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1) Product Images from "Neurotensin receptor type 2 protects B-cell chronic lymphocytic leukemia cells from apoptosis"
Article Title: Neurotensin receptor type 2 protects B-cell chronic lymphocytic leukemia cells from apoptosis
Figure Legend Snippet: NTSR2 phosphorylation in B-CLL and recruitment of G i α proteins. ( a ) Representative western blot analysis of Src phosphorylation in MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) in the presence or absence of BDNF (100 ng/ml), pertussis toxin (PTX, 200 ng/ml) or K252a (100 nM) for 24 h. ( b ) Ratio of phosphorylated Src vs pan-Src protein, normalized against actin. Values are means±s.e.m. of three independent experiments in a.u. ( c , d ). After immunoprecipitation (IP) of NTSR2 from MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) or not (EV), or from B-CLL cells in the presence or absence of BDNF (100 ng/ml) or NTS (40 μ M /ml), the immunocomplexes were immunoblotted (IB) with anti-G i α1/2 antibodies. ( e , f ) After immunoprecipitation (IP) of anti-pan-phosphoprotein was performed on MEC-1 cells overexpressing NTSR2 and B-CLL cells in the presence or absence of BDNF (100 ng/ml) or SR142948A (67 μ M ), the phosphorylation of NTSR2 was detected by immunoblotting (IB) with anti-NTSR2 antibodies. ( g ) Apoptotic ratio, assessed by cell death ELISA, in B-CLL in the presence or absence of SR142948A (67 μ M ) for 24 h. Values are mean ratios of apoptotic cells (±s.e.m.) of three independent experiments from different patients ( n =3). Significant P -values are indicated in the graphs * P
Techniques Used: Western Blot, Immunoprecipitation, Enzyme-linked Immunosorbent Assay