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murine probdnf  (Alomone Labs)


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    Alomone Labs murine probdnf
    Specificity of neurite growth-promoting effects of proNGF, <t>proBDNF</t> and proNT3. ( A , B ) Neurite length and branching of E17 to P5 trigeminal neurons cultured for 24 hours without factors or with 10 <t>ng/ml</t> <t>cleavage-resistant</t> murine proNGF or 10 ng/ml mNGF. ( C , D ) Neurite length and branching of P5 trigeminal neurons cultured with a range of concentrations of mNGF and cleavage-resistant murine proNGF. ( E-G ) YFP-transfected SCG neurons (green) and pDsRed-transfected trigeminal neurons (red) co-cultured for 24 hours either without factors (E), or with 10 ng/ml cleavage-resistant murine proNGF (F) or 10 ng/ml mNGF (G). Scale bars: 100 μm. ( H ) Sholl plot of P3 trigeminal neurons incubated with mNGF, cleavage-resistant murine proNGF or cleavage-resistant human proNGF. ( I , J ) Neurite length and branching of P3 SCG, trigeminal and nodose neurons cultured for 24 hours with 10 ng/ml cleavage-resistant murine proNGF, 10 ng/ml cleavage-resistant murine proBDNF or 10 ng/ml native human proNT3. All cultures were supplemented with 25 μM Boc-D-FMK and the cultures with native proNT3 additionally received 1 μM batimastat. The mean±s.e.m. for at least three individual datasets are shown.
    Murine Probdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b-457/pmc03640218-37-18-34?v=Alomone+Labs
    Average 90 stars, based on 1 article reviews
    murine probdnf - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "ProNGF promotes neurite growth from a subset of NGF-dependent neurons by a p75 NTR -dependent mechanism"

    Article Title: ProNGF promotes neurite growth from a subset of NGF-dependent neurons by a p75 NTR -dependent mechanism

    Journal: Development (Cambridge, England)

    doi: 10.1242/dev.085266

    Specificity of neurite growth-promoting effects of proNGF, proBDNF and proNT3. ( A , B ) Neurite length and branching of E17 to P5 trigeminal neurons cultured for 24 hours without factors or with 10 ng/ml cleavage-resistant murine proNGF or 10 ng/ml mNGF. ( C , D ) Neurite length and branching of P5 trigeminal neurons cultured with a range of concentrations of mNGF and cleavage-resistant murine proNGF. ( E-G ) YFP-transfected SCG neurons (green) and pDsRed-transfected trigeminal neurons (red) co-cultured for 24 hours either without factors (E), or with 10 ng/ml cleavage-resistant murine proNGF (F) or 10 ng/ml mNGF (G). Scale bars: 100 μm. ( H ) Sholl plot of P3 trigeminal neurons incubated with mNGF, cleavage-resistant murine proNGF or cleavage-resistant human proNGF. ( I , J ) Neurite length and branching of P3 SCG, trigeminal and nodose neurons cultured for 24 hours with 10 ng/ml cleavage-resistant murine proNGF, 10 ng/ml cleavage-resistant murine proBDNF or 10 ng/ml native human proNT3. All cultures were supplemented with 25 μM Boc-D-FMK and the cultures with native proNT3 additionally received 1 μM batimastat. The mean±s.e.m. for at least three individual datasets are shown.
    Figure Legend Snippet: Specificity of neurite growth-promoting effects of proNGF, proBDNF and proNT3. ( A , B ) Neurite length and branching of E17 to P5 trigeminal neurons cultured for 24 hours without factors or with 10 ng/ml cleavage-resistant murine proNGF or 10 ng/ml mNGF. ( C , D ) Neurite length and branching of P5 trigeminal neurons cultured with a range of concentrations of mNGF and cleavage-resistant murine proNGF. ( E-G ) YFP-transfected SCG neurons (green) and pDsRed-transfected trigeminal neurons (red) co-cultured for 24 hours either without factors (E), or with 10 ng/ml cleavage-resistant murine proNGF (F) or 10 ng/ml mNGF (G). Scale bars: 100 μm. ( H ) Sholl plot of P3 trigeminal neurons incubated with mNGF, cleavage-resistant murine proNGF or cleavage-resistant human proNGF. ( I , J ) Neurite length and branching of P3 SCG, trigeminal and nodose neurons cultured for 24 hours with 10 ng/ml cleavage-resistant murine proNGF, 10 ng/ml cleavage-resistant murine proBDNF or 10 ng/ml native human proNT3. All cultures were supplemented with 25 μM Boc-D-FMK and the cultures with native proNT3 additionally received 1 μM batimastat. The mean±s.e.m. for at least three individual datasets are shown.

    Techniques Used: Cell Culture, Transfection, Incubation



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    Specificity of neurite growth-promoting effects of proNGF, proBDNF and proNT3. ( A , B ) Neurite length and branching of E17 to P5 trigeminal neurons cultured for 24 hours without factors or with 10 ng/ml cleavage-resistant murine proNGF or 10 ng/ml mNGF. ( C , D ) Neurite length and branching of P5 trigeminal neurons cultured with a range of concentrations of mNGF and cleavage-resistant murine proNGF. ( E-G ) YFP-transfected SCG neurons (green) and pDsRed-transfected trigeminal neurons (red) co-cultured for 24 hours either without factors (E), or with 10 ng/ml cleavage-resistant murine proNGF (F) or 10 ng/ml mNGF (G). Scale bars: 100 μm. ( H ) Sholl plot of P3 trigeminal neurons incubated with mNGF, cleavage-resistant murine proNGF or cleavage-resistant human proNGF. ( I , J ) Neurite length and branching of P3 SCG, trigeminal and nodose neurons cultured for 24 hours with 10 ng/ml cleavage-resistant murine proNGF, 10 ng/ml cleavage-resistant murine proBDNF or 10 ng/ml native human proNT3. All cultures were supplemented with 25 μM Boc-D-FMK and the cultures with native proNT3 additionally received 1 μM batimastat. The mean±s.e.m. for at least three individual datasets are shown.

    Journal: Development (Cambridge, England)

    Article Title: ProNGF promotes neurite growth from a subset of NGF-dependent neurons by a p75 NTR -dependent mechanism

    doi: 10.1242/dev.085266

    Figure Lengend Snippet: Specificity of neurite growth-promoting effects of proNGF, proBDNF and proNT3. ( A , B ) Neurite length and branching of E17 to P5 trigeminal neurons cultured for 24 hours without factors or with 10 ng/ml cleavage-resistant murine proNGF or 10 ng/ml mNGF. ( C , D ) Neurite length and branching of P5 trigeminal neurons cultured with a range of concentrations of mNGF and cleavage-resistant murine proNGF. ( E-G ) YFP-transfected SCG neurons (green) and pDsRed-transfected trigeminal neurons (red) co-cultured for 24 hours either without factors (E), or with 10 ng/ml cleavage-resistant murine proNGF (F) or 10 ng/ml mNGF (G). Scale bars: 100 μm. ( H ) Sholl plot of P3 trigeminal neurons incubated with mNGF, cleavage-resistant murine proNGF or cleavage-resistant human proNGF. ( I , J ) Neurite length and branching of P3 SCG, trigeminal and nodose neurons cultured for 24 hours with 10 ng/ml cleavage-resistant murine proNGF, 10 ng/ml cleavage-resistant murine proBDNF or 10 ng/ml native human proNT3. All cultures were supplemented with 25 μM Boc-D-FMK and the cultures with native proNT3 additionally received 1 μM batimastat. The mean±s.e.m. for at least three individual datasets are shown.

    Article Snippet: Native murine proNGF, cleavage-resistant murine proNGF that has an R-to-G substitution at amino acid position 104 and cleavage-resistant murine proBDNF that has RR-to-AG substitutions at amino acid positions 112 and 113 were obtained from Alomone (Jerusalem, Israel).

    Techniques: Cell Culture, Transfection, Incubation