bacterial expression vector pet 15b  (Millipore)


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    Structured Review

    Millipore bacterial expression vector pet 15b
    Bacterial Expression Vector Pet 15b, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial expression vector pet 15b/product/Millipore
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bacterial expression vector pet 15b - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Enzyme Architecture: The Role of a Flexible Loop in Activation of Glycerol-3-phosphate Dehydrogenase for Catalysis of Hydride Transfer
    Article Snippet: Paragraph title: Cloning and Site-Directed Mutagenesis of Human Liver Glycerol-3-phosphate Dehydrogenase ... The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis.

    Article Title: Human Glycerol 3-Phosphate Dehydrogenase: X-Ray Crystal Structures that Guide the Interpretation of Mutagenesis Studies.
    Article Snippet: Paragraph title: Cloning and Site-Directed Mutagenesis of Human Liver Glycerol Phosphate Dehydrogenase. ... The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis.

    Positron Emission Tomography:

    Article Title: The Activating Oxydianion Binding Domain for Enzyme-Catalyzed Proton Transfer, Hydride Transfer, and Decarboxylation: Specificity and Enzyme Architecture
    Article Snippet: .. The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for transformations of cells from E.coli strain Bl 21 (DE3). ..

    Article Title: Enzyme Architecture: The Role of a Flexible Loop in Activation of Glycerol-3-phosphate Dehydrogenase for Catalysis of Hydride Transfer
    Article Snippet: .. The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. Site-directed mutagenesis on pET-15b to introduce the mutations was carried out using the Quickchange II kit from Stratagene.

    Article Title: Human Glycerol 3-Phosphate Dehydrogenase: X-Ray Crystal Structures that Guide the Interpretation of Mutagenesis Studies.
    Article Snippet: .. The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. Site-directed mutagenesis on pET-15b to introduce the mutations was carried out using the Quikchange II kit from Stratagene.

    Mutagenesis:

    Article Title: The Activating Oxydianion Binding Domain for Enzyme-Catalyzed Proton Transfer, Hydride Transfer, and Decarboxylation: Specificity and Enzyme Architecture
    Article Snippet: Enzymes The C155S mutant of OMPDC from Saccharomyces cerevisiae (Sc OMPDC) was prepared according to a literature procedure and stored at −80 °C., The enzyme was defrosted and dialyzed at 4 °C against 10 mM MOPS (50% free base) at pH 7.1 containing 100 mM NaCl, unless noted otherwise. .. The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for transformations of cells from E.coli strain Bl 21 (DE3).

    Article Title: Enzyme Architecture: The Role of a Flexible Loop in Activation of Glycerol-3-phosphate Dehydrogenase for Catalysis of Hydride Transfer
    Article Snippet: .. The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. Site-directed mutagenesis on pET-15b to introduce the mutations was carried out using the Quickchange II kit from Stratagene.

    Article Title: Human Glycerol 3-Phosphate Dehydrogenase: X-Ray Crystal Structures that Guide the Interpretation of Mutagenesis Studies.
    Article Snippet: .. The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. Site-directed mutagenesis on pET-15b to introduce the mutations was carried out using the Quikchange II kit from Stratagene.

    Introduce:

    Article Title: Enzyme Architecture: The Role of a Flexible Loop in Activation of Glycerol-3-phosphate Dehydrogenase for Catalysis of Hydride Transfer
    Article Snippet: The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. Site-directed mutagenesis on pET-15b to introduce the mutations was carried out using the Quickchange II kit from Stratagene.

    Article Title: Human Glycerol 3-Phosphate Dehydrogenase: X-Ray Crystal Structures that Guide the Interpretation of Mutagenesis Studies.
    Article Snippet: The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. Site-directed mutagenesis on pET-15b to introduce the mutations was carried out using the Quikchange II kit from Stratagene.

    Purification:

    Article Title: The Activating Oxydianion Binding Domain for Enzyme-Catalyzed Proton Transfer, Hydride Transfer, and Decarboxylation: Specificity and Enzyme Architecture
    Article Snippet: Wildtype TIM from Saccharomyces cerevisiae (Sc TIM) was expressed from the TIM-deficient tpi A– λDE3 lysogenic strain of Escherichia coli , purified by a literature procedure, and stored at −80 °C in 25 mM Tris-HCl at pH 8.0 and I = 0.1 (NaCl) containing 20% glycerol. .. The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for transformations of cells from E.coli strain Bl 21 (DE3).

    Article Title: Enzyme Architecture: The Role of a Flexible Loop in Activation of Glycerol-3-phosphate Dehydrogenase for Catalysis of Hydride Transfer
    Article Snippet: The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. The primers used to introduce base changes encoding Q295A, Q295G, Q295S, and Q295N mutations differ from the sequence for the wild-type gene as follows: wild-type: 5′-GAAAGAGTTGCTGAATGGG CAG AAACTGCAGGGGCCCGAG-3′ Q295A: 5′-GAAAGAGTTGCTGAATGGG GCA AAACTGCAGGGGCCCGAG-3′ Q295G: 5′-GAAAGAGTTGCTGAATGGG GGA AAACTGCAGGGGCCCGAG-3′ Q295S: 5′-GAAAGAGTTGCTGAATGGG AGC AAACTGCAGGGGCCCGAG-3′ Q295N: 5′-GAAAGAGTTGCTGAATGGG AAC AAACTGCAGGGGCCCGAG-3′ The Q295 mutants were constructed individually starting with 20 ng of plasmid pET-15b containing the gene for wild-type hl GPDH that had been purified from Escherichia coli BL21 (DE3) cells.

    Article Title: Human Glycerol 3-Phosphate Dehydrogenase: X-Ray Crystal Structures that Guide the Interpretation of Mutagenesis Studies.
    Article Snippet: The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. The primers used to introduce base changes encoding for the K120A, D260G, R269A and Q295A mutants differ from the sequence for the wild type gene as follows: K120A Mutants: Wild type: 5’-CTGGCATATCTCTTATT AAG GGGGTAGACGAGGGC-3’ K120A: 5’-CTGGCATATCTCTTATT GCG GGGGTAGACGAGGGC-3’ D260G Mutants: Wild type: 5’-GAGAGCTGTGGTGTTGCT GAC CTGATCACTACCTGCTATG-3’ D260G: 5’-GAGAGCTGTGGTGTTGCT GGC CTGATCACTACCTGCTATG-3’ R269A Mutants: Wild type: 5´-CCTGCTATGGAGGG CGG AACCGGAAAGTGGCTGAGGCC-3´ R269A: 5´-CCTGCTATGGAGGG GCG AACCGGAAAGTGGCTGAGGCC-3´ Q295A Mutants: Wild type: 5′-GAAAGAGTTGCTGAATGGG CAG AAACTGCAGGGGCCCGAG-3′ Q295A: 5′-GAAAGAGTTGCTGAATGGG GCA AAACTGCAGGGGCCCGAG-3′ The K120 and D260 mutants were constructed individually, by published procedures, starting with 20 ng of plasmid pET-15b containing the gene for wild type hl GPDH that had been purified from Escherichia coli BL21 (DE3) cells.

    Sequencing:

    Article Title: Enzyme Architecture: The Role of a Flexible Loop in Activation of Glycerol-3-phosphate Dehydrogenase for Catalysis of Hydride Transfer
    Article Snippet: The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. The primers used to introduce base changes encoding Q295A, Q295G, Q295S, and Q295N mutations differ from the sequence for the wild-type gene as follows: wild-type: 5′-GAAAGAGTTGCTGAATGGG CAG AAACTGCAGGGGCCCGAG-3′ Q295A: 5′-GAAAGAGTTGCTGAATGGG GCA AAACTGCAGGGGCCCGAG-3′ Q295G: 5′-GAAAGAGTTGCTGAATGGG GGA AAACTGCAGGGGCCCGAG-3′ Q295S: 5′-GAAAGAGTTGCTGAATGGG AGC AAACTGCAGGGGCCCGAG-3′ Q295N: 5′-GAAAGAGTTGCTGAATGGG AAC AAACTGCAGGGGCCCGAG-3′ The Q295 mutants were constructed individually starting with 20 ng of plasmid pET-15b containing the gene for wild-type hl GPDH that had been purified from Escherichia coli BL21 (DE3) cells.

    Article Title: Human Glycerol 3-Phosphate Dehydrogenase: X-Ray Crystal Structures that Guide the Interpretation of Mutagenesis Studies.
    Article Snippet: The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. The primers used to introduce base changes encoding for the K120A, D260G, R269A and Q295A mutants differ from the sequence for the wild type gene as follows: K120A Mutants: Wild type: 5’-CTGGCATATCTCTTATT AAG GGGGTAGACGAGGGC-3’ K120A: 5’-CTGGCATATCTCTTATT GCG GGGGTAGACGAGGGC-3’ D260G Mutants: Wild type: 5’-GAGAGCTGTGGTGTTGCT GAC CTGATCACTACCTGCTATG-3’ D260G: 5’-GAGAGCTGTGGTGTTGCT GGC CTGATCACTACCTGCTATG-3’ R269A Mutants: Wild type: 5´-CCTGCTATGGAGGG CGG AACCGGAAAGTGGCTGAGGCC-3´ R269A: 5´-CCTGCTATGGAGGG GCG AACCGGAAAGTGGCTGAGGCC-3´ Q295A Mutants: Wild type: 5′-GAAAGAGTTGCTGAATGGG CAG AAACTGCAGGGGCCCGAG-3′ Q295A: 5′-GAAAGAGTTGCTGAATGGG GCA AAACTGCAGGGGCCCGAG-3′ The K120 and D260 mutants were constructed individually, by published procedures, starting with 20 ng of plasmid pET-15b containing the gene for wild type hl GPDH that had been purified from Escherichia coli BL21 (DE3) cells.

    Construct:

    Article Title: Enzyme Architecture: The Role of a Flexible Loop in Activation of Glycerol-3-phosphate Dehydrogenase for Catalysis of Hydride Transfer
    Article Snippet: The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. The primers used to introduce base changes encoding Q295A, Q295G, Q295S, and Q295N mutations differ from the sequence for the wild-type gene as follows: wild-type: 5′-GAAAGAGTTGCTGAATGGG CAG AAACTGCAGGGGCCCGAG-3′ Q295A: 5′-GAAAGAGTTGCTGAATGGG GCA AAACTGCAGGGGCCCGAG-3′ Q295G: 5′-GAAAGAGTTGCTGAATGGG GGA AAACTGCAGGGGCCCGAG-3′ Q295S: 5′-GAAAGAGTTGCTGAATGGG AGC AAACTGCAGGGGCCCGAG-3′ Q295N: 5′-GAAAGAGTTGCTGAATGGG AAC AAACTGCAGGGGCCCGAG-3′ The Q295 mutants were constructed individually starting with 20 ng of plasmid pET-15b containing the gene for wild-type hl GPDH that had been purified from Escherichia coli BL21 (DE3) cells.

    Article Title: Human Glycerol 3-Phosphate Dehydrogenase: X-Ray Crystal Structures that Guide the Interpretation of Mutagenesis Studies.
    Article Snippet: The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. The primers used to introduce base changes encoding for the K120A, D260G, R269A and Q295A mutants differ from the sequence for the wild type gene as follows: K120A Mutants: Wild type: 5’-CTGGCATATCTCTTATT AAG GGGGTAGACGAGGGC-3’ K120A: 5’-CTGGCATATCTCTTATT GCG GGGGTAGACGAGGGC-3’ D260G Mutants: Wild type: 5’-GAGAGCTGTGGTGTTGCT GAC CTGATCACTACCTGCTATG-3’ D260G: 5’-GAGAGCTGTGGTGTTGCT GGC CTGATCACTACCTGCTATG-3’ R269A Mutants: Wild type: 5´-CCTGCTATGGAGGG CGG AACCGGAAAGTGGCTGAGGCC-3´ R269A: 5´-CCTGCTATGGAGGG GCG AACCGGAAAGTGGCTGAGGCC-3´ Q295A Mutants: Wild type: 5′-GAAAGAGTTGCTGAATGGG CAG AAACTGCAGGGGCCCGAG-3′ Q295A: 5′-GAAAGAGTTGCTGAATGGG GCA AAACTGCAGGGGCCCGAG-3′ The K120 and D260 mutants were constructed individually, by published procedures, starting with 20 ng of plasmid pET-15b containing the gene for wild type hl GPDH that had been purified from Escherichia coli BL21 (DE3) cells.

    Expressing:

    Article Title: The Activating Oxydianion Binding Domain for Enzyme-Catalyzed Proton Transfer, Hydride Transfer, and Decarboxylation: Specificity and Enzyme Architecture
    Article Snippet: .. The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for transformations of cells from E.coli strain Bl 21 (DE3). ..

    Article Title: Enzyme Architecture: The Role of a Flexible Loop in Activation of Glycerol-3-phosphate Dehydrogenase for Catalysis of Hydride Transfer
    Article Snippet: .. The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. Site-directed mutagenesis on pET-15b to introduce the mutations was carried out using the Quickchange II kit from Stratagene.

    Article Title: Human Glycerol 3-Phosphate Dehydrogenase: X-Ray Crystal Structures that Guide the Interpretation of Mutagenesis Studies.
    Article Snippet: .. The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. Site-directed mutagenesis on pET-15b to introduce the mutations was carried out using the Quikchange II kit from Stratagene.

    Polymerase Chain Reaction:

    Article Title: Enzyme Architecture: The Role of a Flexible Loop in Activation of Glycerol-3-phosphate Dehydrogenase for Catalysis of Hydride Transfer
    Article Snippet: The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. This plasmid was added to a PCR mixture containing 5 μL of 10X Pfu Ultra Buffer, 125 ng each of the forward and reverse mutagenesis primers, 1 μL of 10 mM dNTP mixture, 2.5 units of Pfu Ultra HF DNA polymerase, and water to give a final volume of 50 μL.

    Over Expression:

    Article Title: The Activating Oxydianion Binding Domain for Enzyme-Catalyzed Proton Transfer, Hydride Transfer, and Decarboxylation: Specificity and Enzyme Architecture
    Article Snippet: The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for transformations of cells from E.coli strain Bl 21 (DE3). .. After 12 h of overexpression, the cells were harvested and stored in 20 mL of 25 mM Tris-HCl buffer that contains 0.5 M NaCl at pH 7.9.

    Plasmid Preparation:

    Article Title: The Activating Oxydianion Binding Domain for Enzyme-Catalyzed Proton Transfer, Hydride Transfer, and Decarboxylation: Specificity and Enzyme Architecture
    Article Snippet: .. The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for transformations of cells from E.coli strain Bl 21 (DE3). ..

    Article Title: Enzyme Architecture: The Role of a Flexible Loop in Activation of Glycerol-3-phosphate Dehydrogenase for Catalysis of Hydride Transfer
    Article Snippet: .. The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. Site-directed mutagenesis on pET-15b to introduce the mutations was carried out using the Quickchange II kit from Stratagene.

    Article Title: Human Glycerol 3-Phosphate Dehydrogenase: X-Ray Crystal Structures that Guide the Interpretation of Mutagenesis Studies.
    Article Snippet: .. The insert gene was subcloned into a bacterial expression vector pET-15b from Novagen and used for mutagenesis. .. Site-directed mutagenesis on pET-15b to introduce the mutations was carried out using the Quikchange II kit from Stratagene.

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  • Team
  • Advisors
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  • 98
    Millipore expression vector pet 15b
    Expression Vector Pet 15b, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression vector pet 15b/product/Millipore
    Average 98 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    expression vector pet 15b - by Bioz Stars, 2020-04
    98/100 stars
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