Structured Review

ATG:biosynthetics bamhi smai sites
Bamhi Smai Sites, supplied by ATG:biosynthetics, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi smai sites/product/ATG:biosynthetics
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
bamhi smai sites - by Bioz Stars, 2020-07
93/100 stars

Images

Related Articles

Clone Assay:

Article Title: Expression and characterization of recombinant Par j 1 and Par j 2 resembling the allergenic epitopes of Parietaria judaica pollen
Article Snippet: .. Cloning and generating of high molecular weight recombinant bacmid DNA Synthetic genes, codon-optimized for expression in insect cells coding for Par j 2.0101 (340 base pairs; accession number P55958) and for Par j 1.0102 (451 base pairs; accession number O04404) both including an additional 3′ sequence coding for a C-terminal hexahisitidine tag (Table ), were subcloned into plasmid pTM1 via the BamHI/SmaI sites (ATG: biosynthetics, Merzhausen, Germany). .. The plasmids were transformed into DH10Bactm E. coli cells to generate high molecular weight recombinant bacmid DNA which was then used to transfect Sf9 insect cells to obtain virus stocks .

Molecular Weight:

Article Title: Expression and characterization of recombinant Par j 1 and Par j 2 resembling the allergenic epitopes of Parietaria judaica pollen
Article Snippet: .. Cloning and generating of high molecular weight recombinant bacmid DNA Synthetic genes, codon-optimized for expression in insect cells coding for Par j 2.0101 (340 base pairs; accession number P55958) and for Par j 1.0102 (451 base pairs; accession number O04404) both including an additional 3′ sequence coding for a C-terminal hexahisitidine tag (Table ), were subcloned into plasmid pTM1 via the BamHI/SmaI sites (ATG: biosynthetics, Merzhausen, Germany). .. The plasmids were transformed into DH10Bactm E. coli cells to generate high molecular weight recombinant bacmid DNA which was then used to transfect Sf9 insect cells to obtain virus stocks .

Expressing:

Article Title: Expression and characterization of recombinant Par j 1 and Par j 2 resembling the allergenic epitopes of Parietaria judaica pollen
Article Snippet: .. Cloning and generating of high molecular weight recombinant bacmid DNA Synthetic genes, codon-optimized for expression in insect cells coding for Par j 2.0101 (340 base pairs; accession number P55958) and for Par j 1.0102 (451 base pairs; accession number O04404) both including an additional 3′ sequence coding for a C-terminal hexahisitidine tag (Table ), were subcloned into plasmid pTM1 via the BamHI/SmaI sites (ATG: biosynthetics, Merzhausen, Germany). .. The plasmids were transformed into DH10Bactm E. coli cells to generate high molecular weight recombinant bacmid DNA which was then used to transfect Sf9 insect cells to obtain virus stocks .

Sequencing:

Article Title: Expression and characterization of recombinant Par j 1 and Par j 2 resembling the allergenic epitopes of Parietaria judaica pollen
Article Snippet: .. Cloning and generating of high molecular weight recombinant bacmid DNA Synthetic genes, codon-optimized for expression in insect cells coding for Par j 2.0101 (340 base pairs; accession number P55958) and for Par j 1.0102 (451 base pairs; accession number O04404) both including an additional 3′ sequence coding for a C-terminal hexahisitidine tag (Table ), were subcloned into plasmid pTM1 via the BamHI/SmaI sites (ATG: biosynthetics, Merzhausen, Germany). .. The plasmids were transformed into DH10Bactm E. coli cells to generate high molecular weight recombinant bacmid DNA which was then used to transfect Sf9 insect cells to obtain virus stocks .

Recombinant:

Article Title: Expression and characterization of recombinant Par j 1 and Par j 2 resembling the allergenic epitopes of Parietaria judaica pollen
Article Snippet: .. Cloning and generating of high molecular weight recombinant bacmid DNA Synthetic genes, codon-optimized for expression in insect cells coding for Par j 2.0101 (340 base pairs; accession number P55958) and for Par j 1.0102 (451 base pairs; accession number O04404) both including an additional 3′ sequence coding for a C-terminal hexahisitidine tag (Table ), were subcloned into plasmid pTM1 via the BamHI/SmaI sites (ATG: biosynthetics, Merzhausen, Germany). .. The plasmids were transformed into DH10Bactm E. coli cells to generate high molecular weight recombinant bacmid DNA which was then used to transfect Sf9 insect cells to obtain virus stocks .

Plasmid Preparation:

Article Title: Expression and characterization of recombinant Par j 1 and Par j 2 resembling the allergenic epitopes of Parietaria judaica pollen
Article Snippet: .. Cloning and generating of high molecular weight recombinant bacmid DNA Synthetic genes, codon-optimized for expression in insect cells coding for Par j 2.0101 (340 base pairs; accession number P55958) and for Par j 1.0102 (451 base pairs; accession number O04404) both including an additional 3′ sequence coding for a C-terminal hexahisitidine tag (Table ), were subcloned into plasmid pTM1 via the BamHI/SmaI sites (ATG: biosynthetics, Merzhausen, Germany). .. The plasmids were transformed into DH10Bactm E. coli cells to generate high molecular weight recombinant bacmid DNA which was then used to transfect Sf9 insect cells to obtain virus stocks .