b cenocepacia  (ATCC)

Journal: Respiratory Research

Article Title: Whole genome comparisons reveal gut-to-lung translocation of Escherichia coli and Burkholderia cenocepacia in two cases of ventilator-associated pneumonia in ICU patients

doi: 10.1186/s12931-025-03204-x

Figure Lengend Snippet: Characteristics of Ec1 and Bc1 strains isolated from ETA samples of two VAP patients. ( A ) MIC values for the Ec1 and Bc1 strains against various antibiotics are represented by solid circles. Aminoglycosides: kanamycin (KAN), amikacin (AMK), gentamicin (GEN); β_lactams: ampicillin (AMP), imipenem (IPM), cefazolin (CZO), ceftazidime (CAZ); Polypeptides: polymyxin B (poly B), polymyxin E (poly E); Chloramphenicols: chloromycetin (CM); Fluoroquinolones: levofloxacin (LEVO); Phosphonate: fosfomycin (FOS); Macrolides: erythrocin (ERY), azithromycin (AZM); quinolone: moxifloxacin (MXF); Tetracyclines: tetracycline (TET). ( B ) ARG profiles of Ec1 and Bc1 strains and their resistance to the antibiotic classes (orange present, azure absent). ( C ) Binary heatmap showing the presence (orange) or absence (azure) of virulence genes in Ec1 and Bc1 strains. ( D ) Survival curves of G. mellonella larvae infected with K12, Ec1, Bc and Bc1 strains for 96 h. The blue, green, orange, and purple curves represent the survival rates of larvae infected with K12, Ec1, Bc and Bc1, respectively. ( E ) Growth curves of K12 (blue), Ec1 (green), Bc (orange), and Bc1 (purple) strains, with eight replicates per strain ( n = 8). ( F ) Growth rate differences among K12, Ec1, Bc and Bc1 strains, calculated using the R package ( Grofit ) from the growth curve data. ( G ) Box plot illustrating the lag time (h) during growth for K12, Ec1, Bc, and Bc1 strains. ( H ) Box plot illustrating the maximum optical density (OD600) reached during growth for K12, Ec1, Bc, and Bc1 strains. ( I ) Box plot displaying biofilm formation (OD570) of K12, Ec1, Bc and Bc1 strains, with eight replicates per strain ( n = 8). Statistics by the unpaired two-tailed Student’s t test. Values shown are the mean ± SD. *** p < 0.001. ns : no significance. K12: standard E. coli K12 MG1655, Ec1: translocated E. coli , Bc: standard B. cenocepacia , Bc1: translocated B. cenocepacia

Article Snippet: G. mellonella killing assays were performed using K12 (standard E. coli K12 MG 1655), Ec1, Bc (standard B. cenocepacia ATCC BAA-245), and Bc1 strains.

Techniques: Isolation, Infection, Two Tailed Test

Journal: Respiratory Research

Article Title: Whole genome comparisons reveal gut-to-lung translocation of Escherichia coli and Burkholderia cenocepacia in two cases of ventilator-associated pneumonia in ICU patients

doi: 10.1186/s12931-025-03204-x

Figure Lengend Snippet: ANI analysis of pulmonary isolate genomes and metagenome-derived genomes. The upper layer represents the assembled draft genomes, while the lower layer displays the reference genomes of the isolated strains. ( A ) The ANI value between the Ec1 genome and its corresponding assembled draft genome obtained utilizing reference genome-based methods is 99.96%. ( B ) The ANI value between the Bc1 genome and its corresponding assembled draft genome obtained utilizing reference genome-based methods is 99.41%. ( C ) The ANI value between the Ec1 genome and its corresponding assembled draft genome based on de novo assembly and binning is 99.92%. ( D ) The ANI value between the Bc1 genome and its corresponding assembled draft genome based on de novo assembly and binning is 99.30%. Ec1: translocated E . coli , Bc1: translocated B . cenocepacia

Article Snippet: G. mellonella killing assays were performed using K12 (standard E. coli K12 MG 1655), Ec1, Bc (standard B. cenocepacia ATCC BAA-245), and Bc1 strains.

Techniques: Derivative Assay, Isolation