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System Biosciences Inc bamhi restriction sites
Bamhi Restriction Sites, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi restriction sites/product/System Biosciences Inc
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
bamhi restriction sites - by Bioz Stars, 2020-07
92/100 stars

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Clone Assay:

Article Title: ARL11 regulates lipopolysaccharide-stimulated macrophage activation by promoting mitogen-activated protein kinase (MAPK) signaling
Article Snippet: .. For performing the rescue experiments, the human ARL11 gene with a C-terminal HA tag was cloned into the pCDH CMV MCS EF1-Hygro vector using NheI and BamHI restriction sites (System Biosciences). .. The mouse Arl11 gene with a C-terminal HA or Myc tag was cloned into pcDNA3.1(−) using cDNA prepared from RAW264.7 macrophages.

Article Title: Establishment of five immortalized human ovarian surface epithelial cell lines via SV40 T antigen or HPV E6/E7 expression
Article Snippet: .. The amplified cDNA was cloned into EcoRI and BamHI restriction sites of the pCDH-EF1-MCS-T2A-copGFP Lentivector (System Biosciences, Mountain View, CA). pLenti CMV/TO SV40 small+Large T vector was obtained from Addgene (Cambridge, MA). .. HEK293T cells (1 × 106 ) were co-transfected with 2 μg lentiviral vector and 2 μg pPACKH1 Lentivector Packaging Kit (System Biosciences, Palo Alto, CA).

Article Title: ARL11 regulates lipopolysaccharide-stimulated macrophage activation by promoting mitogen-activated protein kinase (MAPK) signaling
Article Snippet: .. For performing the rescue experiments, the human ARL11 gene with a C-terminal HA tag was cloned into the pCDH CMV MCS EF1-Hygro vector using NheI and BamHI restriction sites (System Biosciences). .. The mouse Arl11 gene with a C-terminal HA or Myc tag was cloned into pcDNA3.1(−) using cDNA prepared from RAW264.7 macrophages.

Amplification:

Article Title: Establishment of five immortalized human ovarian surface epithelial cell lines via SV40 T antigen or HPV E6/E7 expression
Article Snippet: .. The amplified cDNA was cloned into EcoRI and BamHI restriction sites of the pCDH-EF1-MCS-T2A-copGFP Lentivector (System Biosciences, Mountain View, CA). pLenti CMV/TO SV40 small+Large T vector was obtained from Addgene (Cambridge, MA). .. HEK293T cells (1 × 106 ) were co-transfected with 2 μg lentiviral vector and 2 μg pPACKH1 Lentivector Packaging Kit (System Biosciences, Palo Alto, CA).

Plasmid Preparation:

Article Title: ARL11 regulates lipopolysaccharide-stimulated macrophage activation by promoting mitogen-activated protein kinase (MAPK) signaling
Article Snippet: .. For performing the rescue experiments, the human ARL11 gene with a C-terminal HA tag was cloned into the pCDH CMV MCS EF1-Hygro vector using NheI and BamHI restriction sites (System Biosciences). .. The mouse Arl11 gene with a C-terminal HA or Myc tag was cloned into pcDNA3.1(−) using cDNA prepared from RAW264.7 macrophages.

Article Title: Establishment of five immortalized human ovarian surface epithelial cell lines via SV40 T antigen or HPV E6/E7 expression
Article Snippet: .. The amplified cDNA was cloned into EcoRI and BamHI restriction sites of the pCDH-EF1-MCS-T2A-copGFP Lentivector (System Biosciences, Mountain View, CA). pLenti CMV/TO SV40 small+Large T vector was obtained from Addgene (Cambridge, MA). .. HEK293T cells (1 × 106 ) were co-transfected with 2 μg lentiviral vector and 2 μg pPACKH1 Lentivector Packaging Kit (System Biosciences, Palo Alto, CA).

Article Title: ARL11 regulates lipopolysaccharide-stimulated macrophage activation by promoting mitogen-activated protein kinase (MAPK) signaling
Article Snippet: .. For performing the rescue experiments, the human ARL11 gene with a C-terminal HA tag was cloned into the pCDH CMV MCS EF1-Hygro vector using NheI and BamHI restriction sites (System Biosciences). .. The mouse Arl11 gene with a C-terminal HA or Myc tag was cloned into pcDNA3.1(−) using cDNA prepared from RAW264.7 macrophages.

Construct:

Article Title: Cellular Differentiation Regulator BLIMP1 Induces Epstein-Barr Virus Lytic Reactivation in Epithelial and B Cells by Activating Transcription from both the R and Z Promoters
Article Snippet: .. A BLIMP1-expressing lentivirus, CD713-BLIMP1, was constructed by insertion of the BLIMP1-coding sequences between the EcoRI and BamHI restriction sites of pCDH-MSCV-MCS-EF1-GFP-puro (CD713B-1; System Biosciences); the BLIMP1-coding DNA was obtained by digestion of pCDNA3-BLIMP1 DNA with EcoRI and BamHI restriction enzymes (NEB) and gel purification. .. Two lentiviruses expressing short hairpin RNAs (shRNAs) against BLIMP1 mRNA that had been constructed in pLKO.1-TRC were originally purchased from Thermo Scientific; they can now be obtained from Sigma-Aldrich (shRNA product SHCLNG_NM_001198; The RNAi Consortium [TRC] shRNAs TRCN0000013612 [BLIMP1 no. 1] and TRCN0000013610 [BLIMP1 no. 2]).

Gel Purification:

Article Title: Cellular Differentiation Regulator BLIMP1 Induces Epstein-Barr Virus Lytic Reactivation in Epithelial and B Cells by Activating Transcription from both the R and Z Promoters
Article Snippet: .. A BLIMP1-expressing lentivirus, CD713-BLIMP1, was constructed by insertion of the BLIMP1-coding sequences between the EcoRI and BamHI restriction sites of pCDH-MSCV-MCS-EF1-GFP-puro (CD713B-1; System Biosciences); the BLIMP1-coding DNA was obtained by digestion of pCDNA3-BLIMP1 DNA with EcoRI and BamHI restriction enzymes (NEB) and gel purification. .. Two lentiviruses expressing short hairpin RNAs (shRNAs) against BLIMP1 mRNA that had been constructed in pLKO.1-TRC were originally purchased from Thermo Scientific; they can now be obtained from Sigma-Aldrich (shRNA product SHCLNG_NM_001198; The RNAi Consortium [TRC] shRNAs TRCN0000013612 [BLIMP1 no. 1] and TRCN0000013610 [BLIMP1 no. 2]).

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    System Biosciences Inc bamh1 restriction sites
    Bamh1 Restriction Sites, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamh1 restriction sites/product/System Biosciences Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bamh1 restriction sites - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    92
    System Biosciences Inc bamhi restriction sites
    Bamhi Restriction Sites, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi restriction sites/product/System Biosciences Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bamhi restriction sites - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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