bacterial bl21 de3  (Millipore)


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  • 95

    Structured Review

    Millipore bacterial bl21 de3
    Bacterial Bl21 De3, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial bl21 de3/product/Millipore
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bacterial bl21 de3 - by Bioz Stars, 2020-04
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Characterization of a novel AICARFT inhibitor which potently elevates ZMP and has anti-tumor activity in murine models
    Article Snippet: Paragraph title: Cloning and Enzyme Purification ... Bacterial BL21 (DE3) (Novagen) was used as an expression host, and protein purification is described in supplemental material.

    Synthesized:

    Article Title: Characterization of a novel AICARFT inhibitor which potently elevates ZMP and has anti-tumor activity in murine models
    Article Snippet: The human MTHFD2 (accession number: ) and MTHFD2L (accession number: NM_001144978.1) cDNAs were synthesized at GenScript. .. Bacterial BL21 (DE3) (Novagen) was used as an expression host, and protein purification is described in supplemental material.

    Mutagenesis:

    Article Title: Characterization of a novel AICARFT inhibitor which potently elevates ZMP and has anti-tumor activity in murine models
    Article Snippet: Human MTHFD1 (accession number: NM_005956.3) cDNA was purchased from Openbiosystem Co. (Cat# MHS1011-169900, Clone ID: 3508998, accession: ), which contains two mutations and was fixed by PCR-based mutagenesis. .. Bacterial BL21 (DE3) (Novagen) was used as an expression host, and protein purification is described in supplemental material.

    Purification:

    Article Title: Characterization of a novel AICARFT inhibitor which potently elevates ZMP and has anti-tumor activity in murine models
    Article Snippet: Paragraph title: Cloning and Enzyme Purification ... Bacterial BL21 (DE3) (Novagen) was used as an expression host, and protein purification is described in supplemental material.

    Protein Purification:

    Article Title: Characterization of a novel AICARFT inhibitor which potently elevates ZMP and has anti-tumor activity in murine models
    Article Snippet: .. Bacterial BL21 (DE3) (Novagen) was used as an expression host, and protein purification is described in supplemental material. .. Preparation of the 10 formyl-tetrahydrofolate substrate used in the ATIC enzyme assay is a two-step process , starting from folinic acid (Sigma CAS 1492-18-8).

    Expressing:

    Article Title: Characterization of a novel AICARFT inhibitor which potently elevates ZMP and has anti-tumor activity in murine models
    Article Snippet: .. Bacterial BL21 (DE3) (Novagen) was used as an expression host, and protein purification is described in supplemental material. .. Preparation of the 10 formyl-tetrahydrofolate substrate used in the ATIC enzyme assay is a two-step process , starting from folinic acid (Sigma CAS 1492-18-8).

    Polymerase Chain Reaction:

    Article Title: Characterization of a novel AICARFT inhibitor which potently elevates ZMP and has anti-tumor activity in murine models
    Article Snippet: Human MTHFD1 (accession number: NM_005956.3) cDNA was purchased from Openbiosystem Co. (Cat# MHS1011-169900, Clone ID: 3508998, accession: ), which contains two mutations and was fixed by PCR-based mutagenesis. .. Bacterial BL21 (DE3) (Novagen) was used as an expression host, and protein purification is described in supplemental material.

    Plasmid Preparation:

    Article Title: Characterization of a novel AICARFT inhibitor which potently elevates ZMP and has anti-tumor activity in murine models
    Article Snippet: The nucleotide sequences encoding full-length human AICARFT, TS, SHMT, MTHFD1, and partial sequences MTHFD2 (32–350) and MTHFD2L (46–347) were inserted into pET21d (Novagen) vector with an N-terminal HIS tag. .. Bacterial BL21 (DE3) (Novagen) was used as an expression host, and protein purification is described in supplemental material.

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  • 90
    Millipore strain bl21
    Strain Bl21, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore bacteria bl21
    Bacteria Bl21, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 2 article reviews
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    Millipore bl21 de3 bacteria
    Bl21 De3 Bacteria, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 bacteria/product/Millipore
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 bacteria - by Bioz Stars, 2020-04
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