banana lectin banlec  (Vector Laboratories)

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Influenza C virus susceptibility to antivirals with different mechanisms of action

doi: 10.1128/aac.01727-23

Figure Lengend Snippet: Antiviral activity against influenza C viruses assessed by HINT in MDCK cells a

Article Snippet: Given the recent interest in developing engineered lectins as antivirals ( 16 ), we also tested type C viruses with banana lectin (BanLec) (L-1410, Vector Laboratories), which can bind to surface glycoproteins of influenza viruses ( 17 ).

Techniques: Activity Assay

Journal: The Journal of Biological Chemistry

Article Title: The barley lectin, horcolin, binds high-mannose glycans in a multivalent fashion, enabling high-affinity, specific inhibition of cellular HIV infection

doi: 10.1074/jbc.RA120.013100

Figure Lengend Snippet: Thermodynamics of horcolin-glycan interactions. A, structures of the various manno-oligosaccharides used in the study. B, schematic representation of the HR complexed with Man3 and Man7D1. Shown are results of a typical isothermal titration calorimetry experiment. Top, exothermic microcalorimetric traces of Man7-D1 injections into horcolin solution (0.8 mm). Bottom, Wiseman plot of heat releases versus molar ratio of injectant/protein in the cell and a nonlinear fit of the binding isotherm for equivalent binding site(s). C, thermodynamic profile indicating binding free energy, enthalpy, and entropy for the 11-HR mannooligosaccharide interaction. D, enthalpy-entropy compensation plot of glycan binding to horcolin at 25 °C. Enthalpy and entropy values are from Table 2. Straight line, best least-squares fit of the data.

Article Snippet: Abbreviations —The abbreviations used are: HR horcolin CBS carbohydrate-binding site MD molecular dynamics EDC 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide NHS N -hydroxysuccinimide X-Gal 5-bromo-4-chloro-3-indolyl β-d-galactoside Man mannose mJRL mannose-specific jacalin-related lectin ITC isothermal titration calorimetry BanLec banana lectin(s) MSA multiple-sequence alignment SEC-MALS size-exclusion chromatography coupled to multiangle light scattering Me-α-Man methyl-α-mannopyranoside Ni-NTA nickel-nitrilotriacetic acid PDB Protein Data Bank RMSD root mean square deviation RMSF root mean square fluctuation RU response units GNA Galanthus nivalis agglutinin GRFT griffithsin IL interleukin ConA concanavalin A MM/PBSA molecular mechanics/Poisson–Boltzmann surface area SPR surface plasmon resonance DMEM Dulbecco's modified Eagle's medium gp120 glycoprotein 120 gp140 glycoprotein 140 BLAST Basic Local Alignment Search Tool GROMACS Groningen Machine for Chemical Simulations.

Techniques: Isothermal Titration Calorimetry, Binding Assay