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GE Healthcare bamhi site
Bamhi Site, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi site/product/GE Healthcare
Average 92 stars, based on 39 article reviews
Price from $9.99 to $1999.99
bamhi site - by Bioz Stars, 2020-07
92/100 stars

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Clone Assay:

Article Title: Interference with Immunoglobulin (Ig)? Immunoreceptor Tyrosine-Based Activation Motif (Itam) Phosphorylation Modulates or Blocks B Cell Development, Depending on the Availability of an Ig? Cytoplasmic Tail
Article Snippet: .. The 5.2-kb BamHI fragment was then cloned into the BamHI site of pSL1190XX to obtain pSL5.2kbloxP. pSL1190XX was generated from pSL1190 (Amersham Pharmacia Biotech) by deleting the polylinker-region between XhoI and XbaI. ..

Polymerase Chain Reaction:

Article Title: Antibodies against Merozoite Surface Protein (Msp)-119 Are a Major Component of the Invasion-Inhibitory Response in Individuals Immune to Malaria
Article Snippet: .. The resulting PCR products were ligated into the BamHI site of pGEX-4T-1, expressed as glutathione S transferase (GST) fusion proteins in Escherichia coli and purified using glutathine-sepharose as described by the manufacturer (Amersham Pharmacia Biotech). .. GST alone was produced using the pGEX-4T-1 plasmid.

Mutagenesis:

Article Title: Treatment of Experimental (Trinitrobenzene Sulfonic Acid) Colitis by Intranasal Administration of Transforming Growth Factor (Tgf)-?1 Plasmid
Article Snippet: .. The mutant TGF-β1 cDNA thus obtained was subcloned into the BamHI site of the pSL1180 superlinker vector (Amersham Pharmacia Biotech), from which it was excised with EcoRI and EcoRV and ligated into the EcoRI and SmaI sites of the pCI vector under the control of CMV promotor (Promega). .. The vector obtained was designated pCMV-TGF-β.

Construct:

Article Title: Anti-Candidal Activity of Genetically Engineered Histatin Variants with Multiple Functional Domains
Article Snippet: .. Plasmid Construction and Transformation ReHst3 1-mer, reHst3 2-mer, reHst3 3-mer, and reHst3 4-mer constructs were cleaved with BamHI and ligated into the BamHI site of the pGEX-3X, which is a bacterial GST expression vector (GE Healthcare, Piscataway, NJ). .. Transformation of the resulting plasmids into JM109 cells was performed according to the manufacturer’s instructions.

Article Title: Probing the Conformation of the Fibronectin III1–2 Domain by Fluorescence Resonance Energy Transfer
Article Snippet: .. The CFP BamHI-BglII fragment of pGEM-CFP was inserted into the BamHI site of pGEX-6P-2 (GE Healthcare) upstream of human III1–2 to generate the donor-only construct CFP-III1–2 in pGEX or CIII. .. The gene expressing III1–2 -YFP with BamHI and EcoRI restriction sites at the 5′- and 3′-ends, respectively, was created by overlap extension PCR.

Article Title: Probing the Conformation of the Fibronectin III1–2 Domain by Fluorescence Resonance Energy Transfer
Article Snippet: .. The donor-acceptor construct, CFP-III1–2 -YFP or CIIIY, was created by ligating the BamHI-CFP-BglII fragment from pGEM-CFP to the BamHI site of the acceptor-only construct IIIY. .. All of the constructs were expressed as glutathione S -transferase (GST) fusion proteins in Escherichia coli DH12α.

Purification:

Article Title: Antibodies against Merozoite Surface Protein (Msp)-119 Are a Major Component of the Invasion-Inhibitory Response in Individuals Immune to Malaria
Article Snippet: .. The resulting PCR products were ligated into the BamHI site of pGEX-4T-1, expressed as glutathione S transferase (GST) fusion proteins in Escherichia coli and purified using glutathine-sepharose as described by the manufacturer (Amersham Pharmacia Biotech). .. GST alone was produced using the pGEX-4T-1 plasmid.

Modification:

Article Title: A Conserved Interaction between a C-Terminal Motif in Norovirus VPg and the HEAT-1 Domain of eIF4G Is Essential for Translation Initiation
Article Snippet: .. Two modified versions of pETM-11, one with a BamHI site replacing the NcoI site (pETM-11m1), and the other, a variant of pETM-11m1, in which a thrombin cleavage site downstream of the N-terminal His-tag replaces the Tobacco Etch Virus (TEV) NIa cleavage site (pETM-11m2) [ , ]. pMALX(E) has been described elsewhere [ ] and pGEX-2T is commercially available (GE healthcare). .. An additional modified pGEX-2T plasmid was used with a TEV NIa cleavage site replacing the thrombin site (pGEX-2Tm). eIF4GI, eIF4GII and DAP5 sequences were amplified by PCR (KOD polymerase hot start kit) from full length cDNA clones. cDNA for eIF4GI, eIF4GII and DAP5 were kindly supplied by Chris Hellen and Tatyana Pestova (State University New York), Mark Coldwell (University of Southampton) and Bhushan Nagar (McGill University, Montréal) respectively.

Generated:

Article Title: Interference with Immunoglobulin (Ig)? Immunoreceptor Tyrosine-Based Activation Motif (Itam) Phosphorylation Modulates or Blocks B Cell Development, Depending on the Availability of an Ig? Cytoplasmic Tail
Article Snippet: .. The 5.2-kb BamHI fragment was then cloned into the BamHI site of pSL1190XX to obtain pSL5.2kbloxP. pSL1190XX was generated from pSL1190 (Amersham Pharmacia Biotech) by deleting the polylinker-region between XhoI and XbaI. ..

Expressing:

Article Title: Anti-Candidal Activity of Genetically Engineered Histatin Variants with Multiple Functional Domains
Article Snippet: .. Plasmid Construction and Transformation ReHst3 1-mer, reHst3 2-mer, reHst3 3-mer, and reHst3 4-mer constructs were cleaved with BamHI and ligated into the BamHI site of the pGEX-3X, which is a bacterial GST expression vector (GE Healthcare, Piscataway, NJ). .. Transformation of the resulting plasmids into JM109 cells was performed according to the manufacturer’s instructions.

Article Title: eEF1A Is an S-RNase Binding Factor in Self-Incompatible Solanum chacoense
Article Snippet: .. The sequence was subcloned into the BamHI site of the pGEX-4T-2 protein expression vector (GE Healthcare Biosciences, PA) in frame with the N-terminal GST tag. .. The derived amino acid sequence of this pollen tube eEF1A contains all the amino acids determined by MS sequencing of the ConA column eluate.

Sequencing:

Article Title: eEF1A Is an S-RNase Binding Factor in Self-Incompatible Solanum chacoense
Article Snippet: .. The sequence was subcloned into the BamHI site of the pGEX-4T-2 protein expression vector (GE Healthcare Biosciences, PA) in frame with the N-terminal GST tag. .. The derived amino acid sequence of this pollen tube eEF1A contains all the amino acids determined by MS sequencing of the ConA column eluate.

Transformation Assay:

Article Title: Anti-Candidal Activity of Genetically Engineered Histatin Variants with Multiple Functional Domains
Article Snippet: .. Plasmid Construction and Transformation ReHst3 1-mer, reHst3 2-mer, reHst3 3-mer, and reHst3 4-mer constructs were cleaved with BamHI and ligated into the BamHI site of the pGEX-3X, which is a bacterial GST expression vector (GE Healthcare, Piscataway, NJ). .. Transformation of the resulting plasmids into JM109 cells was performed according to the manufacturer’s instructions.

Variant Assay:

Article Title: A Conserved Interaction between a C-Terminal Motif in Norovirus VPg and the HEAT-1 Domain of eIF4G Is Essential for Translation Initiation
Article Snippet: .. Two modified versions of pETM-11, one with a BamHI site replacing the NcoI site (pETM-11m1), and the other, a variant of pETM-11m1, in which a thrombin cleavage site downstream of the N-terminal His-tag replaces the Tobacco Etch Virus (TEV) NIa cleavage site (pETM-11m2) [ , ]. pMALX(E) has been described elsewhere [ ] and pGEX-2T is commercially available (GE healthcare). .. An additional modified pGEX-2T plasmid was used with a TEV NIa cleavage site replacing the thrombin site (pGEX-2Tm). eIF4GI, eIF4GII and DAP5 sequences were amplified by PCR (KOD polymerase hot start kit) from full length cDNA clones. cDNA for eIF4GI, eIF4GII and DAP5 were kindly supplied by Chris Hellen and Tatyana Pestova (State University New York), Mark Coldwell (University of Southampton) and Bhushan Nagar (McGill University, Montréal) respectively.

Plasmid Preparation:

Article Title: Anti-Candidal Activity of Genetically Engineered Histatin Variants with Multiple Functional Domains
Article Snippet: .. Plasmid Construction and Transformation ReHst3 1-mer, reHst3 2-mer, reHst3 3-mer, and reHst3 4-mer constructs were cleaved with BamHI and ligated into the BamHI site of the pGEX-3X, which is a bacterial GST expression vector (GE Healthcare, Piscataway, NJ). .. Transformation of the resulting plasmids into JM109 cells was performed according to the manufacturer’s instructions.

Article Title: eEF1A Is an S-RNase Binding Factor in Self-Incompatible Solanum chacoense
Article Snippet: .. The sequence was subcloned into the BamHI site of the pGEX-4T-2 protein expression vector (GE Healthcare Biosciences, PA) in frame with the N-terminal GST tag. .. The derived amino acid sequence of this pollen tube eEF1A contains all the amino acids determined by MS sequencing of the ConA column eluate.

Article Title: Treatment of Experimental (Trinitrobenzene Sulfonic Acid) Colitis by Intranasal Administration of Transforming Growth Factor (Tgf)-?1 Plasmid
Article Snippet: .. The mutant TGF-β1 cDNA thus obtained was subcloned into the BamHI site of the pSL1180 superlinker vector (Amersham Pharmacia Biotech), from which it was excised with EcoRI and EcoRV and ligated into the EcoRI and SmaI sites of the pCI vector under the control of CMV promotor (Promega). .. The vector obtained was designated pCMV-TGF-β.

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