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b algerae  (ATCC)


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    ATCC b algerae
    Electron microscopic overview of infected human muscle biopsy tissue containing developmental stages of the microsporidial parasite, Brachiola <t>algerae</t> <t>(Hba).</t> 2. Early proliferative stages of the parasite in direct contact with the host muscle-cell cytoplasm (H). Note the presence of vesiculotubular appendages (arrow) attached to the parasite thickened plasmalemma, forming many dense bodies (arrowhead) in the host cytoplasm surrounding the parasite cells and abutting the host myofilaments (M). Bar = 1 μm. 3. Diplokaryotic proliferative parasite cells and spores (S). Note the attachment of the vesiculotubular appendages (arrows) to the proliferative cells but not to the spores. Dense appendage material is present in the space between parasite cells (arrowheads). Bar = 1 μm. 4. Low-power electron micrograph of clustered parasites in host cell cytoplasm (H). Host nuclei (N) and cytoplasm in direct contact with parasite cells and dense vesiculotubular appendage material (arrowheads) scattered throughout host cytoplasm. Note the lack of myofilaments between parasite cells. Bar = 1 μm. 5. All parasite stages are present and intermixed, an indication of asynchronous development. Cytokinesis and karyokinesis are linked, resulting in individual organisms containing one diplokaryon (D) or two with indications of cell division (*). Bar = 1 μm. 6. Proliferative cell containing a diplokaryon with condensed chromatin (arrows) and a spindle plaque (PL), indicating karyokinetic activity. Bar = 1 μm. 7. Proliferative cell containing a diplokaryon with condensed chromatin (arrows), extensive ER, and attached dense vesiculotubular appendages (arrowhead). Bar = 0.5 μm. 8–10. Sporonts. Note the change in appearance of the appendages (arrowhead) to clumps of dense material detaching from the plasmalemma. 8. Early sporont. Note the relatively lower cytoplasmic density when compared to the other two. Bar = 0.5 μm. 9. Note the presence of condensing chromatin in the sporont nucleus; it indicates the disporous nature of this parasite. Bar = 0.5 μm. 10. Note the abundance of dense vesiculotubular appendage material (arrowhead) in the process of being sloughed off the plasmalemma as it becomes more homogeneously dense. Bar = 0.5 μm. 11. Four sporoblasts with homogeneously thickened plasmalemma (after last cell division) and lacking attached appendages visible in the surrounding cytoplasm (arrowheads). Note the presence of the forming polar filament (PF). Bar = 0.5 μm. 12–13. Typical spores of B. algerae with 9–11 polar filament coils. 12. Early activated spore. Note the translocation of some polar filament coils. Bar = 0.5 μm. 13. Mature resting spore. Bar = 0.5 μm.
    B Algerae, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "An Analysis of the Microsporidian Genus Brachiola , with Comparisons of Human and Insect Isolates of Brachiola algerae"

    Article Title: An Analysis of the Microsporidian Genus Brachiola , with Comparisons of Human and Insect Isolates of Brachiola algerae

    Journal: The Journal of eukaryotic microbiology

    doi:

    Electron microscopic overview of infected human muscle biopsy tissue containing developmental stages of the microsporidial parasite, Brachiola algerae (Hba). 2. Early proliferative stages of the parasite in direct contact with the host muscle-cell cytoplasm (H). Note the presence of vesiculotubular appendages (arrow) attached to the parasite thickened plasmalemma, forming many dense bodies (arrowhead) in the host cytoplasm surrounding the parasite cells and abutting the host myofilaments (M). Bar = 1 μm. 3. Diplokaryotic proliferative parasite cells and spores (S). Note the attachment of the vesiculotubular appendages (arrows) to the proliferative cells but not to the spores. Dense appendage material is present in the space between parasite cells (arrowheads). Bar = 1 μm. 4. Low-power electron micrograph of clustered parasites in host cell cytoplasm (H). Host nuclei (N) and cytoplasm in direct contact with parasite cells and dense vesiculotubular appendage material (arrowheads) scattered throughout host cytoplasm. Note the lack of myofilaments between parasite cells. Bar = 1 μm. 5. All parasite stages are present and intermixed, an indication of asynchronous development. Cytokinesis and karyokinesis are linked, resulting in individual organisms containing one diplokaryon (D) or two with indications of cell division (*). Bar = 1 μm. 6. Proliferative cell containing a diplokaryon with condensed chromatin (arrows) and a spindle plaque (PL), indicating karyokinetic activity. Bar = 1 μm. 7. Proliferative cell containing a diplokaryon with condensed chromatin (arrows), extensive ER, and attached dense vesiculotubular appendages (arrowhead). Bar = 0.5 μm. 8–10. Sporonts. Note the change in appearance of the appendages (arrowhead) to clumps of dense material detaching from the plasmalemma. 8. Early sporont. Note the relatively lower cytoplasmic density when compared to the other two. Bar = 0.5 μm. 9. Note the presence of condensing chromatin in the sporont nucleus; it indicates the disporous nature of this parasite. Bar = 0.5 μm. 10. Note the abundance of dense vesiculotubular appendage material (arrowhead) in the process of being sloughed off the plasmalemma as it becomes more homogeneously dense. Bar = 0.5 μm. 11. Four sporoblasts with homogeneously thickened plasmalemma (after last cell division) and lacking attached appendages visible in the surrounding cytoplasm (arrowheads). Note the presence of the forming polar filament (PF). Bar = 0.5 μm. 12–13. Typical spores of B. algerae with 9–11 polar filament coils. 12. Early activated spore. Note the translocation of some polar filament coils. Bar = 0.5 μm. 13. Mature resting spore. Bar = 0.5 μm.
    Figure Legend Snippet: Electron microscopic overview of infected human muscle biopsy tissue containing developmental stages of the microsporidial parasite, Brachiola algerae (Hba). 2. Early proliferative stages of the parasite in direct contact with the host muscle-cell cytoplasm (H). Note the presence of vesiculotubular appendages (arrow) attached to the parasite thickened plasmalemma, forming many dense bodies (arrowhead) in the host cytoplasm surrounding the parasite cells and abutting the host myofilaments (M). Bar = 1 μm. 3. Diplokaryotic proliferative parasite cells and spores (S). Note the attachment of the vesiculotubular appendages (arrows) to the proliferative cells but not to the spores. Dense appendage material is present in the space between parasite cells (arrowheads). Bar = 1 μm. 4. Low-power electron micrograph of clustered parasites in host cell cytoplasm (H). Host nuclei (N) and cytoplasm in direct contact with parasite cells and dense vesiculotubular appendage material (arrowheads) scattered throughout host cytoplasm. Note the lack of myofilaments between parasite cells. Bar = 1 μm. 5. All parasite stages are present and intermixed, an indication of asynchronous development. Cytokinesis and karyokinesis are linked, resulting in individual organisms containing one diplokaryon (D) or two with indications of cell division (*). Bar = 1 μm. 6. Proliferative cell containing a diplokaryon with condensed chromatin (arrows) and a spindle plaque (PL), indicating karyokinetic activity. Bar = 1 μm. 7. Proliferative cell containing a diplokaryon with condensed chromatin (arrows), extensive ER, and attached dense vesiculotubular appendages (arrowhead). Bar = 0.5 μm. 8–10. Sporonts. Note the change in appearance of the appendages (arrowhead) to clumps of dense material detaching from the plasmalemma. 8. Early sporont. Note the relatively lower cytoplasmic density when compared to the other two. Bar = 0.5 μm. 9. Note the presence of condensing chromatin in the sporont nucleus; it indicates the disporous nature of this parasite. Bar = 0.5 μm. 10. Note the abundance of dense vesiculotubular appendage material (arrowhead) in the process of being sloughed off the plasmalemma as it becomes more homogeneously dense. Bar = 0.5 μm. 11. Four sporoblasts with homogeneously thickened plasmalemma (after last cell division) and lacking attached appendages visible in the surrounding cytoplasm (arrowheads). Note the presence of the forming polar filament (PF). Bar = 0.5 μm. 12–13. Typical spores of B. algerae with 9–11 polar filament coils. 12. Early activated spore. Note the translocation of some polar filament coils. Bar = 0.5 μm. 13. Mature resting spore. Bar = 0.5 μm.

    Techniques Used: Infection, Activity Assay, Translocation Assay

    Human (Hba) isolate of Brachiola algerae grown in rat muscle (L6E9) cell line. 14. Diplokaryotic proliferative (P) and sporogonic parasite cells, including sporonts (SP), sporoblast (SB), and spore (S) cells in direct contact with cell cytoplasm (H). Note the presence of detached appendage material (arrowheads) in the surrounding cytoplasm. Bar = 0.5 μm. 15. Proliferative (P) and sporogonic parasite cells. Appendages (arrows) are attached to the surface of proliferative cells and the spores (S) and sporoblasts (SB) have a uniformly thick surface coating that lacks appendages, ridges or general irregularities. Detached appendage material (arrowheads) is clustered in the surrounding cytoplasm. Bar = 0.5 μm. 16. Low power of infected host cell demonstrating the abundance of parasites filling the entire cell. Note the presence of all stages of development and the presence of dense appendage material scattered in the host cell cytoplasm. Bar = 10 μm. 17. Sporoblasts containing forming polar filaments. Large clusters of detached appendage material (arrowheads) are in the surrounding cytoplasm. Bar = 0.5 μm. 18. Spores. Mature elongate spore containing approximately 9–10 coils of polar filament arranged in a single row surrounding diplokaryotic nuclei. Bar = 1 μm.
    Figure Legend Snippet: Human (Hba) isolate of Brachiola algerae grown in rat muscle (L6E9) cell line. 14. Diplokaryotic proliferative (P) and sporogonic parasite cells, including sporonts (SP), sporoblast (SB), and spore (S) cells in direct contact with cell cytoplasm (H). Note the presence of detached appendage material (arrowheads) in the surrounding cytoplasm. Bar = 0.5 μm. 15. Proliferative (P) and sporogonic parasite cells. Appendages (arrows) are attached to the surface of proliferative cells and the spores (S) and sporoblasts (SB) have a uniformly thick surface coating that lacks appendages, ridges or general irregularities. Detached appendage material (arrowheads) is clustered in the surrounding cytoplasm. Bar = 0.5 μm. 16. Low power of infected host cell demonstrating the abundance of parasites filling the entire cell. Note the presence of all stages of development and the presence of dense appendage material scattered in the host cell cytoplasm. Bar = 10 μm. 17. Sporoblasts containing forming polar filaments. Large clusters of detached appendage material (arrowheads) are in the surrounding cytoplasm. Bar = 0.5 μm. 18. Spores. Mature elongate spore containing approximately 9–10 coils of polar filament arranged in a single row surrounding diplokaryotic nuclei. Bar = 1 μm.

    Techniques Used: Infection



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    Electron microscopic overview of infected human muscle biopsy tissue containing developmental stages of the microsporidial parasite, Brachiola <t>algerae</t> <t>(Hba).</t> 2. Early proliferative stages of the parasite in direct contact with the host muscle-cell cytoplasm (H). Note the presence of vesiculotubular appendages (arrow) attached to the parasite thickened plasmalemma, forming many dense bodies (arrowhead) in the host cytoplasm surrounding the parasite cells and abutting the host myofilaments (M). Bar = 1 μm. 3. Diplokaryotic proliferative parasite cells and spores (S). Note the attachment of the vesiculotubular appendages (arrows) to the proliferative cells but not to the spores. Dense appendage material is present in the space between parasite cells (arrowheads). Bar = 1 μm. 4. Low-power electron micrograph of clustered parasites in host cell cytoplasm (H). Host nuclei (N) and cytoplasm in direct contact with parasite cells and dense vesiculotubular appendage material (arrowheads) scattered throughout host cytoplasm. Note the lack of myofilaments between parasite cells. Bar = 1 μm. 5. All parasite stages are present and intermixed, an indication of asynchronous development. Cytokinesis and karyokinesis are linked, resulting in individual organisms containing one diplokaryon (D) or two with indications of cell division (*). Bar = 1 μm. 6. Proliferative cell containing a diplokaryon with condensed chromatin (arrows) and a spindle plaque (PL), indicating karyokinetic activity. Bar = 1 μm. 7. Proliferative cell containing a diplokaryon with condensed chromatin (arrows), extensive ER, and attached dense vesiculotubular appendages (arrowhead). Bar = 0.5 μm. 8–10. Sporonts. Note the change in appearance of the appendages (arrowhead) to clumps of dense material detaching from the plasmalemma. 8. Early sporont. Note the relatively lower cytoplasmic density when compared to the other two. Bar = 0.5 μm. 9. Note the presence of condensing chromatin in the sporont nucleus; it indicates the disporous nature of this parasite. Bar = 0.5 μm. 10. Note the abundance of dense vesiculotubular appendage material (arrowhead) in the process of being sloughed off the plasmalemma as it becomes more homogeneously dense. Bar = 0.5 μm. 11. Four sporoblasts with homogeneously thickened plasmalemma (after last cell division) and lacking attached appendages visible in the surrounding cytoplasm (arrowheads). Note the presence of the forming polar filament (PF). Bar = 0.5 μm. 12–13. Typical spores of B. algerae with 9–11 polar filament coils. 12. Early activated spore. Note the translocation of some polar filament coils. Bar = 0.5 μm. 13. Mature resting spore. Bar = 0.5 μm.
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    Electron microscopic overview of infected human muscle biopsy tissue containing developmental stages of the microsporidial parasite, Brachiola algerae (Hba). 2. Early proliferative stages of the parasite in direct contact with the host muscle-cell cytoplasm (H). Note the presence of vesiculotubular appendages (arrow) attached to the parasite thickened plasmalemma, forming many dense bodies (arrowhead) in the host cytoplasm surrounding the parasite cells and abutting the host myofilaments (M). Bar = 1 μm. 3. Diplokaryotic proliferative parasite cells and spores (S). Note the attachment of the vesiculotubular appendages (arrows) to the proliferative cells but not to the spores. Dense appendage material is present in the space between parasite cells (arrowheads). Bar = 1 μm. 4. Low-power electron micrograph of clustered parasites in host cell cytoplasm (H). Host nuclei (N) and cytoplasm in direct contact with parasite cells and dense vesiculotubular appendage material (arrowheads) scattered throughout host cytoplasm. Note the lack of myofilaments between parasite cells. Bar = 1 μm. 5. All parasite stages are present and intermixed, an indication of asynchronous development. Cytokinesis and karyokinesis are linked, resulting in individual organisms containing one diplokaryon (D) or two with indications of cell division (*). Bar = 1 μm. 6. Proliferative cell containing a diplokaryon with condensed chromatin (arrows) and a spindle plaque (PL), indicating karyokinetic activity. Bar = 1 μm. 7. Proliferative cell containing a diplokaryon with condensed chromatin (arrows), extensive ER, and attached dense vesiculotubular appendages (arrowhead). Bar = 0.5 μm. 8–10. Sporonts. Note the change in appearance of the appendages (arrowhead) to clumps of dense material detaching from the plasmalemma. 8. Early sporont. Note the relatively lower cytoplasmic density when compared to the other two. Bar = 0.5 μm. 9. Note the presence of condensing chromatin in the sporont nucleus; it indicates the disporous nature of this parasite. Bar = 0.5 μm. 10. Note the abundance of dense vesiculotubular appendage material (arrowhead) in the process of being sloughed off the plasmalemma as it becomes more homogeneously dense. Bar = 0.5 μm. 11. Four sporoblasts with homogeneously thickened plasmalemma (after last cell division) and lacking attached appendages visible in the surrounding cytoplasm (arrowheads). Note the presence of the forming polar filament (PF). Bar = 0.5 μm. 12–13. Typical spores of B. algerae with 9–11 polar filament coils. 12. Early activated spore. Note the translocation of some polar filament coils. Bar = 0.5 μm. 13. Mature resting spore. Bar = 0.5 μm.

    Journal: The Journal of eukaryotic microbiology

    Article Title: An Analysis of the Microsporidian Genus Brachiola , with Comparisons of Human and Insect Isolates of Brachiola algerae

    doi:

    Figure Lengend Snippet: Electron microscopic overview of infected human muscle biopsy tissue containing developmental stages of the microsporidial parasite, Brachiola algerae (Hba). 2. Early proliferative stages of the parasite in direct contact with the host muscle-cell cytoplasm (H). Note the presence of vesiculotubular appendages (arrow) attached to the parasite thickened plasmalemma, forming many dense bodies (arrowhead) in the host cytoplasm surrounding the parasite cells and abutting the host myofilaments (M). Bar = 1 μm. 3. Diplokaryotic proliferative parasite cells and spores (S). Note the attachment of the vesiculotubular appendages (arrows) to the proliferative cells but not to the spores. Dense appendage material is present in the space between parasite cells (arrowheads). Bar = 1 μm. 4. Low-power electron micrograph of clustered parasites in host cell cytoplasm (H). Host nuclei (N) and cytoplasm in direct contact with parasite cells and dense vesiculotubular appendage material (arrowheads) scattered throughout host cytoplasm. Note the lack of myofilaments between parasite cells. Bar = 1 μm. 5. All parasite stages are present and intermixed, an indication of asynchronous development. Cytokinesis and karyokinesis are linked, resulting in individual organisms containing one diplokaryon (D) or two with indications of cell division (*). Bar = 1 μm. 6. Proliferative cell containing a diplokaryon with condensed chromatin (arrows) and a spindle plaque (PL), indicating karyokinetic activity. Bar = 1 μm. 7. Proliferative cell containing a diplokaryon with condensed chromatin (arrows), extensive ER, and attached dense vesiculotubular appendages (arrowhead). Bar = 0.5 μm. 8–10. Sporonts. Note the change in appearance of the appendages (arrowhead) to clumps of dense material detaching from the plasmalemma. 8. Early sporont. Note the relatively lower cytoplasmic density when compared to the other two. Bar = 0.5 μm. 9. Note the presence of condensing chromatin in the sporont nucleus; it indicates the disporous nature of this parasite. Bar = 0.5 μm. 10. Note the abundance of dense vesiculotubular appendage material (arrowhead) in the process of being sloughed off the plasmalemma as it becomes more homogeneously dense. Bar = 0.5 μm. 11. Four sporoblasts with homogeneously thickened plasmalemma (after last cell division) and lacking attached appendages visible in the surrounding cytoplasm (arrowheads). Note the presence of the forming polar filament (PF). Bar = 0.5 μm. 12–13. Typical spores of B. algerae with 9–11 polar filament coils. 12. Early activated spore. Note the translocation of some polar filament coils. Bar = 0.5 μm. 13. Mature resting spore. Bar = 0.5 μm.

    Article Snippet: The human isolate of B. algerae (ATCC-PRA109) will be referred to as Hba.

    Techniques: Infection, Activity Assay, Translocation Assay

    Human (Hba) isolate of Brachiola algerae grown in rat muscle (L6E9) cell line. 14. Diplokaryotic proliferative (P) and sporogonic parasite cells, including sporonts (SP), sporoblast (SB), and spore (S) cells in direct contact with cell cytoplasm (H). Note the presence of detached appendage material (arrowheads) in the surrounding cytoplasm. Bar = 0.5 μm. 15. Proliferative (P) and sporogonic parasite cells. Appendages (arrows) are attached to the surface of proliferative cells and the spores (S) and sporoblasts (SB) have a uniformly thick surface coating that lacks appendages, ridges or general irregularities. Detached appendage material (arrowheads) is clustered in the surrounding cytoplasm. Bar = 0.5 μm. 16. Low power of infected host cell demonstrating the abundance of parasites filling the entire cell. Note the presence of all stages of development and the presence of dense appendage material scattered in the host cell cytoplasm. Bar = 10 μm. 17. Sporoblasts containing forming polar filaments. Large clusters of detached appendage material (arrowheads) are in the surrounding cytoplasm. Bar = 0.5 μm. 18. Spores. Mature elongate spore containing approximately 9–10 coils of polar filament arranged in a single row surrounding diplokaryotic nuclei. Bar = 1 μm.

    Journal: The Journal of eukaryotic microbiology

    Article Title: An Analysis of the Microsporidian Genus Brachiola , with Comparisons of Human and Insect Isolates of Brachiola algerae

    doi:

    Figure Lengend Snippet: Human (Hba) isolate of Brachiola algerae grown in rat muscle (L6E9) cell line. 14. Diplokaryotic proliferative (P) and sporogonic parasite cells, including sporonts (SP), sporoblast (SB), and spore (S) cells in direct contact with cell cytoplasm (H). Note the presence of detached appendage material (arrowheads) in the surrounding cytoplasm. Bar = 0.5 μm. 15. Proliferative (P) and sporogonic parasite cells. Appendages (arrows) are attached to the surface of proliferative cells and the spores (S) and sporoblasts (SB) have a uniformly thick surface coating that lacks appendages, ridges or general irregularities. Detached appendage material (arrowheads) is clustered in the surrounding cytoplasm. Bar = 0.5 μm. 16. Low power of infected host cell demonstrating the abundance of parasites filling the entire cell. Note the presence of all stages of development and the presence of dense appendage material scattered in the host cell cytoplasm. Bar = 10 μm. 17. Sporoblasts containing forming polar filaments. Large clusters of detached appendage material (arrowheads) are in the surrounding cytoplasm. Bar = 0.5 μm. 18. Spores. Mature elongate spore containing approximately 9–10 coils of polar filament arranged in a single row surrounding diplokaryotic nuclei. Bar = 1 μm.

    Article Snippet: The human isolate of B. algerae (ATCC-PRA109) will be referred to as Hba.

    Techniques: Infection