recombinant human probdnf protein  (Alomone Labs)

Journal: iScience

Article Title: Cytokine profile of anti-spike CD4 + T cells predicts humoral and CD8 + T cell responses after anti-SARS-CoV-2 mRNA vaccination

doi: 10.1016/j.isci.2024.110441

Figure Lengend Snippet: Value of the CD4 + T cells derived cytokine signature in predicting CD8 + T cell response in mice Mice ( n = 4) were immunized by the nasal route with ovalbumin (OVA; 100 mg), either alone or combined with the adjuvant C-di-GMP (10 mg), on D0 and D14. On D21, BAL was recovered and the frequency of CD8 + T cells was quantified using an OVA 257-264 H-2K b dextramer. After perfusion, lungs were harvested, and purified CD4 + T cells (10 5 cells) were plated in 96-well plates with splenocytes derived from naive mice that were either sensitized or not with the long OVA peptide (TEWTSSNVMEERKIKV [OVA 265–280] ). After 36 h, supernatants were collected and tested for the presence of IL-9, IL-2, TNFα, and IP-10. (A) Induction of anti-OVA 257-264 CD8 + T cells in mice vaccinated with OVA alone or in combination with the adjuvant C-di-GMP. (B) Cytokine concentrations in CD4 + T cells supernatants derived from lung, sensitized with the long OVA peptide. The Mann Whitney statistical test was used for the analysis; Data are represented as mean ± SEM. ∗: p ≤ 0.05; ∗∗: p ≤ 0.01; ∗∗∗: p ≤ 0.001.

Article Snippet: Cells were stained with K b -OVA 257-264 dextramer (Immudex) at room temperature for 20 min. After washing with PBS, cells were incubated for 20 min at 4°C with LIVE/DEAD Fixable Aqua Dead Cell Stain (Invitrogen), CD3 PercpCy5.5 (eBioscience), and CD8 BUV496 (Becton Dickinson (BD) Biosciences).

Techniques: Derivative Assay, Adjuvant, Purification, MANN-WHITNEY

Journal: iScience

Article Title: Cytokine profile of anti-spike CD4 + T cells predicts humoral and CD8 + T cell responses after anti-SARS-CoV-2 mRNA vaccination

doi: 10.1016/j.isci.2024.110441

Figure Lengend Snippet:

Article Snippet: Cells were stained with K b -OVA 257-264 dextramer (Immudex) at room temperature for 20 min. After washing with PBS, cells were incubated for 20 min at 4°C with LIVE/DEAD Fixable Aqua Dead Cell Stain (Invitrogen), CD3 PercpCy5.5 (eBioscience), and CD8 BUV496 (Becton Dickinson (BD) Biosciences).

Techniques: Blocking Assay, Recombinant, Staining, Enzyme-linked Immunospot, Multiplex Assay, Selection, Software, Gentle