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GenScript bamhi site
Bamhi Site, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi site/product/GenScript
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
bamhi site - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Clone Assay:

Article Title: Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo
Article Snippet: .. Defensin-1 cloning, expression and purification The cDNA fragment optimised for codon usage in Spodoptera frugiperda (Sf9) cells coding the signal peptide and mature bee defensin-1 followed by a (His)6 -tag with XhoI site on the N-terminal and BamHI site on the C-terminal was synthesised and cloned into a BlueSript plasmid (GenScript, Hong Kong). .. The purchased BlueScript-Def plasmid was digested with XhoI and BamHI .

Article Title: Identification and characterization of functional homologs of nitrogenase cofactor biosynthesis protein NifB from methanogens
Article Snippet: .. Genes encoding the NifB homologs from M. acetivorans (NifBMa ) and M. thermoautotrophicum (NifBMt ) were codon optimized for E. coli expression and synthesized and cloned into the BamHI site of pET-3b and the NdeI site of pET-14b, respectively (GenScript USA). .. A short sequence encoding a 6xHis tag was inserted at the 5′-end of the gene encoding NifBMa before the cloning of this sequence into pET-3b, whereas the gene encoding NifBMt was placed behind a vector-derived sequence encoding a 6xHis tag when it was cloned into pET-14b.

Article Title: Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence
Article Snippet: .. A DNA fragment containing the LAI sequences between the SalI site and the env start codon, followed by the ConM env sequence up to the BamHI site, was synthesized and cloned into the LAI molecular clone backbone using SalI and BamHI (Genscript, Piscataway, NJ). .. The resulting molecular clone encodes the complete ConM gp160 sequence, except for the C-terminal 106 amino acids of the cytoplasmic tail, which are derived from LAI gp160.

Positron Emission Tomography:

Article Title: Identification and characterization of functional homologs of nitrogenase cofactor biosynthesis protein NifB from methanogens
Article Snippet: .. Genes encoding the NifB homologs from M. acetivorans (NifBMa ) and M. thermoautotrophicum (NifBMt ) were codon optimized for E. coli expression and synthesized and cloned into the BamHI site of pET-3b and the NdeI site of pET-14b, respectively (GenScript USA). .. A short sequence encoding a 6xHis tag was inserted at the 5′-end of the gene encoding NifBMa before the cloning of this sequence into pET-3b, whereas the gene encoding NifBMt was placed behind a vector-derived sequence encoding a 6xHis tag when it was cloned into pET-14b.

Synthesized:

Article Title: Identification and characterization of functional homologs of nitrogenase cofactor biosynthesis protein NifB from methanogens
Article Snippet: .. Genes encoding the NifB homologs from M. acetivorans (NifBMa ) and M. thermoautotrophicum (NifBMt ) were codon optimized for E. coli expression and synthesized and cloned into the BamHI site of pET-3b and the NdeI site of pET-14b, respectively (GenScript USA). .. A short sequence encoding a 6xHis tag was inserted at the 5′-end of the gene encoding NifBMa before the cloning of this sequence into pET-3b, whereas the gene encoding NifBMt was placed behind a vector-derived sequence encoding a 6xHis tag when it was cloned into pET-14b.

Article Title: Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence
Article Snippet: .. A DNA fragment containing the LAI sequences between the SalI site and the env start codon, followed by the ConM env sequence up to the BamHI site, was synthesized and cloned into the LAI molecular clone backbone using SalI and BamHI (Genscript, Piscataway, NJ). .. The resulting molecular clone encodes the complete ConM gp160 sequence, except for the C-terminal 106 amino acids of the cytoplasmic tail, which are derived from LAI gp160.

Mutagenesis:

Article Title: Characterization of Leber Congenital Amaurosis-associated NMNAT1 Mutants *
Article Snippet: .. For bacterial expression of wild type and mutant NMNAT1 proteins, the respective cDNAs were subcloned into the BamHI site of pET30a. .. Mouse dorsal root ganglion (DRG) were dissected from E13.5 CD1 embryo and cultured in 96-well plates coated with poly- d -lysine (0.1 mg/ml; Sigma) and laminin (3 μg/ml; Invitrogen) as previously described ( ).

Construct:

Article Title: Dynamic binding of replication protein a is required for DNA repair
Article Snippet: .. Protein purification The construct for expression of the biotinylated RPA3 was constructed by synthesizing a synthetic coding sequence that contained an XbaI site, an N-terminal BirA recognition sequence (BAP:GLNDIFEAQKIEWHW) , a six histidine His-Tag and the coding sequence for RPA3 with codon usage optimized for expression in E. coli followed by a BamHI site (GenScript). .. This sequence was then used to replace the existing RPA3 gene in p11d-tRPA containing wild-type RPA ( ) using XbaI and BamHI.

Article Title: Characterization of Leber Congenital Amaurosis-associated NMNAT1 Mutants *
Article Snippet: .. NMNAT1 mutants were constructed with either N-terminal HA or FLAG epitope tag by gene synthesis and inserted into the BamHI site of pUC57 (GeneScript). .. The clones constructed included: FLAG-wild type, FLAG-V9M, FLAG-A13T, FLAG-D33G, FLAG-R66W, FLAG-I217N, FLAG-E257K, FLAG-STP280Gln, HA-wt, HA-M69V, HA-V98G, HA-V151F, HA-D173G, HA-R207W, HA-R237C, and HA-L239S.

Purification:

Article Title: Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo
Article Snippet: .. Defensin-1 cloning, expression and purification The cDNA fragment optimised for codon usage in Spodoptera frugiperda (Sf9) cells coding the signal peptide and mature bee defensin-1 followed by a (His)6 -tag with XhoI site on the N-terminal and BamHI site on the C-terminal was synthesised and cloned into a BlueSript plasmid (GenScript, Hong Kong). .. The purchased BlueScript-Def plasmid was digested with XhoI and BamHI .

Protein Purification:

Article Title: Dynamic binding of replication protein a is required for DNA repair
Article Snippet: .. Protein purification The construct for expression of the biotinylated RPA3 was constructed by synthesizing a synthetic coding sequence that contained an XbaI site, an N-terminal BirA recognition sequence (BAP:GLNDIFEAQKIEWHW) , a six histidine His-Tag and the coding sequence for RPA3 with codon usage optimized for expression in E. coli followed by a BamHI site (GenScript). .. This sequence was then used to replace the existing RPA3 gene in p11d-tRPA containing wild-type RPA ( ) using XbaI and BamHI.

FLAG-tag:

Article Title: Characterization of Leber Congenital Amaurosis-associated NMNAT1 Mutants *
Article Snippet: .. NMNAT1 mutants were constructed with either N-terminal HA or FLAG epitope tag by gene synthesis and inserted into the BamHI site of pUC57 (GeneScript). .. The clones constructed included: FLAG-wild type, FLAG-V9M, FLAG-A13T, FLAG-D33G, FLAG-R66W, FLAG-I217N, FLAG-E257K, FLAG-STP280Gln, HA-wt, HA-M69V, HA-V98G, HA-V151F, HA-D173G, HA-R207W, HA-R237C, and HA-L239S.

Expressing:

Article Title: Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo
Article Snippet: .. Defensin-1 cloning, expression and purification The cDNA fragment optimised for codon usage in Spodoptera frugiperda (Sf9) cells coding the signal peptide and mature bee defensin-1 followed by a (His)6 -tag with XhoI site on the N-terminal and BamHI site on the C-terminal was synthesised and cloned into a BlueSript plasmid (GenScript, Hong Kong). .. The purchased BlueScript-Def plasmid was digested with XhoI and BamHI .

Article Title: Characterization of Leber Congenital Amaurosis-associated NMNAT1 Mutants *
Article Snippet: .. For bacterial expression of wild type and mutant NMNAT1 proteins, the respective cDNAs were subcloned into the BamHI site of pET30a. .. Mouse dorsal root ganglion (DRG) were dissected from E13.5 CD1 embryo and cultured in 96-well plates coated with poly- d -lysine (0.1 mg/ml; Sigma) and laminin (3 μg/ml; Invitrogen) as previously described ( ).

Article Title: Identification and characterization of functional homologs of nitrogenase cofactor biosynthesis protein NifB from methanogens
Article Snippet: .. Genes encoding the NifB homologs from M. acetivorans (NifBMa ) and M. thermoautotrophicum (NifBMt ) were codon optimized for E. coli expression and synthesized and cloned into the BamHI site of pET-3b and the NdeI site of pET-14b, respectively (GenScript USA). .. A short sequence encoding a 6xHis tag was inserted at the 5′-end of the gene encoding NifBMa before the cloning of this sequence into pET-3b, whereas the gene encoding NifBMt was placed behind a vector-derived sequence encoding a 6xHis tag when it was cloned into pET-14b.

Article Title: Dynamic binding of replication protein a is required for DNA repair
Article Snippet: .. Protein purification The construct for expression of the biotinylated RPA3 was constructed by synthesizing a synthetic coding sequence that contained an XbaI site, an N-terminal BirA recognition sequence (BAP:GLNDIFEAQKIEWHW) , a six histidine His-Tag and the coding sequence for RPA3 with codon usage optimized for expression in E. coli followed by a BamHI site (GenScript). .. This sequence was then used to replace the existing RPA3 gene in p11d-tRPA containing wild-type RPA ( ) using XbaI and BamHI.

Article Title: Characterization of Leber Congenital Amaurosis-associated NMNAT1 Mutants *
Article Snippet: .. The fragments were transferred into the BamHI site of the lentivirus vector FCIV that includes a downstream IRES Venus for monitoring expression ( ). .. The E257Q and E257D NMNAT1 mutants were generated using mega primer PCR and cloned into the BamHI site of FCIV by InFusion (Clontech).

Sequencing:

Article Title: Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence
Article Snippet: .. A DNA fragment containing the LAI sequences between the SalI site and the env start codon, followed by the ConM env sequence up to the BamHI site, was synthesized and cloned into the LAI molecular clone backbone using SalI and BamHI (Genscript, Piscataway, NJ). .. The resulting molecular clone encodes the complete ConM gp160 sequence, except for the C-terminal 106 amino acids of the cytoplasmic tail, which are derived from LAI gp160.

Article Title: Dynamic binding of replication protein a is required for DNA repair
Article Snippet: .. Protein purification The construct for expression of the biotinylated RPA3 was constructed by synthesizing a synthetic coding sequence that contained an XbaI site, an N-terminal BirA recognition sequence (BAP:GLNDIFEAQKIEWHW) , a six histidine His-Tag and the coding sequence for RPA3 with codon usage optimized for expression in E. coli followed by a BamHI site (GenScript). .. This sequence was then used to replace the existing RPA3 gene in p11d-tRPA containing wild-type RPA ( ) using XbaI and BamHI.

Plasmid Preparation:

Article Title: Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo
Article Snippet: .. Defensin-1 cloning, expression and purification The cDNA fragment optimised for codon usage in Spodoptera frugiperda (Sf9) cells coding the signal peptide and mature bee defensin-1 followed by a (His)6 -tag with XhoI site on the N-terminal and BamHI site on the C-terminal was synthesised and cloned into a BlueSript plasmid (GenScript, Hong Kong). .. The purchased BlueScript-Def plasmid was digested with XhoI and BamHI .

Article Title: Characterization of Leber Congenital Amaurosis-associated NMNAT1 Mutants *
Article Snippet: .. The fragments were transferred into the BamHI site of the lentivirus vector FCIV that includes a downstream IRES Venus for monitoring expression ( ). .. The E257Q and E257D NMNAT1 mutants were generated using mega primer PCR and cloned into the BamHI site of FCIV by InFusion (Clontech).

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    GenScript bamhi restriction enzyme site
    Bamhi Restriction Enzyme Site, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi restriction enzyme site/product/GenScript
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    92
    GenScript bamhi xhoi sites
    Bamhi Xhoi Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi xhoi sites/product/GenScript
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bamhi xhoi sites - by Bioz Stars, 2020-07
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    88
    GenScript c terminal bamhi restriction sites
    C Terminal Bamhi Restriction Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c terminal bamhi restriction sites/product/GenScript
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c terminal bamhi restriction sites - by Bioz Stars, 2020-07
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    GenScript bamh1 restriction sites
    Bamh1 Restriction Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamh1 restriction sites/product/GenScript
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bamh1 restriction sites - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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