Journal: Nature Communications

Article Title: Personalized bacteriophage therapy to treat pandrug-resistant spinal Pseudomonas aeruginosa infection

doi: 10.1038/s41467-022-31837-9

Figure Lengend Snippet: A EM micrographs of phage vB_PaeP_4029; B EM micrographs of phage vB_PaeP_4032; C EM micrographs of phage vB_PaeP_4034; D Phagogram (spot test) of phages vB_PaeP_4034 and vB_PaeP_4029 on production strain PAO1; E Phagogram (spot test) of phage vB_PaeP_4032 on production strain ATCC-15442-MINI; F Phagogram (spot test) of phages vB_PaeP_4034, vB_PaeP_4032, and vB_PaeP_4029 on the patient strain; G One of the two P. aeruginosa morphotypes cultured from a bone sample taken from the second-stage surgery procedure, that was close to the initial strain; H One of the two P. aeruginosa corresponds to a stable Small Colony Variant (SCV) morphotype; I Visualization of Plaque Forming Units (PFU) of the phage vB_PaeP_4029 on the patient’s strains isolated before phage therapy or during the second-stage surgery procedure; J Visualization of Plaque Forming Units (PFU) of the phage vB_PaeP_4032 on the patient’s strains isolated before phage therapy or during the second-stage surgery procedure; K Visualization of Plaque Forming Units (PFU) of the phage vB_PaeP_4034 on the patient’s strains isolated before phage therapy or during the second-stage surgery procedure.

Article Snippet: A EM micrographs of phage vB_PaeP_4029; B EM micrographs of phage vB_PaeP_4032; C EM micrographs of phage vB_PaeP_4034; D Phagogram (spot test) of phages vB_PaeP_4034 and vB_PaeP_4029 on production strain PAO1; E Phagogram (spot test) of phage vB_PaeP_4032 on production strain ATCC-15442-MINI; F Phagogram (spot test) of phages vB_PaeP_4034, vB_PaeP_4032, and vB_PaeP_4029 on the patient strain; G One of the two P. aeruginosa morphotypes cultured from a bone sample taken from the second-stage surgery procedure, that was close to the initial strain; H One of the two P. aeruginosa corresponds to a stable Small Colony Variant (SCV) morphotype; I Visualization of Plaque Forming Units (PFU) of the phage vB_PaeP_4029 on the patient’s strains isolated before phage therapy or during the second-stage surgery procedure; J Visualization of Plaque Forming Units (PFU) of the phage vB_PaeP_4032 on the patient’s strains isolated before phage therapy or during the second-stage surgery procedure; K Visualization of Plaque Forming Units (PFU) of the phage vB_PaeP_4034 on the patient’s strains isolated before phage therapy or during the second-stage surgery procedure.

Techniques: Spot Test, Cell Culture, Variant Assay, Isolation