bamhi restriction sites  (Millipore)


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    Structured Review

    Millipore bamhi restriction sites
    Bamhi Restriction Sites, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi restriction sites/product/Millipore
    Average 93 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    bamhi restriction sites - by Bioz Stars, 2020-07
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Expression, refolding, and initial structural characterization of the Y. pestis Ail outer membrane protein in lipids
    Article Snippet: .. The gene encoding mature Ail from Y. pestis KIM 10 (gene y1324, without the signal sequence) was cloned in the NdeI and BamHI restriction sites of the E. coli plasmid vector pET-3b (Novagen). .. Deletion of the signal sequence directs the expression of Ail into inclusion bodies.

    Article Title: Single Point Mutation in Bin/Amphiphysin/Rvs (BAR) Sequence of Endophilin Impairs Dimerization, Membrane Shaping, and Src Homology 3 Domain-mediated Partnership *
    Article Snippet: .. cDNAs encoding for mouse EA1 and the deletion mutants EA1Δ(1–97), EA1Δ(1–175), EA1Δ(1–200), and EA1varSH3 were amplified using sense and antisense primers designed to contain BamHI restriction sites for cloning into the bacterial pET9a expression vector (Novagen). .. EA1(1/2var)SH3 and EA1SH3 were cloned into pET9a using primers introducing a sequence encoding for a StrepTag in the N terminus and containing NdeI/BamHI restriction sites.

    Article Title: The Structure of the Dust Mite Allergen Der p 7 Reveals Similarities to Innate Immune Proteins
    Article Snippet: .. DNA encoding residues 18-215 of Der p 7 representing the native expressed mite protein was cloned into a modified fixed-arm PMAL vector between the NotI and BamHI restriction sites with MBP mutations D82A/K83A/E172A/N173A/K239A., The MBP-Der p 7 fusion protein was expressed in E. coli Rosetta2(DE3) pLacI cells (Novagen), and purified using the amylose affinity resin in batch followed by gel-filtration utilizing a 16/60 superdex 200 column and ionic exchang139 e chromatography step using Q sepharose. .. The purified protein was dialyzed against 25 mM HEPES pH 7.4, 75 mM NaCl, 5 mM maltose and concentrated to 27 mg/mL.

    Amplification:

    Article Title: Structural and functional characterization of the TYW3/Taw3 class of SAM-dependent methyltransferases
    Article Snippet: .. The open reading frame of the SSO0622 gene from Sulfolobus solfataricus was PCR amplified and inserted between the NdeI and BamHI restriction sites of a modified pET-15b expression vector (Novagen) (p11) as previously described ( ). .. This construct generated an N-terminal hexahistidine tag joined to the Ss Taw3 protein by the TEV protease recognition site (ENLYFQ↓G).

    Article Title: Single Point Mutation in Bin/Amphiphysin/Rvs (BAR) Sequence of Endophilin Impairs Dimerization, Membrane Shaping, and Src Homology 3 Domain-mediated Partnership *
    Article Snippet: .. cDNAs encoding for mouse EA1 and the deletion mutants EA1Δ(1–97), EA1Δ(1–175), EA1Δ(1–200), and EA1varSH3 were amplified using sense and antisense primers designed to contain BamHI restriction sites for cloning into the bacterial pET9a expression vector (Novagen). .. EA1(1/2var)SH3 and EA1SH3 were cloned into pET9a using primers introducing a sequence encoding for a StrepTag in the N terminus and containing NdeI/BamHI restriction sites.

    Article Title: Syntrophomonas wolfei Uses an NADH-Dependent, Ferredoxin-Independent [FeFe]-Hydrogenase To Reoxidize NADH
    Article Snippet: .. The Hyd maturases HydE (SWOL_RS05180) and HydG (SWOL_RS05190), as well as a 348-bp open reading frame with no identifiable conserved domains (SWOL_RS05185), were amplified from the S. wolfei genomic template with the addition of NcoI and BamHI restriction sites and were inserted into the MCS of pCDFDuet-1 (Novagen, Merck KGaA, Darmstadt, Germany) (Fig. S1). .. In addition, the gene encoding the Hyd maturase HydF (SWOL_RS01625) was amplified with restriction sites for NdeI and XhoI and inserted into the second MCS of pCDFDuet-1 to generate pCDFDuet-1 SwHydEFG.

    Article Title:
    Article Snippet: .. This PBP amplicon served as a template to generate the product coding for TC-1, with primers containing NdeI and BamHI restriction sites to allow ligation of the digested PCR product to NdeI-BamHI-digested pET9a expression vector (Novagen). ..

    Chromatography:

    Article Title: The Structure of the Dust Mite Allergen Der p 7 Reveals Similarities to Innate Immune Proteins
    Article Snippet: .. DNA encoding residues 18-215 of Der p 7 representing the native expressed mite protein was cloned into a modified fixed-arm PMAL vector between the NotI and BamHI restriction sites with MBP mutations D82A/K83A/E172A/N173A/K239A., The MBP-Der p 7 fusion protein was expressed in E. coli Rosetta2(DE3) pLacI cells (Novagen), and purified using the amylose affinity resin in batch followed by gel-filtration utilizing a 16/60 superdex 200 column and ionic exchang139 e chromatography step using Q sepharose. .. The purified protein was dialyzed against 25 mM HEPES pH 7.4, 75 mM NaCl, 5 mM maltose and concentrated to 27 mg/mL.

    Ligation:

    Article Title:
    Article Snippet: .. This PBP amplicon served as a template to generate the product coding for TC-1, with primers containing NdeI and BamHI restriction sites to allow ligation of the digested PCR product to NdeI-BamHI-digested pET9a expression vector (Novagen). ..

    Purification:

    Article Title: The Structure of the Dust Mite Allergen Der p 7 Reveals Similarities to Innate Immune Proteins
    Article Snippet: .. DNA encoding residues 18-215 of Der p 7 representing the native expressed mite protein was cloned into a modified fixed-arm PMAL vector between the NotI and BamHI restriction sites with MBP mutations D82A/K83A/E172A/N173A/K239A., The MBP-Der p 7 fusion protein was expressed in E. coli Rosetta2(DE3) pLacI cells (Novagen), and purified using the amylose affinity resin in batch followed by gel-filtration utilizing a 16/60 superdex 200 column and ionic exchang139 e chromatography step using Q sepharose. .. The purified protein was dialyzed against 25 mM HEPES pH 7.4, 75 mM NaCl, 5 mM maltose and concentrated to 27 mg/mL.

    Sequencing:

    Article Title: Expression, refolding, and initial structural characterization of the Y. pestis Ail outer membrane protein in lipids
    Article Snippet: .. The gene encoding mature Ail from Y. pestis KIM 10 (gene y1324, without the signal sequence) was cloned in the NdeI and BamHI restriction sites of the E. coli plasmid vector pET-3b (Novagen). .. Deletion of the signal sequence directs the expression of Ail into inclusion bodies.

    Polymerase Chain Reaction:

    Article Title: Structural and functional characterization of the TYW3/Taw3 class of SAM-dependent methyltransferases
    Article Snippet: .. The open reading frame of the SSO0622 gene from Sulfolobus solfataricus was PCR amplified and inserted between the NdeI and BamHI restriction sites of a modified pET-15b expression vector (Novagen) (p11) as previously described ( ). .. This construct generated an N-terminal hexahistidine tag joined to the Ss Taw3 protein by the TEV protease recognition site (ENLYFQ↓G).

    Article Title: A Revised Pathway Proposed for Staphylococcus aureus Wall Teichoic Acid Biosynthesis Based on In Vitro Reconstitution of the Intracellular Steps
    Article Snippet: .. The PCR products were subcloned into pET24b(+) (Novagen) at the XhoI and BamHI restriction sites for expression in E. coli Rosetta2(DE3)pLysS (Novagen) as C-terminal hexa-His-tagged proteins. ..

    Article Title:
    Article Snippet: .. This PBP amplicon served as a template to generate the product coding for TC-1, with primers containing NdeI and BamHI restriction sites to allow ligation of the digested PCR product to NdeI-BamHI-digested pET9a expression vector (Novagen). ..

    Positron Emission Tomography:

    Article Title: Expression, refolding, and initial structural characterization of the Y. pestis Ail outer membrane protein in lipids
    Article Snippet: .. The gene encoding mature Ail from Y. pestis KIM 10 (gene y1324, without the signal sequence) was cloned in the NdeI and BamHI restriction sites of the E. coli plasmid vector pET-3b (Novagen). .. Deletion of the signal sequence directs the expression of Ail into inclusion bodies.

    Article Title: Altered architecture of substrate binding region defines the unique specificity of UDP-GalNAc 4-epimerases
    Article Snippet: .. Briefly, wbgU was inserted between XhoI and BamHI restriction sites of pET-15b plasmid (Novagen) with an N-terminal (His)6 fusion tag. .. The construct pET/wbgU was transformed into BL21(DE3) (Novagen) for protein expression.

    Article Title: Structural and functional characterization of the TYW3/Taw3 class of SAM-dependent methyltransferases
    Article Snippet: .. The open reading frame of the SSO0622 gene from Sulfolobus solfataricus was PCR amplified and inserted between the NdeI and BamHI restriction sites of a modified pET-15b expression vector (Novagen) (p11) as previously described ( ). .. This construct generated an N-terminal hexahistidine tag joined to the Ss Taw3 protein by the TEV protease recognition site (ENLYFQ↓G).

    Expressing:

    Article Title: Structural and functional characterization of the TYW3/Taw3 class of SAM-dependent methyltransferases
    Article Snippet: .. The open reading frame of the SSO0622 gene from Sulfolobus solfataricus was PCR amplified and inserted between the NdeI and BamHI restriction sites of a modified pET-15b expression vector (Novagen) (p11) as previously described ( ). .. This construct generated an N-terminal hexahistidine tag joined to the Ss Taw3 protein by the TEV protease recognition site (ENLYFQ↓G).

    Article Title: Single Point Mutation in Bin/Amphiphysin/Rvs (BAR) Sequence of Endophilin Impairs Dimerization, Membrane Shaping, and Src Homology 3 Domain-mediated Partnership *
    Article Snippet: .. cDNAs encoding for mouse EA1 and the deletion mutants EA1Δ(1–97), EA1Δ(1–175), EA1Δ(1–200), and EA1varSH3 were amplified using sense and antisense primers designed to contain BamHI restriction sites for cloning into the bacterial pET9a expression vector (Novagen). .. EA1(1/2var)SH3 and EA1SH3 were cloned into pET9a using primers introducing a sequence encoding for a StrepTag in the N terminus and containing NdeI/BamHI restriction sites.

    Article Title: A Revised Pathway Proposed for Staphylococcus aureus Wall Teichoic Acid Biosynthesis Based on In Vitro Reconstitution of the Intracellular Steps
    Article Snippet: .. The PCR products were subcloned into pET24b(+) (Novagen) at the XhoI and BamHI restriction sites for expression in E. coli Rosetta2(DE3)pLysS (Novagen) as C-terminal hexa-His-tagged proteins. ..

    Article Title:
    Article Snippet: .. This PBP amplicon served as a template to generate the product coding for TC-1, with primers containing NdeI and BamHI restriction sites to allow ligation of the digested PCR product to NdeI-BamHI-digested pET9a expression vector (Novagen). ..

    Modification:

    Article Title: Structural and functional characterization of the TYW3/Taw3 class of SAM-dependent methyltransferases
    Article Snippet: .. The open reading frame of the SSO0622 gene from Sulfolobus solfataricus was PCR amplified and inserted between the NdeI and BamHI restriction sites of a modified pET-15b expression vector (Novagen) (p11) as previously described ( ). .. This construct generated an N-terminal hexahistidine tag joined to the Ss Taw3 protein by the TEV protease recognition site (ENLYFQ↓G).

    Article Title: The Structure of the Dust Mite Allergen Der p 7 Reveals Similarities to Innate Immune Proteins
    Article Snippet: .. DNA encoding residues 18-215 of Der p 7 representing the native expressed mite protein was cloned into a modified fixed-arm PMAL vector between the NotI and BamHI restriction sites with MBP mutations D82A/K83A/E172A/N173A/K239A., The MBP-Der p 7 fusion protein was expressed in E. coli Rosetta2(DE3) pLacI cells (Novagen), and purified using the amylose affinity resin in batch followed by gel-filtration utilizing a 16/60 superdex 200 column and ionic exchang139 e chromatography step using Q sepharose. .. The purified protein was dialyzed against 25 mM HEPES pH 7.4, 75 mM NaCl, 5 mM maltose and concentrated to 27 mg/mL.

    Plasmid Preparation:

    Article Title: Expression, refolding, and initial structural characterization of the Y. pestis Ail outer membrane protein in lipids
    Article Snippet: .. The gene encoding mature Ail from Y. pestis KIM 10 (gene y1324, without the signal sequence) was cloned in the NdeI and BamHI restriction sites of the E. coli plasmid vector pET-3b (Novagen). .. Deletion of the signal sequence directs the expression of Ail into inclusion bodies.

    Article Title: Altered architecture of substrate binding region defines the unique specificity of UDP-GalNAc 4-epimerases
    Article Snippet: .. Briefly, wbgU was inserted between XhoI and BamHI restriction sites of pET-15b plasmid (Novagen) with an N-terminal (His)6 fusion tag. .. The construct pET/wbgU was transformed into BL21(DE3) (Novagen) for protein expression.

    Article Title: Structural and functional characterization of the TYW3/Taw3 class of SAM-dependent methyltransferases
    Article Snippet: .. The open reading frame of the SSO0622 gene from Sulfolobus solfataricus was PCR amplified and inserted between the NdeI and BamHI restriction sites of a modified pET-15b expression vector (Novagen) (p11) as previously described ( ). .. This construct generated an N-terminal hexahistidine tag joined to the Ss Taw3 protein by the TEV protease recognition site (ENLYFQ↓G).

    Article Title: Single Point Mutation in Bin/Amphiphysin/Rvs (BAR) Sequence of Endophilin Impairs Dimerization, Membrane Shaping, and Src Homology 3 Domain-mediated Partnership *
    Article Snippet: .. cDNAs encoding for mouse EA1 and the deletion mutants EA1Δ(1–97), EA1Δ(1–175), EA1Δ(1–200), and EA1varSH3 were amplified using sense and antisense primers designed to contain BamHI restriction sites for cloning into the bacterial pET9a expression vector (Novagen). .. EA1(1/2var)SH3 and EA1SH3 were cloned into pET9a using primers introducing a sequence encoding for a StrepTag in the N terminus and containing NdeI/BamHI restriction sites.

    Article Title: The Structure of the Dust Mite Allergen Der p 7 Reveals Similarities to Innate Immune Proteins
    Article Snippet: .. DNA encoding residues 18-215 of Der p 7 representing the native expressed mite protein was cloned into a modified fixed-arm PMAL vector between the NotI and BamHI restriction sites with MBP mutations D82A/K83A/E172A/N173A/K239A., The MBP-Der p 7 fusion protein was expressed in E. coli Rosetta2(DE3) pLacI cells (Novagen), and purified using the amylose affinity resin in batch followed by gel-filtration utilizing a 16/60 superdex 200 column and ionic exchang139 e chromatography step using Q sepharose. .. The purified protein was dialyzed against 25 mM HEPES pH 7.4, 75 mM NaCl, 5 mM maltose and concentrated to 27 mg/mL.

    Article Title:
    Article Snippet: .. This PBP amplicon served as a template to generate the product coding for TC-1, with primers containing NdeI and BamHI restriction sites to allow ligation of the digested PCR product to NdeI-BamHI-digested pET9a expression vector (Novagen). ..

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  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
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  • 93
    Millipore bamhi restriction sites
    Bamhi Restriction Sites, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi restriction sites/product/Millipore
    Average 93 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    bamhi restriction sites - by Bioz Stars, 2020-07
    93/100 stars
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