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GenScript bamhi sites
Bamhi Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi sites/product/GenScript
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
bamhi sites - by Bioz Stars, 2020-07
92/100 stars

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Clone Assay:

Article Title: Following the Evolutionary Track of a Highly Specific l-Arginine Oxidase by Reconstruction and Biochemical Analysis of Ancestral and Native Enzymes
Article Snippet: .. Genes encoding a native AROD candidate from Oceanobacter kriegii (OkAROD) (GI no. 654842541) and three ancestral ARODs (AncARODn0, AncARODn1, and AncARODn2) (see Table S2 in the supplemental material) were synthesized and cloned into the pET15b vector via the NdeI and BamHI sites by using GeneScript. ..

Article Title: MicroRNA-206 Targets notch3, Activates Apoptosis, and Inhibits Tumor Cell Migration and Focus Formation *
Article Snippet: .. Mouse genomic regions containing the Mus musculus miR-206 hairpins and ∼150 nucleotides of 5′- and 3′-flanking regions were cloned into the pRNAT-CMV3.2 vector between the XhoI and BamHI sites (GenScript, Piscataway, NJ). .. The specific miR-206 hairpin inhibitor (IH-310462-07) and miRIDIAN miRNA inhibitor negative control 1 (IN-001000-01) were purchased from Dharmacon (Lafayette CO).

Synthesized:

Article Title: In vivo polyester immobilized sortase for tagless protein purification
Article Snippet: .. Plasmid construction The PhaC-SrtA expressing plasmid (pET14:PhaC-SrtA) was constructed as follows: The srtA gene minus the N-terminal 59 amino acid membrane anchor region flanked by XhoI and BamHI sites was synthesized by Genscript, the product was cleaved with Xho I and Bam HI and ligated into the corresponding sites on the plasmid pET14b:phaC-linker-MalE [ ]. .. To make the tripartite fusion protein expressing plasmids (pET14:PhaC-SrtA-GFP, pET14:PhaC-SrtA-MBP, pET14:PhaC-SrtA-RV1626) the srtAΔN59 region was amplified from pET14:PhaC-SrtA with the primers SrtAN59_F and SrtAΔTAA_LPETG_R—this product does not have a stop codon and has LPETG coding region added on the C-terminus (with the TG encoded by an Age I site).

Article Title: Following the Evolutionary Track of a Highly Specific l-Arginine Oxidase by Reconstruction and Biochemical Analysis of Ancestral and Native Enzymes
Article Snippet: .. Genes encoding a native AROD candidate from Oceanobacter kriegii (OkAROD) (GI no. 654842541) and three ancestral ARODs (AncARODn0, AncARODn1, and AncARODn2) (see Table S2 in the supplemental material) were synthesized and cloned into the pET15b vector via the NdeI and BamHI sites by using GeneScript. ..

Article Title: Discovery and Characterization of the First Archaeal Dihydromethanopterin Reductase, an Iron-Sulfur Flavoprotein from Methanosarcina mazei
Article Snippet: .. The gene for MJ0208 (the MM1854 homolog from M. jannaschii ; gi number 2494448) was codon optimized for E. coli , synthesized with 5′ NdeI and 3′ BamHI sites, and subcloned into pET15b by GenScript (Piscataway, NJ). .. The plasmid was transformed into chemically competent BE100 cells.

Article Title: Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry
Article Snippet: .. Phage bIL67 RuvC mutant constructs An E. coli codon-optimized version ( ) of the Lactococcus lactis phage bIL67 ruvC (ORF23 ) gene (Schouler et al ., ) was synthesized and inserted into the T7 expression vector pET24a(+) at NdeI and BamHI sites by GenScript to create p67RuvCwt. .. Site-directed mutant derivatives of this clone were also generated by the company at the same time.

Article Title: Mn-TAT PTD-Ngb attenuates oxidative injury by an enhanced ROS scavenging ability and the regulation of redox signaling pathway
Article Snippet: .. Construction of the TAT PTD-Ngb plasmid and expression of the fusion protein An Ngb gene corresponding to the sequence of the cDNA clone, which contains the TAT PTD sequence, was chemically synthesized with 5′ NcoI and 3′ BamHI sites as well as codon optimization for expression in E. coli and was subcloned into the EcoRV site of pUC57-Kan (GenScript, China). .. The synthetic Ngb gene was cloned as an NcoI/BamHI digest from pUC57-Kan-TAT PTD-Ngb into the expression vector pET30a (+) to produce a fusion with six histidine residues at the 5′ end.

Mutagenesis:

Article Title: Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry
Article Snippet: .. Phage bIL67 RuvC mutant constructs An E. coli codon-optimized version ( ) of the Lactococcus lactis phage bIL67 ruvC (ORF23 ) gene (Schouler et al ., ) was synthesized and inserted into the T7 expression vector pET24a(+) at NdeI and BamHI sites by GenScript to create p67RuvCwt. .. Site-directed mutant derivatives of this clone were also generated by the company at the same time.

Construct:

Article Title: In vivo polyester immobilized sortase for tagless protein purification
Article Snippet: .. Plasmid construction The PhaC-SrtA expressing plasmid (pET14:PhaC-SrtA) was constructed as follows: The srtA gene minus the N-terminal 59 amino acid membrane anchor region flanked by XhoI and BamHI sites was synthesized by Genscript, the product was cleaved with Xho I and Bam HI and ligated into the corresponding sites on the plasmid pET14b:phaC-linker-MalE [ ]. .. To make the tripartite fusion protein expressing plasmids (pET14:PhaC-SrtA-GFP, pET14:PhaC-SrtA-MBP, pET14:PhaC-SrtA-RV1626) the srtAΔN59 region was amplified from pET14:PhaC-SrtA with the primers SrtAN59_F and SrtAΔTAA_LPETG_R—this product does not have a stop codon and has LPETG coding region added on the C-terminus (with the TG encoded by an Age I site).

Article Title: The neurodegenerative diseases ALS and SMA are linked at the molecular level via the ASC-1 complex
Article Snippet: .. The HA-TRIP4 plasmid was constructed by inserting the coding sequence of TRIP4 into the KpnI and BamHI sites of pcDNA3.1+N-HA using Clone EZ technology (GenScript, Piscataway, NJ, USA). .. The HA-TRIP41-254 plasmid was generated by mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and the HA-TRIP4 plasmid as template.

Article Title: Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry
Article Snippet: .. Phage bIL67 RuvC mutant constructs An E. coli codon-optimized version ( ) of the Lactococcus lactis phage bIL67 ruvC (ORF23 ) gene (Schouler et al ., ) was synthesized and inserted into the T7 expression vector pET24a(+) at NdeI and BamHI sites by GenScript to create p67RuvCwt. .. Site-directed mutant derivatives of this clone were also generated by the company at the same time.

Expressing:

Article Title: In vivo polyester immobilized sortase for tagless protein purification
Article Snippet: .. Plasmid construction The PhaC-SrtA expressing plasmid (pET14:PhaC-SrtA) was constructed as follows: The srtA gene minus the N-terminal 59 amino acid membrane anchor region flanked by XhoI and BamHI sites was synthesized by Genscript, the product was cleaved with Xho I and Bam HI and ligated into the corresponding sites on the plasmid pET14b:phaC-linker-MalE [ ]. .. To make the tripartite fusion protein expressing plasmids (pET14:PhaC-SrtA-GFP, pET14:PhaC-SrtA-MBP, pET14:PhaC-SrtA-RV1626) the srtAΔN59 region was amplified from pET14:PhaC-SrtA with the primers SrtAN59_F and SrtAΔTAA_LPETG_R—this product does not have a stop codon and has LPETG coding region added on the C-terminus (with the TG encoded by an Age I site).

Article Title: Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry
Article Snippet: .. Phage bIL67 RuvC mutant constructs An E. coli codon-optimized version ( ) of the Lactococcus lactis phage bIL67 ruvC (ORF23 ) gene (Schouler et al ., ) was synthesized and inserted into the T7 expression vector pET24a(+) at NdeI and BamHI sites by GenScript to create p67RuvCwt. .. Site-directed mutant derivatives of this clone were also generated by the company at the same time.

Article Title: Mn-TAT PTD-Ngb attenuates oxidative injury by an enhanced ROS scavenging ability and the regulation of redox signaling pathway
Article Snippet: .. Construction of the TAT PTD-Ngb plasmid and expression of the fusion protein An Ngb gene corresponding to the sequence of the cDNA clone, which contains the TAT PTD sequence, was chemically synthesized with 5′ NcoI and 3′ BamHI sites as well as codon optimization for expression in E. coli and was subcloned into the EcoRV site of pUC57-Kan (GenScript, China). .. The synthetic Ngb gene was cloned as an NcoI/BamHI digest from pUC57-Kan-TAT PTD-Ngb into the expression vector pET30a (+) to produce a fusion with six histidine residues at the 5′ end.

Sequencing:

Article Title: The neurodegenerative diseases ALS and SMA are linked at the molecular level via the ASC-1 complex
Article Snippet: .. The HA-TRIP4 plasmid was constructed by inserting the coding sequence of TRIP4 into the KpnI and BamHI sites of pcDNA3.1+N-HA using Clone EZ technology (GenScript, Piscataway, NJ, USA). .. The HA-TRIP41-254 plasmid was generated by mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and the HA-TRIP4 plasmid as template.

Article Title: Mn-TAT PTD-Ngb attenuates oxidative injury by an enhanced ROS scavenging ability and the regulation of redox signaling pathway
Article Snippet: .. Construction of the TAT PTD-Ngb plasmid and expression of the fusion protein An Ngb gene corresponding to the sequence of the cDNA clone, which contains the TAT PTD sequence, was chemically synthesized with 5′ NcoI and 3′ BamHI sites as well as codon optimization for expression in E. coli and was subcloned into the EcoRV site of pUC57-Kan (GenScript, China). .. The synthetic Ngb gene was cloned as an NcoI/BamHI digest from pUC57-Kan-TAT PTD-Ngb into the expression vector pET30a (+) to produce a fusion with six histidine residues at the 5′ end.

Plasmid Preparation:

Article Title: In vivo polyester immobilized sortase for tagless protein purification
Article Snippet: .. Plasmid construction The PhaC-SrtA expressing plasmid (pET14:PhaC-SrtA) was constructed as follows: The srtA gene minus the N-terminal 59 amino acid membrane anchor region flanked by XhoI and BamHI sites was synthesized by Genscript, the product was cleaved with Xho I and Bam HI and ligated into the corresponding sites on the plasmid pET14b:phaC-linker-MalE [ ]. .. To make the tripartite fusion protein expressing plasmids (pET14:PhaC-SrtA-GFP, pET14:PhaC-SrtA-MBP, pET14:PhaC-SrtA-RV1626) the srtAΔN59 region was amplified from pET14:PhaC-SrtA with the primers SrtAN59_F and SrtAΔTAA_LPETG_R—this product does not have a stop codon and has LPETG coding region added on the C-terminus (with the TG encoded by an Age I site).

Article Title: Following the Evolutionary Track of a Highly Specific l-Arginine Oxidase by Reconstruction and Biochemical Analysis of Ancestral and Native Enzymes
Article Snippet: .. Genes encoding a native AROD candidate from Oceanobacter kriegii (OkAROD) (GI no. 654842541) and three ancestral ARODs (AncARODn0, AncARODn1, and AncARODn2) (see Table S2 in the supplemental material) were synthesized and cloned into the pET15b vector via the NdeI and BamHI sites by using GeneScript. ..

Article Title: Development and Applications of a Calmodulin-Based Fusion Protein System for the Expression and Purification of WW and Zinc Finger Modules
Article Snippet: .. The gene, subcloned into the NdeI and BamHI sites of a pET21-a(+) vector, was ordered from Genscript. .. These restriction enzyme sites were selected as they would result in the gene of interest occurring some distance from the His tag found in the pET-21 systems and with the T7 tag (which occurs between NdeI and BamHI in this pET construct) removed.

Article Title: The neurodegenerative diseases ALS and SMA are linked at the molecular level via the ASC-1 complex
Article Snippet: .. The HA-TRIP4 plasmid was constructed by inserting the coding sequence of TRIP4 into the KpnI and BamHI sites of pcDNA3.1+N-HA using Clone EZ technology (GenScript, Piscataway, NJ, USA). .. The HA-TRIP41-254 plasmid was generated by mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and the HA-TRIP4 plasmid as template.

Article Title: Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry
Article Snippet: .. Phage bIL67 RuvC mutant constructs An E. coli codon-optimized version ( ) of the Lactococcus lactis phage bIL67 ruvC (ORF23 ) gene (Schouler et al ., ) was synthesized and inserted into the T7 expression vector pET24a(+) at NdeI and BamHI sites by GenScript to create p67RuvCwt. .. Site-directed mutant derivatives of this clone were also generated by the company at the same time.

Article Title: Mn-TAT PTD-Ngb attenuates oxidative injury by an enhanced ROS scavenging ability and the regulation of redox signaling pathway
Article Snippet: .. Construction of the TAT PTD-Ngb plasmid and expression of the fusion protein An Ngb gene corresponding to the sequence of the cDNA clone, which contains the TAT PTD sequence, was chemically synthesized with 5′ NcoI and 3′ BamHI sites as well as codon optimization for expression in E. coli and was subcloned into the EcoRV site of pUC57-Kan (GenScript, China). .. The synthetic Ngb gene was cloned as an NcoI/BamHI digest from pUC57-Kan-TAT PTD-Ngb into the expression vector pET30a (+) to produce a fusion with six histidine residues at the 5′ end.

Article Title: MicroRNA-206 Targets notch3, Activates Apoptosis, and Inhibits Tumor Cell Migration and Focus Formation *
Article Snippet: .. Mouse genomic regions containing the Mus musculus miR-206 hairpins and ∼150 nucleotides of 5′- and 3′-flanking regions were cloned into the pRNAT-CMV3.2 vector between the XhoI and BamHI sites (GenScript, Piscataway, NJ). .. The specific miR-206 hairpin inhibitor (IH-310462-07) and miRIDIAN miRNA inhibitor negative control 1 (IN-001000-01) were purchased from Dharmacon (Lafayette CO).

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    GenScript bamhi restriction enzyme site
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    https://www.bioz.com/result/bamhi restriction enzyme site/product/GenScript
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    GenScript bamhi xhoi sites
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    GenScript c terminal bamhi restriction sites
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    GenScript bamh1 restriction sites
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