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nsc base medium  (STEMCELL Technologies Inc)
86
STEMCELL Technologies Inc nsc base medium
Characterization <t>of</t> <t>hNSCs</t> and hNSC-EVs. (A) Morphology of first- and third-passage hNSCs. Scale bar: 100 μm. (B) The hNSCs were positive for neural stem cell markers. Representative images of hNSCs showing PAX6 in purple, Nestin in purple, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (C) Analysis of <t>NSC</t> multipotentiality via a standard differentiation assay showing upregulation of neuronal and neuroglial markers. Representative images of hNSCs showing βIII-tubulin (neuron) in white, GFAP (neuroglia) in green, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (D) Nanoparticle tracking analysis showing the diameters of the hNSC-EVs. (E) TEM showing hNSC-EV morphology. Scale bar: 100 nm. (F) Immunoblot for CD9, CD63, TSG101, and calnexin expression by hNSC-EVs. (G) hNSC-EV internalization by HUVECs, mouse hippocampal neuronal cells (HT22), and mouse microglial cells (BV2). Representative confocal microscope images of cells with green-labeled cytoplasmic and DAPI-stained nuclei (blue) 6 hours after exposure to PKH26-labeled hNSC-EVs (red). Scale bar: 5 μm. All experiments were performed at least three times. DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; hNSC: human neural stem cell; hNSC-EVs: hNSC-derived extracellular vesicles; HUVEC: human umbilical vein endothelial cells; NTA: nanoparticle tracking analysis.
Nsc Base Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nsc base medium/product/STEMCELL Technologies Inc
Average 86 stars, based on 1 article reviews
nsc base medium - by Bioz Stars, 2025-07
86/100 stars
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macrophage medium based osteoclast differentiation medium  (MedChemExpress)
86
MedChemExpress macrophage medium based osteoclast differentiation medium
Characterization <t>of</t> <t>hNSCs</t> and hNSC-EVs. (A) Morphology of first- and third-passage hNSCs. Scale bar: 100 μm. (B) The hNSCs were positive for neural stem cell markers. Representative images of hNSCs showing PAX6 in purple, Nestin in purple, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (C) Analysis of <t>NSC</t> multipotentiality via a standard differentiation assay showing upregulation of neuronal and neuroglial markers. Representative images of hNSCs showing βIII-tubulin (neuron) in white, GFAP (neuroglia) in green, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (D) Nanoparticle tracking analysis showing the diameters of the hNSC-EVs. (E) TEM showing hNSC-EV morphology. Scale bar: 100 nm. (F) Immunoblot for CD9, CD63, TSG101, and calnexin expression by hNSC-EVs. (G) hNSC-EV internalization by HUVECs, mouse hippocampal neuronal cells (HT22), and mouse microglial cells (BV2). Representative confocal microscope images of cells with green-labeled cytoplasmic and DAPI-stained nuclei (blue) 6 hours after exposure to PKH26-labeled hNSC-EVs (red). Scale bar: 5 μm. All experiments were performed at least three times. DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; hNSC: human neural stem cell; hNSC-EVs: hNSC-derived extracellular vesicles; HUVEC: human umbilical vein endothelial cells; NTA: nanoparticle tracking analysis.
Macrophage Medium Based Osteoclast Differentiation Medium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/macrophage medium based osteoclast differentiation medium/product/MedChemExpress
Average 86 stars, based on 1 article reviews
macrophage medium based osteoclast differentiation medium - by Bioz Stars, 2025-07
86/100 stars
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base medium  (Corning Life Sciences)
86
Corning Life Sciences base medium
Characterization <t>of</t> <t>hNSCs</t> and hNSC-EVs. (A) Morphology of first- and third-passage hNSCs. Scale bar: 100 μm. (B) The hNSCs were positive for neural stem cell markers. Representative images of hNSCs showing PAX6 in purple, Nestin in purple, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (C) Analysis of <t>NSC</t> multipotentiality via a standard differentiation assay showing upregulation of neuronal and neuroglial markers. Representative images of hNSCs showing βIII-tubulin (neuron) in white, GFAP (neuroglia) in green, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (D) Nanoparticle tracking analysis showing the diameters of the hNSC-EVs. (E) TEM showing hNSC-EV morphology. Scale bar: 100 nm. (F) Immunoblot for CD9, CD63, TSG101, and calnexin expression by hNSC-EVs. (G) hNSC-EV internalization by HUVECs, mouse hippocampal neuronal cells (HT22), and mouse microglial cells (BV2). Representative confocal microscope images of cells with green-labeled cytoplasmic and DAPI-stained nuclei (blue) 6 hours after exposure to PKH26-labeled hNSC-EVs (red). Scale bar: 5 μm. All experiments were performed at least three times. DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; hNSC: human neural stem cell; hNSC-EVs: hNSC-derived extracellular vesicles; HUVEC: human umbilical vein endothelial cells; NTA: nanoparticle tracking analysis.
Base Medium, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/base medium/product/Corning Life Sciences
Average 86 stars, based on 1 article reviews
base medium - by Bioz Stars, 2025-07
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methylcellulose based medium  (STEMCELL Technologies Inc)
86
STEMCELL Technologies Inc methylcellulose based medium
Characterization <t>of</t> <t>hNSCs</t> and hNSC-EVs. (A) Morphology of first- and third-passage hNSCs. Scale bar: 100 μm. (B) The hNSCs were positive for neural stem cell markers. Representative images of hNSCs showing PAX6 in purple, Nestin in purple, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (C) Analysis of <t>NSC</t> multipotentiality via a standard differentiation assay showing upregulation of neuronal and neuroglial markers. Representative images of hNSCs showing βIII-tubulin (neuron) in white, GFAP (neuroglia) in green, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (D) Nanoparticle tracking analysis showing the diameters of the hNSC-EVs. (E) TEM showing hNSC-EV morphology. Scale bar: 100 nm. (F) Immunoblot for CD9, CD63, TSG101, and calnexin expression by hNSC-EVs. (G) hNSC-EV internalization by HUVECs, mouse hippocampal neuronal cells (HT22), and mouse microglial cells (BV2). Representative confocal microscope images of cells with green-labeled cytoplasmic and DAPI-stained nuclei (blue) 6 hours after exposure to PKH26-labeled hNSC-EVs (red). Scale bar: 5 μm. All experiments were performed at least three times. DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; hNSC: human neural stem cell; hNSC-EVs: hNSC-derived extracellular vesicles; HUVEC: human umbilical vein endothelial cells; NTA: nanoparticle tracking analysis.
Methylcellulose Based Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/methylcellulose based medium/product/STEMCELL Technologies Inc
Average 86 stars, based on 1 article reviews
methylcellulose based medium - by Bioz Stars, 2025-07
86/100 stars
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xf dmem base medium  (Agilent technologies)
86
Agilent technologies xf dmem base medium
Characterization <t>of</t> <t>hNSCs</t> and hNSC-EVs. (A) Morphology of first- and third-passage hNSCs. Scale bar: 100 μm. (B) The hNSCs were positive for neural stem cell markers. Representative images of hNSCs showing PAX6 in purple, Nestin in purple, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (C) Analysis of <t>NSC</t> multipotentiality via a standard differentiation assay showing upregulation of neuronal and neuroglial markers. Representative images of hNSCs showing βIII-tubulin (neuron) in white, GFAP (neuroglia) in green, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (D) Nanoparticle tracking analysis showing the diameters of the hNSC-EVs. (E) TEM showing hNSC-EV morphology. Scale bar: 100 nm. (F) Immunoblot for CD9, CD63, TSG101, and calnexin expression by hNSC-EVs. (G) hNSC-EV internalization by HUVECs, mouse hippocampal neuronal cells (HT22), and mouse microglial cells (BV2). Representative confocal microscope images of cells with green-labeled cytoplasmic and DAPI-stained nuclei (blue) 6 hours after exposure to PKH26-labeled hNSC-EVs (red). Scale bar: 5 μm. All experiments were performed at least three times. DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; hNSC: human neural stem cell; hNSC-EVs: hNSC-derived extracellular vesicles; HUVEC: human umbilical vein endothelial cells; NTA: nanoparticle tracking analysis.
Xf Dmem Base Medium, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xf dmem base medium/product/Agilent technologies
Average 86 stars, based on 1 article reviews
xf dmem base medium - by Bioz Stars, 2025-07
86/100 stars
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seahorse xf base medium  (Agilent technologies)
86
Agilent technologies seahorse xf base medium
Characterization <t>of</t> <t>hNSCs</t> and hNSC-EVs. (A) Morphology of first- and third-passage hNSCs. Scale bar: 100 μm. (B) The hNSCs were positive for neural stem cell markers. Representative images of hNSCs showing PAX6 in purple, Nestin in purple, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (C) Analysis of <t>NSC</t> multipotentiality via a standard differentiation assay showing upregulation of neuronal and neuroglial markers. Representative images of hNSCs showing βIII-tubulin (neuron) in white, GFAP (neuroglia) in green, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (D) Nanoparticle tracking analysis showing the diameters of the hNSC-EVs. (E) TEM showing hNSC-EV morphology. Scale bar: 100 nm. (F) Immunoblot for CD9, CD63, TSG101, and calnexin expression by hNSC-EVs. (G) hNSC-EV internalization by HUVECs, mouse hippocampal neuronal cells (HT22), and mouse microglial cells (BV2). Representative confocal microscope images of cells with green-labeled cytoplasmic and DAPI-stained nuclei (blue) 6 hours after exposure to PKH26-labeled hNSC-EVs (red). Scale bar: 5 μm. All experiments were performed at least three times. DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; hNSC: human neural stem cell; hNSC-EVs: hNSC-derived extracellular vesicles; HUVEC: human umbilical vein endothelial cells; NTA: nanoparticle tracking analysis.
Seahorse Xf Base Medium, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/seahorse xf base medium/product/Agilent technologies
Average 86 stars, based on 1 article reviews
seahorse xf base medium - by Bioz Stars, 2025-07
86/100 stars
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xf base medium  (Agilent technologies)
86
Agilent technologies xf base medium
Characterization <t>of</t> <t>hNSCs</t> and hNSC-EVs. (A) Morphology of first- and third-passage hNSCs. Scale bar: 100 μm. (B) The hNSCs were positive for neural stem cell markers. Representative images of hNSCs showing PAX6 in purple, Nestin in purple, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (C) Analysis of <t>NSC</t> multipotentiality via a standard differentiation assay showing upregulation of neuronal and neuroglial markers. Representative images of hNSCs showing βIII-tubulin (neuron) in white, GFAP (neuroglia) in green, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (D) Nanoparticle tracking analysis showing the diameters of the hNSC-EVs. (E) TEM showing hNSC-EV morphology. Scale bar: 100 nm. (F) Immunoblot for CD9, CD63, TSG101, and calnexin expression by hNSC-EVs. (G) hNSC-EV internalization by HUVECs, mouse hippocampal neuronal cells (HT22), and mouse microglial cells (BV2). Representative confocal microscope images of cells with green-labeled cytoplasmic and DAPI-stained nuclei (blue) 6 hours after exposure to PKH26-labeled hNSC-EVs (red). Scale bar: 5 μm. All experiments were performed at least three times. DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; hNSC: human neural stem cell; hNSC-EVs: hNSC-derived extracellular vesicles; HUVEC: human umbilical vein endothelial cells; NTA: nanoparticle tracking analysis.
Xf Base Medium, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xf base medium/product/Agilent technologies
Average 86 stars, based on 1 article reviews
xf base medium - by Bioz Stars, 2025-07
86/100 stars
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base dmem medium  (Agilent technologies)
86
Agilent technologies base dmem medium
Characterization <t>of</t> <t>hNSCs</t> and hNSC-EVs. (A) Morphology of first- and third-passage hNSCs. Scale bar: 100 μm. (B) The hNSCs were positive for neural stem cell markers. Representative images of hNSCs showing PAX6 in purple, Nestin in purple, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (C) Analysis of <t>NSC</t> multipotentiality via a standard differentiation assay showing upregulation of neuronal and neuroglial markers. Representative images of hNSCs showing βIII-tubulin (neuron) in white, GFAP (neuroglia) in green, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (D) Nanoparticle tracking analysis showing the diameters of the hNSC-EVs. (E) TEM showing hNSC-EV morphology. Scale bar: 100 nm. (F) Immunoblot for CD9, CD63, TSG101, and calnexin expression by hNSC-EVs. (G) hNSC-EV internalization by HUVECs, mouse hippocampal neuronal cells (HT22), and mouse microglial cells (BV2). Representative confocal microscope images of cells with green-labeled cytoplasmic and DAPI-stained nuclei (blue) 6 hours after exposure to PKH26-labeled hNSC-EVs (red). Scale bar: 5 μm. All experiments were performed at least three times. DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; hNSC: human neural stem cell; hNSC-EVs: hNSC-derived extracellular vesicles; HUVEC: human umbilical vein endothelial cells; NTA: nanoparticle tracking analysis.
Base Dmem Medium, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/base dmem medium/product/Agilent technologies
Average 86 stars, based on 1 article reviews
base dmem medium - by Bioz Stars, 2025-07
86/100 stars
  Buy from Supplier
stempro 34 based medium  (ReproCELL)
86
ReproCELL stempro 34 based medium
Characterization <t>of</t> <t>hNSCs</t> and hNSC-EVs. (A) Morphology of first- and third-passage hNSCs. Scale bar: 100 μm. (B) The hNSCs were positive for neural stem cell markers. Representative images of hNSCs showing PAX6 in purple, Nestin in purple, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (C) Analysis of <t>NSC</t> multipotentiality via a standard differentiation assay showing upregulation of neuronal and neuroglial markers. Representative images of hNSCs showing βIII-tubulin (neuron) in white, GFAP (neuroglia) in green, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (D) Nanoparticle tracking analysis showing the diameters of the hNSC-EVs. (E) TEM showing hNSC-EV morphology. Scale bar: 100 nm. (F) Immunoblot for CD9, CD63, TSG101, and calnexin expression by hNSC-EVs. (G) hNSC-EV internalization by HUVECs, mouse hippocampal neuronal cells (HT22), and mouse microglial cells (BV2). Representative confocal microscope images of cells with green-labeled cytoplasmic and DAPI-stained nuclei (blue) 6 hours after exposure to PKH26-labeled hNSC-EVs (red). Scale bar: 5 μm. All experiments were performed at least three times. DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; hNSC: human neural stem cell; hNSC-EVs: hNSC-derived extracellular vesicles; HUVEC: human umbilical vein endothelial cells; NTA: nanoparticle tracking analysis.
Stempro 34 Based Medium, supplied by ReproCELL, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stempro 34 based medium/product/ReproCELL
Average 86 stars, based on 1 article reviews
stempro 34 based medium - by Bioz Stars, 2025-07
86/100 stars
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Characterization of hNSCs and hNSC-EVs. (A) Morphology of first- and third-passage hNSCs. Scale bar: 100 μm. (B) The hNSCs were positive for neural stem cell markers. Representative images of hNSCs showing PAX6 in purple, Nestin in purple, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (C) Analysis of NSC multipotentiality via a standard differentiation assay showing upregulation of neuronal and neuroglial markers. Representative images of hNSCs showing βIII-tubulin (neuron) in white, GFAP (neuroglia) in green, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (D) Nanoparticle tracking analysis showing the diameters of the hNSC-EVs. (E) TEM showing hNSC-EV morphology. Scale bar: 100 nm. (F) Immunoblot for CD9, CD63, TSG101, and calnexin expression by hNSC-EVs. (G) hNSC-EV internalization by HUVECs, mouse hippocampal neuronal cells (HT22), and mouse microglial cells (BV2). Representative confocal microscope images of cells with green-labeled cytoplasmic and DAPI-stained nuclei (blue) 6 hours after exposure to PKH26-labeled hNSC-EVs (red). Scale bar: 5 μm. All experiments were performed at least three times. DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; hNSC: human neural stem cell; hNSC-EVs: hNSC-derived extracellular vesicles; HUVEC: human umbilical vein endothelial cells; NTA: nanoparticle tracking analysis.

Journal: Neural Regeneration Research

Article Title: Human neural stem cell–derived extracellular vesicles protect against ischemic stroke by activating the PI3K/AKT/mTOR pathway

doi: 10.4103/NRR.NRR-D-23-01144

Figure Lengend Snippet: Characterization of hNSCs and hNSC-EVs. (A) Morphology of first- and third-passage hNSCs. Scale bar: 100 μm. (B) The hNSCs were positive for neural stem cell markers. Representative images of hNSCs showing PAX6 in purple, Nestin in purple, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (C) Analysis of NSC multipotentiality via a standard differentiation assay showing upregulation of neuronal and neuroglial markers. Representative images of hNSCs showing βIII-tubulin (neuron) in white, GFAP (neuroglia) in green, and DAPI (nuclear counterstain) in blue. Scale bar: 50 μm. (D) Nanoparticle tracking analysis showing the diameters of the hNSC-EVs. (E) TEM showing hNSC-EV morphology. Scale bar: 100 nm. (F) Immunoblot for CD9, CD63, TSG101, and calnexin expression by hNSC-EVs. (G) hNSC-EV internalization by HUVECs, mouse hippocampal neuronal cells (HT22), and mouse microglial cells (BV2). Representative confocal microscope images of cells with green-labeled cytoplasmic and DAPI-stained nuclei (blue) 6 hours after exposure to PKH26-labeled hNSC-EVs (red). Scale bar: 5 μm. All experiments were performed at least three times. DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; hNSC: human neural stem cell; hNSC-EVs: hNSC-derived extracellular vesicles; HUVEC: human umbilical vein endothelial cells; NTA: nanoparticle tracking analysis.

Article Snippet: The procedure was performed in accordance with GMP level. hNSCs were cultured in NSC complete culture medium composed of NSC base medium (Cat# 05751, Stemcell Technologies, Vancouver, Canada), 1% penicillin and streptomycin (Cat# P1400, Solarbio, Beijing, China), heparin basic fibroblast growth factor (Cat# 07980, Stemcell Technologies), and EGF (Cat# 78136.1, Stemcell Technologies), bFGF (Cat# 78134.1, Stemcell Technologies).

Techniques: Differentiation Assay, Western Blot, Expressing, Microscopy, Labeling, Staining, Derivative Assay