azide peg3 biotin conjugate  (Jena Bioscience)


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    Name:
    Azide PEG3 Biotin Conjugate
    Description:
    Azide PEG3 Biotin is suitable for the introduction of a biotin moiety to terminal Alkyne and strained Alkyne e g DBCO labeled biomolecules via Cu I catalyzed Alknye Azide CUAAC or Cu I free strain promoted Alkyne Azide Click Chemistry SPAAC reaction respectively The hydrophilic PEG3 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP labeled streptavidin or its affinity purification via streptavidin agarose Due to the extremely high affinity of biotin towards streptavidin KD 10 15 M the biotinylated molecule streptavidin interaction is essentially irreversible under physiological conditions
    Catalog Number:
    clk-az104p4-100
    Molecular Weight:
    444.54 g/mol
    Price:
    293.66
    Purity:
    > 95 % (H NMR)
    Category:
    Click Chemistry
    Format:
    off-white to grey solid
    Formula:
    C18H32N6O5S
    Buy from Supplier


    Structured Review

    Jena Bioscience azide peg3 biotin conjugate
    Azide PEG3 Biotin is suitable for the introduction of a biotin moiety to terminal Alkyne and strained Alkyne e g DBCO labeled biomolecules via Cu I catalyzed Alknye Azide CUAAC or Cu I free strain promoted Alkyne Azide Click Chemistry SPAAC reaction respectively The hydrophilic PEG3 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP labeled streptavidin or its affinity purification via streptavidin agarose Due to the extremely high affinity of biotin towards streptavidin KD 10 15 M the biotinylated molecule streptavidin interaction is essentially irreversible under physiological conditions
    https://www.bioz.com/result/azide peg3 biotin conjugate/product/Jena Bioscience
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    azide peg3 biotin conjugate - by Bioz Stars, 2020-01
    85/100 stars

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    Related Articles

    UDS Assay:

    Article Title: Amplification of unscheduled DNA synthesis signal enables fluorescence-based single cell quantification of transcription-coupled nucleotide excision repair
    Article Snippet: .. Amplified UDS assay After blocking, endogenous peroxidases were quenched to reduce non-specific activation of tyramide using 3% hydrogen peroxide solution (Sigma-Aldrich) in PBS for 1 h. Subsequently, DNA was denaturated using freshly prepared 0.07 M NaOH in PBS for 5 min at room temperature, after which samples were incubated with PBS containing 0.5% BSA and 0.15% glycine for 1 h. The Click-it reaction was performed using 100 μl of the Click it reaction cocktail containing Azide-PEG3-Biotin Conjugate (20 μM in DMSO, Jena Bioscience), 1× Click-it reaction buffer (ThermoFisher Scientific), copper(III) sulfate (0.1 M) and 10× reaction buffer additive (ThermoFisher Scientific). .. Subsequently, for tyramide-based amplification, samples were incubated with 100 μl HRP–streptavidin conjugate (500 μg/ml) in 1% BSA for 1 h. Subsequently, samples were incubated for 10 min at room temperature with 100 μl amplification buffer containing 0.0015% H2 O2 and 1:100 dilution of Alexa-Fluor 488 nm labeled tyramide, which was dissolved in 150 μl DMSO (ThermoFisher Scientific).

    Amplification:

    Article Title: Biotinylation of Deoxyguanosine at the Abasic Site in Double-Stranded Oligodeoxynucleotides
    Article Snippet: As a control sample, the double-stranded DNA oligonucleotides DS-G/C without an abasic site was prepared by the PCR amplification with a set of primers, FW primer, 5′-(CAG TGA AGT TGG CAG ACT GAG C)-3′, and Rev primer, 5′-(CTG ACT TGC CAC CTA TAG ACA GC)-3′, and the single-stranded DNA oligonucleotides SS-G as the template. .. A mixture of 30 μ L (0.1 M CuBr/0.1 M TBTA 1 : 2 in DMSO/t-BuOH 3 : 1) and 20 μ L of Biotin-PEG3 -Azide solution (Jena Bioscience, USA) was added to 50 μ L of the solution of DNA in Tris buffer.

    Article Title: Amplification of unscheduled DNA synthesis signal enables fluorescence-based single cell quantification of transcription-coupled nucleotide excision repair
    Article Snippet: .. Amplified UDS assay After blocking, endogenous peroxidases were quenched to reduce non-specific activation of tyramide using 3% hydrogen peroxide solution (Sigma-Aldrich) in PBS for 1 h. Subsequently, DNA was denaturated using freshly prepared 0.07 M NaOH in PBS for 5 min at room temperature, after which samples were incubated with PBS containing 0.5% BSA and 0.15% glycine for 1 h. The Click-it reaction was performed using 100 μl of the Click it reaction cocktail containing Azide-PEG3-Biotin Conjugate (20 μM in DMSO, Jena Bioscience), 1× Click-it reaction buffer (ThermoFisher Scientific), copper(III) sulfate (0.1 M) and 10× reaction buffer additive (ThermoFisher Scientific). .. Subsequently, for tyramide-based amplification, samples were incubated with 100 μl HRP–streptavidin conjugate (500 μg/ml) in 1% BSA for 1 h. Subsequently, samples were incubated for 10 min at room temperature with 100 μl amplification buffer containing 0.0015% H2 O2 and 1:100 dilution of Alexa-Fluor 488 nm labeled tyramide, which was dissolved in 150 μl DMSO (ThermoFisher Scientific).

    Sonication:

    Article Title: The oncoprotein DEK affects the outcome of PARP1/2 inhibition during mild replication stress
    Article Snippet: .. Click reaction to label EdU-containing DNA was performed using biotin-PEG3-azide (Jena Bioscience) for 90 min and cells were sonicated in a Bioruptor sonicator (Diogenode) to solubilize chromatin fragments. .. Biotin-linked fragments were precipitated overnight at 4°C using streptavidin-coupled magnetic beads (0.8 μm, Solulink).

    Magnetic Beads:

    Article Title: The oncoprotein DEK affects the outcome of PARP1/2 inhibition during mild replication stress
    Article Snippet: Click reaction to label EdU-containing DNA was performed using biotin-PEG3-azide (Jena Bioscience) for 90 min and cells were sonicated in a Bioruptor sonicator (Diogenode) to solubilize chromatin fragments. .. Biotin-linked fragments were precipitated overnight at 4°C using streptavidin-coupled magnetic beads (0.8 μm, Solulink).

    Isolation:

    Article Title: The oncoprotein DEK affects the outcome of PARP1/2 inhibition during mild replication stress
    Article Snippet: Paragraph title: Isolation of proteins on nascent DNA (iPOND) ... Click reaction to label EdU-containing DNA was performed using biotin-PEG3-azide (Jena Bioscience) for 90 min and cells were sonicated in a Bioruptor sonicator (Diogenode) to solubilize chromatin fragments.

    Blocking Assay:

    Article Title: Amplification of unscheduled DNA synthesis signal enables fluorescence-based single cell quantification of transcription-coupled nucleotide excision repair
    Article Snippet: .. Amplified UDS assay After blocking, endogenous peroxidases were quenched to reduce non-specific activation of tyramide using 3% hydrogen peroxide solution (Sigma-Aldrich) in PBS for 1 h. Subsequently, DNA was denaturated using freshly prepared 0.07 M NaOH in PBS for 5 min at room temperature, after which samples were incubated with PBS containing 0.5% BSA and 0.15% glycine for 1 h. The Click-it reaction was performed using 100 μl of the Click it reaction cocktail containing Azide-PEG3-Biotin Conjugate (20 μM in DMSO, Jena Bioscience), 1× Click-it reaction buffer (ThermoFisher Scientific), copper(III) sulfate (0.1 M) and 10× reaction buffer additive (ThermoFisher Scientific). .. Subsequently, for tyramide-based amplification, samples were incubated with 100 μl HRP–streptavidin conjugate (500 μg/ml) in 1% BSA for 1 h. Subsequently, samples were incubated for 10 min at room temperature with 100 μl amplification buffer containing 0.0015% H2 O2 and 1:100 dilution of Alexa-Fluor 488 nm labeled tyramide, which was dissolved in 150 μl DMSO (ThermoFisher Scientific).

    Purification:

    Article Title: Biotinylation of Deoxyguanosine at the Abasic Site in Double-Stranded Oligodeoxynucleotides
    Article Snippet: The reaction mixture was purified on G-50 micro column (GE healthcare, USA). .. A mixture of 30 μ L (0.1 M CuBr/0.1 M TBTA 1 : 2 in DMSO/t-BuOH 3 : 1) and 20 μ L of Biotin-PEG3 -Azide solution (Jena Bioscience, USA) was added to 50 μ L of the solution of DNA in Tris buffer.

    Activation Assay:

    Article Title: Amplification of unscheduled DNA synthesis signal enables fluorescence-based single cell quantification of transcription-coupled nucleotide excision repair
    Article Snippet: .. Amplified UDS assay After blocking, endogenous peroxidases were quenched to reduce non-specific activation of tyramide using 3% hydrogen peroxide solution (Sigma-Aldrich) in PBS for 1 h. Subsequently, DNA was denaturated using freshly prepared 0.07 M NaOH in PBS for 5 min at room temperature, after which samples were incubated with PBS containing 0.5% BSA and 0.15% glycine for 1 h. The Click-it reaction was performed using 100 μl of the Click it reaction cocktail containing Azide-PEG3-Biotin Conjugate (20 μM in DMSO, Jena Bioscience), 1× Click-it reaction buffer (ThermoFisher Scientific), copper(III) sulfate (0.1 M) and 10× reaction buffer additive (ThermoFisher Scientific). .. Subsequently, for tyramide-based amplification, samples were incubated with 100 μl HRP–streptavidin conjugate (500 μg/ml) in 1% BSA for 1 h. Subsequently, samples were incubated for 10 min at room temperature with 100 μl amplification buffer containing 0.0015% H2 O2 and 1:100 dilution of Alexa-Fluor 488 nm labeled tyramide, which was dissolved in 150 μl DMSO (ThermoFisher Scientific).

    Incubation:

    Article Title: Biotinylation of Deoxyguanosine at the Abasic Site in Double-Stranded Oligodeoxynucleotides
    Article Snippet: A mixture of 30 μ L (0.1 M CuBr/0.1 M TBTA 1 : 2 in DMSO/t-BuOH 3 : 1) and 20 μ L of Biotin-PEG3 -Azide solution (Jena Bioscience, USA) was added to 50 μ L of the solution of DNA in Tris buffer. .. The reaction was incubated at 37°C for 2 h. To remove the excess click reagents, the biotinylated DNA was purified on NAP-5 columns (GE Healthcare, USA).

    Article Title: Amplification of unscheduled DNA synthesis signal enables fluorescence-based single cell quantification of transcription-coupled nucleotide excision repair
    Article Snippet: .. Amplified UDS assay After blocking, endogenous peroxidases were quenched to reduce non-specific activation of tyramide using 3% hydrogen peroxide solution (Sigma-Aldrich) in PBS for 1 h. Subsequently, DNA was denaturated using freshly prepared 0.07 M NaOH in PBS for 5 min at room temperature, after which samples were incubated with PBS containing 0.5% BSA and 0.15% glycine for 1 h. The Click-it reaction was performed using 100 μl of the Click it reaction cocktail containing Azide-PEG3-Biotin Conjugate (20 μM in DMSO, Jena Bioscience), 1× Click-it reaction buffer (ThermoFisher Scientific), copper(III) sulfate (0.1 M) and 10× reaction buffer additive (ThermoFisher Scientific). .. Subsequently, for tyramide-based amplification, samples were incubated with 100 μl HRP–streptavidin conjugate (500 μg/ml) in 1% BSA for 1 h. Subsequently, samples were incubated for 10 min at room temperature with 100 μl amplification buffer containing 0.0015% H2 O2 and 1:100 dilution of Alexa-Fluor 488 nm labeled tyramide, which was dissolved in 150 μl DMSO (ThermoFisher Scientific).

    Article Title: The oncoprotein DEK affects the outcome of PARP1/2 inhibition during mild replication stress
    Article Snippet: At least 1x108 HeLa S3 cells per sample were pulsed with 10 μM EdU (Thermo Fisher Scientific) for the indicated times and either incubated with 10 μM thymidine (Merck) for 0–30 min before fixation (chase experiments) or fixated immediately (pulse experiments). .. Click reaction to label EdU-containing DNA was performed using biotin-PEG3-azide (Jena Bioscience) for 90 min and cells were sonicated in a Bioruptor sonicator (Diogenode) to solubilize chromatin fragments.

    Polymerase Chain Reaction:

    Article Title: Biotinylation of Deoxyguanosine at the Abasic Site in Double-Stranded Oligodeoxynucleotides
    Article Snippet: The double-stranded DNA oligonucleotides DS-G/C were also purified with NucleoSpin Gel and PCR Clean-up kit. .. A mixture of 30 μ L (0.1 M CuBr/0.1 M TBTA 1 : 2 in DMSO/t-BuOH 3 : 1) and 20 μ L of Biotin-PEG3 -Azide solution (Jena Bioscience, USA) was added to 50 μ L of the solution of DNA in Tris buffer.

    Labeling:

    Article Title: Amplification of unscheduled DNA synthesis signal enables fluorescence-based single cell quantification of transcription-coupled nucleotide excision repair
    Article Snippet: Amplified UDS assay After blocking, endogenous peroxidases were quenched to reduce non-specific activation of tyramide using 3% hydrogen peroxide solution (Sigma-Aldrich) in PBS for 1 h. Subsequently, DNA was denaturated using freshly prepared 0.07 M NaOH in PBS for 5 min at room temperature, after which samples were incubated with PBS containing 0.5% BSA and 0.15% glycine for 1 h. The Click-it reaction was performed using 100 μl of the Click it reaction cocktail containing Azide-PEG3-Biotin Conjugate (20 μM in DMSO, Jena Bioscience), 1× Click-it reaction buffer (ThermoFisher Scientific), copper(III) sulfate (0.1 M) and 10× reaction buffer additive (ThermoFisher Scientific). .. Subsequently, for tyramide-based amplification, samples were incubated with 100 μl HRP–streptavidin conjugate (500 μg/ml) in 1% BSA for 1 h. Subsequently, samples were incubated for 10 min at room temperature with 100 μl amplification buffer containing 0.0015% H2 O2 and 1:100 dilution of Alexa-Fluor 488 nm labeled tyramide, which was dissolved in 150 μl DMSO (ThermoFisher Scientific).

    Protein Concentration:

    Article Title: An Activity-Based Probe for N-Acylethanolamine Acid Amidase
    Article Snippet: Briefly, the following reagents were added to the protein preparations at the indicated final concentration: 100μM Azide-PEG3-biotin conjugate (CLK-AZ104P4, Jena Bioscience), 1mM TCEP, 100 μM Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA), 1mM CuSO4.5H2O. .. The volumes of reaction were 2× 500μl (1mg/ml protein concentration) when streptavidin enrichment was required, and 50μl (1mg/ml protein concentration) when only protein blot analysis was performed.

    Concentration Assay:

    Article Title: An Activity-Based Probe for N-Acylethanolamine Acid Amidase
    Article Snippet: .. Briefly, the following reagents were added to the protein preparations at the indicated final concentration: 100μM Azide-PEG3-biotin conjugate (CLK-AZ104P4, Jena Bioscience), 1mM TCEP, 100 μM Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA), 1mM CuSO4.5H2O. ..

    Western Blot:

    Article Title: The oncoprotein DEK affects the outcome of PARP1/2 inhibition during mild replication stress
    Article Snippet: Click reaction to label EdU-containing DNA was performed using biotin-PEG3-azide (Jena Bioscience) for 90 min and cells were sonicated in a Bioruptor sonicator (Diogenode) to solubilize chromatin fragments. .. Chromatin bound proteins (“Capture”) were subjected to Western blot analysis using the following antibodies: polyclonal rabbit α-DEK K-877 (1:20,000; [ ]), monoclonal mouse α-PCNA (1:9,000; PC10, Cell Signaling Technology), polyclonal rabbit α-H3 (1:150,000; ab1791, Abcam).

    Activated Clotting Time Assay:

    Article Title: Biotinylation of Deoxyguanosine at the Abasic Site in Double-Stranded Oligodeoxynucleotides
    Article Snippet: As a control sample, the double-stranded DNA oligonucleotides DS-G/C without an abasic site was prepared by the PCR amplification with a set of primers, FW primer, 5′-(CAG TGA AGT TGG CAG ACT GAG C)-3′, and Rev primer, 5′-(CTG ACT TGC CAC CTA TAG ACA GC)-3′, and the single-stranded DNA oligonucleotides SS-G as the template. .. A mixture of 30 μ L (0.1 M CuBr/0.1 M TBTA 1 : 2 in DMSO/t-BuOH 3 : 1) and 20 μ L of Biotin-PEG3 -Azide solution (Jena Bioscience, USA) was added to 50 μ L of the solution of DNA in Tris buffer.

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  • 85
    Jena Bioscience azide peg3 biotin conjugate
    Azide Peg3 Biotin Conjugate, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/azide peg3 biotin conjugate/product/Jena Bioscience
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    azide peg3 biotin conjugate - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

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