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Roche bamhi sites
Bamhi Sites, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi sites/product/Roche
Average 93 stars, based on 11 article reviews
Price from $9.99 to $1999.99
bamhi sites - by Bioz Stars, 2020-07
93/100 stars

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Polymerase Chain Reaction:

Article Title: Head-to-tail regulation is critical for the in vivo function of myosin V
Article Snippet: .. The region −500 to +750 (containing the promoter and the first 750 nucleotides of MYO2 ) was amplified from genomic DNA of a wild-type BY4741 strain with XhoI and BamHI sites at the end of the forward and reverse primers, respectively, using the Expand High Fidelity PCR kit (Roche). .. After overnight digestion, this was then ligated into a similarly cut pRS303 vector.

Article Title: Host proteins interacting with the Moloney murine leukemia virus integrase: Multiple transcriptional regulators and chromatin binding factors
Article Snippet: .. The mIN insert was subcloned into the EcoRI and BamHI sites by PCR (Expand High Fidelity, Roche) using the primer pairs listed in Table S2 in Additional file , generating plasmid mIN-pNlexA. .. MoMLV Integrase was subcloned into the GAL4 DNA binding domain vector pGBKT7 (Clontech, USA) by insertion of the EcoRI-SalI integrase fragment from pSH2-mIN to generate pGBKT7-mIN.

Clone Assay:

Article Title: Rab14 Regulates Maturation of Macrophage Phagosomes Containing the Fungal Pathogen Candida albicans and Outcome of the Host-Pathogen Interaction
Article Snippet: .. Murine Rab5 cDNA was amplified from a plasmid (OriGene, Rockville, MD, USA) by using a proofreading polymerase (KOD; Merck Millipore, Darmstadt, Germany) with primers 5′-GCCGCCGAATTCCCATGGCTAATCGA-3′ and 5′-GAGCGGCCGGGATCCTAGTTACTACA-3′ for Rab5 (Eurofins Genomics, Ebersberg, Germany) to generate EcoRI and BamHI sites (Roche, Welwyn Garden City, United Kingdom) for cloning into pEGFP-C3 (Clontech, Mountain View, CA, USA). .. Murine Rab7 cDNA was digested from a plasmid (OriGene) with SgfI and MluI (Roche) and was inserted into pCMV-AN-tRFP (OriGene).

Amplification:

Article Title: Rab14 Regulates Maturation of Macrophage Phagosomes Containing the Fungal Pathogen Candida albicans and Outcome of the Host-Pathogen Interaction
Article Snippet: .. Murine Rab5 cDNA was amplified from a plasmid (OriGene, Rockville, MD, USA) by using a proofreading polymerase (KOD; Merck Millipore, Darmstadt, Germany) with primers 5′-GCCGCCGAATTCCCATGGCTAATCGA-3′ and 5′-GAGCGGCCGGGATCCTAGTTACTACA-3′ for Rab5 (Eurofins Genomics, Ebersberg, Germany) to generate EcoRI and BamHI sites (Roche, Welwyn Garden City, United Kingdom) for cloning into pEGFP-C3 (Clontech, Mountain View, CA, USA). .. Murine Rab7 cDNA was digested from a plasmid (OriGene) with SgfI and MluI (Roche) and was inserted into pCMV-AN-tRFP (OriGene).

Article Title: Head-to-tail regulation is critical for the in vivo function of myosin V
Article Snippet: .. The region −500 to +750 (containing the promoter and the first 750 nucleotides of MYO2 ) was amplified from genomic DNA of a wild-type BY4741 strain with XhoI and BamHI sites at the end of the forward and reverse primers, respectively, using the Expand High Fidelity PCR kit (Roche). .. After overnight digestion, this was then ligated into a similarly cut pRS303 vector.

Plasmid Preparation:

Article Title: Rab14 Regulates Maturation of Macrophage Phagosomes Containing the Fungal Pathogen Candida albicans and Outcome of the Host-Pathogen Interaction
Article Snippet: .. Murine Rab5 cDNA was amplified from a plasmid (OriGene, Rockville, MD, USA) by using a proofreading polymerase (KOD; Merck Millipore, Darmstadt, Germany) with primers 5′-GCCGCCGAATTCCCATGGCTAATCGA-3′ and 5′-GAGCGGCCGGGATCCTAGTTACTACA-3′ for Rab5 (Eurofins Genomics, Ebersberg, Germany) to generate EcoRI and BamHI sites (Roche, Welwyn Garden City, United Kingdom) for cloning into pEGFP-C3 (Clontech, Mountain View, CA, USA). .. Murine Rab7 cDNA was digested from a plasmid (OriGene) with SgfI and MluI (Roche) and was inserted into pCMV-AN-tRFP (OriGene).

Article Title: Host proteins interacting with the Moloney murine leukemia virus integrase: Multiple transcriptional regulators and chromatin binding factors
Article Snippet: .. The mIN insert was subcloned into the EcoRI and BamHI sites by PCR (Expand High Fidelity, Roche) using the primer pairs listed in Table S2 in Additional file , generating plasmid mIN-pNlexA. .. MoMLV Integrase was subcloned into the GAL4 DNA binding domain vector pGBKT7 (Clontech, USA) by insertion of the EcoRI-SalI integrase fragment from pSH2-mIN to generate pGBKT7-mIN.

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    Roche bamhi psti site
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    Roche kpni bamhi restriction sites
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