biotinylated bdnf (Alomone Labs)
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biotinylated bdnf
Biotinylated Bdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b-250-b/bio_rxiv__64898__2026__02__27__707919-57-9-18?v=Alomone+Labs
Average 94 stars, based on 21 article reviews
Biotinylated Bdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b-250-b/bio_rxiv__64898__2026__02__27__707919-57-9-18?v=Alomone+Labs
Average 94 stars, based on 21 article reviews
biotinylated bdnf - by Bioz Stars,
2026-07
94/100 stars
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1) Product Images from "Fingolimod acutely facilitates the activation of TRKB"
Article Title: Fingolimod acutely facilitates the activation of TRKB
Journal: bioRxiv
doi: 10.64898/2026.02.27.707919
Figure Legend Snippet: TRKB signaling in vitro , related to . (A) Quantification of TRKB mRNA levels by qPCR in N2A cells non-transfected and transfected with TRKB.wt plasmid. (B) 30min BDNF treatment (10ng/ml) increase TRKB homodimerization by PCA in N2A cells. Unpaired t-test, t=8,351, df=30, p<0.0001, n=16. (C) BDNF (10ng/ml) increased TRKB phosphorylation at tyrosine 816 in cortical neurons in ELISA. Unpaired t-test, t=3,433, df=14, p=0.004, n=8. (D) BDNF (10ng/ml) increased interaction in cortical neurons between TRKB and its signaling partner PLCgamma1 in ELISA. Unpaired t-test, t=2,646, df=13, p=0.02, n=7/8. (E) BDNF (10ng/ml) promotes interaction between TRKB and activated Src family kinase in cortical neurons measured by ELISA. Unpaired t-test, t=2,512, df=12, p=0.027, n=6/8. Data is expressed as mean+SEM.
Techniques Used: In Vitro, Transfection, Plasmid Preparation, Phospho-proteomics, Enzyme-linked Immunosorbent Assay
Figure Legend Snippet: FNG induces TRKB signaling in vitro . (A) Acute administration of FNG (0.1, 1, 10 μM, 30 min) on N2A cells induces TRKB homodimerization (n=8/group) in PCA. Statistical analysis performed by One-way ANOVA and Tukey’s post hoc test, F (3, 76) = 13,05 p(0.1 μM)=0.0013, p(1 μM)=0.007, p(10 μM)=0.0011. (B) Acute administration of FNG and the effect on TRKB phosphorylation at tyrosine 816 measured by ELISA in primary cultured cortical cells from rat embryos (n=12/group). One-way ANOVA and Šidák’s post hoc test, F (3, 44) = 18,11, p<0.0001. (C) Acute administration of 10 μM FNG on TRKB:PLCg1 interaction measured by ELISA (n=5/group). Unpaired t-test, t=3,734, df=8, p=0.0053. (D) Detection of plasma membrane localized TRKB following acute FNG treatment (n=18/group). Kruskal-Wallis and Dunn’s post hoc test, KW= 17,81, p=0.0011. (E) Effect of the BDNF-blocking peptide TRKB_FC on the effects of acute FNG treatment in TRKB-PLCg1 interaction measured by ELISA (n=18/group). Two-way ANOVA and Tukey’s post hoc test, F (1, 100) = 15,93 p=0.0001 for the treatment effect. Data is expressed as mean+SEM.
Techniques Used: In Vitro, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Cell Culture, Clinical Proteomics, Membrane, Blocking Assay
Figure Legend Snippet: TRKB activation by FNG is an early event in FNG action and requires BDNF. (A) Acute administration of FNG and the activation of Src family kinase measured by ELISA on primary rat cortical neurons (n=5/group). One way ANOVA and Fisher’s LSD, F (3, 16) = 40,13, p(1 μM, 10 μM)<0.0001. (B) Effect of acute FNG treatments on TKRB dimerization in N2A cells in presence of kinase inhibitors PP1 and k252a (n=12/group). One way ANOVA and Tuckey’s post hoc test, F (5, 66) = 19,72, p<0.0001. (C) Ligand binding assay for TRKB and biotinylated BDNF in presence of increasing dose of FNG (n= 6/group). (D) Acute administration of FNG does not induce dimerization of the TRKB mutant Y433F (n=12/group). Two-way ANOVA and Šidák’s post hoc test, F (2, 55) = 6,216, p(Y433F +/- )=0.0032, p(Y433F)=0.0002. Data is expressed as mean+SEM. (E) Effect of increasing concentration of FNG on the binding of biotinylated fluoxetine on TRKB (n=6/group).
Techniques Used: Activation Assay, Enzyme-linked Immunosorbent Assay, Ligand Binding Assay, Mutagenesis, Concentration Assay, Binding Assay
Figure Legend Snippet: The effect of FNG administration on contextual fear conditioning. (A) Combined effects of pravastatin (10mg/kg for 14 days in drinking water) and fingolimod (1mg/kg, 2h prior) on the ability of female mice to discriminate between context A and context B, expressed as discrimination index. Pravastatin treatment impairs the discrimination ability of the two contexts, Three-way ANOVA F(1, 35) = 30,66, p<0.0001. (B) 10 min foot shock conditioning session for BDNF.wt and BDNF.het mice. FNG treatment (1 mg/kg, 2h prior) did not affect the freezing behaviour. Two way ANOVA, F(1, 21) = 9,068, p=0,9925, while BDNF.hets generally freeze more compared to control, Two way ANOVA F(1, 21)=12.78, p=0.0018. (C) FNG treatment (1 mg/kg, 2h prior, blue bars) had no effect on BDNF.wt animals exposed for 5 min to a novel environment but could rescue the generalized freezing of the BDNF.het animals. F(1, 21) = 6,279, p(interaction)=0,0205, p(controls)=0.0001, p(BDNF.het)=0.004283 by two-way ANOVA and uncorrected Fisher’s LSD multiple comparisons (D) FNG treatment did not have any effect on the freezing behavior of BDNF.wt and BDNF.het mice placed back in the conditioning environment without foot shocks. Two way ANOVA, F (1, 21) = 0,05193, p=0,8219. Similarly as the conditioning, BDNF.het freeze more compared to wild-type littermates F(1, 21)=8.636, p= p=0.0078. In a cohort of animals pre-treated with pravastatin (10mg/kg for 14 days in drinking water) (Panels E,F,G) FNG (1mg/kg, 2h prior) did not affect the conditioning. BDNF.het mice freeze significantly more compared to control animals (E), Two way ANOVA F(1,16)=11.8, p=0.0034. (F) Similarly as for the condition, BDNF.het mice freeze more than the wt littermates, Two way ANOVA, F(1,16)=24.49; p=0.0001. FNG treatment (1mg/kg, 2h prior) could not rescue the freezing behaviour in BDNF.het mice and had no significant effects on BDNF.wt animals. (G) Animals placed back in the conditioning environment without foot shock did not show any behavioral effect after FNG treatment (1mg/kg, 2h prior). As in the previous conditions, BDNF.het mice generally freeze more than the wt, Two way ANOVA, F(1,16)=9.374, p=0.0075. Data expressed as mean+SEM, n=5/7 per group.
Techniques Used: Control