barcode oligonucleotides  (Thermo Fisher)


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    Structured Review

    Thermo Fisher barcode oligonucleotides
    Performance of NGS Technology. ( a ) Bioinformatic pipeline for analysis of individual reads acquired from multiplex sequencing on an Ion Torrent 318 chip. ( b ) Number of reads assigned to each <t>barcode</t> in control DNA mixtures. ( c ) Number of reads assigned to each barcode in field-collected parasites. Coverage map across amplicon for pf-csp ( d ), pf-ama1 ( e ), and pf-k13 ( f , g ). False discovery rates at varying minor allele frequencies for reads from each gene target.
    Barcode Oligonucleotides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/barcode oligonucleotides/product/Thermo Fisher
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    barcode oligonucleotides - by Bioz Stars, 2020-08
    91/100 stars

    Images

    1) Product Images from "Overlap Extension Barcoding for the Next Generation Sequencing and Genotyping of Plasmodium falciparum in Individual Patients in Western Kenya"

    Article Title: Overlap Extension Barcoding for the Next Generation Sequencing and Genotyping of Plasmodium falciparum in Individual Patients in Western Kenya

    Journal: Scientific Reports

    doi: 10.1038/srep41108

    Performance of NGS Technology. ( a ) Bioinformatic pipeline for analysis of individual reads acquired from multiplex sequencing on an Ion Torrent 318 chip. ( b ) Number of reads assigned to each barcode in control DNA mixtures. ( c ) Number of reads assigned to each barcode in field-collected parasites. Coverage map across amplicon for pf-csp ( d ), pf-ama1 ( e ), and pf-k13 ( f , g ). False discovery rates at varying minor allele frequencies for reads from each gene target.
    Figure Legend Snippet: Performance of NGS Technology. ( a ) Bioinformatic pipeline for analysis of individual reads acquired from multiplex sequencing on an Ion Torrent 318 chip. ( b ) Number of reads assigned to each barcode in control DNA mixtures. ( c ) Number of reads assigned to each barcode in field-collected parasites. Coverage map across amplicon for pf-csp ( d ), pf-ama1 ( e ), and pf-k13 ( f , g ). False discovery rates at varying minor allele frequencies for reads from each gene target.

    Techniques Used: Next-Generation Sequencing, Multiplex Assay, Sequencing, Chromatin Immunoprecipitation, Amplification

    ( a ) Workflow for preparation of individually identifiable sequence data from dried blood spots. ( b ) PCR Schema to append unique barcode oligonucleotides to multiplexed gene-target amplicons.
    Figure Legend Snippet: ( a ) Workflow for preparation of individually identifiable sequence data from dried blood spots. ( b ) PCR Schema to append unique barcode oligonucleotides to multiplexed gene-target amplicons.

    Techniques Used: Sequencing, Polymerase Chain Reaction

    Haplotypes present in clinical infection samples. ( a ) Number of unique pf-csp haplotypes contained in field-collected sample DNA by individual, sorted by barcode number. ( b ) Number of individuals bearing each pf-csp haplotype. ( c ) Distribution of pf-csp haplotypes among field-collected sample DNA. Each color indicates a different haplotype. ( d ) MOI values segregated based upon symptomaticity. ( e ) MOI values segregated based upon sampling date.
    Figure Legend Snippet: Haplotypes present in clinical infection samples. ( a ) Number of unique pf-csp haplotypes contained in field-collected sample DNA by individual, sorted by barcode number. ( b ) Number of individuals bearing each pf-csp haplotype. ( c ) Distribution of pf-csp haplotypes among field-collected sample DNA. Each color indicates a different haplotype. ( d ) MOI values segregated based upon symptomaticity. ( e ) MOI values segregated based upon sampling date.

    Techniques Used: Infection, Sampling

    2) Product Images from "Overlap Extension Barcoding for the Next Generation Sequencing and Genotyping of Plasmodium falciparum in Individual Patients in Western Kenya"

    Article Title: Overlap Extension Barcoding for the Next Generation Sequencing and Genotyping of Plasmodium falciparum in Individual Patients in Western Kenya

    Journal: Scientific Reports

    doi: 10.1038/srep41108

    Performance of NGS Technology. ( a ) Bioinformatic pipeline for analysis of individual reads acquired from multiplex sequencing on an Ion Torrent 318 chip. ( b ) Number of reads assigned to each barcode in control DNA mixtures. ( c ) Number of reads assigned to each barcode in field-collected parasites. Coverage map across amplicon for pf-csp ( d ), pf-ama1 ( e ), and pf-k13 ( f , g ). False discovery rates at varying minor allele frequencies for reads from each gene target.
    Figure Legend Snippet: Performance of NGS Technology. ( a ) Bioinformatic pipeline for analysis of individual reads acquired from multiplex sequencing on an Ion Torrent 318 chip. ( b ) Number of reads assigned to each barcode in control DNA mixtures. ( c ) Number of reads assigned to each barcode in field-collected parasites. Coverage map across amplicon for pf-csp ( d ), pf-ama1 ( e ), and pf-k13 ( f , g ). False discovery rates at varying minor allele frequencies for reads from each gene target.

    Techniques Used: Next-Generation Sequencing, Multiplex Assay, Sequencing, Chromatin Immunoprecipitation, Amplification

    ( a ) Workflow for preparation of individually identifiable sequence data from dried blood spots. ( b ) PCR Schema to append unique barcode oligonucleotides to multiplexed gene-target amplicons.
    Figure Legend Snippet: ( a ) Workflow for preparation of individually identifiable sequence data from dried blood spots. ( b ) PCR Schema to append unique barcode oligonucleotides to multiplexed gene-target amplicons.

    Techniques Used: Sequencing, Polymerase Chain Reaction

    Haplotypes present in clinical infection samples. ( a ) Number of unique pf-csp haplotypes contained in field-collected sample DNA by individual, sorted by barcode number. ( b ) Number of individuals bearing each pf-csp haplotype. ( c ) Distribution of pf-csp haplotypes among field-collected sample DNA. Each color indicates a different haplotype. ( d ) MOI values segregated based upon symptomaticity. ( e ) MOI values segregated based upon sampling date.
    Figure Legend Snippet: Haplotypes present in clinical infection samples. ( a ) Number of unique pf-csp haplotypes contained in field-collected sample DNA by individual, sorted by barcode number. ( b ) Number of individuals bearing each pf-csp haplotype. ( c ) Distribution of pf-csp haplotypes among field-collected sample DNA. Each color indicates a different haplotype. ( d ) MOI values segregated based upon symptomaticity. ( e ) MOI values segregated based upon sampling date.

    Techniques Used: Infection, Sampling

    Related Articles

    Polymerase Chain Reaction:

    Article Title: DUX4 regulates oocyte to embryo transition in human
    Article Snippet: .. After addition of the barcode oligos the DNA samples were amplified for 12 cycles (98°C for 10 s, 63°C for 30 s and 72°C for 60 s) in Phusion PCR master mix (Thermo Fisher Scientific). ..

    Sequencing:

    Article Title: Coupling Bacterial Community Assembly to Microbial Metabolism across Soil Profiles
    Article Snippet: .. Both the forward and reverse primers were tagged with an adapter and linker sequence, and barcode oligonucleotides that were 8 bp in length were added to the reverse primer to distinguish the 16S rRNA amplicons that originated from different soil samples. ..

    Generated:

    Article Title: Overlap Extension Barcoding for the Next Generation Sequencing and Genotyping of Plasmodium falciparum in Individual Patients in Western Kenya
    Article Snippet: .. First, we generated “barcode oligonucleotides” by Taq extension of primers containing the IonTorrent Adaptor A and each barcode followed by a linker ( Step 1A). .. This reaction consisted of 200 nM barcode primers, 200 nM IonTorrent Adaptor A forward adapter, 1X Taq buffer, 200 μM deoxynucleotides, 1.5 mM magnesium chloride and 0.5 units of Invitrogen platinum Taq (Life Technologies, BioRad); this reaction was incubated for 15 minutes at 95C, and then underwent 25 cycles of 95C for 30 s, 60C for 30 s, and 72C for 1 m, followed by 5 m at 72C.

    Amplification:

    Article Title: DUX4 regulates oocyte to embryo transition in human
    Article Snippet: .. After addition of the barcode oligos the DNA samples were amplified for 12 cycles (98°C for 10 s, 63°C for 30 s and 72°C for 60 s) in Phusion PCR master mix (Thermo Fisher Scientific). ..

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