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OriGene bamhi
Bamhi, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi/product/OriGene
Average 92 stars, based on 76 article reviews
Price from $9.99 to $1999.99
bamhi - by Bioz Stars, 2020-10
92/100 stars

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Related Articles

Clone Assay:

Article Title: Lipid transport by TMEM24 at ER–plasma membrane contacts regulates pulsatile insulin secretion
Article Snippet: .. TMEM24 fragments amplified by PCR were cloned into pCDF-C6P using EcoRI or BamHI and NotI sites for bacterial expression with an N-terminal GST tag. pCMV6-AN-His (Origene) was modified to include an N-terminal His12 tag. .. TMEM24 ΔTM (36–706) was cloned using XhoI and NotI sites into pCMV6-AN-12His for Expi293 cell expression.

Article Title: Rab22A recruits BLOC‐1 and BLOC‐2 to promote the biogenesis of recycling endosomes
Article Snippet: .. Retroviral shRNA vectors Oligodeoxyribonucleotide duplexes containing the target sequences were cloned into the BamHI and HindIII sites of pRS shRNA vector (OriGene Technologies). ..

Article Title: miRNA-mediated post-transcriptional silencing of transgenes leads to increased adeno-associated viral vector yield and targeting specificity.
Article Snippet: .. The production of high-titer recombinant adeno-associated virus (rAAV) vector is essential for treatment of genetic diseases affecting the retina and choroid, where anatomical constraints may limit injectable volumes. .. The production of high-titer recombinant adeno-associated virus (rAAV) vector is essential for treatment of genetic diseases affecting the retina and choroid, where anatomical constraints may limit injectable volumes.

Amplification:

Article Title: Lipid transport by TMEM24 at ER–plasma membrane contacts regulates pulsatile insulin secretion
Article Snippet: .. TMEM24 fragments amplified by PCR were cloned into pCDF-C6P using EcoRI or BamHI and NotI sites for bacterial expression with an N-terminal GST tag. pCMV6-AN-His (Origene) was modified to include an N-terminal His12 tag. .. TMEM24 ΔTM (36–706) was cloned using XhoI and NotI sites into pCMV6-AN-12His for Expi293 cell expression.

Article Title: miRNA-mediated post-transcriptional silencing of transgenes leads to increased adeno-associated viral vector yield and targeting specificity.
Article Snippet: .. The production of high-titer recombinant adeno-associated virus (rAAV) vector is essential for treatment of genetic diseases affecting the retina and choroid, where anatomical constraints may limit injectable volumes. .. The production of high-titer recombinant adeno-associated virus (rAAV) vector is essential for treatment of genetic diseases affecting the retina and choroid, where anatomical constraints may limit injectable volumes.

Polymerase Chain Reaction:

Article Title: Lipid transport by TMEM24 at ER–plasma membrane contacts regulates pulsatile insulin secretion
Article Snippet: .. TMEM24 fragments amplified by PCR were cloned into pCDF-C6P using EcoRI or BamHI and NotI sites for bacterial expression with an N-terminal GST tag. pCMV6-AN-His (Origene) was modified to include an N-terminal His12 tag. .. TMEM24 ΔTM (36–706) was cloned using XhoI and NotI sites into pCMV6-AN-12His for Expi293 cell expression.

shRNA:

Article Title: Rab22A recruits BLOC‐1 and BLOC‐2 to promote the biogenesis of recycling endosomes
Article Snippet: .. Retroviral shRNA vectors Oligodeoxyribonucleotide duplexes containing the target sequences were cloned into the BamHI and HindIII sites of pRS shRNA vector (OriGene Technologies). ..

Expressing:

Article Title: Lipid transport by TMEM24 at ER–plasma membrane contacts regulates pulsatile insulin secretion
Article Snippet: .. TMEM24 fragments amplified by PCR were cloned into pCDF-C6P using EcoRI or BamHI and NotI sites for bacterial expression with an N-terminal GST tag. pCMV6-AN-His (Origene) was modified to include an N-terminal His12 tag. .. TMEM24 ΔTM (36–706) was cloned using XhoI and NotI sites into pCMV6-AN-12His for Expi293 cell expression.

Article Title: miRNA-mediated post-transcriptional silencing of transgenes leads to increased adeno-associated viral vector yield and targeting specificity.
Article Snippet: .. The production of high-titer recombinant adeno-associated virus (rAAV) vector is essential for treatment of genetic diseases affecting the retina and choroid, where anatomical constraints may limit injectable volumes. .. The production of high-titer recombinant adeno-associated virus (rAAV) vector is essential for treatment of genetic diseases affecting the retina and choroid, where anatomical constraints may limit injectable volumes.

Modification:

Article Title: Lipid transport by TMEM24 at ER–plasma membrane contacts regulates pulsatile insulin secretion
Article Snippet: .. TMEM24 fragments amplified by PCR were cloned into pCDF-C6P using EcoRI or BamHI and NotI sites for bacterial expression with an N-terminal GST tag. pCMV6-AN-His (Origene) was modified to include an N-terminal His12 tag. .. TMEM24 ΔTM (36–706) was cloned using XhoI and NotI sites into pCMV6-AN-12His for Expi293 cell expression.

Plasmid Preparation:

Article Title: An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching
Article Snippet: .. Thus, to prepare mCerulean3 N-terminal fusions, the following digests were performed: human non-muscle α-actinin, EcoRI and NotI (vector source, Tom Keller, FSU); human cytochrome C oxidase subunit VIII, BamHI and NotI (mitochondria, Clontech); human zyxin, BamHI and NotI (Clare Waterman-Storer, NIH); rat α-1 connexin-43, EcoRI and BamHI (Matthias Falk, Lehigh University); human H2B, BamHI and NotI (George Patterson, NIH); N-terminal 81 aa of human β-1,4-galactosyltransferase, BamHI and NotI (Golgi, Clontech); human microtubule-associated protein EB3, BamHI and NotI (Lynne Cassimeris, Lehigh University); human vimentin, BamHI and NotI (Robert Goldman, Northwestern University); human peroxisomal membrane protein 2, NotI and AgeI (peroxisomes; OriGene); c-src, BamHI and NotI (chicken c-src tyrosine kinase, Marilyn Resh, Sloan-Kettering Institute); lifeact, BamHI and NotI (N-terminal 17 aa from S. cerevisiae Abp 140, IDT); VE-cadherin, BamHI and NotI (human vascular epithelial cadherin, Andreea Trache, Texas A & M); fascin, BamHI and NotI (human fascin, OriGene). .. To prepare mCerulean3 C-terminal fusions, the following digests were performed: human β-actin, NheI and BglII (Clontech); human α-tubulin, NheI and BglII (Clontech); human light chain clathrin, NheI and BglII (George Patterson, NIH); human lamin B1, NheI and BglII (George Patterson, NIH); mouse MAP4, NheI and BglII (mouse microtubule associated protein 4, nucleotides 1918–3135, Richard Cyr, Penn State University); mouse light chain 9 myosin, NheI and BglII (Patricia Wadsworth, University of Massachusetts); human CDC-42, NheI and BglII (OriGene); PCNA, AgeI and BspEI (David Gilbert, FSU); mouse CAF-1, NheI and BglII (Akash Gunjan, FSU); human fibrillarin, AgeI and BspEI (Dimitry Chudakov, Russian Academy of Sciences); human GTPase Rab5a, NheI and BglII (Vicky Allen, University of Manchester).

Article Title: Rab22A recruits BLOC‐1 and BLOC‐2 to promote the biogenesis of recycling endosomes
Article Snippet: .. Retroviral shRNA vectors Oligodeoxyribonucleotide duplexes containing the target sequences were cloned into the BamHI and HindIII sites of pRS shRNA vector (OriGene Technologies). ..

Article Title: miRNA-mediated post-transcriptional silencing of transgenes leads to increased adeno-associated viral vector yield and targeting specificity.
Article Snippet: .. The production of high-titer recombinant adeno-associated virus (rAAV) vector is essential for treatment of genetic diseases affecting the retina and choroid, where anatomical constraints may limit injectable volumes. .. The production of high-titer recombinant adeno-associated virus (rAAV) vector is essential for treatment of genetic diseases affecting the retina and choroid, where anatomical constraints may limit injectable volumes.

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  • 84
    thermo fisher r fix 293 cells a bamhi fragment
    Restoration of carboxylase activity in edited <t>fIX</t> 293 cells. (A-B) Carboxylase expression and activity were monitored in edited fIX 293 cells stably-transfected with r-carboxylase flag .
    R Fix 293 Cells A Bamhi Fragment, supplied by thermo fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r fix 293 cells a bamhi fragment/product/thermo fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    r fix 293 cells a bamhi fragment - by Bioz Stars, 2020-10
    84/100 stars
      Buy from Supplier

    92
    OriGene bamhi
    Restoration of carboxylase activity in edited <t>fIX</t> 293 cells. (A-B) Carboxylase expression and activity were monitored in edited fIX 293 cells stably-transfected with r-carboxylase flag .
    Bamhi, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi/product/OriGene
    Average 92 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    bamhi - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    92
    OriGene bamhi sacii fragment
    Restoration of carboxylase activity in edited <t>fIX</t> 293 cells. (A-B) Carboxylase expression and activity were monitored in edited fIX 293 cells stably-transfected with r-carboxylase flag .
    Bamhi Sacii Fragment, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi sacii fragment/product/OriGene
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bamhi sacii fragment - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    Image Search Results


    Restoration of carboxylase activity in edited fIX 293 cells. (A-B) Carboxylase expression and activity were monitored in edited fIX 293 cells stably-transfected with r-carboxylase flag .

    Journal: Journal of thrombosis and haemostasis : JTH

    Article Title: Exon 2 Skipping Eliminates Gamma-Glutamyl Carboxylase Activity, Indicating a Partial Splicing Defect in a Patient with Vitamin K Clotting Factor Deficiency

    doi: 10.1111/jth.14456

    Figure Lengend Snippet: Restoration of carboxylase activity in edited fIX 293 cells. (A-B) Carboxylase expression and activity were monitored in edited fIX 293 cells stably-transfected with r-carboxylase flag .

    Article Snippet: Generation of r-fIX 293 cells.A BamHI fragment containing the fIX cDNA was subcloned into pCMV6-A-puro (Origene), and the plasmid was transfected into 293 cells.

    Techniques: Activity Assay, Expressing, Stable Transfection, Transfection

    Δ2GGCX is inactive for fIX carboxylation in 293 cells. (A) Edited fIX 293 cells expressing wild type (r-WT flag ) or exon 2-deleted (r-Δ2GGCX flag .

    Journal: Journal of thrombosis and haemostasis : JTH

    Article Title: Exon 2 Skipping Eliminates Gamma-Glutamyl Carboxylase Activity, Indicating a Partial Splicing Defect in a Patient with Vitamin K Clotting Factor Deficiency

    doi: 10.1111/jth.14456

    Figure Lengend Snippet: Δ2GGCX is inactive for fIX carboxylation in 293 cells. (A) Edited fIX 293 cells expressing wild type (r-WT flag ) or exon 2-deleted (r-Δ2GGCX flag .

    Article Snippet: Generation of r-fIX 293 cells.A BamHI fragment containing the fIX cDNA was subcloned into pCMV6-A-puro (Origene), and the plasmid was transfected into 293 cells.

    Techniques: Expressing