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Evrogen bglii
Bglii, supplied by Evrogen, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bglii/product/Evrogen
Average 93 stars, based on 13 article reviews
Price from $9.99 to $1999.99
bglii - by Bioz Stars, 2020-07
93/100 stars

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Clone Assay:

Article Title: UDP-N-acetylglucosamine Transporter (SLC35A3) Regulates Biosynthesis of Highly Branched N-Glycans and Keratan Sulfate
Article Snippet: .. ORF of human UDP-xylose/ N -acetylglucosamine transporter (SLC35B4; ) with BglII and BamHI restriction sites at both ends was amplified using cDNA synthesized from total RNA isolated from HeLa cells and subsequently cloned into pTagGFP2-C vector (Evrogen). .. All ligations were performed using a rapid DNA ligation kit (Fermentas).

Article Title: Spatiotemporal control of phosphatidylinositol 4-phosphate by Sac2 regulates endocytic recycling
Article Snippet: .. The gene encoding human clathrin light chain (aa 1–248) was inserted using NheI and BglII in frame with the N-terminal TagRFP (Evrogen) into the C1 cloning vectors (Takara Bio Inc.; ). .. The PI(4)P probe P4C was cloned from Legionella pneumophila SidC (aa 614–744) and inserted into pEGFP-C1.

Article Title: Actin Depletion Initiates Events Leading to Granule Secretion at the Immunological Synapse
Article Snippet: .. For CD3ζ-EGFP, full-length murine CD247 amplified by PCR from OT-I CTL cDNA was cloned into the XhoI and SalI sites of pEGFP-C1 (Clontech); for PACT-TagBFP, PACT ( ) was cloned into the BglII and XbaI sites of pTagBFP-C (Evrogen); for EGFP-MAPTau, MAPTau was subcloned into the XhoI and BamHI sites of pEGFP-N1; and for TagRFP-MEM-pMig, TagRFP was substituted in EYFP-MEM (Clontech) and Farnesyl-TagBFP2-pMig, which were subcloned into a pMig-R1 retroviral vector. .. Lifeact-mApple, Lifeact-mEmerald, mTagBFP2-MAPTau-N10, and mTagBFP2-Farnesyl-5 were generated by M.W.D.

Amplification:

Article Title: UDP-N-acetylglucosamine Transporter (SLC35A3) Regulates Biosynthesis of Highly Branched N-Glycans and Keratan Sulfate
Article Snippet: .. ORF of human UDP-xylose/ N -acetylglucosamine transporter (SLC35B4; ) with BglII and BamHI restriction sites at both ends was amplified using cDNA synthesized from total RNA isolated from HeLa cells and subsequently cloned into pTagGFP2-C vector (Evrogen). .. All ligations were performed using a rapid DNA ligation kit (Fermentas).

Article Title: Actin Depletion Initiates Events Leading to Granule Secretion at the Immunological Synapse
Article Snippet: .. For CD3ζ-EGFP, full-length murine CD247 amplified by PCR from OT-I CTL cDNA was cloned into the XhoI and SalI sites of pEGFP-C1 (Clontech); for PACT-TagBFP, PACT ( ) was cloned into the BglII and XbaI sites of pTagBFP-C (Evrogen); for EGFP-MAPTau, MAPTau was subcloned into the XhoI and BamHI sites of pEGFP-N1; and for TagRFP-MEM-pMig, TagRFP was substituted in EYFP-MEM (Clontech) and Farnesyl-TagBFP2-pMig, which were subcloned into a pMig-R1 retroviral vector. .. Lifeact-mApple, Lifeact-mEmerald, mTagBFP2-MAPTau-N10, and mTagBFP2-Farnesyl-5 were generated by M.W.D.

Synthesized:

Article Title: UDP-N-acetylglucosamine Transporter (SLC35A3) Regulates Biosynthesis of Highly Branched N-Glycans and Keratan Sulfate
Article Snippet: .. ORF of human UDP-xylose/ N -acetylglucosamine transporter (SLC35B4; ) with BglII and BamHI restriction sites at both ends was amplified using cDNA synthesized from total RNA isolated from HeLa cells and subsequently cloned into pTagGFP2-C vector (Evrogen). .. All ligations were performed using a rapid DNA ligation kit (Fermentas).

Mutagenesis:

Article Title: Trafficking of Hepatitis C Virus Core Protein during Virus Particle Assembly
Article Snippet: .. To construct a fluorescent protein-tagged tubulin vector, the tubulin gene was excised from pmTFP-tubulin (Allele Biotechnology, San Diego CA) by using BglII and BamHI and subcloned into the BglII site of pTagRFP-C (Evrogen, Moscow Russia) previously modified to contain a S158T mutation that enhances photostability . ..

Isolation:

Article Title: UDP-N-acetylglucosamine Transporter (SLC35A3) Regulates Biosynthesis of Highly Branched N-Glycans and Keratan Sulfate
Article Snippet: .. ORF of human UDP-xylose/ N -acetylglucosamine transporter (SLC35B4; ) with BglII and BamHI restriction sites at both ends was amplified using cDNA synthesized from total RNA isolated from HeLa cells and subsequently cloned into pTagGFP2-C vector (Evrogen). .. All ligations were performed using a rapid DNA ligation kit (Fermentas).

Construct:

Article Title: Trafficking of Hepatitis C Virus Core Protein during Virus Particle Assembly
Article Snippet: .. To construct a fluorescent protein-tagged tubulin vector, the tubulin gene was excised from pmTFP-tubulin (Allele Biotechnology, San Diego CA) by using BglII and BamHI and subcloned into the BglII site of pTagRFP-C (Evrogen, Moscow Russia) previously modified to contain a S158T mutation that enhances photostability . ..

CTL Assay:

Article Title: Actin Depletion Initiates Events Leading to Granule Secretion at the Immunological Synapse
Article Snippet: .. For CD3ζ-EGFP, full-length murine CD247 amplified by PCR from OT-I CTL cDNA was cloned into the XhoI and SalI sites of pEGFP-C1 (Clontech); for PACT-TagBFP, PACT ( ) was cloned into the BglII and XbaI sites of pTagBFP-C (Evrogen); for EGFP-MAPTau, MAPTau was subcloned into the XhoI and BamHI sites of pEGFP-N1; and for TagRFP-MEM-pMig, TagRFP was substituted in EYFP-MEM (Clontech) and Farnesyl-TagBFP2-pMig, which were subcloned into a pMig-R1 retroviral vector. .. Lifeact-mApple, Lifeact-mEmerald, mTagBFP2-MAPTau-N10, and mTagBFP2-Farnesyl-5 were generated by M.W.D.

Polymerase Chain Reaction:

Article Title: Actin Depletion Initiates Events Leading to Granule Secretion at the Immunological Synapse
Article Snippet: .. For CD3ζ-EGFP, full-length murine CD247 amplified by PCR from OT-I CTL cDNA was cloned into the XhoI and SalI sites of pEGFP-C1 (Clontech); for PACT-TagBFP, PACT ( ) was cloned into the BglII and XbaI sites of pTagBFP-C (Evrogen); for EGFP-MAPTau, MAPTau was subcloned into the XhoI and BamHI sites of pEGFP-N1; and for TagRFP-MEM-pMig, TagRFP was substituted in EYFP-MEM (Clontech) and Farnesyl-TagBFP2-pMig, which were subcloned into a pMig-R1 retroviral vector. .. Lifeact-mApple, Lifeact-mEmerald, mTagBFP2-MAPTau-N10, and mTagBFP2-Farnesyl-5 were generated by M.W.D.

Modification:

Article Title: Trafficking of Hepatitis C Virus Core Protein during Virus Particle Assembly
Article Snippet: .. To construct a fluorescent protein-tagged tubulin vector, the tubulin gene was excised from pmTFP-tubulin (Allele Biotechnology, San Diego CA) by using BglII and BamHI and subcloned into the BglII site of pTagRFP-C (Evrogen, Moscow Russia) previously modified to contain a S158T mutation that enhances photostability . ..

Plasmid Preparation:

Article Title: UDP-N-acetylglucosamine Transporter (SLC35A3) Regulates Biosynthesis of Highly Branched N-Glycans and Keratan Sulfate
Article Snippet: .. ORF of human UDP-xylose/ N -acetylglucosamine transporter (SLC35B4; ) with BglII and BamHI restriction sites at both ends was amplified using cDNA synthesized from total RNA isolated from HeLa cells and subsequently cloned into pTagGFP2-C vector (Evrogen). .. All ligations were performed using a rapid DNA ligation kit (Fermentas).

Article Title: Actin Depletion Initiates Events Leading to Granule Secretion at the Immunological Synapse
Article Snippet: .. For CD3ζ-EGFP, full-length murine CD247 amplified by PCR from OT-I CTL cDNA was cloned into the XhoI and SalI sites of pEGFP-C1 (Clontech); for PACT-TagBFP, PACT ( ) was cloned into the BglII and XbaI sites of pTagBFP-C (Evrogen); for EGFP-MAPTau, MAPTau was subcloned into the XhoI and BamHI sites of pEGFP-N1; and for TagRFP-MEM-pMig, TagRFP was substituted in EYFP-MEM (Clontech) and Farnesyl-TagBFP2-pMig, which were subcloned into a pMig-R1 retroviral vector. .. Lifeact-mApple, Lifeact-mEmerald, mTagBFP2-MAPTau-N10, and mTagBFP2-Farnesyl-5 were generated by M.W.D.

Article Title: Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization
Article Snippet: .. Plasmid pL7-mGFP-actin was created by inserting a BglII, BamHI fragment encoding human β-actin from pPA-TagRFP-actin (#FP813, Evrogen, RU) in proper orientation into the BglII-site of pL7-mGFP. .. To generate plasmid pL7-FRED-WAVE1ΔVCA encoding WAVE1 lacking the VCA domain and fused at its N-terminus to FusionRed, a 1503 bp DNA fragment containing mouse Wasf1 sequence (XM_006512932.2, nucleotides 468 to 1955) flanked by a BglII site at the 5′-end and a stop codon and SalI site at the 3′-end (i.e., 5′-agatct-CCGTTGGTG…AGTGACGCA-tgagtcgac, Wasf1 sequence in upper case letters) was synthesized (Eurofins Genomics GmbH), digested with BglII and SalI, and ligated with vector backbone obtained by BglII, SalI digest of pL7-FusionRed.

Article Title: Trafficking of Hepatitis C Virus Core Protein during Virus Particle Assembly
Article Snippet: .. To construct a fluorescent protein-tagged tubulin vector, the tubulin gene was excised from pmTFP-tubulin (Allele Biotechnology, San Diego CA) by using BglII and BamHI and subcloned into the BglII site of pTagRFP-C (Evrogen, Moscow Russia) previously modified to contain a S158T mutation that enhances photostability . ..

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  • 93
    Evrogen bglii
    Bglii, supplied by Evrogen, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii/product/Evrogen
    Average 93 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    bglii - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    85
    Evrogen bglii hindiii restriction sites
    Bglii Hindiii Restriction Sites, supplied by Evrogen, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii hindiii restriction sites/product/Evrogen
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bglii hindiii restriction sites - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    91
    Evrogen bglii site
    Bglii Site, supplied by Evrogen, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii site/product/Evrogen
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bglii site - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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