basic fibroblast growth factor bfgf  (Alomone Labs)


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    Alomone Labs basic fibroblast growth factor bfgf
    Basic Fibroblast Growth Factor Bfgf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bfgf  (Alomone Labs)


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    Alomone Labs bfgf
    Bfgf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bfgf  (Alomone Labs)


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    Alomone Labs bfgf
    Bfgf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bfgf  (Alomone Labs)


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    Alomone Labs bfgf
    Bfgf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bfgf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bfgf  (Alomone Labs)


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    Bfgf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs bfgf
    Bfgf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs bfgf
    Bfgf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    basic fibroblast growth factor bfgf  (Alomone Labs)


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    Alomone Labs basic fibroblast growth factor bfgf
    Basic Fibroblast Growth Factor Bfgf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bfgf  (Alomone Labs)


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    Alomone Labs bfgf
    Neurog2 confers ERK5-dependent pro-neural activity in SGZ-derived aNPCs. SGZ-derived aNPCs were infected with control retroviral and lentiviral vectors expressing eGFP only (A–D), lentiviral Neurog2 only (E–H), and lentiviral Neurog2 together with control retroviral shNS (J–M) or shERK5 (N–Q). One day after virus infection, cells were washed and then maintained in <t>regular</t> <t>EGF-</t> and <t>bFGF-containing</t> medium for 5 days before co-immunostaining for GFP (green) and various cellular markers (red) including β-III tubulin or Sox2 as indicated. The percentage of GFP+ cells that also express β-III tubulin or Sox2 were quantified (I and R). Scale bar in A represents 25 μm and applies to all images. Arrowheads point to GFP+ co-labeled cells, whereas arrows point to GFP+ cells that are not co-labeled with cell-type specific markers.
    Bfgf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Inducible and Conditional Deletion of Extracellular Signal-regulated Kinase 5 Disrupts Adult Hippocampal Neurogenesis"

    Article Title: Inducible and Conditional Deletion of Extracellular Signal-regulated Kinase 5 Disrupts Adult Hippocampal Neurogenesis

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.344762

    Neurog2 confers ERK5-dependent pro-neural activity in SGZ-derived aNPCs. SGZ-derived aNPCs were infected with control retroviral and lentiviral vectors expressing eGFP only (A–D), lentiviral Neurog2 only (E–H), and lentiviral Neurog2 together with control retroviral shNS (J–M) or shERK5 (N–Q). One day after virus infection, cells were washed and then maintained in regular EGF- and bFGF-containing medium for 5 days before co-immunostaining for GFP (green) and various cellular markers (red) including β-III tubulin or Sox2 as indicated. The percentage of GFP+ cells that also express β-III tubulin or Sox2 were quantified (I and R). Scale bar in A represents 25 μm and applies to all images. Arrowheads point to GFP+ co-labeled cells, whereas arrows point to GFP+ cells that are not co-labeled with cell-type specific markers.
    Figure Legend Snippet: Neurog2 confers ERK5-dependent pro-neural activity in SGZ-derived aNPCs. SGZ-derived aNPCs were infected with control retroviral and lentiviral vectors expressing eGFP only (A–D), lentiviral Neurog2 only (E–H), and lentiviral Neurog2 together with control retroviral shNS (J–M) or shERK5 (N–Q). One day after virus infection, cells were washed and then maintained in regular EGF- and bFGF-containing medium for 5 days before co-immunostaining for GFP (green) and various cellular markers (red) including β-III tubulin or Sox2 as indicated. The percentage of GFP+ cells that also express β-III tubulin or Sox2 were quantified (I and R). Scale bar in A represents 25 μm and applies to all images. Arrowheads point to GFP+ co-labeled cells, whereas arrows point to GFP+ cells that are not co-labeled with cell-type specific markers.

    Techniques Used: Activity Assay, Derivative Assay, Infection, Expressing, Immunostaining, Labeling

    ERK5 signaling is necessary for promoting neurogenesis of SGZ-derived aNPCs in culture. aNPCs were infected with nonspecific shRNA control retroviral vector (shNS) or shRNA to ERK5 retrovirus as indicated. Both retroviral vectors encode eGFP marker protein under a bicistronic promoter. One day after virus infection, cells were washed and then incubated in culture medium free of EGF and bFGF for 5 days to allow spontaneous differentiation. Cells were then fixed for immunocytochemistry and co-stained for GFP to identify virus-infected cells (green) and various cell-type specific markers (red). A–D, co-staining of GFP with β-III tubulin. E, quantification of the percentage of GFP+ cells that are also β-III tubulin+. F–I, immunostaining of GFP+-infected cells and Nestin+ neural stem cells. J–M, immunostaining of GFP+-infected cells and PCNA+ proliferating cells. N, quantification of the percentage of GFP+ cells that also express Sox2, Nestin, or PCNA. Scale bar in A represents 25 μm and applies to all images. Arrowheads point to GFP+ cells co-labeled with various cell markers, whereas arrows point to GFP+ cells that are marker negative.
    Figure Legend Snippet: ERK5 signaling is necessary for promoting neurogenesis of SGZ-derived aNPCs in culture. aNPCs were infected with nonspecific shRNA control retroviral vector (shNS) or shRNA to ERK5 retrovirus as indicated. Both retroviral vectors encode eGFP marker protein under a bicistronic promoter. One day after virus infection, cells were washed and then incubated in culture medium free of EGF and bFGF for 5 days to allow spontaneous differentiation. Cells were then fixed for immunocytochemistry and co-stained for GFP to identify virus-infected cells (green) and various cell-type specific markers (red). A–D, co-staining of GFP with β-III tubulin. E, quantification of the percentage of GFP+ cells that are also β-III tubulin+. F–I, immunostaining of GFP+-infected cells and Nestin+ neural stem cells. J–M, immunostaining of GFP+-infected cells and PCNA+ proliferating cells. N, quantification of the percentage of GFP+ cells that also express Sox2, Nestin, or PCNA. Scale bar in A represents 25 μm and applies to all images. Arrowheads point to GFP+ cells co-labeled with various cell markers, whereas arrows point to GFP+ cells that are marker negative.

    Techniques Used: Derivative Assay, Infection, shRNA, Plasmid Preparation, Marker, Incubation, Immunocytochemistry, Staining, Immunostaining, Labeling

    Activation of endogenous ERK5 signaling is sufficient to promote neurogenesis of dentate gyrus-derived aNPCs in culture. A, anti-ERK5 and MEK5 Western analysis of NIH-3T3 cells infected with retroviruses expressing caMEK5 and/or wild-type (wt−) ERK5. Cells infected with GFP retrovirus (vector only) were used as controls. β-Actin was used as a loading control. Expression of caMEK5 caused reduced electrophoretic mobility of endogenous ERK5 (lanes 1 and 2) as well as the co-infected wtERK5 (lanes 3 and 4), indicative of ERK5 phosphorylation (p-ERK5) and activation. These data ascertain the constitutive active nature of the caMEK5 virus. B–O, aNPCs were infected with control retroviral vector expressing eGFP only or expressing caMEK5-IRES-eGFP. One day after virus infection, cells were washed and then maintained in regular EGF- and bFGF-containing medium for 5 days before co-immunostaining for GFP (green) and various cellular markers (red) including β-III tubulin (B–E), Nestin (G–J), and PCNA (K–N). The percentage of GFP+ cells that also express β-III tubulin (F), Sox2, Nestin, or PCNA (O) was quantified. Scale bar in B represents 25 μm and applies to all images. Arrowheads point to GFP+ cells co-labeled with various cell markers, whereas arrows point to GFP+ cells that are marker negative.
    Figure Legend Snippet: Activation of endogenous ERK5 signaling is sufficient to promote neurogenesis of dentate gyrus-derived aNPCs in culture. A, anti-ERK5 and MEK5 Western analysis of NIH-3T3 cells infected with retroviruses expressing caMEK5 and/or wild-type (wt−) ERK5. Cells infected with GFP retrovirus (vector only) were used as controls. β-Actin was used as a loading control. Expression of caMEK5 caused reduced electrophoretic mobility of endogenous ERK5 (lanes 1 and 2) as well as the co-infected wtERK5 (lanes 3 and 4), indicative of ERK5 phosphorylation (p-ERK5) and activation. These data ascertain the constitutive active nature of the caMEK5 virus. B–O, aNPCs were infected with control retroviral vector expressing eGFP only or expressing caMEK5-IRES-eGFP. One day after virus infection, cells were washed and then maintained in regular EGF- and bFGF-containing medium for 5 days before co-immunostaining for GFP (green) and various cellular markers (red) including β-III tubulin (B–E), Nestin (G–J), and PCNA (K–N). The percentage of GFP+ cells that also express β-III tubulin (F), Sox2, Nestin, or PCNA (O) was quantified. Scale bar in B represents 25 μm and applies to all images. Arrowheads point to GFP+ cells co-labeled with various cell markers, whereas arrows point to GFP+ cells that are marker negative.

    Techniques Used: Activation Assay, Derivative Assay, Western Blot, Infection, Expressing, Plasmid Preparation, Immunostaining, Labeling, Marker

    NT3 activates ERK5 and stimulates neuronal differentiation of SGZ-derived aNPCs through ERK5. A, BDNF and NT3 induce ERK5 phosphorylation, indicative of ERK5 activation. SGZ-derived aNPCs were treated with vehicle control (NS, lane 1), 50 ng/ml of BDNF (lanes 2–5), or 100 ng/ml of NT3 (lanes 6–9) for the indicated times. Total ERK5 (T-ERK5) was used as a loading control. B, NT3 stimulates neuronal differentiation of SGZ-derived aNPCs through a process that requires ERK5. SGZ-derived aNPCs were infected with retroviruses encoding a nonspecific control (shNS) or shERK5. Cells were then incubated in EGF/bFGF-free medium and treated with NT3 (100 ng/ml) for 5 days to induce neuronal differentiation. Cells treated with BSA (1 μg/ml) were used as a control.
    Figure Legend Snippet: NT3 activates ERK5 and stimulates neuronal differentiation of SGZ-derived aNPCs through ERK5. A, BDNF and NT3 induce ERK5 phosphorylation, indicative of ERK5 activation. SGZ-derived aNPCs were treated with vehicle control (NS, lane 1), 50 ng/ml of BDNF (lanes 2–5), or 100 ng/ml of NT3 (lanes 6–9) for the indicated times. Total ERK5 (T-ERK5) was used as a loading control. B, NT3 stimulates neuronal differentiation of SGZ-derived aNPCs through a process that requires ERK5. SGZ-derived aNPCs were infected with retroviruses encoding a nonspecific control (shNS) or shERK5. Cells were then incubated in EGF/bFGF-free medium and treated with NT3 (100 ng/ml) for 5 days to induce neuronal differentiation. Cells treated with BSA (1 μg/ml) were used as a control.

    Techniques Used: Derivative Assay, Activation Assay, Infection, Incubation

    bfgf  (Alomone Labs)


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    Alomone Labs bfgf
    Neurog2 confers ERK5-dependent pro-neural activity in SGZ-derived aNPCs. SGZ-derived aNPCs were infected with control retroviral and lentiviral vectors expressing eGFP only (A–D), lentiviral Neurog2 only (E–H), and lentiviral Neurog2 together with control retroviral shNS (J–M) or shERK5 (N–Q). One day after virus infection, cells were washed and then maintained in <t>regular</t> <t>EGF-</t> and <t>bFGF-containing</t> medium for 5 days before co-immunostaining for GFP (green) and various cellular markers (red) including β-III tubulin or Sox2 as indicated. The percentage of GFP+ cells that also express β-III tubulin or Sox2 were quantified (I and R). Scale bar in A represents 25 μm and applies to all images. Arrowheads point to GFP+ co-labeled cells, whereas arrows point to GFP+ cells that are not co-labeled with cell-type specific markers.
    Bfgf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bfgf/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bfgf - by Bioz Stars, 2023-01
    86/100 stars

    Images

    1) Product Images from "Inducible and Conditional Deletion of Extracellular Signal-regulated Kinase 5 Disrupts Adult Hippocampal Neurogenesis * "

    Article Title: Inducible and Conditional Deletion of Extracellular Signal-regulated Kinase 5 Disrupts Adult Hippocampal Neurogenesis *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.344762

    Neurog2 confers ERK5-dependent pro-neural activity in SGZ-derived aNPCs. SGZ-derived aNPCs were infected with control retroviral and lentiviral vectors expressing eGFP only (A–D), lentiviral Neurog2 only (E–H), and lentiviral Neurog2 together with control retroviral shNS (J–M) or shERK5 (N–Q). One day after virus infection, cells were washed and then maintained in regular EGF- and bFGF-containing medium for 5 days before co-immunostaining for GFP (green) and various cellular markers (red) including β-III tubulin or Sox2 as indicated. The percentage of GFP+ cells that also express β-III tubulin or Sox2 were quantified (I and R). Scale bar in A represents 25 μm and applies to all images. Arrowheads point to GFP+ co-labeled cells, whereas arrows point to GFP+ cells that are not co-labeled with cell-type specific markers.
    Figure Legend Snippet: Neurog2 confers ERK5-dependent pro-neural activity in SGZ-derived aNPCs. SGZ-derived aNPCs were infected with control retroviral and lentiviral vectors expressing eGFP only (A–D), lentiviral Neurog2 only (E–H), and lentiviral Neurog2 together with control retroviral shNS (J–M) or shERK5 (N–Q). One day after virus infection, cells were washed and then maintained in regular EGF- and bFGF-containing medium for 5 days before co-immunostaining for GFP (green) and various cellular markers (red) including β-III tubulin or Sox2 as indicated. The percentage of GFP+ cells that also express β-III tubulin or Sox2 were quantified (I and R). Scale bar in A represents 25 μm and applies to all images. Arrowheads point to GFP+ co-labeled cells, whereas arrows point to GFP+ cells that are not co-labeled with cell-type specific markers.

    Techniques Used: Activity Assay, Derivative Assay, Infection, Expressing, Immunostaining, Labeling

    ERK5 signaling is necessary for promoting neurogenesis of SGZ-derived aNPCs in culture. aNPCs were infected with nonspecific shRNA control retroviral vector (shNS) or shRNA to ERK5 retrovirus as indicated. Both retroviral vectors encode eGFP marker protein under a bicistronic promoter. One day after virus infection, cells were washed and then incubated in culture medium free of EGF and bFGF for 5 days to allow spontaneous differentiation. Cells were then fixed for immunocytochemistry and co-stained for GFP to identify virus-infected cells (green) and various cell-type specific markers (red). A–D, co-staining of GFP with β-III tubulin. E, quantification of the percentage of GFP+ cells that are also β-III tubulin+. F–I, immunostaining of GFP+-infected cells and Nestin+ neural stem cells. J–M, immunostaining of GFP+-infected cells and PCNA+ proliferating cells. N, quantification of the percentage of GFP+ cells that also express Sox2, Nestin, or PCNA. Scale bar in A represents 25 μm and applies to all images. Arrowheads point to GFP+ cells co-labeled with various cell markers, whereas arrows point to GFP+ cells that are marker negative.
    Figure Legend Snippet: ERK5 signaling is necessary for promoting neurogenesis of SGZ-derived aNPCs in culture. aNPCs were infected with nonspecific shRNA control retroviral vector (shNS) or shRNA to ERK5 retrovirus as indicated. Both retroviral vectors encode eGFP marker protein under a bicistronic promoter. One day after virus infection, cells were washed and then incubated in culture medium free of EGF and bFGF for 5 days to allow spontaneous differentiation. Cells were then fixed for immunocytochemistry and co-stained for GFP to identify virus-infected cells (green) and various cell-type specific markers (red). A–D, co-staining of GFP with β-III tubulin. E, quantification of the percentage of GFP+ cells that are also β-III tubulin+. F–I, immunostaining of GFP+-infected cells and Nestin+ neural stem cells. J–M, immunostaining of GFP+-infected cells and PCNA+ proliferating cells. N, quantification of the percentage of GFP+ cells that also express Sox2, Nestin, or PCNA. Scale bar in A represents 25 μm and applies to all images. Arrowheads point to GFP+ cells co-labeled with various cell markers, whereas arrows point to GFP+ cells that are marker negative.

    Techniques Used: Derivative Assay, Infection, shRNA, Plasmid Preparation, Marker, Incubation, Immunocytochemistry, Staining, Immunostaining, Labeling

    Activation of endogenous ERK5 signaling is sufficient to promote neurogenesis of dentate gyrus-derived aNPCs in culture. A, anti-ERK5 and MEK5 Western analysis of NIH-3T3 cells infected with retroviruses expressing caMEK5 and/or wild-type (wt−) ERK5. Cells infected with GFP retrovirus (vector only) were used as controls. β-Actin was used as a loading control. Expression of caMEK5 caused reduced electrophoretic mobility of endogenous ERK5 (lanes 1 and 2) as well as the co-infected wtERK5 (lanes 3 and 4), indicative of ERK5 phosphorylation (p-ERK5) and activation. These data ascertain the constitutive active nature of the caMEK5 virus. B–O, aNPCs were infected with control retroviral vector expressing eGFP only or expressing caMEK5-IRES-eGFP. One day after virus infection, cells were washed and then maintained in regular EGF- and bFGF-containing medium for 5 days before co-immunostaining for GFP (green) and various cellular markers (red) including β-III tubulin (B–E), Nestin (G–J), and PCNA (K–N). The percentage of GFP+ cells that also express β-III tubulin (F), Sox2, Nestin, or PCNA (O) was quantified. Scale bar in B represents 25 μm and applies to all images. Arrowheads point to GFP+ cells co-labeled with various cell markers, whereas arrows point to GFP+ cells that are marker negative.
    Figure Legend Snippet: Activation of endogenous ERK5 signaling is sufficient to promote neurogenesis of dentate gyrus-derived aNPCs in culture. A, anti-ERK5 and MEK5 Western analysis of NIH-3T3 cells infected with retroviruses expressing caMEK5 and/or wild-type (wt−) ERK5. Cells infected with GFP retrovirus (vector only) were used as controls. β-Actin was used as a loading control. Expression of caMEK5 caused reduced electrophoretic mobility of endogenous ERK5 (lanes 1 and 2) as well as the co-infected wtERK5 (lanes 3 and 4), indicative of ERK5 phosphorylation (p-ERK5) and activation. These data ascertain the constitutive active nature of the caMEK5 virus. B–O, aNPCs were infected with control retroviral vector expressing eGFP only or expressing caMEK5-IRES-eGFP. One day after virus infection, cells were washed and then maintained in regular EGF- and bFGF-containing medium for 5 days before co-immunostaining for GFP (green) and various cellular markers (red) including β-III tubulin (B–E), Nestin (G–J), and PCNA (K–N). The percentage of GFP+ cells that also express β-III tubulin (F), Sox2, Nestin, or PCNA (O) was quantified. Scale bar in B represents 25 μm and applies to all images. Arrowheads point to GFP+ cells co-labeled with various cell markers, whereas arrows point to GFP+ cells that are marker negative.

    Techniques Used: Activation Assay, Derivative Assay, Western Blot, Infection, Expressing, Plasmid Preparation, Immunostaining, Labeling, Marker

    NT3 activates ERK5 and stimulates neuronal differentiation of SGZ-derived aNPCs through ERK5. A, BDNF and NT3 induce ERK5 phosphorylation, indicative of ERK5 activation. SGZ-derived aNPCs were treated with vehicle control (NS, lane 1), 50 ng/ml of BDNF (lanes 2–5), or 100 ng/ml of NT3 (lanes 6–9) for the indicated times. Total ERK5 (T-ERK5) was used as a loading control. B, NT3 stimulates neuronal differentiation of SGZ-derived aNPCs through a process that requires ERK5. SGZ-derived aNPCs were infected with retroviruses encoding a nonspecific control (shNS) or shERK5. Cells were then incubated in EGF/bFGF-free medium and treated with NT3 (100 ng/ml) for 5 days to induce neuronal differentiation. Cells treated with BSA (1 μg/ml) were used as a control.
    Figure Legend Snippet: NT3 activates ERK5 and stimulates neuronal differentiation of SGZ-derived aNPCs through ERK5. A, BDNF and NT3 induce ERK5 phosphorylation, indicative of ERK5 activation. SGZ-derived aNPCs were treated with vehicle control (NS, lane 1), 50 ng/ml of BDNF (lanes 2–5), or 100 ng/ml of NT3 (lanes 6–9) for the indicated times. Total ERK5 (T-ERK5) was used as a loading control. B, NT3 stimulates neuronal differentiation of SGZ-derived aNPCs through a process that requires ERK5. SGZ-derived aNPCs were infected with retroviruses encoding a nonspecific control (shNS) or shERK5. Cells were then incubated in EGF/bFGF-free medium and treated with NT3 (100 ng/ml) for 5 days to induce neuronal differentiation. Cells treated with BSA (1 μg/ml) were used as a control.

    Techniques Used: Derivative Assay, Activation Assay, Infection, Incubation

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    Alomone Labs basic fibroblast growth factor bfgf
    Basic Fibroblast Growth Factor Bfgf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    basic fibroblast growth factor bfgf - by Bioz Stars, 2023-01
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    Alomone Labs bfgf
    Bfgf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bfgf/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bfgf - by Bioz Stars, 2023-01
    86/100 stars
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