2.3d multiple cloning site in an almost intact state, which enhances its suitability for production of in-frame C-terminus GFP-tagged proteins.

Plasmid Preparation:

Article Title: Optimizing microarray-based in situ transcription-translation of proteins for MALDI mass spectrometry
Article Snippet: .. The sGFP-AviTag sequence was amplified with primers 5′-CTGACTTCCGGAATGGTGAGCAAGGGCGAGG -3′ (BspE I, forward) and 5′-ACTGAAGATCTTTATTCGTGCCATTCGATTTTC -3′ (Bgl II, reverse), the product linearized with BspEI and BglII, and ligated into the XmaI/BamHI sites of the pIVEX2.3d vector (Roche). .. This approach maintains the pIVEX2.3d multiple cloning site in an almost intact state, which enhances its suitability for production of in-frame C-terminus GFP-tagged proteins.

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    Roche bglii
    Oligonucleotide-modified plasmid assay. (A) Plasmid modification scheme. <t>pTW423</t> is digested with <t>BglII</t> and XhoI and purified, removing the polyterminator. Annealed oligonucleotides are then ligated onto the BglII and XhoI ends, restoring the ADE2 coding sequence. Precise in-frame repair of the break yields Ade + colonies. (B) Primer extension assay to determine the oligonucleotide ligation efficiency. Annealed oligonucleotides (Oligos) were added to the ligation reaction mixture at concentrations of 50, 100, 500, 1,000, and 5,000-fold molar excess over the concentration of the linearized plasmid (indicated by the thickness of the triangle over the lanes). Primer extension was performed after ligation as described in Materials and Methods.
    Bglii, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii/product/Roche
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    bglii - by Bioz Stars, 2020-07
    92/100 stars
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    Oligonucleotide-modified plasmid assay. (A) Plasmid modification scheme. pTW423 is digested with BglII and XhoI and purified, removing the polyterminator. Annealed oligonucleotides are then ligated onto the BglII and XhoI ends, restoring the ADE2 coding sequence. Precise in-frame repair of the break yields Ade + colonies. (B) Primer extension assay to determine the oligonucleotide ligation efficiency. Annealed oligonucleotides (Oligos) were added to the ligation reaction mixture at concentrations of 50, 100, 500, 1,000, and 5,000-fold molar excess over the concentration of the linearized plasmid (indicated by the thickness of the triangle over the lanes). Primer extension was performed after ligation as described in Materials and Methods.

    Journal: Molecular and Cellular Biology

    Article Title: Rejoining of DNA Double-Strand Breaks as a Function of Overhang Length †

    doi: 10.1128/MCB.25.3.896-906.2005

    Figure Lengend Snippet: Oligonucleotide-modified plasmid assay. (A) Plasmid modification scheme. pTW423 is digested with BglII and XhoI and purified, removing the polyterminator. Annealed oligonucleotides are then ligated onto the BglII and XhoI ends, restoring the ADE2 coding sequence. Precise in-frame repair of the break yields Ade + colonies. (B) Primer extension assay to determine the oligonucleotide ligation efficiency. Annealed oligonucleotides (Oligos) were added to the ligation reaction mixture at concentrations of 50, 100, 500, 1,000, and 5,000-fold molar excess over the concentration of the linearized plasmid (indicated by the thickness of the triangle over the lanes). Primer extension was performed after ligation as described in Materials and Methods.

    Article Snippet: Annealed oligonucleotides (200 pmol) were ligated onto 4 pmol of pTW423 digested with BglII and XhoI with T4 DNA ligase (Roche Molecular Biochemicals) at 16°C for 16 h. Plasmids were then purified from unligated oligonucleotides with the GeneClean kit (QBioGene).

    Techniques: Modification, Plasmid Preparation, Purification, Sequencing, Primer Extension Assay, Ligation, Concentration Assay

    Enhancer E forms close contact with Myc promoter in 3C assay. ( A ) The 3C primer locations. ( B ) PCR results in the presence or absence of β-catenin and/or Tcf-4 by using the indicated amounts of LnCAP BglII digested/ligated genomic DNA or nondigested/ligated

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Long-range enhancers on 8q24 regulate c-Myc

    doi: 10.1073/pnas.0906067107

    Figure Lengend Snippet: Enhancer E forms close contact with Myc promoter in 3C assay. ( A ) The 3C primer locations. ( B ) PCR results in the presence or absence of β-catenin and/or Tcf-4 by using the indicated amounts of LnCAP BglII digested/ligated genomic DNA or nondigested/ligated

    Article Snippet: Concentration of template DNA was assessed with SYBR green qPCR by using GAPDH primers internal to BglII sites in comparison with Roche human DNA.

    Techniques: Polymerase Chain Reaction

    Sequence analysis of EZ-Tn5 insertion in the galU mutants. (A) Genomic location of galU on the X. citri subsp. citri chromosome and transposon insertion sites in the galU mutants. kefB encodes a transport protein, galU encodes a UTP-glucose-1-phosphate uridylyltransferase, and XCC2293 encodes a dehydratase protein. Bg, BglII restriction site. (B) PCR analysis confirming insertion of EZ-Tn5 into the galU gene: agarose gel electrophoresis of DNA amplified using primers galU -F1 and galU -R1 targeting the interior region of the galU gene from the X. citri subsp. citri wild-type 306, D12, and F6 strains. Lane 1, Invitrogen 1 Kb Plus DNA size marker; lane 2, D12, lane 3, F6, lane 4, X. citri subsp. citri 306. (C) Southern blot of DNA of X. citri subsp. citri wild-type strain 306 and galU mutants F6 and D12 digested with BglII. The membrane was probed with a 675-bp kan-2 gene fragment that was amplified using PCR with primers Kan-F1 and Kan-R1. Wt, wild type.

    Journal: Applied and Environmental Microbiology

    Article Title: Requirement of the galU Gene for Polysaccharide Production by and Pathogenicity and Growth In Planta of Xanthomonas citri subsp. citri ▿

    doi: 10.1128/AEM.02897-09

    Figure Lengend Snippet: Sequence analysis of EZ-Tn5 insertion in the galU mutants. (A) Genomic location of galU on the X. citri subsp. citri chromosome and transposon insertion sites in the galU mutants. kefB encodes a transport protein, galU encodes a UTP-glucose-1-phosphate uridylyltransferase, and XCC2293 encodes a dehydratase protein. Bg, BglII restriction site. (B) PCR analysis confirming insertion of EZ-Tn5 into the galU gene: agarose gel electrophoresis of DNA amplified using primers galU -F1 and galU -R1 targeting the interior region of the galU gene from the X. citri subsp. citri wild-type 306, D12, and F6 strains. Lane 1, Invitrogen 1 Kb Plus DNA size marker; lane 2, D12, lane 3, F6, lane 4, X. citri subsp. citri 306. (C) Southern blot of DNA of X. citri subsp. citri wild-type strain 306 and galU mutants F6 and D12 digested with BglII. The membrane was probed with a 675-bp kan-2 gene fragment that was amplified using PCR with primers Kan-F1 and Kan-R1. Wt, wild type.

    Article Snippet: Genomic DNA (3 μg per sample) was digested with BglII, subjected to electrophoresis on a 0.9% agarose gel, and transferred to a positively charged nylon membrane (Roche, Indianapolis, IN) using standard procedures ( ).

    Techniques: Sequencing, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Amplification, Marker, Southern Blot

    Hybridization of NR probe prepared from bacterial mixtures to NotI microarrays. DNA from E.coli R2 (A), E.cloacae R4 (B), K.pneumonia B4958 (C) and S.liquefaciens ATCC14460 (D) were mixed in different proportions (shown on the right) and a NR probe was prepared using BamHI and BglII digestion. NotI linking clones selected from S.liquefaciens , K.pneumoniae B4958, E.cloaceae R4 and E.coli R2 and shown at the bottom were used for the microarrays.

    Journal: Nucleic Acids Research

    Article Title: Restriction site tagged (RST) microarrays: a novel technique to study the species composition of complex microbial systems

    doi:

    Figure Lengend Snippet: Hybridization of NR probe prepared from bacterial mixtures to NotI microarrays. DNA from E.coli R2 (A), E.cloacae R4 (B), K.pneumonia B4958 (C) and S.liquefaciens ATCC14460 (D) were mixed in different proportions (shown on the right) and a NR probe was prepared using BamHI and BglII digestion. NotI linking clones selected from S.liquefaciens , K.pneumoniae B4958, E.cloaceae R4 and E.coli R2 and shown at the bottom were used for the microarrays.

    Article Snippet: An aliquot of 2 µg of bacterial DNA was digested with 20 U BamHI and 20 U BglII (Roche Molecular Biochemicals) at 37°C for 5 h and then heat-inactivated for 20 min at 85°C.

    Techniques: Hybridization, Clone Assay