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GE Healthcare bamhi xhoi sites
Bamhi Xhoi Sites, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi xhoi sites/product/GE Healthcare
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
bamhi xhoi sites - by Bioz Stars, 2020-07
91/100 stars

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Clone Assay:

Article Title: Capsomer Vaccines Protect Mice from Vaginal Challenge with Human Papillomavirus
Article Snippet: .. It was then cloned into the BamHI/XhoI sites of pGEX-4T-2 (GE Healthcare) and verified by sequencing. .. Plasmid pGEX-HPV16 L1Δ-L2x3 was constructed by amplifying HPV16 L1ΔN9ΔC29 from the aforementioned plasmid and reinserting the L1 segment into pGEX-4T-2 using the BamHI and EcoRI restriction sites.

Article Title: Two C-type Lectins Cooperate to Defend Anopheles gambiae against Gram-negative Bacteria *
Article Snippet: .. Amplicons were subcloned into pGEM-T easy vector (Promega), sequenced, and then cloned into BamHI/XhoI sites of pGEX-4T-3 expression vector (GE Healthcare). ..

Article Title: Roles of Inflammatory Caspases during Processing of Zebrafish Interleukin-1? in Francisella noatunensis Infection
Article Snippet: .. The PCR product was gel purified, cloned into the BamHI/XhoI sites of pGEX-4T-1 (GE Healthcare), and confirmed by sequencing. .. Serum from rabbits immunized with purified GST-tagged zebrafish ( Danio rerio ) IL-1β was obtained from Cocalico Biologicals, Inc. Serum was diluted 1:2 in PBS buffer and passed 3 times over an immobilized GST-agarose column (Thermo) to remove antibodies to GST.

Article Title: RSUME Enhances Glucocorticoid Receptor SUMOylation and Transcriptional Activity
Article Snippet: .. To generate glutathione S -transferase (GST)-labeled RSUME (GST-RSUME), human RSUME cDNA was amplified by PCR and cloned into the BamHI-XhoI sites of pGEX-4T3 (GE Healthcare). .. The PGEX-4T3 vector was used to express GST.

Article Title: Spatiotemporal regulation of the GPCR activity of BAI3 by C1qL4 and Stabilin-2 controls myoblast fusion
Article Snippet: .. To generate a Glutathione S -transferase (GST)-fusion protein of C1qL4, the corresponding coding sequences was amplified by PCR from pDisplay-HA-C1qL4 (kind gift of Dr. T. Südhof (Stanford University)) and cloned into BamHI/XhoI sites of the pGEX4T1 plasmid (GE Healthcare Life Sciences). .. The pcDNA5-V5-Stabilin-2 plasmid was a kind gift from Dr. Harris and pcDNA3-HA-C1qL1, 2, and 3 plasmids were a kind gift from Dr. Wong were previously described .

Amplification:

Article Title: Nrf2- and ATF4-Dependent Upregulation of xCT Modulates the Sensitivity of T24 Bladder Carcinoma Cells to Proteasome Inhibition
Article Snippet: .. The amplified Nrf2 cDNA fragment was digested with BamHI and XhoI and subcloned into the BamHI/XhoI sites of pGEX-5×-1 vector (GE Healthcare). .. To construct pGEX-hNrf2 (1–209), pGEX-hNrf2 (210–419), and pGEX-hNrf2 (420–605), corresponding human Nrf2 cDNA fragments were PCR amplified by using the following primer sets: 5′-GC G GAT CC C TCA TCA TGA TGG ACT TGG AGC-3′ and 5′-GGG CTC GAG CTC AAC CAG CTT GTC ATT TTC AAT ATT A-3′, 5′-GC G GAT CC A CTA CCA TGG TTC CAA GTC CAG AA-3′ and 5′-GGG CTC GAG ATC ATG CAC GTG AGT GCT CTG C-3′, and 5′-GC G GAT CC A CCA TGG CCC AAT GTG AGA ACA CAC CA-3′ and 5′-GGG CTC GAG GTC AAA TCC TCC TAA ATC TAG TTT-3′, respectively.

Article Title: RSUME Enhances Glucocorticoid Receptor SUMOylation and Transcriptional Activity
Article Snippet: .. To generate glutathione S -transferase (GST)-labeled RSUME (GST-RSUME), human RSUME cDNA was amplified by PCR and cloned into the BamHI-XhoI sites of pGEX-4T3 (GE Healthcare). .. The PGEX-4T3 vector was used to express GST.

Article Title: Lycopene Inhibits Activation of Epidermal Growth Factor Receptor and Expression of Cyclooxygenase-2 in Gastric Cancer Cells
Article Snippet: .. The cDNA encoding the Ras binding domain (RBD) of Raf1 was amplified by PCR and then subcloned into the pGEX-4T-1 vector at the BamHI-XhoI sites (Amersham Biosciences, Piscataway, NJ, USA). .. The resulting plasmid was used to transform BL23 Escherichia coli cells.

Article Title: Spatiotemporal regulation of the GPCR activity of BAI3 by C1qL4 and Stabilin-2 controls myoblast fusion
Article Snippet: .. To generate a Glutathione S -transferase (GST)-fusion protein of C1qL4, the corresponding coding sequences was amplified by PCR from pDisplay-HA-C1qL4 (kind gift of Dr. T. Südhof (Stanford University)) and cloned into BamHI/XhoI sites of the pGEX4T1 plasmid (GE Healthcare Life Sciences). .. The pcDNA5-V5-Stabilin-2 plasmid was a kind gift from Dr. Harris and pcDNA3-HA-C1qL1, 2, and 3 plasmids were a kind gift from Dr. Wong were previously described .

Purification:

Article Title: Roles of Inflammatory Caspases during Processing of Zebrafish Interleukin-1? in Francisella noatunensis Infection
Article Snippet: .. The PCR product was gel purified, cloned into the BamHI/XhoI sites of pGEX-4T-1 (GE Healthcare), and confirmed by sequencing. .. Serum from rabbits immunized with purified GST-tagged zebrafish ( Danio rerio ) IL-1β was obtained from Cocalico Biologicals, Inc. Serum was diluted 1:2 in PBS buffer and passed 3 times over an immobilized GST-agarose column (Thermo) to remove antibodies to GST.

Sequencing:

Article Title: Capsomer Vaccines Protect Mice from Vaginal Challenge with Human Papillomavirus
Article Snippet: .. It was then cloned into the BamHI/XhoI sites of pGEX-4T-2 (GE Healthcare) and verified by sequencing. .. Plasmid pGEX-HPV16 L1Δ-L2x3 was constructed by amplifying HPV16 L1ΔN9ΔC29 from the aforementioned plasmid and reinserting the L1 segment into pGEX-4T-2 using the BamHI and EcoRI restriction sites.

Article Title: Purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina
Article Snippet: .. Partial nucleotide sequence corresponding to Ile506—Lys593 in carp calnexin (AB894402.1) was inserted into the BamHI/XhoI sites of an expression vector, pGEX-5X-1 (GE Healthcare, Chicago, USA), to obtain N-terminal Glutathione S-transferase (GST) fusion peptide of calnexin. .. It was expressed in E. coli BL21DE3 (Merck Millipore, Massachusetts, USA) and purified using a glutathione resin column according to the manufacturer's instruction.

Article Title: Roles of Inflammatory Caspases during Processing of Zebrafish Interleukin-1? in Francisella noatunensis Infection
Article Snippet: .. The PCR product was gel purified, cloned into the BamHI/XhoI sites of pGEX-4T-1 (GE Healthcare), and confirmed by sequencing. .. Serum from rabbits immunized with purified GST-tagged zebrafish ( Danio rerio ) IL-1β was obtained from Cocalico Biologicals, Inc. Serum was diluted 1:2 in PBS buffer and passed 3 times over an immobilized GST-agarose column (Thermo) to remove antibodies to GST.

Expressing:

Article Title: Purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina
Article Snippet: .. Partial nucleotide sequence corresponding to Ile506—Lys593 in carp calnexin (AB894402.1) was inserted into the BamHI/XhoI sites of an expression vector, pGEX-5X-1 (GE Healthcare, Chicago, USA), to obtain N-terminal Glutathione S-transferase (GST) fusion peptide of calnexin. .. It was expressed in E. coli BL21DE3 (Merck Millipore, Massachusetts, USA) and purified using a glutathione resin column according to the manufacturer's instruction.

Article Title: Two C-type Lectins Cooperate to Defend Anopheles gambiae against Gram-negative Bacteria *
Article Snippet: .. Amplicons were subcloned into pGEM-T easy vector (Promega), sequenced, and then cloned into BamHI/XhoI sites of pGEX-4T-3 expression vector (GE Healthcare). ..

Polymerase Chain Reaction:

Article Title: Roles of Inflammatory Caspases during Processing of Zebrafish Interleukin-1? in Francisella noatunensis Infection
Article Snippet: .. The PCR product was gel purified, cloned into the BamHI/XhoI sites of pGEX-4T-1 (GE Healthcare), and confirmed by sequencing. .. Serum from rabbits immunized with purified GST-tagged zebrafish ( Danio rerio ) IL-1β was obtained from Cocalico Biologicals, Inc. Serum was diluted 1:2 in PBS buffer and passed 3 times over an immobilized GST-agarose column (Thermo) to remove antibodies to GST.

Article Title: RSUME Enhances Glucocorticoid Receptor SUMOylation and Transcriptional Activity
Article Snippet: .. To generate glutathione S -transferase (GST)-labeled RSUME (GST-RSUME), human RSUME cDNA was amplified by PCR and cloned into the BamHI-XhoI sites of pGEX-4T3 (GE Healthcare). .. The PGEX-4T3 vector was used to express GST.

Article Title: Lycopene Inhibits Activation of Epidermal Growth Factor Receptor and Expression of Cyclooxygenase-2 in Gastric Cancer Cells
Article Snippet: .. The cDNA encoding the Ras binding domain (RBD) of Raf1 was amplified by PCR and then subcloned into the pGEX-4T-1 vector at the BamHI-XhoI sites (Amersham Biosciences, Piscataway, NJ, USA). .. The resulting plasmid was used to transform BL23 Escherichia coli cells.

Article Title: Spatiotemporal regulation of the GPCR activity of BAI3 by C1qL4 and Stabilin-2 controls myoblast fusion
Article Snippet: .. To generate a Glutathione S -transferase (GST)-fusion protein of C1qL4, the corresponding coding sequences was amplified by PCR from pDisplay-HA-C1qL4 (kind gift of Dr. T. Südhof (Stanford University)) and cloned into BamHI/XhoI sites of the pGEX4T1 plasmid (GE Healthcare Life Sciences). .. The pcDNA5-V5-Stabilin-2 plasmid was a kind gift from Dr. Harris and pcDNA3-HA-C1qL1, 2, and 3 plasmids were a kind gift from Dr. Wong were previously described .

Binding Assay:

Article Title: Lycopene Inhibits Activation of Epidermal Growth Factor Receptor and Expression of Cyclooxygenase-2 in Gastric Cancer Cells
Article Snippet: .. The cDNA encoding the Ras binding domain (RBD) of Raf1 was amplified by PCR and then subcloned into the pGEX-4T-1 vector at the BamHI-XhoI sites (Amersham Biosciences, Piscataway, NJ, USA). .. The resulting plasmid was used to transform BL23 Escherichia coli cells.

Plasmid Preparation:

Article Title: Nrf2- and ATF4-Dependent Upregulation of xCT Modulates the Sensitivity of T24 Bladder Carcinoma Cells to Proteasome Inhibition
Article Snippet: .. The amplified Nrf2 cDNA fragment was digested with BamHI and XhoI and subcloned into the BamHI/XhoI sites of pGEX-5×-1 vector (GE Healthcare). .. To construct pGEX-hNrf2 (1–209), pGEX-hNrf2 (210–419), and pGEX-hNrf2 (420–605), corresponding human Nrf2 cDNA fragments were PCR amplified by using the following primer sets: 5′-GC G GAT CC C TCA TCA TGA TGG ACT TGG AGC-3′ and 5′-GGG CTC GAG CTC AAC CAG CTT GTC ATT TTC AAT ATT A-3′, 5′-GC G GAT CC A CTA CCA TGG TTC CAA GTC CAG AA-3′ and 5′-GGG CTC GAG ATC ATG CAC GTG AGT GCT CTG C-3′, and 5′-GC G GAT CC A CCA TGG CCC AAT GTG AGA ACA CAC CA-3′ and 5′-GGG CTC GAG GTC AAA TCC TCC TAA ATC TAG TTT-3′, respectively.

Article Title: Purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina
Article Snippet: .. Partial nucleotide sequence corresponding to Ile506—Lys593 in carp calnexin (AB894402.1) was inserted into the BamHI/XhoI sites of an expression vector, pGEX-5X-1 (GE Healthcare, Chicago, USA), to obtain N-terminal Glutathione S-transferase (GST) fusion peptide of calnexin. .. It was expressed in E. coli BL21DE3 (Merck Millipore, Massachusetts, USA) and purified using a glutathione resin column according to the manufacturer's instruction.

Article Title: Two C-type Lectins Cooperate to Defend Anopheles gambiae against Gram-negative Bacteria *
Article Snippet: .. Amplicons were subcloned into pGEM-T easy vector (Promega), sequenced, and then cloned into BamHI/XhoI sites of pGEX-4T-3 expression vector (GE Healthcare). ..

Article Title: Lycopene Inhibits Activation of Epidermal Growth Factor Receptor and Expression of Cyclooxygenase-2 in Gastric Cancer Cells
Article Snippet: .. The cDNA encoding the Ras binding domain (RBD) of Raf1 was amplified by PCR and then subcloned into the pGEX-4T-1 vector at the BamHI-XhoI sites (Amersham Biosciences, Piscataway, NJ, USA). .. The resulting plasmid was used to transform BL23 Escherichia coli cells.

Article Title: Spatiotemporal regulation of the GPCR activity of BAI3 by C1qL4 and Stabilin-2 controls myoblast fusion
Article Snippet: .. To generate a Glutathione S -transferase (GST)-fusion protein of C1qL4, the corresponding coding sequences was amplified by PCR from pDisplay-HA-C1qL4 (kind gift of Dr. T. Südhof (Stanford University)) and cloned into BamHI/XhoI sites of the pGEX4T1 plasmid (GE Healthcare Life Sciences). .. The pcDNA5-V5-Stabilin-2 plasmid was a kind gift from Dr. Harris and pcDNA3-HA-C1qL1, 2, and 3 plasmids were a kind gift from Dr. Wong were previously described .

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    GE Healthcare bamhi xhoi site
    Bamhi Xhoi Site, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi xhoi site/product/GE Healthcare
    Average 89 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    bamhi xhoi site - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    91
    GE Healthcare bamhi xhoi sites
    Bamhi Xhoi Sites, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi xhoi sites/product/GE Healthcare
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bamhi xhoi sites - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    88
    GE Healthcare bamhi xhoi restriction sites
    Bamhi Xhoi Restriction Sites, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi xhoi restriction sites/product/GE Healthcare
    Average 88 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    bamhi xhoi restriction sites - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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