bei  (ATCC)


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    ATCC bei
    Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on <t>BEI</t> Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) <t>MARV</t> plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.
    Bei, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 2 article reviews
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    bei - by Bioz Stars, 2020-05
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    Images

    1) Product Images from "Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies"

    Article Title: Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies

    Journal: Viruses

    doi: 10.3390/v4123511

    Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on BEI Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) MARV plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.
    Figure Legend Snippet: Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on BEI Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) MARV plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.

    Techniques Used: Produced

    ( A ) EBOV titers in ATCC or BEI Vero E6 cells of various passage ages. This experiment was performed by three independent investigators, four replicates per cell type. The combined data are presented, where each bar represents 12 replicates. * indicates a significant difference in value between BEI passage 40 and passage 27 ( p = 0.0003), ** a difference between ATCC passage 54 and 28 ( p = 0.007), and *** a difference between BEI passage 40 and ATCC passage 28 ( p = 0.000002). For these experiments, p value cutoff was ≤0.008). ( B ) Analysis of EBOV titer changes in cells of various passages. The arrows point out data from passages plotted in (A).
    Figure Legend Snippet: ( A ) EBOV titers in ATCC or BEI Vero E6 cells of various passage ages. This experiment was performed by three independent investigators, four replicates per cell type. The combined data are presented, where each bar represents 12 replicates. * indicates a significant difference in value between BEI passage 40 and passage 27 ( p = 0.0003), ** a difference between ATCC passage 54 and 28 ( p = 0.007), and *** a difference between BEI passage 40 and ATCC passage 28 ( p = 0.000002). For these experiments, p value cutoff was ≤0.008). ( B ) Analysis of EBOV titer changes in cells of various passages. The arrows point out data from passages plotted in (A).

    Techniques Used:

    2) Product Images from "Identification of Critical Amino Acids within the Nucleoprotein of Tacaribe Virus Important for Anti-interferon Activity *"

    Article Title: Identification of Critical Amino Acids within the Nucleoprotein of Tacaribe Virus Important for Anti-interferon Activity *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.444760

    Analysis of a TCRV NP with GPPT residues important for anti-IFN activity. A , alignment of protein sequences near the DLQL peptide stretch of the TCRV NP reference sequence ( REF ) to the sequences derived from BEI resources ( BEI ) and ATCC TCRV viral stocks. B–D , measurements of IFNβ reporter activity during expression of codon-optimized (reference sequence) TCRV NP with the DLQL sequence (TCRV NP) or containing GPPT in place of the DLQL residues (TNP-GPPT). Induction of the IFNβ promoter was performed by infection with SeV ( B ), transfection with 10 μg/ml low molecular weight poly(I:C) ( C ), or with 250 ng of purified TCRV RNA ( D ) following methods described under “Experimental Procedures.” E , increasing amounts of the vTNP, TNP-GPPT, and PICV NP ranging from 0.1 and 1 μg of plasmid DNA were transfected into 293T cells and subjected to the IFN activation assay explained under “Experimental Procedures.” The positive control was set as 100% activation, and the rest of the conditions were normalized to this value. Shown are the means of three experiments performed in triplicate (**, p
    Figure Legend Snippet: Analysis of a TCRV NP with GPPT residues important for anti-IFN activity. A , alignment of protein sequences near the DLQL peptide stretch of the TCRV NP reference sequence ( REF ) to the sequences derived from BEI resources ( BEI ) and ATCC TCRV viral stocks. B–D , measurements of IFNβ reporter activity during expression of codon-optimized (reference sequence) TCRV NP with the DLQL sequence (TCRV NP) or containing GPPT in place of the DLQL residues (TNP-GPPT). Induction of the IFNβ promoter was performed by infection with SeV ( B ), transfection with 10 μg/ml low molecular weight poly(I:C) ( C ), or with 250 ng of purified TCRV RNA ( D ) following methods described under “Experimental Procedures.” E , increasing amounts of the vTNP, TNP-GPPT, and PICV NP ranging from 0.1 and 1 μg of plasmid DNA were transfected into 293T cells and subjected to the IFN activation assay explained under “Experimental Procedures.” The positive control was set as 100% activation, and the rest of the conditions were normalized to this value. Shown are the means of three experiments performed in triplicate (**, p

    Techniques Used: Activity Assay, Sequencing, Derivative Assay, Expressing, Infection, Transfection, Molecular Weight, Purification, Plasmid Preparation, Activation Assay, Positive Control

    3) Product Images from "Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies"

    Article Title: Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies

    Journal: Viruses

    doi: 10.3390/v4123511

    Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on BEI Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) MARV plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.
    Figure Legend Snippet: Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on BEI Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) MARV plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.

    Techniques Used: Produced

    ( A ) EBOV titers in ATCC or BEI Vero E6 cells of various passage ages. This experiment was performed by three independent investigators, four replicates per cell type. The combined data are presented, where each bar represents 12 replicates. * indicates a significant difference in value between BEI passage 40 and passage 27 ( p = 0.0003), ** a difference between ATCC passage 54 and 28 ( p = 0.007), and *** a difference between BEI passage 40 and ATCC passage 28 ( p = 0.000002). For these experiments, p value cutoff was ≤0.008). ( B ) Analysis of EBOV titer changes in cells of various passages. The arrows point out data from passages plotted in (A).
    Figure Legend Snippet: ( A ) EBOV titers in ATCC or BEI Vero E6 cells of various passage ages. This experiment was performed by three independent investigators, four replicates per cell type. The combined data are presented, where each bar represents 12 replicates. * indicates a significant difference in value between BEI passage 40 and passage 27 ( p = 0.0003), ** a difference between ATCC passage 54 and 28 ( p = 0.007), and *** a difference between BEI passage 40 and ATCC passage 28 ( p = 0.000002). For these experiments, p value cutoff was ≤0.008). ( B ) Analysis of EBOV titer changes in cells of various passages. The arrows point out data from passages plotted in (A).

    Techniques Used:

    Related Articles

    Clone Assay:

    Article Title: Oligomerization of Clostridium perfringens Epsilon Toxin Is Dependent upon Caveolins 1 and 2
    Article Snippet: .. ε-toxin Expression and Purification The gene encoding epsilon prototoxin, etxB , from C. perfringens type B strain ATCC 3626 was cloned into plasmid pET22b (Novagen). .. This placed the etxB gene under the regulation of the bacteriophage T7 RNA polymerase and fused the C-terminal end of the prototoxin to a His6 affinity tag (to aid in purification of the protein).

    Construct:

    Article Title: Characterization of Virulence Plasmid Diversity among Clostridium perfringens Type B Isolates ▿
    Article Snippet: .. Based upon the C. perfringens type B strain ATCC 3626 sequence released by the J. Craig Venter Institute (JCVI), a series of primers were then constructed (Table ) to evaluate, by overlapping PCR, the potential linkage between tpeL and cpb in type B isolates. .. PCR conditions for these amplifications were as follows: 94°C for 3 min and 35 cycles of 94°C for 1 min, 55°C for 1 min, and 68°C for 1.5 min. PCR products were run on a 1% agarose gel and stained with ethidium bromide for visualization.

    Purification:

    Article Title: Oligomerization of Clostridium perfringens Epsilon Toxin Is Dependent upon Caveolins 1 and 2
    Article Snippet: .. ε-toxin Expression and Purification The gene encoding epsilon prototoxin, etxB , from C. perfringens type B strain ATCC 3626 was cloned into plasmid pET22b (Novagen). .. This placed the etxB gene under the regulation of the bacteriophage T7 RNA polymerase and fused the C-terminal end of the prototoxin to a His6 affinity tag (to aid in purification of the protein).

    Sequencing:

    Article Title: Characterization of Virulence Plasmid Diversity among Clostridium perfringens Type B Isolates ▿
    Article Snippet: .. Bioinformatic analyses of sequence data for type B strain ATCC 3626 released by JCVI identified two IS 1151- like sequences upstream of cpb . .. Therefore, a PCR analysis was performed using primers based upon the ATCC 3626 sequence to investigate whether IS 1151 -like sequences reside upstream of the cpb gene in other type B isolates.

    Article Title: Characterization of Virulence Plasmid Diversity among Clostridium perfringens Type B Isolates ▿
    Article Snippet: .. Therefore, a PCR analysis was performed using primers based upon the ATCC 3626 sequence to investigate whether IS 1151 -like sequences reside upstream of the cpb gene in other type B isolates. .. In this PCR, all four surveyed type B isolates (NCTC3110, NCTC8533, CN1793, and CN1795) supported PCR amplification of a product matching the expected size of a product from the IS 115 1 - 2 - cpb region of ATCC 3626 (data not shown).

    Article Title: Characterization of Virulence Plasmid Diversity among Clostridium perfringens Type B Isolates ▿
    Article Snippet: .. Based upon the ATCC 3626 sequence released by JCVI, a possible linkage between the cpb gene and IS 115 1 -2 insertion sequences in other C. perfringens type B isolates was investigated by PCR using primers cpb-F (5′-CTTGAAGAGTCAACAGATTGAT-3′) and IS1151-R (5′-GCTGCTAAAGTCTCTACTAG-3′). .. PCR conditions for these amplifications were as follows: 94°C for 3 min; 35 cycles of 94°C for 1 min, 55°C for 1 min, and 68°C for 4 min; and a single extension at 68°C for 10 min. PCR products were run on a 1% gel and stained with ethidium bromide for visualization.

    Article Title: Characterization of Virulence Plasmid Diversity among Clostridium perfringens Type B Isolates ▿
    Article Snippet: .. Based upon the C. perfringens type B strain ATCC 3626 sequence released by the J. Craig Venter Institute (JCVI), a series of primers were then constructed (Table ) to evaluate, by overlapping PCR, the potential linkage between tpeL and cpb in type B isolates. .. PCR conditions for these amplifications were as follows: 94°C for 3 min and 35 cycles of 94°C for 1 min, 55°C for 1 min, and 68°C for 1.5 min. PCR products were run on a 1% agarose gel and stained with ethidium bromide for visualization.

    Article Title: Characterization of Virulence Plasmid Diversity among Clostridium perfringens Type B Isolates ▿
    Article Snippet: .. Bioinformatic analysis of the ATCC 3626 sequence also indicated that the tpeL gene in this type B isolate is located ∼3 kb downstream of its cpb gene (Fig. ). .. Therefore, an overlapping PCR assay was performed, using a five-pair set of primers (encoding PCR products R1 to R5 [Table ]), to assess whether a similar genetic arrangement might exist between the cpb and tpeL genes in other type B isolates.

    other:

    Article Title: Isolation of Nitrofurantoin-Resistant Mutants of Nitroreductase-Producing Clostridium sp. Strains from the Human Intestinal Tract
    Article Snippet: The MICs of nitrofurantoin for the different Clostridium species were as follows: ≤6 μg/ml ( C. perfringens ATCC 3626), ≤8 μg/ml ( C. paraputrificum NR1), ≤5 μg/ml ( Clostridium sp. strain NR8), ≤4 μg/ml ( Clostridium sp. strain NR9), and ≤2 μg/ml ( C. leptum NP15).

    Expressing:

    Article Title: Oligomerization of Clostridium perfringens Epsilon Toxin Is Dependent upon Caveolins 1 and 2
    Article Snippet: .. ε-toxin Expression and Purification The gene encoding epsilon prototoxin, etxB , from C. perfringens type B strain ATCC 3626 was cloned into plasmid pET22b (Novagen). .. This placed the etxB gene under the regulation of the bacteriophage T7 RNA polymerase and fused the C-terminal end of the prototoxin to a His6 affinity tag (to aid in purification of the protein).

    Polymerase Chain Reaction:

    Article Title: Characterization of Virulence Plasmid Diversity among Clostridium perfringens Type B Isolates ▿
    Article Snippet: .. Therefore, a PCR analysis was performed using primers based upon the ATCC 3626 sequence to investigate whether IS 1151 -like sequences reside upstream of the cpb gene in other type B isolates. .. In this PCR, all four surveyed type B isolates (NCTC3110, NCTC8533, CN1793, and CN1795) supported PCR amplification of a product matching the expected size of a product from the IS 115 1 - 2 - cpb region of ATCC 3626 (data not shown).

    Article Title: Characterization of Virulence Plasmid Diversity among Clostridium perfringens Type B Isolates ▿
    Article Snippet: .. Based upon the ATCC 3626 sequence released by JCVI, a possible linkage between the cpb gene and IS 115 1 -2 insertion sequences in other C. perfringens type B isolates was investigated by PCR using primers cpb-F (5′-CTTGAAGAGTCAACAGATTGAT-3′) and IS1151-R (5′-GCTGCTAAAGTCTCTACTAG-3′). .. PCR conditions for these amplifications were as follows: 94°C for 3 min; 35 cycles of 94°C for 1 min, 55°C for 1 min, and 68°C for 4 min; and a single extension at 68°C for 10 min. PCR products were run on a 1% gel and stained with ethidium bromide for visualization.

    Article Title: Characterization of Virulence Plasmid Diversity among Clostridium perfringens Type B Isolates ▿
    Article Snippet: .. Based upon the C. perfringens type B strain ATCC 3626 sequence released by the J. Craig Venter Institute (JCVI), a series of primers were then constructed (Table ) to evaluate, by overlapping PCR, the potential linkage between tpeL and cpb in type B isolates. .. PCR conditions for these amplifications were as follows: 94°C for 3 min and 35 cycles of 94°C for 1 min, 55°C for 1 min, and 68°C for 1.5 min. PCR products were run on a 1% agarose gel and stained with ethidium bromide for visualization.

    Plasmid Preparation:

    Article Title: Oligomerization of Clostridium perfringens Epsilon Toxin Is Dependent upon Caveolins 1 and 2
    Article Snippet: .. ε-toxin Expression and Purification The gene encoding epsilon prototoxin, etxB , from C. perfringens type B strain ATCC 3626 was cloned into plasmid pET22b (Novagen). .. This placed the etxB gene under the regulation of the bacteriophage T7 RNA polymerase and fused the C-terminal end of the prototoxin to a His6 affinity tag (to aid in purification of the protein).

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    ATCC health bei research resources repository
    Analysis of a <t>TCRV</t> NP with GPPT residues important for anti-IFN activity. A , alignment of protein sequences near the DLQL peptide stretch of the TCRV NP reference sequence ( REF ) to the sequences derived from <t>BEI</t> resources ( BEI ) and ATCC TCRV viral stocks. B–D , measurements of IFNβ reporter activity during expression of codon-optimized (reference sequence) TCRV NP with the DLQL sequence (TCRV NP) or containing GPPT in place of the DLQL residues (TNP-GPPT). Induction of the IFNβ promoter was performed by infection with SeV ( B ), transfection with 10 μg/ml low molecular weight poly(I:C) ( C ), or with 250 ng of purified TCRV RNA ( D ) following methods described under “Experimental Procedures.” E , increasing amounts of the vTNP, TNP-GPPT, and PICV NP ranging from 0.1 and 1 μg of plasmid DNA were transfected into 293T cells and subjected to the IFN activation assay explained under “Experimental Procedures.” The positive control was set as 100% activation, and the rest of the conditions were normalized to this value. Shown are the means of three experiments performed in triplicate (**, p
    Health Bei Research Resources Repository, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/health bei research resources repository/product/ATCC
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    health bei research resources repository - by Bioz Stars, 2020-05
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    bei  (ATCC)
    86
    ATCC bei
    Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on <t>BEI</t> Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) <t>MARV</t> plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.
    Bei, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bei/product/ATCC
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bei - by Bioz Stars, 2020-05
    86/100 stars
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    85
    ATCC bei tcrv stock
    Analysis of a <t>TCRV</t> NP with GPPT residues important for anti-IFN activity. A , alignment of protein sequences near the DLQL peptide stretch of the TCRV NP reference sequence ( REF ) to the sequences derived from <t>BEI</t> resources ( BEI ) and ATCC TCRV viral stocks. B–D , measurements of IFNβ reporter activity during expression of codon-optimized (reference sequence) TCRV NP with the DLQL sequence (TCRV NP) or containing GPPT in place of the DLQL residues (TNP-GPPT). Induction of the IFNβ promoter was performed by infection with SeV ( B ), transfection with 10 μg/ml low molecular weight poly(I:C) ( C ), or with 250 ng of purified TCRV RNA ( D ) following methods described under “Experimental Procedures.” E , increasing amounts of the vTNP, TNP-GPPT, and PICV NP ranging from 0.1 and 1 μg of plasmid DNA were transfected into 293T cells and subjected to the IFN activation assay explained under “Experimental Procedures.” The positive control was set as 100% activation, and the rest of the conditions were normalized to this value. Shown are the means of three experiments performed in triplicate (**, p
    Bei Tcrv Stock, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bei tcrv stock/product/ATCC
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    Analysis of a TCRV NP with GPPT residues important for anti-IFN activity. A , alignment of protein sequences near the DLQL peptide stretch of the TCRV NP reference sequence ( REF ) to the sequences derived from BEI resources ( BEI ) and ATCC TCRV viral stocks. B–D , measurements of IFNβ reporter activity during expression of codon-optimized (reference sequence) TCRV NP with the DLQL sequence (TCRV NP) or containing GPPT in place of the DLQL residues (TNP-GPPT). Induction of the IFNβ promoter was performed by infection with SeV ( B ), transfection with 10 μg/ml low molecular weight poly(I:C) ( C ), or with 250 ng of purified TCRV RNA ( D ) following methods described under “Experimental Procedures.” E , increasing amounts of the vTNP, TNP-GPPT, and PICV NP ranging from 0.1 and 1 μg of plasmid DNA were transfected into 293T cells and subjected to the IFN activation assay explained under “Experimental Procedures.” The positive control was set as 100% activation, and the rest of the conditions were normalized to this value. Shown are the means of three experiments performed in triplicate (**, p

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of Critical Amino Acids within the Nucleoprotein of Tacaribe Virus Important for Anti-interferon Activity *

    doi: 10.1074/jbc.M112.444760

    Figure Lengend Snippet: Analysis of a TCRV NP with GPPT residues important for anti-IFN activity. A , alignment of protein sequences near the DLQL peptide stretch of the TCRV NP reference sequence ( REF ) to the sequences derived from BEI resources ( BEI ) and ATCC TCRV viral stocks. B–D , measurements of IFNβ reporter activity during expression of codon-optimized (reference sequence) TCRV NP with the DLQL sequence (TCRV NP) or containing GPPT in place of the DLQL residues (TNP-GPPT). Induction of the IFNβ promoter was performed by infection with SeV ( B ), transfection with 10 μg/ml low molecular weight poly(I:C) ( C ), or with 250 ng of purified TCRV RNA ( D ) following methods described under “Experimental Procedures.” E , increasing amounts of the vTNP, TNP-GPPT, and PICV NP ranging from 0.1 and 1 μg of plasmid DNA were transfected into 293T cells and subjected to the IFN activation assay explained under “Experimental Procedures.” The positive control was set as 100% activation, and the rest of the conditions were normalized to this value. Shown are the means of three experiments performed in triplicate (**, p

    Article Snippet: TCRV strain 11573 was obtained through the National Institutes of Health BEI Research Resources Repository, NIAID, National Institutes of Health (NR-10175) and through ATCC (VR-1272).

    Techniques: Activity Assay, Sequencing, Derivative Assay, Expressing, Infection, Transfection, Molecular Weight, Purification, Plasmid Preparation, Activation Assay, Positive Control

    Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on BEI Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) MARV plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.

    Journal: Viruses

    Article Title: Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies

    doi: 10.3390/v4123511

    Figure Lengend Snippet: Filovirus plaques produced on Vero E6 cells from two sources are similar in appearance and titer. ( A ) EBOV plaques on ATCC Vero E6 cells plated at (1) 24 hours before assay, and (2) 72 hours before assay. EBOV plaques on BEI Vero E6 cells plated at (3) 24 hours before assay, and (4) 72 hours before assay. (5) MARV plaques on Vero cells from (5) ATCC and (6) BEI plated 24 hours before assay. ( B ) EBOV titers are similar in Vero E6 cells from ATCC and BEI when measured independently by two operators. This experiment was performed twice with up to 3 operators (data not shown), and one representative graph is shown. Each bar represents an average of 7 replicates.

    Article Snippet: MARV plaque quality on BEI and ATCC Vero E6 cells appears equivalent ( A).

    Techniques: Produced

    ( A ) EBOV titers in ATCC or BEI Vero E6 cells of various passage ages. This experiment was performed by three independent investigators, four replicates per cell type. The combined data are presented, where each bar represents 12 replicates. * indicates a significant difference in value between BEI passage 40 and passage 27 ( p = 0.0003), ** a difference between ATCC passage 54 and 28 ( p = 0.007), and *** a difference between BEI passage 40 and ATCC passage 28 ( p = 0.000002). For these experiments, p value cutoff was ≤0.008). ( B ) Analysis of EBOV titer changes in cells of various passages. The arrows point out data from passages plotted in (A).

    Journal: Viruses

    Article Title: Standardization of the Filovirus Plaque Assay for Use in Preclinical Studies

    doi: 10.3390/v4123511

    Figure Lengend Snippet: ( A ) EBOV titers in ATCC or BEI Vero E6 cells of various passage ages. This experiment was performed by three independent investigators, four replicates per cell type. The combined data are presented, where each bar represents 12 replicates. * indicates a significant difference in value between BEI passage 40 and passage 27 ( p = 0.0003), ** a difference between ATCC passage 54 and 28 ( p = 0.007), and *** a difference between BEI passage 40 and ATCC passage 28 ( p = 0.000002). For these experiments, p value cutoff was ≤0.008). ( B ) Analysis of EBOV titer changes in cells of various passages. The arrows point out data from passages plotted in (A).

    Article Snippet: MARV plaque quality on BEI and ATCC Vero E6 cells appears equivalent ( A).

    Techniques:

    Analysis of a TCRV NP with GPPT residues important for anti-IFN activity. A , alignment of protein sequences near the DLQL peptide stretch of the TCRV NP reference sequence ( REF ) to the sequences derived from BEI resources ( BEI ) and ATCC TCRV viral stocks. B–D , measurements of IFNβ reporter activity during expression of codon-optimized (reference sequence) TCRV NP with the DLQL sequence (TCRV NP) or containing GPPT in place of the DLQL residues (TNP-GPPT). Induction of the IFNβ promoter was performed by infection with SeV ( B ), transfection with 10 μg/ml low molecular weight poly(I:C) ( C ), or with 250 ng of purified TCRV RNA ( D ) following methods described under “Experimental Procedures.” E , increasing amounts of the vTNP, TNP-GPPT, and PICV NP ranging from 0.1 and 1 μg of plasmid DNA were transfected into 293T cells and subjected to the IFN activation assay explained under “Experimental Procedures.” The positive control was set as 100% activation, and the rest of the conditions were normalized to this value. Shown are the means of three experiments performed in triplicate (**, p

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of Critical Amino Acids within the Nucleoprotein of Tacaribe Virus Important for Anti-interferon Activity *

    doi: 10.1074/jbc.M112.444760

    Figure Lengend Snippet: Analysis of a TCRV NP with GPPT residues important for anti-IFN activity. A , alignment of protein sequences near the DLQL peptide stretch of the TCRV NP reference sequence ( REF ) to the sequences derived from BEI resources ( BEI ) and ATCC TCRV viral stocks. B–D , measurements of IFNβ reporter activity during expression of codon-optimized (reference sequence) TCRV NP with the DLQL sequence (TCRV NP) or containing GPPT in place of the DLQL residues (TNP-GPPT). Induction of the IFNβ promoter was performed by infection with SeV ( B ), transfection with 10 μg/ml low molecular weight poly(I:C) ( C ), or with 250 ng of purified TCRV RNA ( D ) following methods described under “Experimental Procedures.” E , increasing amounts of the vTNP, TNP-GPPT, and PICV NP ranging from 0.1 and 1 μg of plasmid DNA were transfected into 293T cells and subjected to the IFN activation assay explained under “Experimental Procedures.” The positive control was set as 100% activation, and the rest of the conditions were normalized to this value. Shown are the means of three experiments performed in triplicate (**, p

    Article Snippet: The BEI TCRV stock was derived from tissue culture (Vero) infection, whereas the ATCC TCRV stock was prepared from a newborn mouse brain inoculation.

    Techniques: Activity Assay, Sequencing, Derivative Assay, Expressing, Infection, Transfection, Molecular Weight, Purification, Plasmid Preparation, Activation Assay, Positive Control