Structured Review

GenScript bamhi restriction sites
Bamhi Restriction Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi restriction sites/product/GenScript
Average 92 stars, based on 10 article reviews
Price from $9.99 to $1999.99
bamhi restriction sites - by Bioz Stars, 2020-07
92/100 stars

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Clone Assay:

Article Title: Calcium is an essential cofactor for metal efflux by the ferroportin transporter family
Article Snippet: .. The gene for BbFpn with flanking XhoI and BamHI restriction sites was purchased from GenScript (USA) and cloned into pWaldoE , generating a C-terminal fusion with GFP-His8 and intermittent tobacco etch virus (TEV) protease cleavage site. .. Protein was expressed in E. coli C41 cells (G. von Heijne, Stockholm University) grown in terrific broth (TB) at 37 °C until OD600 reached 0.8, after which the temperature was reduced to 20 °C.

Article Title: The X-ray structure of human calbindin-D28K: an improved model
Article Snippet: .. Cloning and expression The Escherichia coli codon-optimized human CALB1 gene was synthesized and cloned into pET-15b using NcoI and BamHI restriction sites (GenScript). .. A polyhistidine tag followed by a rhinovirus (HRV) 3C protease cleavage sequence was included in the synthetic construct directly 5′ to the calbindin-D28K start codon.

Article Title: Localization and functional characterization of the pathogenesis-related proteins Rbe1p and Rbt4p in Candida albicans
Article Snippet: .. Escherichia coli For heterologous expression of Rbe1p and Rbt4p in E . coli , RBE1 and RBT4 gene sequences were codon optimized , chemically synthesized with additional XhoI and BamHI restriction sites and cloned into vector pET-19b(+) (GenScript, Piscataway, USA). .. Transformation of SHuffle® T7 Competent E . coli cells (New England Biolabs) with the plasmids was done using the method described by Chung et al .

Article Title: Cryo-EM structure of the bacteria killing type IV secretion system core complex from Xanthomonas citri
Article Snippet: .. The DNA sequence encoding VirB10NTD (residues 85 to 182) was chemically synthetized with codon optimization for expression in E. coli and cloned into the expression vector pET28a between NdeI and BamHI restriction sites (GenScript). .. Uniformly 15 N/13 C doubled labeled His-VirB10NTD was expressed in E. coli BL21(DE3) RP cells in M9 minimal medium containing 1.0 g/L of 15 NH4 Cl and 2.0 g/L of 13 C-glucose as the only nitrogen and carbon sources, respectively.

Article Title: Calcium is an essential cofactor for metal efflux by the ferroportin transporter family
Article Snippet: .. BbFpn gene cloning and protein expression The gene for BbFpn with flanking XhoI and BamHI restriction sites was purchased from GenScript (USA) and cloned into pWaldoE , generating a C-terminal fusion with GFP-His8 and intermittent tobacco etch virus (TEV) protease cleavage site. .. Protein was expressed in E. coli C41 cells (G. von Heijne, Stockholm University) grown in terrific broth (TB) at 37 °C until OD600 reached 0.8, after which the temperature was reduced to 20 °C.

Article Title: Localization and functional characterization of the pathogenesis-related proteins Rbe1p and Rbt4p in Candida albicans
Article Snippet: .. For heterologous expression of Rbe1p and Rbt4p in E . coli , RBE1 and RBT4 gene sequences were codon optimized , chemically synthesized with additional XhoI and BamHI restriction sites and cloned into vector pET-19b(+) (GenScript, Piscataway, USA). .. Transformation of SHuffle® T7 Competent E . coli cells (New England Biolabs) with the plasmids was done using the method described by Chung et al .

Positron Emission Tomography:

Article Title: The X-ray structure of human calbindin-D28K: an improved model
Article Snippet: .. Cloning and expression The Escherichia coli codon-optimized human CALB1 gene was synthesized and cloned into pET-15b using NcoI and BamHI restriction sites (GenScript). .. A polyhistidine tag followed by a rhinovirus (HRV) 3C protease cleavage sequence was included in the synthetic construct directly 5′ to the calbindin-D28K start codon.

Article Title: Localization and functional characterization of the pathogenesis-related proteins Rbe1p and Rbt4p in Candida albicans
Article Snippet: .. Escherichia coli For heterologous expression of Rbe1p and Rbt4p in E . coli , RBE1 and RBT4 gene sequences were codon optimized , chemically synthesized with additional XhoI and BamHI restriction sites and cloned into vector pET-19b(+) (GenScript, Piscataway, USA). .. Transformation of SHuffle® T7 Competent E . coli cells (New England Biolabs) with the plasmids was done using the method described by Chung et al .

Article Title: Localization and functional characterization of the pathogenesis-related proteins Rbe1p and Rbt4p in Candida albicans
Article Snippet: .. For heterologous expression of Rbe1p and Rbt4p in E . coli , RBE1 and RBT4 gene sequences were codon optimized , chemically synthesized with additional XhoI and BamHI restriction sites and cloned into vector pET-19b(+) (GenScript, Piscataway, USA). .. Transformation of SHuffle® T7 Competent E . coli cells (New England Biolabs) with the plasmids was done using the method described by Chung et al .

Synthesized:

Article Title: The X-ray structure of human calbindin-D28K: an improved model
Article Snippet: .. Cloning and expression The Escherichia coli codon-optimized human CALB1 gene was synthesized and cloned into pET-15b using NcoI and BamHI restriction sites (GenScript). .. A polyhistidine tag followed by a rhinovirus (HRV) 3C protease cleavage sequence was included in the synthetic construct directly 5′ to the calbindin-D28K start codon.

Article Title: Localization and functional characterization of the pathogenesis-related proteins Rbe1p and Rbt4p in Candida albicans
Article Snippet: .. Escherichia coli For heterologous expression of Rbe1p and Rbt4p in E . coli , RBE1 and RBT4 gene sequences were codon optimized , chemically synthesized with additional XhoI and BamHI restriction sites and cloned into vector pET-19b(+) (GenScript, Piscataway, USA). .. Transformation of SHuffle® T7 Competent E . coli cells (New England Biolabs) with the plasmids was done using the method described by Chung et al .

Article Title: Detection of distinct glycosylation patterns on human γ-glutamyl transpeptidase 1 using antibody-lectin sandwich array (ALSA) technology
Article Snippet: .. Microvirin purification The nucleotide sequence for the open-reading frame (ORF) of MVN (EMBL accession AM041066) was synthesized with flanking NdeI and BamHI restriction sites in the pUC57 shuttle vector GenScript (Piscataway, NJ). .. The MVN ORF was transferred into the E. coli expression construct pET-15b (Invitrogen, Grand Island, NY), using NdeI and BamHI, where it was expected to encode the full-length protein with an N-terminal hexahistadine tag.

Article Title: Divergence of Erv1-Associated Mitochondrial Import and Export Pathways in Trypanosomes and Anaerobic Protists
Article Snippet: .. An ORF flanked by NdeI and BamHI restriction sites was synthesized (Genscript), restriction digested, and ligated into pET14b that had also been digested with NdeI and BamHI. .. Protein (TbTIM9) was expressed in E. coli CD43-DE3 cells and purified from cell extracts using the N-terminal polyhistidine tag.

Article Title: Localization and functional characterization of the pathogenesis-related proteins Rbe1p and Rbt4p in Candida albicans
Article Snippet: .. For heterologous expression of Rbe1p and Rbt4p in E . coli , RBE1 and RBT4 gene sequences were codon optimized , chemically synthesized with additional XhoI and BamHI restriction sites and cloned into vector pET-19b(+) (GenScript, Piscataway, USA). .. Transformation of SHuffle® T7 Competent E . coli cells (New England Biolabs) with the plasmids was done using the method described by Chung et al .

Purification:

Article Title: Detection of distinct glycosylation patterns on human γ-glutamyl transpeptidase 1 using antibody-lectin sandwich array (ALSA) technology
Article Snippet: .. Microvirin purification The nucleotide sequence for the open-reading frame (ORF) of MVN (EMBL accession AM041066) was synthesized with flanking NdeI and BamHI restriction sites in the pUC57 shuttle vector GenScript (Piscataway, NJ). .. The MVN ORF was transferred into the E. coli expression construct pET-15b (Invitrogen, Grand Island, NY), using NdeI and BamHI, where it was expected to encode the full-length protein with an N-terminal hexahistadine tag.

Expressing:

Article Title: The X-ray structure of human calbindin-D28K: an improved model
Article Snippet: .. Cloning and expression The Escherichia coli codon-optimized human CALB1 gene was synthesized and cloned into pET-15b using NcoI and BamHI restriction sites (GenScript). .. A polyhistidine tag followed by a rhinovirus (HRV) 3C protease cleavage sequence was included in the synthetic construct directly 5′ to the calbindin-D28K start codon.

Article Title: Localization and functional characterization of the pathogenesis-related proteins Rbe1p and Rbt4p in Candida albicans
Article Snippet: .. Escherichia coli For heterologous expression of Rbe1p and Rbt4p in E . coli , RBE1 and RBT4 gene sequences were codon optimized , chemically synthesized with additional XhoI and BamHI restriction sites and cloned into vector pET-19b(+) (GenScript, Piscataway, USA). .. Transformation of SHuffle® T7 Competent E . coli cells (New England Biolabs) with the plasmids was done using the method described by Chung et al .

Article Title: Cryo-EM structure of the bacteria killing type IV secretion system core complex from Xanthomonas citri
Article Snippet: .. The DNA sequence encoding VirB10NTD (residues 85 to 182) was chemically synthetized with codon optimization for expression in E. coli and cloned into the expression vector pET28a between NdeI and BamHI restriction sites (GenScript). .. Uniformly 15 N/13 C doubled labeled His-VirB10NTD was expressed in E. coli BL21(DE3) RP cells in M9 minimal medium containing 1.0 g/L of 15 NH4 Cl and 2.0 g/L of 13 C-glucose as the only nitrogen and carbon sources, respectively.

Article Title: Calcium is an essential cofactor for metal efflux by the ferroportin transporter family
Article Snippet: .. BbFpn gene cloning and protein expression The gene for BbFpn with flanking XhoI and BamHI restriction sites was purchased from GenScript (USA) and cloned into pWaldoE , generating a C-terminal fusion with GFP-His8 and intermittent tobacco etch virus (TEV) protease cleavage site. .. Protein was expressed in E. coli C41 cells (G. von Heijne, Stockholm University) grown in terrific broth (TB) at 37 °C until OD600 reached 0.8, after which the temperature was reduced to 20 °C.

Article Title: Localization and functional characterization of the pathogenesis-related proteins Rbe1p and Rbt4p in Candida albicans
Article Snippet: .. For heterologous expression of Rbe1p and Rbt4p in E . coli , RBE1 and RBT4 gene sequences were codon optimized , chemically synthesized with additional XhoI and BamHI restriction sites and cloned into vector pET-19b(+) (GenScript, Piscataway, USA). .. Transformation of SHuffle® T7 Competent E . coli cells (New England Biolabs) with the plasmids was done using the method described by Chung et al .

Sequencing:

Article Title: Cryo-EM structure of the bacteria killing type IV secretion system core complex from Xanthomonas citri
Article Snippet: .. The DNA sequence encoding VirB10NTD (residues 85 to 182) was chemically synthetized with codon optimization for expression in E. coli and cloned into the expression vector pET28a between NdeI and BamHI restriction sites (GenScript). .. Uniformly 15 N/13 C doubled labeled His-VirB10NTD was expressed in E. coli BL21(DE3) RP cells in M9 minimal medium containing 1.0 g/L of 15 NH4 Cl and 2.0 g/L of 13 C-glucose as the only nitrogen and carbon sources, respectively.

Article Title: Detection of distinct glycosylation patterns on human γ-glutamyl transpeptidase 1 using antibody-lectin sandwich array (ALSA) technology
Article Snippet: .. Microvirin purification The nucleotide sequence for the open-reading frame (ORF) of MVN (EMBL accession AM041066) was synthesized with flanking NdeI and BamHI restriction sites in the pUC57 shuttle vector GenScript (Piscataway, NJ). .. The MVN ORF was transferred into the E. coli expression construct pET-15b (Invitrogen, Grand Island, NY), using NdeI and BamHI, where it was expected to encode the full-length protein with an N-terminal hexahistadine tag.

Plasmid Preparation:

Article Title: Localization and functional characterization of the pathogenesis-related proteins Rbe1p and Rbt4p in Candida albicans
Article Snippet: .. Escherichia coli For heterologous expression of Rbe1p and Rbt4p in E . coli , RBE1 and RBT4 gene sequences were codon optimized , chemically synthesized with additional XhoI and BamHI restriction sites and cloned into vector pET-19b(+) (GenScript, Piscataway, USA). .. Transformation of SHuffle® T7 Competent E . coli cells (New England Biolabs) with the plasmids was done using the method described by Chung et al .

Article Title: Cryo-EM structure of the bacteria killing type IV secretion system core complex from Xanthomonas citri
Article Snippet: .. The DNA sequence encoding VirB10NTD (residues 85 to 182) was chemically synthetized with codon optimization for expression in E. coli and cloned into the expression vector pET28a between NdeI and BamHI restriction sites (GenScript). .. Uniformly 15 N/13 C doubled labeled His-VirB10NTD was expressed in E. coli BL21(DE3) RP cells in M9 minimal medium containing 1.0 g/L of 15 NH4 Cl and 2.0 g/L of 13 C-glucose as the only nitrogen and carbon sources, respectively.

Article Title: Detection of distinct glycosylation patterns on human γ-glutamyl transpeptidase 1 using antibody-lectin sandwich array (ALSA) technology
Article Snippet: .. Microvirin purification The nucleotide sequence for the open-reading frame (ORF) of MVN (EMBL accession AM041066) was synthesized with flanking NdeI and BamHI restriction sites in the pUC57 shuttle vector GenScript (Piscataway, NJ). .. The MVN ORF was transferred into the E. coli expression construct pET-15b (Invitrogen, Grand Island, NY), using NdeI and BamHI, where it was expected to encode the full-length protein with an N-terminal hexahistadine tag.

Article Title: Localization and functional characterization of the pathogenesis-related proteins Rbe1p and Rbt4p in Candida albicans
Article Snippet: .. For heterologous expression of Rbe1p and Rbt4p in E . coli , RBE1 and RBT4 gene sequences were codon optimized , chemically synthesized with additional XhoI and BamHI restriction sites and cloned into vector pET-19b(+) (GenScript, Piscataway, USA). .. Transformation of SHuffle® T7 Competent E . coli cells (New England Biolabs) with the plasmids was done using the method described by Chung et al .

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    GenScript bamh1 restriction sites
    Bamh1 Restriction Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamh1 restriction sites/product/GenScript
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bamh1 restriction sites - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    92
    GenScript bamhi restriction sites
    Bamhi Restriction Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi restriction sites/product/GenScript
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    bamhi restriction sites - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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