Structured Review

Promega bamhi restriction sites
Bamhi Restriction Sites, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi restriction sites/product/Promega
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
bamhi restriction sites - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Clone Assay:

Article Title: Type IIA Procollagen Containing the Cysteine-rich Amino Propeptide Is Deposited in the Extracellular Matrix of Prechondrogenic Tissue and Binds to TGF-?1 and BMP-2
Article Snippet: .. Primers (5′ primer, 5′-CGTGAATTCCAGGAGGCTGGCAGCTGTGTG-3′; 3′ primer, 5′-GATGGATCCGGCGAGGTCAGTTGGGCAGAT-3′) that flank the exon 2 splice site were used to amplify a 207-bp fragment with EcoRI and BamHI restriction sites from 54-d human fetal embryonic tissue total RNA by using reverse transcription-polymerase chain reaction (RT-PCR), and cloned into pGEM-3zf(+) expression vector ( Promega Corp. ). .. This construct was used to generate antisense and sense riboprobes by in vitro transcription for in situ hybridization.

Article Title: Signaling Lymphocytic Activation Molecule Family Receptor Homologs in New World Monkey Cytomegaloviruses
Article Snippet: .. An sbSLAMF6-Fc fusion protein containing the CD33 leader peptide and the Fc region of human IgG1 was obtained by chemically synthesizing the DNA sequences of the two Ig domains of S. boliviensis SLAMF6 with a splice donor site sequence flanked by BamHI restriction sites and cloning them into the pCI-neo Fc vector. pCI-neo Fc is derived from the pCI-neo vector (Promega) after introduction of the CD33 leader peptide and the Fc region of human IgG1 from the mammalian expression vector signal pIg-Tail (R & D Systems) within the XbaI and NotI restriction sites and disruption of its original BamHI site. .. In sbSLAMF6-Fc, a silent point mutation was included in the chemically synthesized sbSLAMF6 DNA sequence to eliminate the internal BamHI restriction site.

Luciferase:

Article Title: Conserved regions of the DMD 3’ UTR regulate translation and mRNA abundance in cultured myotubes
Article Snippet: .. The resulting 2,811 bp PCR product was digested with XbaI and AvrII and ligated into the XbaI and BamHI restriction sites of pHRL-CMV (Promega) immediately downstream of the Renilla Luciferase ORF. .. The resulting plasmid contains the human DMD 3’ UTR in place of the SV40 polyA region of the pHRL-CMV vector.

Article Title: Mycobacterium tuberculosis reactivates latent HIV-1 in T cells in vitro
Article Snippet: .. Generation of pNL43-secNLuc2Anef A PCR product containing the secNLuc gene flanked by 5’ NheI and 3’ BamHI restriction sites was generated using PCR primers ( 5’-ccctgctagcgtcttcacactcgaagatttcgttgggg and 5’-cgcgggatcccgccagaatgcgttcgcacagccg ) and pNL1.3[secNLuc ], a plasmid containing the coding sequence for secreted NanoLuc® luciferase (Promega, Cat. #N1021). .. This PCR product and the plasmid pFIN-EF1-GFP-2A-HA-VPR-WPE were digested with NheI and BamHI.

Polymerase Chain Reaction:

Article Title: Conserved regions of the DMD 3’ UTR regulate translation and mRNA abundance in cultured myotubes
Article Snippet: .. The resulting 2,811 bp PCR product was digested with XbaI and AvrII and ligated into the XbaI and BamHI restriction sites of pHRL-CMV (Promega) immediately downstream of the Renilla Luciferase ORF. .. The resulting plasmid contains the human DMD 3’ UTR in place of the SV40 polyA region of the pHRL-CMV vector.

Article Title: Mycobacterium tuberculosis reactivates latent HIV-1 in T cells in vitro
Article Snippet: .. Generation of pNL43-secNLuc2Anef A PCR product containing the secNLuc gene flanked by 5’ NheI and 3’ BamHI restriction sites was generated using PCR primers ( 5’-ccctgctagcgtcttcacactcgaagatttcgttgggg and 5’-cgcgggatcccgccagaatgcgttcgcacagccg ) and pNL1.3[secNLuc ], a plasmid containing the coding sequence for secreted NanoLuc® luciferase (Promega, Cat. #N1021). .. This PCR product and the plasmid pFIN-EF1-GFP-2A-HA-VPR-WPE were digested with NheI and BamHI.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Type IIA Procollagen Containing the Cysteine-rich Amino Propeptide Is Deposited in the Extracellular Matrix of Prechondrogenic Tissue and Binds to TGF-?1 and BMP-2
Article Snippet: .. Primers (5′ primer, 5′-CGTGAATTCCAGGAGGCTGGCAGCTGTGTG-3′; 3′ primer, 5′-GATGGATCCGGCGAGGTCAGTTGGGCAGAT-3′) that flank the exon 2 splice site were used to amplify a 207-bp fragment with EcoRI and BamHI restriction sites from 54-d human fetal embryonic tissue total RNA by using reverse transcription-polymerase chain reaction (RT-PCR), and cloned into pGEM-3zf(+) expression vector ( Promega Corp. ). .. This construct was used to generate antisense and sense riboprobes by in vitro transcription for in situ hybridization.

Generated:

Article Title: Mycobacterium tuberculosis reactivates latent HIV-1 in T cells in vitro
Article Snippet: .. Generation of pNL43-secNLuc2Anef A PCR product containing the secNLuc gene flanked by 5’ NheI and 3’ BamHI restriction sites was generated using PCR primers ( 5’-ccctgctagcgtcttcacactcgaagatttcgttgggg and 5’-cgcgggatcccgccagaatgcgttcgcacagccg ) and pNL1.3[secNLuc ], a plasmid containing the coding sequence for secreted NanoLuc® luciferase (Promega, Cat. #N1021). .. This PCR product and the plasmid pFIN-EF1-GFP-2A-HA-VPR-WPE were digested with NheI and BamHI.

Amplification:

Article Title: INT6 interacts with MIF4GD/SLIP1 and is necessary for efficient histone mRNA translation
Article Snippet: .. The amplified fragments were digested with the XbaI and BamHI restriction enzymes and inserted between the XbaI and BamHI restriction sites of the pGL3 promoter (Promega). .. The promoter sequences of these histone genes were similarly amplified from Jurkat cell DNA using the following primers: H2ACP5′: 5′-GTAAAGATCTGATTTCTGCTACTTATAGGG-3′; H2ACP3′: 5′-TCCAGCCATGGCAATCAGACAAAAATCACC-3′; H2BGP5′: 5′-ATCGGTAGATCTGTGAAAGGCGCAATTTGATTGG-3′; H2BGP3′: 5′-GGTTCAGCCATGGTGTCAGAAAACAATAACAGCAG-3′; H4AP5′: 5′-GCGTGTAGATCTCATCGTCGGAACGGCGCTTCC-3′; H4AP3′: 5′-TGCCGGCCATGGCCGCTGGAGCCCGATAGACAGC-3′.

Plasmid Preparation:

Article Title: Ultrastructural Analysis of ICP34.5− Herpes Simplex Virus 1 Replication in Mouse Brain Cells In Vivo ▿
Article Snippet: .. In a parallel process, pRB4840 containing the α27 promoter flanked by BstYI and BamHI restriction sites was created by ligating the BamHI-EcoRI fragment containing the α27 promoter from pRB3054 into the BglII-EcoRI restriction sites of vector pSP73 (Promega). .. A KpnI site in the polylinker of pSP73 is situated between the EcoRI site and the BamHI site of pSP73. pRB4842 was constructed by insertion of the BstYI-BamHI fragment from pRB4840 containing the α27 promoter into the BamHI site between UL 3 and UL 4 of pRB4841. pRB4843 was constructed by the insertion of a 564-nt KpnI fragment containing the polyadenylation signal from hepatitis B virus from pRB3973 into the KpnI site 3′ of the α27 promoter in the BamHI site between UL 3 and UL 4 in pRB4842.

Article Title: Type IIA Procollagen Containing the Cysteine-rich Amino Propeptide Is Deposited in the Extracellular Matrix of Prechondrogenic Tissue and Binds to TGF-?1 and BMP-2
Article Snippet: .. Primers (5′ primer, 5′-CGTGAATTCCAGGAGGCTGGCAGCTGTGTG-3′; 3′ primer, 5′-GATGGATCCGGCGAGGTCAGTTGGGCAGAT-3′) that flank the exon 2 splice site were used to amplify a 207-bp fragment with EcoRI and BamHI restriction sites from 54-d human fetal embryonic tissue total RNA by using reverse transcription-polymerase chain reaction (RT-PCR), and cloned into pGEM-3zf(+) expression vector ( Promega Corp. ). .. This construct was used to generate antisense and sense riboprobes by in vitro transcription for in situ hybridization.

Article Title: Signaling Lymphocytic Activation Molecule Family Receptor Homologs in New World Monkey Cytomegaloviruses
Article Snippet: .. An sbSLAMF6-Fc fusion protein containing the CD33 leader peptide and the Fc region of human IgG1 was obtained by chemically synthesizing the DNA sequences of the two Ig domains of S. boliviensis SLAMF6 with a splice donor site sequence flanked by BamHI restriction sites and cloning them into the pCI-neo Fc vector. pCI-neo Fc is derived from the pCI-neo vector (Promega) after introduction of the CD33 leader peptide and the Fc region of human IgG1 from the mammalian expression vector signal pIg-Tail (R & D Systems) within the XbaI and NotI restriction sites and disruption of its original BamHI site. .. In sbSLAMF6-Fc, a silent point mutation was included in the chemically synthesized sbSLAMF6 DNA sequence to eliminate the internal BamHI restriction site.

Article Title: Annexin-1-mediated Endothelial Cell Migration and Angiogenesis Are Regulated by Vascular Endothelial Growth Factor (VEGF)-induced Inhibition of miR-196a Expression *
Article Snippet: .. ANXA1 cDNA without 3′-UTR region was subcloned in CSII-Venus vector using NotI and BamHI restriction sites (CSII-Venus-ANXA1). psiCHECK-2 vector was obtained from Promega (Madison, WI). .. ANXA1 coding sequence and 3′-UTR region was amplified from HUVECs total RNA following cDNA production using the following primers: Forward primer 5′-CTTTGCAAGAAGGTAGAGATAAAGACAC-3′; Reverse primer 5′-CTTGTGACGTCATTTTATTTTCAGCTACATAG-3′ and KOD Hot Start DNA polymerase according to the manufacturer protocol (EMD, Philadelphia, PA).

Article Title: Mycobacterium tuberculosis reactivates latent HIV-1 in T cells in vitro
Article Snippet: .. Generation of pNL43-secNLuc2Anef A PCR product containing the secNLuc gene flanked by 5’ NheI and 3’ BamHI restriction sites was generated using PCR primers ( 5’-ccctgctagcgtcttcacactcgaagatttcgttgggg and 5’-cgcgggatcccgccagaatgcgttcgcacagccg ) and pNL1.3[secNLuc ], a plasmid containing the coding sequence for secreted NanoLuc® luciferase (Promega, Cat. #N1021). .. This PCR product and the plasmid pFIN-EF1-GFP-2A-HA-VPR-WPE were digested with NheI and BamHI.

Expressing:

Article Title: Type IIA Procollagen Containing the Cysteine-rich Amino Propeptide Is Deposited in the Extracellular Matrix of Prechondrogenic Tissue and Binds to TGF-?1 and BMP-2
Article Snippet: .. Primers (5′ primer, 5′-CGTGAATTCCAGGAGGCTGGCAGCTGTGTG-3′; 3′ primer, 5′-GATGGATCCGGCGAGGTCAGTTGGGCAGAT-3′) that flank the exon 2 splice site were used to amplify a 207-bp fragment with EcoRI and BamHI restriction sites from 54-d human fetal embryonic tissue total RNA by using reverse transcription-polymerase chain reaction (RT-PCR), and cloned into pGEM-3zf(+) expression vector ( Promega Corp. ). .. This construct was used to generate antisense and sense riboprobes by in vitro transcription for in situ hybridization.

Article Title: Signaling Lymphocytic Activation Molecule Family Receptor Homologs in New World Monkey Cytomegaloviruses
Article Snippet: .. An sbSLAMF6-Fc fusion protein containing the CD33 leader peptide and the Fc region of human IgG1 was obtained by chemically synthesizing the DNA sequences of the two Ig domains of S. boliviensis SLAMF6 with a splice donor site sequence flanked by BamHI restriction sites and cloning them into the pCI-neo Fc vector. pCI-neo Fc is derived from the pCI-neo vector (Promega) after introduction of the CD33 leader peptide and the Fc region of human IgG1 from the mammalian expression vector signal pIg-Tail (R & D Systems) within the XbaI and NotI restriction sites and disruption of its original BamHI site. .. In sbSLAMF6-Fc, a silent point mutation was included in the chemically synthesized sbSLAMF6 DNA sequence to eliminate the internal BamHI restriction site.

Sequencing:

Article Title: Signaling Lymphocytic Activation Molecule Family Receptor Homologs in New World Monkey Cytomegaloviruses
Article Snippet: .. An sbSLAMF6-Fc fusion protein containing the CD33 leader peptide and the Fc region of human IgG1 was obtained by chemically synthesizing the DNA sequences of the two Ig domains of S. boliviensis SLAMF6 with a splice donor site sequence flanked by BamHI restriction sites and cloning them into the pCI-neo Fc vector. pCI-neo Fc is derived from the pCI-neo vector (Promega) after introduction of the CD33 leader peptide and the Fc region of human IgG1 from the mammalian expression vector signal pIg-Tail (R & D Systems) within the XbaI and NotI restriction sites and disruption of its original BamHI site. .. In sbSLAMF6-Fc, a silent point mutation was included in the chemically synthesized sbSLAMF6 DNA sequence to eliminate the internal BamHI restriction site.

Article Title: Mycobacterium tuberculosis reactivates latent HIV-1 in T cells in vitro
Article Snippet: .. Generation of pNL43-secNLuc2Anef A PCR product containing the secNLuc gene flanked by 5’ NheI and 3’ BamHI restriction sites was generated using PCR primers ( 5’-ccctgctagcgtcttcacactcgaagatttcgttgggg and 5’-cgcgggatcccgccagaatgcgttcgcacagccg ) and pNL1.3[secNLuc ], a plasmid containing the coding sequence for secreted NanoLuc® luciferase (Promega, Cat. #N1021). .. This PCR product and the plasmid pFIN-EF1-GFP-2A-HA-VPR-WPE were digested with NheI and BamHI.

Derivative Assay:

Article Title: Signaling Lymphocytic Activation Molecule Family Receptor Homologs in New World Monkey Cytomegaloviruses
Article Snippet: .. An sbSLAMF6-Fc fusion protein containing the CD33 leader peptide and the Fc region of human IgG1 was obtained by chemically synthesizing the DNA sequences of the two Ig domains of S. boliviensis SLAMF6 with a splice donor site sequence flanked by BamHI restriction sites and cloning them into the pCI-neo Fc vector. pCI-neo Fc is derived from the pCI-neo vector (Promega) after introduction of the CD33 leader peptide and the Fc region of human IgG1 from the mammalian expression vector signal pIg-Tail (R & D Systems) within the XbaI and NotI restriction sites and disruption of its original BamHI site. .. In sbSLAMF6-Fc, a silent point mutation was included in the chemically synthesized sbSLAMF6 DNA sequence to eliminate the internal BamHI restriction site.

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    Promega bamhi restriction sites
    Bamhi Restriction Sites, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi restriction sites/product/Promega
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bamhi restriction sites - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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