Journal: Applied and Environmental Microbiology
Article Title: Genome-Wide Assessment of Stress-Associated Genes in Bifidobacteria
doi: 10.1128/aem.02251-21
Figure Lengend Snippet: Identification of HemW-specific motifs in HemN homologs of B. animalis BB-12, B. longum NCC2705, and B. asteroides PRL2011. To assess the presence of HemW-specific motifs in the protein sequences of HemN homologs from B. animalis subsp. lactis BB-12 ( WP_004217870.1 ), B. longum subsp. longum NCC2705 ( WP_008783712.1 ), and B. asteroides PRL2011 ( WP_033511215.1 ), their protein sequences were aligned with HemW of L. lactis ( WP_003132086.1 ). The alignment was generated using CLC Genomics Workbench 20.0, default settings, alignment mode set to very accurate. Blue boxes are HemW-specific conserved amino acid residues that distinguish the protein from HemN, identified in L. lactis : H 134 , H 184 xxxYxLxxE, Y 234 ExS, and especially H 248 NxxYW .
Article Snippet: Strains lacking homologs were strains of the B. asteroides group, B. subtile , B. eulemuris DSM 100216 (only representative of B. eulemuris species), B. longum BORI and CCUG 30698, and B. animalis subsp. lactis ATCC 27673, which has already been described as having a genetic content distinct from that of other strains of the species ( ).
Techniques: Generated