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Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on <t>BEI</t> Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) <t>MARV</t> plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.
Bei, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies

Journal: Viruses

doi: 10.3390/v4123511

Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on BEI Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) MARV plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.
Figure Legend Snippet: Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on BEI Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) MARV plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.

Techniques Used: Produced

( A ) EBOV titers in ATCC or BEI Vero E6 cells of various passage ages. This experiment was performed by three independent investigators, four replicates per cell type. The combined data are presented, where each bar represents 12 replicates. * indicates a significant difference in value between BEI passage 40 and passage 27 ( p = 0.0003), ** a difference between ATCC passage 54 and 28 ( p = 0.007), and *** a difference between BEI passage 40 and ATCC passage 28 ( p = 0.000002). For these experiments, p value cutoff was ≤0.008). ( B ) Analysis of EBOV titer changes in cells of various passages. The arrows point out data from passages plotted in (A).
Figure Legend Snippet: ( A ) EBOV titers in ATCC or BEI Vero E6 cells of various passage ages. This experiment was performed by three independent investigators, four replicates per cell type. The combined data are presented, where each bar represents 12 replicates. * indicates a significant difference in value between BEI passage 40 and passage 27 ( p = 0.0003), ** a difference between ATCC passage 54 and 28 ( p = 0.007), and *** a difference between BEI passage 40 and ATCC passage 28 ( p = 0.000002). For these experiments, p value cutoff was ≤0.008). ( B ) Analysis of EBOV titer changes in cells of various passages. The arrows point out data from passages plotted in (A).

Techniques Used:

Related Articles

Selection:

Article Title: Evaluation of PCR Systems for Field Screening of Bacillus anthracis
Article Snippet: The AOAC SMPR 2010.003 was used to guide the selection of inclusivity and exclusivity Bacillus strains for DNA-based testing. (See and and for further information.) .. We obtained DNA from BEI Resources, and for those DNA strains not available from BEI, we obtained cultures from the American Type Culture Collection (ATCC, Rockville, MD) and from Dr. Karen Hill of Los Alamos National Laboratory.

Plaque Assay:

Article Title: Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies
Article Snippet: At the completion of EBOV experiments, Vero E6 cells from ATCC and BEI were evaluated in the MARV plaque assay. .. MARV plaque quality on BEI and ATCC Vero E6 cells appears equivalent ( A).

Picogreen Assay:

Article Title: Evaluation of PCR Systems for Field Screening of Bacillus anthracis
Article Snippet: We obtained DNA from BEI Resources, and for those DNA strains not available from BEI, we obtained cultures from the American Type Culture Collection (ATCC, Rockville, MD) and from Dr. Karen Hill of Los Alamos National Laboratory. .. Exclusivity DNA that was prepared on-site was isolated from freshly cultured stocks using the Qiagen DNeasy Blood and Tissue kit (Valencia, CA) as per the manufacturer's protocol.

Mouse Assay:

Article Title: Prevention and treatment of Clostridium perfringens epsilon toxin intoxication in mice with a neutralizing monoclonal antibody (c4D7) produced in Nicotiana benthamiana
Article Snippet: Male and female Balb/C mice (17 to 21 g) housed in a temperature and light cycle controlled room were used. .. Trypsin-activated purified ETX was obtained from BEI (ATCC 3626).

Real-time Polymerase Chain Reaction:

Article Title: Evaluation of PCR Systems for Field Screening of Bacillus anthracis
Article Snippet: We obtained DNA from BEI Resources, and for those DNA strains not available from BEI, we obtained cultures from the American Type Culture Collection (ATCC, Rockville, MD) and from Dr. Karen Hill of Los Alamos National Laboratory. .. All DNA stocks were quantified using the Invitrogen Quant-iT™ PicoGreen® assay kit (Grand Island, NY), using either the lambda phage DNA provided in the kit or lambda phage DNA from New England Biolabs (Ipswitch, MA).

Modification:

Article Title: 699. Relationship Between Klebsiella pneumoniae Antimicrobial Resistance and Biofilm Formation
Article Snippet: Ctr., Rhode Island Hosp., BEI, and ATCC). .. Ctr., Rhode Island Hosp., BEI, and ATCC).

Inhibition:

Article Title: Evaluation of PCR Systems for Field Screening of Bacillus anthracis
Article Snippet: We obtained DNA from BEI Resources, and for those DNA strains not available from BEI, we obtained cultures from the American Type Culture Collection (ATCC, Rockville, MD) and from Dr. Karen Hill of Los Alamos National Laboratory. .. To confirm that the DNA stocks were free of PCR inhibitors, DNA from the inclusivity (2,000 GE/mL) and exclusivity (20,000 GE/mL) panel strains were analyzed by PCR in triplicate using an ABI 7500 Fast Real-Time PCR System (Life Technologies, Grand Island, NY), with primer and probe sets designed to detect the respective DNA.

T-Test:

Article Title: Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies
Article Snippet: MARV plaque quality on BEI and ATCC Vero E6 cells appears equivalent ( A). .. Therefore, head to head comparisons of virus titrations in Vero E6 cells from ATCC or BEI that had been in culture at USAMRIID for increasing lengths of time were performed three times in parallel with the same preparations of cells and with the same vial of seed stock virus ( A).

Polymerase Chain Reaction:

Article Title: Evaluation of PCR Systems for Field Screening of Bacillus anthracis
Article Snippet: We obtained DNA from BEI Resources, and for those DNA strains not available from BEI, we obtained cultures from the American Type Culture Collection (ATCC, Rockville, MD) and from Dr. Karen Hill of Los Alamos National Laboratory. .. We obtained DNA from BEI Resources, and for those DNA strains not available from BEI, we obtained cultures from the American Type Culture Collection (ATCC, Rockville, MD) and from Dr. Karen Hill of Los Alamos National Laboratory.

Injection:

Article Title: Prevention and treatment of Clostridium perfringens epsilon toxin intoxication in mice with a neutralizing monoclonal antibody (c4D7) produced in Nicotiana benthamiana
Article Snippet: Trypsin-activated purified ETX was obtained from BEI (ATCC 3626). .. Trypsin-activated purified ETX was obtained from BEI (ATCC 3626).

Purification:

Article Title: Prevention and treatment of Clostridium perfringens epsilon toxin intoxication in mice with a neutralizing monoclonal antibody (c4D7) produced in Nicotiana benthamiana
Article Snippet: Total injection volume (iv or ip) was always 0.5 ml. .. Trypsin-activated purified ETX was obtained from BEI (ATCC 3626). .. This toxin preparation was found to be > 95% pure.

Article Title: Evaluation of PCR Systems for Field Screening of Bacillus anthracis
Article Snippet: We obtained DNA from BEI Resources, and for those DNA strains not available from BEI, we obtained cultures from the American Type Culture Collection (ATCC, Rockville, MD) and from Dr. Karen Hill of Los Alamos National Laboratory. .. To confirm that the DNA stocks were free of PCR inhibitors, DNA from the inclusivity (2,000 GE/mL) and exclusivity (20,000 GE/mL) panel strains were analyzed by PCR in triplicate using an ABI 7500 Fast Real-Time PCR System (Life Technologies, Grand Island, NY), with primer and probe sets designed to detect the respective DNA.

Plasmid Preparation:

Article Title: Evaluation of PCR Systems for Field Screening of Bacillus anthracis
Article Snippet: The apparent PAK-1 strain obtained from CRP was found to be lacking the pXO2 plasmid; therefore, we obtained a PAK-1 strain from Midwest Research Institute (Kansas City, MO), which did test positive for pXO2 (see discussion below on DNA stock screening). .. We obtained DNA from BEI Resources, and for those DNA strains not available from BEI, we obtained cultures from the American Type Culture Collection (ATCC, Rockville, MD) and from Dr. Karen Hill of Los Alamos National Laboratory.

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    ATCC bei
    Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on <t>BEI</t> Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) <t>MARV</t> plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.
    Bei, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bei/product/ATCC
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bei - by Bioz Stars, 2019-10
    95/100 stars
      Buy from Supplier

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    Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on BEI Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) MARV plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.

    Journal: Viruses

    Article Title: Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies

    doi: 10.3390/v4123511

    Figure Lengend Snippet: Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on BEI Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) MARV plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.

    Article Snippet: MARV plaque quality on BEI and ATCC Vero E6 cells appears equivalent ( A).

    Techniques: Produced

    ( A ) EBOV titers in ATCC or BEI Vero E6 cells of various passage ages. This experiment was performed by three independent investigators, four replicates per cell type. The combined data are presented, where each bar represents 12 replicates. * indicates a significant difference in value between BEI passage 40 and passage 27 ( p = 0.0003), ** a difference between ATCC passage 54 and 28 ( p = 0.007), and *** a difference between BEI passage 40 and ATCC passage 28 ( p = 0.000002). For these experiments, p value cutoff was ≤0.008). ( B ) Analysis of EBOV titer changes in cells of various passages. The arrows point out data from passages plotted in (A).

    Journal: Viruses

    Article Title: Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies

    doi: 10.3390/v4123511

    Figure Lengend Snippet: ( A ) EBOV titers in ATCC or BEI Vero E6 cells of various passage ages. This experiment was performed by three independent investigators, four replicates per cell type. The combined data are presented, where each bar represents 12 replicates. * indicates a significant difference in value between BEI passage 40 and passage 27 ( p = 0.0003), ** a difference between ATCC passage 54 and 28 ( p = 0.007), and *** a difference between BEI passage 40 and ATCC passage 28 ( p = 0.000002). For these experiments, p value cutoff was ≤0.008). ( B ) Analysis of EBOV titer changes in cells of various passages. The arrows point out data from passages plotted in (A).

    Article Snippet: MARV plaque quality on BEI and ATCC Vero E6 cells appears equivalent ( A).

    Techniques: