bdnf quantification plasma bdnf  (alomone labs)


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    alomone labs bdnf quantification plasma bdnf
    NTSR2 phosphorylation in B-CLL and recruitment of G i α proteins. ( a ) Representative western blot analysis of Src phosphorylation in MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) in the presence or absence of <t>BDNF</t> (100 ng/ml), pertussis toxin (PTX, 200 ng/ml) or K252a (100 nM) for 24 h. ( b ) Ratio of phosphorylated Src vs pan-Src protein, normalized against actin. Values are means±s.e.m. of three independent experiments in a.u. ( c , d ). After immunoprecipitation (IP) of NTSR2 from MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) or not (EV), or from B-CLL cells in the presence or absence of BDNF (100 ng/ml) or <t>NTS</t> (40 μ M /ml), the immunocomplexes were immunoblotted (IB) with anti-G i α1/2 antibodies. ( e , f ) After immunoprecipitation (IP) of anti-pan-phosphoprotein was performed on MEC-1 cells overexpressing NTSR2 and B-CLL cells in the presence or absence of BDNF (100 ng/ml) or SR142948A (67 μ M ), the phosphorylation of NTSR2 was detected by immunoblotting (IB) with anti-NTSR2 antibodies. ( g ) Apoptotic ratio, assessed by cell death ELISA, in B-CLL in the presence or absence of SR142948A (67 μ M ) for 24 h. Values are mean ratios of apoptotic cells (±s.e.m.) of three independent experiments from different patients ( n =3). Significant P -values are indicated in the graphs * P
    Bdnf Quantification Plasma Bdnf, supplied by alomone labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bdnf quantification plasma bdnf/product/alomone labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bdnf quantification plasma bdnf - by Bioz Stars, 2022-09
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    1) Product Images from "Neurotensin receptor type 2 protects B-cell chronic lymphocytic leukemia cells from apoptosis"

    Article Title: Neurotensin receptor type 2 protects B-cell chronic lymphocytic leukemia cells from apoptosis

    Journal: Oncogene

    doi: 10.1038/onc.2017.365

    NTSR2 phosphorylation in B-CLL and recruitment of G i α proteins. ( a ) Representative western blot analysis of Src phosphorylation in MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) in the presence or absence of BDNF (100 ng/ml), pertussis toxin (PTX, 200 ng/ml) or K252a (100 nM) for 24 h. ( b ) Ratio of phosphorylated Src vs pan-Src protein, normalized against actin. Values are means±s.e.m. of three independent experiments in a.u. ( c , d ). After immunoprecipitation (IP) of NTSR2 from MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) or not (EV), or from B-CLL cells in the presence or absence of BDNF (100 ng/ml) or NTS (40 μ M /ml), the immunocomplexes were immunoblotted (IB) with anti-G i α1/2 antibodies. ( e , f ) After immunoprecipitation (IP) of anti-pan-phosphoprotein was performed on MEC-1 cells overexpressing NTSR2 and B-CLL cells in the presence or absence of BDNF (100 ng/ml) or SR142948A (67 μ M ), the phosphorylation of NTSR2 was detected by immunoblotting (IB) with anti-NTSR2 antibodies. ( g ) Apoptotic ratio, assessed by cell death ELISA, in B-CLL in the presence or absence of SR142948A (67 μ M ) for 24 h. Values are mean ratios of apoptotic cells (±s.e.m.) of three independent experiments from different patients ( n =3). Significant P -values are indicated in the graphs * P
    Figure Legend Snippet: NTSR2 phosphorylation in B-CLL and recruitment of G i α proteins. ( a ) Representative western blot analysis of Src phosphorylation in MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) in the presence or absence of BDNF (100 ng/ml), pertussis toxin (PTX, 200 ng/ml) or K252a (100 nM) for 24 h. ( b ) Ratio of phosphorylated Src vs pan-Src protein, normalized against actin. Values are means±s.e.m. of three independent experiments in a.u. ( c , d ). After immunoprecipitation (IP) of NTSR2 from MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) or not (EV), or from B-CLL cells in the presence or absence of BDNF (100 ng/ml) or NTS (40 μ M /ml), the immunocomplexes were immunoblotted (IB) with anti-G i α1/2 antibodies. ( e , f ) After immunoprecipitation (IP) of anti-pan-phosphoprotein was performed on MEC-1 cells overexpressing NTSR2 and B-CLL cells in the presence or absence of BDNF (100 ng/ml) or SR142948A (67 μ M ), the phosphorylation of NTSR2 was detected by immunoblotting (IB) with anti-NTSR2 antibodies. ( g ) Apoptotic ratio, assessed by cell death ELISA, in B-CLL in the presence or absence of SR142948A (67 μ M ) for 24 h. Values are mean ratios of apoptotic cells (±s.e.m.) of three independent experiments from different patients ( n =3). Significant P -values are indicated in the graphs * P

    Techniques Used: Western Blot, Immunoprecipitation, Enzyme-linked Immunosorbent Assay

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    alomone labs bdnf quantification plasma bdnf
    NTSR2 phosphorylation in B-CLL and recruitment of G i α proteins. ( a ) Representative western blot analysis of Src phosphorylation in MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) in the presence or absence of <t>BDNF</t> (100 ng/ml), pertussis toxin (PTX, 200 ng/ml) or K252a (100 nM) for 24 h. ( b ) Ratio of phosphorylated Src vs pan-Src protein, normalized against actin. Values are means±s.e.m. of three independent experiments in a.u. ( c , d ). After immunoprecipitation (IP) of NTSR2 from MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) or not (EV), or from B-CLL cells in the presence or absence of BDNF (100 ng/ml) or <t>NTS</t> (40 μ M /ml), the immunocomplexes were immunoblotted (IB) with anti-G i α1/2 antibodies. ( e , f ) After immunoprecipitation (IP) of anti-pan-phosphoprotein was performed on MEC-1 cells overexpressing NTSR2 and B-CLL cells in the presence or absence of BDNF (100 ng/ml) or SR142948A (67 μ M ), the phosphorylation of NTSR2 was detected by immunoblotting (IB) with anti-NTSR2 antibodies. ( g ) Apoptotic ratio, assessed by cell death ELISA, in B-CLL in the presence or absence of SR142948A (67 μ M ) for 24 h. Values are mean ratios of apoptotic cells (±s.e.m.) of three independent experiments from different patients ( n =3). Significant P -values are indicated in the graphs * P
    Bdnf Quantification Plasma Bdnf, supplied by alomone labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bdnf quantification plasma bdnf/product/alomone labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bdnf quantification plasma bdnf - by Bioz Stars, 2022-09
    86/100 stars
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    NTSR2 phosphorylation in B-CLL and recruitment of G i α proteins. ( a ) Representative western blot analysis of Src phosphorylation in MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) in the presence or absence of BDNF (100 ng/ml), pertussis toxin (PTX, 200 ng/ml) or K252a (100 nM) for 24 h. ( b ) Ratio of phosphorylated Src vs pan-Src protein, normalized against actin. Values are means±s.e.m. of three independent experiments in a.u. ( c , d ). After immunoprecipitation (IP) of NTSR2 from MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) or not (EV), or from B-CLL cells in the presence or absence of BDNF (100 ng/ml) or NTS (40 μ M /ml), the immunocomplexes were immunoblotted (IB) with anti-G i α1/2 antibodies. ( e , f ) After immunoprecipitation (IP) of anti-pan-phosphoprotein was performed on MEC-1 cells overexpressing NTSR2 and B-CLL cells in the presence or absence of BDNF (100 ng/ml) or SR142948A (67 μ M ), the phosphorylation of NTSR2 was detected by immunoblotting (IB) with anti-NTSR2 antibodies. ( g ) Apoptotic ratio, assessed by cell death ELISA, in B-CLL in the presence or absence of SR142948A (67 μ M ) for 24 h. Values are mean ratios of apoptotic cells (±s.e.m.) of three independent experiments from different patients ( n =3). Significant P -values are indicated in the graphs * P

    Journal: Oncogene

    Article Title: Neurotensin receptor type 2 protects B-cell chronic lymphocytic leukemia cells from apoptosis

    doi: 10.1038/onc.2017.365

    Figure Lengend Snippet: NTSR2 phosphorylation in B-CLL and recruitment of G i α proteins. ( a ) Representative western blot analysis of Src phosphorylation in MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) in the presence or absence of BDNF (100 ng/ml), pertussis toxin (PTX, 200 ng/ml) or K252a (100 nM) for 24 h. ( b ) Ratio of phosphorylated Src vs pan-Src protein, normalized against actin. Values are means±s.e.m. of three independent experiments in a.u. ( c , d ). After immunoprecipitation (IP) of NTSR2 from MEC-1 cells overexpressing NTSR2 (pCMV6-NTSR2) or not (EV), or from B-CLL cells in the presence or absence of BDNF (100 ng/ml) or NTS (40 μ M /ml), the immunocomplexes were immunoblotted (IB) with anti-G i α1/2 antibodies. ( e , f ) After immunoprecipitation (IP) of anti-pan-phosphoprotein was performed on MEC-1 cells overexpressing NTSR2 and B-CLL cells in the presence or absence of BDNF (100 ng/ml) or SR142948A (67 μ M ), the phosphorylation of NTSR2 was detected by immunoblotting (IB) with anti-NTSR2 antibodies. ( g ) Apoptotic ratio, assessed by cell death ELISA, in B-CLL in the presence or absence of SR142948A (67 μ M ) for 24 h. Values are mean ratios of apoptotic cells (±s.e.m.) of three independent experiments from different patients ( n =3). Significant P -values are indicated in the graphs * P

    Article Snippet: Plasma NTS and BDNF quantification Plasma BDNF and NTS levels were measured using commercial ELISA kits: BDNF Emax ELISA ImmunoAssay System (Promega, WI, USA) and Human Neurotensin, NT ELISA Kit (CUSABIO Life Science, College Park, MD, USA).

    Techniques: Western Blot, Immunoprecipitation, Enzyme-linked Immunosorbent Assay