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Cell Signaling Technology Inc bcr abl expression
Bcr Abl Expression, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bcr abl expression/product/Cell Signaling Technology Inc
Average 95 stars, based on 2 article reviews
Price from $9.99 to $1999.99
bcr abl expression - by Bioz Stars, 2020-09
95/100 stars

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Western Blot:

Article Title: The Rho-specific Guanine Nucleotide Exchange Factor Dbs Regulates Breast Cancer Cell Migration *
Article Snippet: .. Antibodies used for Western blots include: anti-HA (Berkeley Antibody Co., Inc.), anti-Crk (BD Transduction Laboratories), anti-p-Crk (pY207, Cell Signaling), anti-p130Cas (BD Transduction), anti-phospho-p130Cas (pY410, Cell Signaling), anti-FAK (Santa Cruz, sc-557), anti-phospho-FAK (pY397, BIOSOURCE), anti-Ack1 (Santa Cruz, sc-323), anti-Cdc42 (Santa Cruz, sc-8401), anti-Rac1 (BD Transduction Laboratories), anti-RhoA (Santa Cruz, sc-418), anti-Src (Santa Cruz, sc-18), anti-phospho-Src (pY418, Calbiochem), anti-Bcr (Cell Signaling), anti-Tiam1 (Santa Cruz, sc-872), anti-Ost (Santa Cruz, sc-901), and anti-actin (Santa Cruz, sc-8432). .. Statistical analyses were performed by one-way analysis of variance followed by the Tukey HSD (honestly significant difference) test for multiple comparisons using JMP statistical software. p values of < 0.05 were considered significant.

Incubation:

Article Title: Ovatodiolide targets chronic myeloid leukemia stem cells by epigenetically upregulating hsa-miR-155, suppressing the BCR-ABL fusion gene and dysregulating the PI3K/AKT/mTOR pathway
Article Snippet: .. Incubation of the membranes in 5% skimmed milk - Tris-buffered saline with Tween 20 (TBST) for 1 h to block non-specific binding, was followed by incubation overnight at 4°C with the antibodies against Bcr-Abl (1 : 1000, Cell Signaling Technology), Oct4 (1 : 1000, Santa Cruz), Sox2 (1 : 1000, Cell Signaling Technology), p-CrkL (1 : 1000, Cell Signaling Technology), Stat5 (1 : 1000, Santa Cruz), p-Stat5 (1 : 1000, Santa Cruz), MDR (1 : 1000, Cell Signaling Technology), CD133 (1 : 1000, Cell Signaling Technology), pAkt (1 : 1000, Santa Cruz), Akt (1 : 1000, Santa Cruz), p-PI3K (1 : 1000, Cell Signaling Technology), PI3K (1 : 1000, Cell Signaling Technology), p-mTOR (1 : 1000, Cell Signaling Technology), CD44 (1 : 1000, Santa Cruz), and β-actin (1 : 500, Santa Cruz). .. The membranes were then washed and incubated in appropriate horseradish peroxidise (HRP)-conjugated secondary antibodies for 1 h at room temperature, then washed with PBS 3 times.

Binding Assay:

Article Title: Ovatodiolide targets chronic myeloid leukemia stem cells by epigenetically upregulating hsa-miR-155, suppressing the BCR-ABL fusion gene and dysregulating the PI3K/AKT/mTOR pathway
Article Snippet: .. Incubation of the membranes in 5% skimmed milk - Tris-buffered saline with Tween 20 (TBST) for 1 h to block non-specific binding, was followed by incubation overnight at 4°C with the antibodies against Bcr-Abl (1 : 1000, Cell Signaling Technology), Oct4 (1 : 1000, Santa Cruz), Sox2 (1 : 1000, Cell Signaling Technology), p-CrkL (1 : 1000, Cell Signaling Technology), Stat5 (1 : 1000, Santa Cruz), p-Stat5 (1 : 1000, Santa Cruz), MDR (1 : 1000, Cell Signaling Technology), CD133 (1 : 1000, Cell Signaling Technology), pAkt (1 : 1000, Santa Cruz), Akt (1 : 1000, Santa Cruz), p-PI3K (1 : 1000, Cell Signaling Technology), PI3K (1 : 1000, Cell Signaling Technology), p-mTOR (1 : 1000, Cell Signaling Technology), CD44 (1 : 1000, Santa Cruz), and β-actin (1 : 500, Santa Cruz). .. The membranes were then washed and incubated in appropriate horseradish peroxidise (HRP)-conjugated secondary antibodies for 1 h at room temperature, then washed with PBS 3 times.

Blocking Assay:

Article Title: Ovatodiolide targets chronic myeloid leukemia stem cells by epigenetically upregulating hsa-miR-155, suppressing the BCR-ABL fusion gene and dysregulating the PI3K/AKT/mTOR pathway
Article Snippet: .. Incubation of the membranes in 5% skimmed milk - Tris-buffered saline with Tween 20 (TBST) for 1 h to block non-specific binding, was followed by incubation overnight at 4°C with the antibodies against Bcr-Abl (1 : 1000, Cell Signaling Technology), Oct4 (1 : 1000, Santa Cruz), Sox2 (1 : 1000, Cell Signaling Technology), p-CrkL (1 : 1000, Cell Signaling Technology), Stat5 (1 : 1000, Santa Cruz), p-Stat5 (1 : 1000, Santa Cruz), MDR (1 : 1000, Cell Signaling Technology), CD133 (1 : 1000, Cell Signaling Technology), pAkt (1 : 1000, Santa Cruz), Akt (1 : 1000, Santa Cruz), p-PI3K (1 : 1000, Cell Signaling Technology), PI3K (1 : 1000, Cell Signaling Technology), p-mTOR (1 : 1000, Cell Signaling Technology), CD44 (1 : 1000, Santa Cruz), and β-actin (1 : 500, Santa Cruz). .. The membranes were then washed and incubated in appropriate horseradish peroxidise (HRP)-conjugated secondary antibodies for 1 h at room temperature, then washed with PBS 3 times.

Nucleic Acid Electrophoresis:

Article Title: A dynamic N6-methyladenosine methylome regulates intrinsic and acquired resistance to tyrosine kinase inhibitors
Article Snippet: .. The proteins were resolved by sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis, transferred onto PVDF membranes (GE Healthcare #10600023), blocked by 5% non-fat milk followed by probing with anti-STAT5 (1:1000, Cell Signaling #9363), anti-p-STAT5 (1:1000, Cell Signaling #9351), anti-KIT (1:500, Cell Signaling #3074), anti-p-KIT (1:500, Cell Signaling #3391), anti-FLT3 (1:500, Cell Signaling #3462), anti-p-FLT3 (1:500, Cell Signaling #3464), anti-BCR (1:500, Cell Signaling #3902), anti-p-ABL (1:500, Cell Signaling #2865), anti-β-Actin (1:1000, Santa Cruz #sc-47778), anti-MERTK (1:1000, Santa Cruz #sc-365499), anti-FTO (1:2000, AdipoGen #AG-20A-0064), anti-METTL3 (1:2000, Proteintech #15073-1-AP), anti-ALKBH5 (1:500, Abcam #ab69325), anti-METTL14 (1:1000, Abcam #ab98166), anti-WTAP (1:2000, Abcam #ab195380), and anti-BCL-2 (1:500, EMD Millipore #05-729) antibodies. .. The secondary antibodies are: horse anti-mouse IgG, HRP-linked antibody (Cell Signaling #7076), goat anti-rabbit IgG, HRP-linked antibody (Cell Signaling #7074), rabbit anti-goat IgG HRP-linked antibody (Invitrogen #31402).

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    Cell Signaling Technology Inc bcr abl expression
    Bcr Abl Expression, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bcr abl expression/product/Cell Signaling Technology Inc
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bcr abl expression - by Bioz Stars, 2020-09
    95/100 stars
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