Structured Review

GenScript bamhi 3 xhoi sites
Bamhi 3 Xhoi Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi 3 xhoi sites/product/GenScript
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
bamhi 3 xhoi sites - by Bioz Stars, 2020-07
91/100 stars

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Related Articles

Sequencing:

Article Title: Elucidation of MRAS-mediated Noonan syndrome with cardiac hypertrophy
Article Snippet: .. A pcDNA3.1+C-HA vector customized with MRAS cDNA subcloned in at 5′BamHI-3′XhoI sites was purchased from GenScript. cDNA sequence was verified by sequencing. p.Gly23Val-MRAS missense mutations were engineered into pcDNA3.1- MRAS -C-HA vector using primers containing the missense mutations with the Quikchange II XL Site-Directed Mutagenesis Kit (Stratagene). .. Primary antibodies were used at the following dilutions: anti-phospho-ERK1/2 (1:1,000, Cell Signaling 9101) in 3% BSA, anti-ERK1/2 (1:1,000, Cell Signaling 9102) in 3% BSA, anti-HA tag (1:1,000, Santa Cruz Biotechnology SC-7392) in 3% BSA, and β-actin (1:5,000, Sigma-Aldrich A2228) in 5% nonfat dried milk.

Mutagenesis:

Article Title: Elucidation of MRAS-mediated Noonan syndrome with cardiac hypertrophy
Article Snippet: .. A pcDNA3.1+C-HA vector customized with MRAS cDNA subcloned in at 5′BamHI-3′XhoI sites was purchased from GenScript. cDNA sequence was verified by sequencing. p.Gly23Val-MRAS missense mutations were engineered into pcDNA3.1- MRAS -C-HA vector using primers containing the missense mutations with the Quikchange II XL Site-Directed Mutagenesis Kit (Stratagene). .. Primary antibodies were used at the following dilutions: anti-phospho-ERK1/2 (1:1,000, Cell Signaling 9101) in 3% BSA, anti-ERK1/2 (1:1,000, Cell Signaling 9102) in 3% BSA, anti-HA tag (1:1,000, Santa Cruz Biotechnology SC-7392) in 3% BSA, and β-actin (1:5,000, Sigma-Aldrich A2228) in 5% nonfat dried milk.

Plasmid Preparation:

Article Title: Elucidation of MRAS-mediated Noonan syndrome with cardiac hypertrophy
Article Snippet: .. A pcDNA3.1+C-HA vector customized with MRAS cDNA subcloned in at 5′BamHI-3′XhoI sites was purchased from GenScript. cDNA sequence was verified by sequencing. p.Gly23Val-MRAS missense mutations were engineered into pcDNA3.1- MRAS -C-HA vector using primers containing the missense mutations with the Quikchange II XL Site-Directed Mutagenesis Kit (Stratagene). .. Primary antibodies were used at the following dilutions: anti-phospho-ERK1/2 (1:1,000, Cell Signaling 9101) in 3% BSA, anti-ERK1/2 (1:1,000, Cell Signaling 9102) in 3% BSA, anti-HA tag (1:1,000, Santa Cruz Biotechnology SC-7392) in 3% BSA, and β-actin (1:5,000, Sigma-Aldrich A2228) in 5% nonfat dried milk.

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    GenScript bamhi 3 xhoi sites
    Bamhi 3 Xhoi Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi 3 xhoi sites/product/GenScript
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bamhi 3 xhoi sites - by Bioz Stars, 2020-07
    91/100 stars
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