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3 Feature Barcode Capture Sequence, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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barcoded lumavidin beads  (DiaSorin Biotechnology)
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chromium single cell 3' feature barcode library & gel bead kit (v3 chemistry)  (10X Genomics)
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Chromium Single Cell 3' Feature Barcode Library & Gel Bead Kit (V3 Chemistry), supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chromium single cell 3' feature barcode library & gel bead kit (v3 chemistry) - by Bioz Stars, 2026-04
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v2 barcoding beads  (Mission Bio)
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Mission Bio v2 barcoding beads
EASi‐seq workflow: Genome purification, microfluidics, and bioinformatics. A) Microbial cells are suspended in a hydrogel precursor solution (acrylamide monomer and N,N’‐bis (acryloyl) cystamine‐BAC crosslinker) and emulsified with fluorinated oil by passing the mixture through a syringe needle. Gelation embeds individual cells within hydrogel beads. B) Hydrogel beads are size‐selected via differential centrifugation. Beads are suspended in a density‐matching buffer (40% sucrose in PBS with 0.1% Tween 20) and centrifuged at 1000 × g for 10 min to pellet oversized beads. The supernatant is centrifuged at 3000 × g for 10 min to pellet the desired bead size (5–30 µm), which is collected as size‐selected hydrogel beads. C) Cells within hydrogel beads are lysed using a two‐step enzyme digestion. A cocktail of four enzymes digests cell walls, followed by Proteinase K treatment to digest proteins. The small pore size of the hydrogel allows diffusion of proteins and smaller molecules while immobilizing long DNA molecules. Washing removes cellular debris, leaving purified genomic DNA trapped within the beads. D) Genomic DNA within hydrogel beads is tagmented in droplets during the first Tapestri microfluidic module (bottom), followed by <t>barcoding</t> PCR in the second module (top), using barcode beads paired with each droplet. E) Sequencing the barcoded amplicons produces single‐cell shotgun reads for thousands of microbial cells. F) EASi‐seq supports high‐throughput microbiome analysis, including genome atlas construction, cluster‐based genome assembly, strain identification, and pathway analysis.
V2 Barcoding Beads, supplied by Mission Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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barcoded single cell 3′ v3.1 gel beads  (10X Genomics)
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10X Genomics barcoded single cell 3′ v3.1 gel beads
EASi‐seq workflow: Genome purification, microfluidics, and bioinformatics. A) Microbial cells are suspended in a hydrogel precursor solution (acrylamide monomer and N,N’‐bis (acryloyl) cystamine‐BAC crosslinker) and emulsified with fluorinated oil by passing the mixture through a syringe needle. Gelation embeds individual cells within hydrogel beads. B) Hydrogel beads are size‐selected via differential centrifugation. Beads are suspended in a density‐matching buffer (40% sucrose in PBS with 0.1% Tween 20) and centrifuged at 1000 × g for 10 min to pellet oversized beads. The supernatant is centrifuged at 3000 × g for 10 min to pellet the desired bead size (5–30 µm), which is collected as size‐selected hydrogel beads. C) Cells within hydrogel beads are lysed using a two‐step enzyme digestion. A cocktail of four enzymes digests cell walls, followed by Proteinase K treatment to digest proteins. The small pore size of the hydrogel allows diffusion of proteins and smaller molecules while immobilizing long DNA molecules. Washing removes cellular debris, leaving purified genomic DNA trapped within the beads. D) Genomic DNA within hydrogel beads is tagmented in droplets during the first Tapestri microfluidic module (bottom), followed by <t>barcoding</t> PCR in the second module (top), using barcode beads paired with each droplet. E) Sequencing the barcoded amplicons produces single‐cell shotgun reads for thousands of microbial cells. F) EASi‐seq supports high‐throughput microbiome analysis, including genome atlas construction, cluster‐based genome assembly, strain identification, and pathway analysis.
Barcoded Single Cell 3′ V3.1 Gel Beads, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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barcoded beads nc0927472  (Fisher Scientific)
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Fisher Scientific barcoded beads nc0927472
EASi‐seq workflow: Genome purification, microfluidics, and bioinformatics. A) Microbial cells are suspended in a hydrogel precursor solution (acrylamide monomer and N,N’‐bis (acryloyl) cystamine‐BAC crosslinker) and emulsified with fluorinated oil by passing the mixture through a syringe needle. Gelation embeds individual cells within hydrogel beads. B) Hydrogel beads are size‐selected via differential centrifugation. Beads are suspended in a density‐matching buffer (40% sucrose in PBS with 0.1% Tween 20) and centrifuged at 1000 × g for 10 min to pellet oversized beads. The supernatant is centrifuged at 3000 × g for 10 min to pellet the desired bead size (5–30 µm), which is collected as size‐selected hydrogel beads. C) Cells within hydrogel beads are lysed using a two‐step enzyme digestion. A cocktail of four enzymes digests cell walls, followed by Proteinase K treatment to digest proteins. The small pore size of the hydrogel allows diffusion of proteins and smaller molecules while immobilizing long DNA molecules. Washing removes cellular debris, leaving purified genomic DNA trapped within the beads. D) Genomic DNA within hydrogel beads is tagmented in droplets during the first Tapestri microfluidic module (bottom), followed by <t>barcoding</t> PCR in the second module (top), using barcode beads paired with each droplet. E) Sequencing the barcoded amplicons produces single‐cell shotgun reads for thousands of microbial cells. F) EASi‐seq supports high‐throughput microbiome analysis, including genome atlas construction, cluster‐based genome assembly, strain identification, and pathway analysis.
Barcoded Beads Nc0927472, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gel beads with 10x barcodes  (10X Genomics)
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10X Genomics gel beads with 10x barcodes
EASi‐seq workflow: Genome purification, microfluidics, and bioinformatics. A) Microbial cells are suspended in a hydrogel precursor solution (acrylamide monomer and N,N’‐bis (acryloyl) cystamine‐BAC crosslinker) and emulsified with fluorinated oil by passing the mixture through a syringe needle. Gelation embeds individual cells within hydrogel beads. B) Hydrogel beads are size‐selected via differential centrifugation. Beads are suspended in a density‐matching buffer (40% sucrose in PBS with 0.1% Tween 20) and centrifuged at 1000 × g for 10 min to pellet oversized beads. The supernatant is centrifuged at 3000 × g for 10 min to pellet the desired bead size (5–30 µm), which is collected as size‐selected hydrogel beads. C) Cells within hydrogel beads are lysed using a two‐step enzyme digestion. A cocktail of four enzymes digests cell walls, followed by Proteinase K treatment to digest proteins. The small pore size of the hydrogel allows diffusion of proteins and smaller molecules while immobilizing long DNA molecules. Washing removes cellular debris, leaving purified genomic DNA trapped within the beads. D) Genomic DNA within hydrogel beads is tagmented in droplets during the first Tapestri microfluidic module (bottom), followed by <t>barcoding</t> PCR in the second module (top), using barcode beads paired with each droplet. E) Sequencing the barcoded amplicons produces single‐cell shotgun reads for thousands of microbial cells. F) EASi‐seq supports high‐throughput microbiome analysis, including genome atlas construction, cluster‐based genome assembly, strain identification, and pathway analysis.
Gel Beads With 10x Barcodes, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gel bead-in-emulsion (gem) generation, barcoding and cdna preparation  (10X Genomics)
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10X Genomics gel bead-in-emulsion (gem) generation, barcoding and cdna preparation
EASi‐seq workflow: Genome purification, microfluidics, and bioinformatics. A) Microbial cells are suspended in a hydrogel precursor solution (acrylamide monomer and N,N’‐bis (acryloyl) cystamine‐BAC crosslinker) and emulsified with fluorinated oil by passing the mixture through a syringe needle. Gelation embeds individual cells within hydrogel beads. B) Hydrogel beads are size‐selected via differential centrifugation. Beads are suspended in a density‐matching buffer (40% sucrose in PBS with 0.1% Tween 20) and centrifuged at 1000 × g for 10 min to pellet oversized beads. The supernatant is centrifuged at 3000 × g for 10 min to pellet the desired bead size (5–30 µm), which is collected as size‐selected hydrogel beads. C) Cells within hydrogel beads are lysed using a two‐step enzyme digestion. A cocktail of four enzymes digests cell walls, followed by Proteinase K treatment to digest proteins. The small pore size of the hydrogel allows diffusion of proteins and smaller molecules while immobilizing long DNA molecules. Washing removes cellular debris, leaving purified genomic DNA trapped within the beads. D) Genomic DNA within hydrogel beads is tagmented in droplets during the first Tapestri microfluidic module (bottom), followed by <t>barcoding</t> PCR in the second module (top), using barcode beads paired with each droplet. E) Sequencing the barcoded amplicons produces single‐cell shotgun reads for thousands of microbial cells. F) EASi‐seq supports high‐throughput microbiome analysis, including genome atlas construction, cluster‐based genome assembly, strain identification, and pathway analysis.
Gel Bead In Emulsion (Gem) Generation, Barcoding And Cdna Preparation, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gel bead-in-emulsion (gem) generation, barcoding and cdna preparation - by Bioz Stars, 2026-04
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lyophilized barcoded magnetic beads conjugated with dsdna antigens  (Applied BioCode)
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Applied BioCode lyophilized barcoded magnetic beads conjugated with dsdna antigens
EASi‐seq workflow: Genome purification, microfluidics, and bioinformatics. A) Microbial cells are suspended in a hydrogel precursor solution (acrylamide monomer and N,N’‐bis (acryloyl) cystamine‐BAC crosslinker) and emulsified with fluorinated oil by passing the mixture through a syringe needle. Gelation embeds individual cells within hydrogel beads. B) Hydrogel beads are size‐selected via differential centrifugation. Beads are suspended in a density‐matching buffer (40% sucrose in PBS with 0.1% Tween 20) and centrifuged at 1000 × g for 10 min to pellet oversized beads. The supernatant is centrifuged at 3000 × g for 10 min to pellet the desired bead size (5–30 µm), which is collected as size‐selected hydrogel beads. C) Cells within hydrogel beads are lysed using a two‐step enzyme digestion. A cocktail of four enzymes digests cell walls, followed by Proteinase K treatment to digest proteins. The small pore size of the hydrogel allows diffusion of proteins and smaller molecules while immobilizing long DNA molecules. Washing removes cellular debris, leaving purified genomic DNA trapped within the beads. D) Genomic DNA within hydrogel beads is tagmented in droplets during the first Tapestri microfluidic module (bottom), followed by <t>barcoding</t> PCR in the second module (top), using barcode beads paired with each droplet. E) Sequencing the barcoded amplicons produces single‐cell shotgun reads for thousands of microbial cells. F) EASi‐seq supports high‐throughput microbiome analysis, including genome atlas construction, cluster‐based genome assembly, strain identification, and pathway analysis.
Lyophilized Barcoded Magnetic Beads Conjugated With Dsdna Antigens, supplied by Applied BioCode, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lyophilized barcoded magnetic beads conjugated with dsdna antigens - by Bioz Stars, 2026-04
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EASi‐seq workflow: Genome purification, microfluidics, and bioinformatics. A) Microbial cells are suspended in a hydrogel precursor solution (acrylamide monomer and N,N’‐bis (acryloyl) cystamine‐BAC crosslinker) and emulsified with fluorinated oil by passing the mixture through a syringe needle. Gelation embeds individual cells within hydrogel beads. B) Hydrogel beads are size‐selected via differential centrifugation. Beads are suspended in a density‐matching buffer (40% sucrose in PBS with 0.1% Tween 20) and centrifuged at 1000 × g for 10 min to pellet oversized beads. The supernatant is centrifuged at 3000 × g for 10 min to pellet the desired bead size (5–30 µm), which is collected as size‐selected hydrogel beads. C) Cells within hydrogel beads are lysed using a two‐step enzyme digestion. A cocktail of four enzymes digests cell walls, followed by Proteinase K treatment to digest proteins. The small pore size of the hydrogel allows diffusion of proteins and smaller molecules while immobilizing long DNA molecules. Washing removes cellular debris, leaving purified genomic DNA trapped within the beads. D) Genomic DNA within hydrogel beads is tagmented in droplets during the first Tapestri microfluidic module (bottom), followed by barcoding PCR in the second module (top), using barcode beads paired with each droplet. E) Sequencing the barcoded amplicons produces single‐cell shotgun reads for thousands of microbial cells. F) EASi‐seq supports high‐throughput microbiome analysis, including genome atlas construction, cluster‐based genome assembly, strain identification, and pathway analysis.

Journal: Advanced Science

Article Title: Microbiome Single Cell Atlases Generated with a Commercial Instrument

doi: 10.1002/advs.202409338

Figure Lengend Snippet: EASi‐seq workflow: Genome purification, microfluidics, and bioinformatics. A) Microbial cells are suspended in a hydrogel precursor solution (acrylamide monomer and N,N’‐bis (acryloyl) cystamine‐BAC crosslinker) and emulsified with fluorinated oil by passing the mixture through a syringe needle. Gelation embeds individual cells within hydrogel beads. B) Hydrogel beads are size‐selected via differential centrifugation. Beads are suspended in a density‐matching buffer (40% sucrose in PBS with 0.1% Tween 20) and centrifuged at 1000 × g for 10 min to pellet oversized beads. The supernatant is centrifuged at 3000 × g for 10 min to pellet the desired bead size (5–30 µm), which is collected as size‐selected hydrogel beads. C) Cells within hydrogel beads are lysed using a two‐step enzyme digestion. A cocktail of four enzymes digests cell walls, followed by Proteinase K treatment to digest proteins. The small pore size of the hydrogel allows diffusion of proteins and smaller molecules while immobilizing long DNA molecules. Washing removes cellular debris, leaving purified genomic DNA trapped within the beads. D) Genomic DNA within hydrogel beads is tagmented in droplets during the first Tapestri microfluidic module (bottom), followed by barcoding PCR in the second module (top), using barcode beads paired with each droplet. E) Sequencing the barcoded amplicons produces single‐cell shotgun reads for thousands of microbial cells. F) EASi‐seq supports high‐throughput microbiome analysis, including genome atlas construction, cluster‐based genome assembly, strain identification, and pathway analysis.

Article Snippet: 200 μL Mission Bio V2 barcoding beads washed with Tris buffer (pH 8.0) and resuspended in 10 m m Tris buffer containing 3.75% tween 20, 2.5 m m MgCl 2 , 0.625 mg mL −1 BSA.

Techniques: Purification, Centrifugation, Pore Size, Diffusion-based Assay, Sequencing, High Throughput Screening Assay