bglii xbai  (New England Biolabs)


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    Structured Review

    New England Biolabs bglii xbai
    Bglii Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii xbai/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bglii xbai - by Bioz Stars, 2020-04
    86/100 stars

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    Electroporation:

    Article Title: Clostridiolysin S, a Post-translationally Modified Biotoxin from Clostridium botulinum *
    Article Snippet: Sequential restriction enzyme digests, BglII/XbaI for the pDCerm plasmid ( ) and BamHI/XbaI for the closA–D amplicons followed (New England Biolabs). .. The resulting ligation product was then transformed into E. coli MC1061 competent cells via electroporation and then plated onto LAerm500 plates.

    Amplification:

    Article Title: Clostridiolysin S, a Post-translationally Modified Biotoxin from Clostridium botulinum *
    Article Snippet: Each PCR amplicon was verified on a 1.0% gel, and the positively identified closA–D amplicons were then excised and purified using a Promega PCR purification wizard kit. .. Sequential restriction enzyme digests, BglII/XbaI for the pDCerm plasmid ( ) and BamHI/XbaI for the closA–D amplicons followed (New England Biolabs).

    Agarose Gel Electrophoresis:

    Article Title: Clostridiolysin S, a Post-translationally Modified Biotoxin from Clostridium botulinum *
    Article Snippet: Sequential restriction enzyme digests, BglII/XbaI for the pDCerm plasmid ( ) and BamHI/XbaI for the closA–D amplicons followed (New England Biolabs). .. Restriction enzyme products were separated on a 1.0% agarose gel, excised, and purified by the use of a Promega gel extraction wizard kit.

    Ligation:

    Article Title: Clostridiolysin S, a Post-translationally Modified Biotoxin from Clostridium botulinum *
    Article Snippet: Sequential restriction enzyme digests, BglII/XbaI for the pDCerm plasmid ( ) and BamHI/XbaI for the closA–D amplicons followed (New England Biolabs). .. Ligation was performed by the use of a 3:1 insert to vector ratio with a ligation kit (Roche Applied Science).

    Introduce:

    Article Title: Clostridiolysin S, a Post-translationally Modified Biotoxin from Clostridium botulinum *
    Article Snippet: To introduce the individual closA–D genes in trans to the corresponding sagA–D allelic exchange mutants, closA–D were individually PCR-amplified with XbaI/BamHI flanking sequences from pGS-21a closA–D gene containing vectors (GenScript). .. Sequential restriction enzyme digests, BglII/XbaI for the pDCerm plasmid ( ) and BamHI/XbaI for the closA–D amplicons followed (New England Biolabs).

    Purification:

    Article Title: Clostridiolysin S, a Post-translationally Modified Biotoxin from Clostridium botulinum *
    Article Snippet: Each PCR amplicon was verified on a 1.0% gel, and the positively identified closA–D amplicons were then excised and purified using a Promega PCR purification wizard kit. .. Sequential restriction enzyme digests, BglII/XbaI for the pDCerm plasmid ( ) and BamHI/XbaI for the closA–D amplicons followed (New England Biolabs).

    Polymerase Chain Reaction:

    Article Title: Clostridiolysin S, a Post-translationally Modified Biotoxin from Clostridium botulinum *
    Article Snippet: Each PCR amplicon was verified on a 1.0% gel, and the positively identified closA–D amplicons were then excised and purified using a Promega PCR purification wizard kit. .. Sequential restriction enzyme digests, BglII/XbaI for the pDCerm plasmid ( ) and BamHI/XbaI for the closA–D amplicons followed (New England Biolabs).

    Gel Extraction:

    Article Title: Clostridiolysin S, a Post-translationally Modified Biotoxin from Clostridium botulinum *
    Article Snippet: Sequential restriction enzyme digests, BglII/XbaI for the pDCerm plasmid ( ) and BamHI/XbaI for the closA–D amplicons followed (New England Biolabs). .. Restriction enzyme products were separated on a 1.0% agarose gel, excised, and purified by the use of a Promega gel extraction wizard kit.

    Transformation Assay:

    Article Title: Clostridiolysin S, a Post-translationally Modified Biotoxin from Clostridium botulinum *
    Article Snippet: Sequential restriction enzyme digests, BglII/XbaI for the pDCerm plasmid ( ) and BamHI/XbaI for the closA–D amplicons followed (New England Biolabs). .. The resulting ligation product was then transformed into E. coli MC1061 competent cells via electroporation and then plated onto LAerm500 plates.

    Plasmid Preparation:

    Article Title: Clostridiolysin S, a Post-translationally Modified Biotoxin from Clostridium botulinum *
    Article Snippet: .. Sequential restriction enzyme digests, BglII/XbaI for the pDCerm plasmid ( ) and BamHI/XbaI for the closA–D amplicons followed (New England Biolabs). .. Restriction enzyme products were separated on a 1.0% agarose gel, excised, and purified by the use of a Promega gel extraction wizard kit.

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    New England Biolabs bglii xbai restriction sites
    Bglii Xbai Restriction Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii xbai restriction sites/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bglii xbai restriction sites - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    86
    New England Biolabs bglii xbai
    Bglii Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii xbai/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bglii xbai - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

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