azide peg3 biotin conjugate  (Millipore)


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    Structured Review

    Millipore azide peg3 biotin conjugate
    Azide Peg3 Biotin Conjugate, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/azide peg3 biotin conjugate/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    azide peg3 biotin conjugate - by Bioz Stars, 2019-12
    94/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Large-scale forward genetics screening identifies Trpa1 as a chemosensor for predator odor-evoked innate fear behaviors
    Article Snippet: For click chemistry reaction, 2 µl of 50 mM CuSO4 solution was mixed with 2 µl of 2.5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA, Sigma-Aldrich, USA), followed by addition of 1 µl of 1.25 mM azide-PEG3-biotin conjugate (Sigma-Aldrich, USA) and 1 µl of 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA). .. For click chemistry reaction, 2 µl of 50 mM CuSO4 solution was mixed with 2 µl of 2.5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA, Sigma-Aldrich, USA), followed by addition of 1 µl of 1.25 mM azide-PEG3-biotin conjugate (Sigma-Aldrich, USA) and 1 µl of 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA).

    Transfection:

    Article Title: Large-scale forward genetics screening identifies Trpa1 as a chemosensor for predator odor-evoked innate fear behaviors
    Article Snippet: After 24 h of transfection, the cells were washed with 500 µl calcium solution (125 mM NaCl, 2 mM MgCl2 , 4.5 mM KCl, 10 mM glucose, 20 mM HEPES, 2 mM CaCl2 , 0.1% DMSO, pH 7.2), and incubated with 300 µM alkynyl-TMT in calcium solution at 30 °C for 6 min. After washing again with calcium solution, the cells were lysed with lysis buffer (50 mM HEPES, 2 mM MgCl2 , 10 mM KCl, 1% SDS, 150 units/ml of Benzonase (Sigma-Aldrich, USA) pH 8.0, supplemented with complete™, Mini, EDTA-free protease inhibitor cocktail (Sigma-Aldrich, USA)) for 20 min at room temperature. .. For click chemistry reaction, 2 µl of 50 mM CuSO4 solution was mixed with 2 µl of 2.5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA, Sigma-Aldrich, USA), followed by addition of 1 µl of 1.25 mM azide-PEG3-biotin conjugate (Sigma-Aldrich, USA) and 1 µl of 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA).

    Synthesized:

    Article Title: Astrocytes and oligodendrocytes in grey and white matter regions of the brain metabolize fatty acids
    Article Snippet: Alkyne stearate (17-octadecynoic acid), azide-PEG3-biotin conjugate, [acetonitrile]4 CuBF4 and DAPI were obtained from Sigma Aldrich, lipid free BSA from Applichem. .. Alkyne stearate (17-octadecynoic acid), azide-PEG3-biotin conjugate, [acetonitrile]4 CuBF4 and DAPI were obtained from Sigma Aldrich, lipid free BSA from Applichem.

    Protease Inhibitor:

    Article Title: Large-scale forward genetics screening identifies Trpa1 as a chemosensor for predator odor-evoked innate fear behaviors
    Article Snippet: After 24 h of transfection, the cells were washed with 500 µl calcium solution (125 mM NaCl, 2 mM MgCl2 , 4.5 mM KCl, 10 mM glucose, 20 mM HEPES, 2 mM CaCl2 , 0.1% DMSO, pH 7.2), and incubated with 300 µM alkynyl-TMT in calcium solution at 30 °C for 6 min. After washing again with calcium solution, the cells were lysed with lysis buffer (50 mM HEPES, 2 mM MgCl2 , 10 mM KCl, 1% SDS, 150 units/ml of Benzonase (Sigma-Aldrich, USA) pH 8.0, supplemented with complete™, Mini, EDTA-free protease inhibitor cocktail (Sigma-Aldrich, USA)) for 20 min at room temperature. .. For click chemistry reaction, 2 µl of 50 mM CuSO4 solution was mixed with 2 µl of 2.5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA, Sigma-Aldrich, USA), followed by addition of 1 µl of 1.25 mM azide-PEG3-biotin conjugate (Sigma-Aldrich, USA) and 1 µl of 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA).

    Article Title: A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite
    Article Snippet: The use of DPE combined with the subsequent introduction with an azide-linked biotin by the CuAAC reaction is expected to enable the highly effective tagging of the DOPAC modified protein. .. 2.1 DOPAC, laccase from Rhus vernicifera , glyceraldehyde-3-phosphate dehydrogenase (GAPDH), tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA), bis(4-nitrophenyl) phosphate (BNPP) and azide-PEG3 -biotin conjugate were obtained from Sigma Aldrich (St. Louis, USA). p -Toluensulfonic acid monohydrate (PTSA), n -butanol, toluene, copper (II) sulfate pentahydrate, protease inhibitor cocktail and Chemi-Lumi One Super were purchased from nacalai tasque (Kyoto, Japan). .. 2-Propyl-1-ol was obtained from Tokyo Chemical Industry (Tokyo, Japan).

    BIA-KA:

    Article Title: Large-scale forward genetics screening identifies Trpa1 as a chemosensor for predator odor-evoked innate fear behaviors
    Article Snippet: The lysate was centrifuged at 12,000 × g for 3 min to pellet cell debris and the protein concentration was measured by BCA assay (Thermo Fisher Scientific, MA, USA). .. For click chemistry reaction, 2 µl of 50 mM CuSO4 solution was mixed with 2 µl of 2.5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA, Sigma-Aldrich, USA), followed by addition of 1 µl of 1.25 mM azide-PEG3-biotin conjugate (Sigma-Aldrich, USA) and 1 µl of 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA).

    Construct:

    Article Title: Large-scale forward genetics screening identifies Trpa1 as a chemosensor for predator odor-evoked innate fear behaviors
    Article Snippet: Approximately 1 × 105 HEK293T cells were seeded into a 12-well plate and incubated in DMEM medium containing 10% FBS and 1% Penicillin/Streptomycin at 37 °C for 18 h. For transient transfection, 1 µg of pHA-Trpa1-P2A-mCherry construct was mixed with 3 µl Fugene 6 in 60 µl OptiMEM per 12 well. .. For click chemistry reaction, 2 µl of 50 mM CuSO4 solution was mixed with 2 µl of 2.5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA, Sigma-Aldrich, USA), followed by addition of 1 µl of 1.25 mM azide-PEG3-biotin conjugate (Sigma-Aldrich, USA) and 1 µl of 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA).

    Hemagglutination Assay:

    Article Title: Large-scale forward genetics screening identifies Trpa1 as a chemosensor for predator odor-evoked innate fear behaviors
    Article Snippet: Paragraph title: Streptavidin pulldown of alkynyl-TMT-modified HA-Trpa1 ... For click chemistry reaction, 2 µl of 50 mM CuSO4 solution was mixed with 2 µl of 2.5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA, Sigma-Aldrich, USA), followed by addition of 1 µl of 1.25 mM azide-PEG3-biotin conjugate (Sigma-Aldrich, USA) and 1 µl of 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA).

    Incubation:

    Article Title: Large-scale forward genetics screening identifies Trpa1 as a chemosensor for predator odor-evoked innate fear behaviors
    Article Snippet: After 24 h of transfection, the cells were washed with 500 µl calcium solution (125 mM NaCl, 2 mM MgCl2 , 4.5 mM KCl, 10 mM glucose, 20 mM HEPES, 2 mM CaCl2 , 0.1% DMSO, pH 7.2), and incubated with 300 µM alkynyl-TMT in calcium solution at 30 °C for 6 min. After washing again with calcium solution, the cells were lysed with lysis buffer (50 mM HEPES, 2 mM MgCl2 , 10 mM KCl, 1% SDS, 150 units/ml of Benzonase (Sigma-Aldrich, USA) pH 8.0, supplemented with complete™, Mini, EDTA-free protease inhibitor cocktail (Sigma-Aldrich, USA)) for 20 min at room temperature. .. For click chemistry reaction, 2 µl of 50 mM CuSO4 solution was mixed with 2 µl of 2.5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA, Sigma-Aldrich, USA), followed by addition of 1 µl of 1.25 mM azide-PEG3-biotin conjugate (Sigma-Aldrich, USA) and 1 µl of 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA).

    other:

    Article Title: A novel alkyne cholesterol to trace cellular cholesterol metabolism and localization
    Article Snippet: Filipin and azide-PEG3-biotin conjugate were obtained from Sigma.

    Protein Concentration:

    Article Title: Large-scale forward genetics screening identifies Trpa1 as a chemosensor for predator odor-evoked innate fear behaviors
    Article Snippet: The lysate was centrifuged at 12,000 × g for 3 min to pellet cell debris and the protein concentration was measured by BCA assay (Thermo Fisher Scientific, MA, USA). .. For click chemistry reaction, 2 µl of 50 mM CuSO4 solution was mixed with 2 µl of 2.5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA, Sigma-Aldrich, USA), followed by addition of 1 µl of 1.25 mM azide-PEG3-biotin conjugate (Sigma-Aldrich, USA) and 1 µl of 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA).

    Lysis:

    Article Title: Large-scale forward genetics screening identifies Trpa1 as a chemosensor for predator odor-evoked innate fear behaviors
    Article Snippet: After 24 h of transfection, the cells were washed with 500 µl calcium solution (125 mM NaCl, 2 mM MgCl2 , 4.5 mM KCl, 10 mM glucose, 20 mM HEPES, 2 mM CaCl2 , 0.1% DMSO, pH 7.2), and incubated with 300 µM alkynyl-TMT in calcium solution at 30 °C for 6 min. After washing again with calcium solution, the cells were lysed with lysis buffer (50 mM HEPES, 2 mM MgCl2 , 10 mM KCl, 1% SDS, 150 units/ml of Benzonase (Sigma-Aldrich, USA) pH 8.0, supplemented with complete™, Mini, EDTA-free protease inhibitor cocktail (Sigma-Aldrich, USA)) for 20 min at room temperature. .. For click chemistry reaction, 2 µl of 50 mM CuSO4 solution was mixed with 2 µl of 2.5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA, Sigma-Aldrich, USA), followed by addition of 1 µl of 1.25 mM azide-PEG3-biotin conjugate (Sigma-Aldrich, USA) and 1 µl of 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA).