azide peg3 biotin conjugate  (Jena Bioscience)


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    Name:
    Azide-PEG3-Biotin Conjugate
    Description:
    Azide-PEG3-Biotin is suitable for the introduction of a biotin moiety to terminal Alkyne- and strained Alkyne (e.g. DBCO)-labeled biomolecules via Cu(I)-catalyzed Alknye-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively. The hydrophilic PEG3 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose. Due to the extremely high affinity of biotin towards streptavidin (KD = 10-15 M), the biotinylated molecule/streptavidin-interaction is essentially irreversible under physiological conditions.
    Catalog Number:
    CLK-AZ104P4-100
    Molecular Weight:
    444.54 g/mol
    Price:
    293.66
    Purity:
    > 95 % (H NMR)
    Category:
    Click Chemistry
    Score:
    85
    Format:
    off-white to grey solid
    Formula:
    C18H32N6O5S
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    Structured Review

    Jena Bioscience azide peg3 biotin conjugate
    Azide-PEG3-Biotin is suitable for the introduction of a biotin moiety to terminal Alkyne- and strained Alkyne (e.g. DBCO)-labeled biomolecules via Cu(I)-catalyzed Alknye-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively. The hydrophilic PEG3 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose. Due to the extremely high affinity of biotin towards streptavidin (KD = 10-15 M), the biotinylated molecule/streptavidin-interaction is essentially irreversible under physiological conditions.
    https://www.bioz.com/result/azide peg3 biotin conjugate/product/Jena Bioscience
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    azide peg3 biotin conjugate - by Bioz Stars, 2019-12
    91/100 stars

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    Related Articles

    UDS Assay:

    Article Title: Amplification of unscheduled DNA synthesis signal enables fluorescence-based single cell quantification of transcription-coupled nucleotide excision repair
    Article Snippet: Paragraph title: Amplified UDS assay ... After blocking, endogenous peroxidases were quenched to reduce non-specific activation of tyramide using 3% hydrogen peroxide solution (Sigma-Aldrich) in PBS for 1 h. Subsequently, DNA was denaturated using freshly prepared 0.07 M NaOH in PBS for 5 min at room temperature, after which samples were incubated with PBS containing 0.5% BSA and 0.15% glycine for 1 h. The Click-it reaction was performed using 100 μl of the Click it reaction cocktail containing Azide-PEG3-Biotin Conjugate (20 μM in DMSO, Jena Bioscience), 1× Click-it reaction buffer (ThermoFisher Scientific), copper(III) sulfate (0.1 M) and 10× reaction buffer additive (ThermoFisher Scientific).

    Amplification:

    Article Title: Amplification of unscheduled DNA synthesis signal enables fluorescence-based single cell quantification of transcription-coupled nucleotide excision repair
    Article Snippet: Paragraph title: Amplified UDS assay ... After blocking, endogenous peroxidases were quenched to reduce non-specific activation of tyramide using 3% hydrogen peroxide solution (Sigma-Aldrich) in PBS for 1 h. Subsequently, DNA was denaturated using freshly prepared 0.07 M NaOH in PBS for 5 min at room temperature, after which samples were incubated with PBS containing 0.5% BSA and 0.15% glycine for 1 h. The Click-it reaction was performed using 100 μl of the Click it reaction cocktail containing Azide-PEG3-Biotin Conjugate (20 μM in DMSO, Jena Bioscience), 1× Click-it reaction buffer (ThermoFisher Scientific), copper(III) sulfate (0.1 M) and 10× reaction buffer additive (ThermoFisher Scientific).

    Concentration Assay:

    Article Title: An Activity-Based Probe for N-Acylethanolamine Acid Amidase
    Article Snippet: The click chemistry and the streptavidin enrichment were performed according to the protocol described in Speers A. E. et al. 2009. .. Briefly, the following reagents were added to the protein preparations at the indicated final concentration: 100μM Azide-PEG3-biotin conjugate (CLK-AZ104P4, Jena Bioscience), 1mM TCEP, 100 μM Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA), 1mM CuSO4.5H2O. .. TBTA was first dissolved in DMSO at 83.5mM and then diluted with four volumes of t-butanol.

    Blocking Assay:

    Article Title: Amplification of unscheduled DNA synthesis signal enables fluorescence-based single cell quantification of transcription-coupled nucleotide excision repair
    Article Snippet: After washing the cells twice in 3% BSA in PBS and twice in PBS, cells were mounted using Vectashield (Vector Laboratories) with DAPI to stain DNA and cover slips were sealed using nail polish. .. After blocking, endogenous peroxidases were quenched to reduce non-specific activation of tyramide using 3% hydrogen peroxide solution (Sigma-Aldrich) in PBS for 1 h. Subsequently, DNA was denaturated using freshly prepared 0.07 M NaOH in PBS for 5 min at room temperature, after which samples were incubated with PBS containing 0.5% BSA and 0.15% glycine for 1 h. The Click-it reaction was performed using 100 μl of the Click it reaction cocktail containing Azide-PEG3-Biotin Conjugate (20 μM in DMSO, Jena Bioscience), 1× Click-it reaction buffer (ThermoFisher Scientific), copper(III) sulfate (0.1 M) and 10× reaction buffer additive (ThermoFisher Scientific). .. Subsequently, for tyramide-based amplification, samples were incubated with 100 μl HRP–streptavidin conjugate (500 μg/ml) in 1% BSA for 1 h. Subsequently, samples were incubated for 10 min at room temperature with 100 μl amplification buffer containing 0.0015% H2 O2 and 1:100 dilution of Alexa-Fluor 488 nm labeled tyramide, which was dissolved in 150 μl DMSO (ThermoFisher Scientific).

    Activation Assay:

    Article Title: Amplification of unscheduled DNA synthesis signal enables fluorescence-based single cell quantification of transcription-coupled nucleotide excision repair
    Article Snippet: After washing the cells twice in 3% BSA in PBS and twice in PBS, cells were mounted using Vectashield (Vector Laboratories) with DAPI to stain DNA and cover slips were sealed using nail polish. .. After blocking, endogenous peroxidases were quenched to reduce non-specific activation of tyramide using 3% hydrogen peroxide solution (Sigma-Aldrich) in PBS for 1 h. Subsequently, DNA was denaturated using freshly prepared 0.07 M NaOH in PBS for 5 min at room temperature, after which samples were incubated with PBS containing 0.5% BSA and 0.15% glycine for 1 h. The Click-it reaction was performed using 100 μl of the Click it reaction cocktail containing Azide-PEG3-Biotin Conjugate (20 μM in DMSO, Jena Bioscience), 1× Click-it reaction buffer (ThermoFisher Scientific), copper(III) sulfate (0.1 M) and 10× reaction buffer additive (ThermoFisher Scientific). .. Subsequently, for tyramide-based amplification, samples were incubated with 100 μl HRP–streptavidin conjugate (500 μg/ml) in 1% BSA for 1 h. Subsequently, samples were incubated for 10 min at room temperature with 100 μl amplification buffer containing 0.0015% H2 O2 and 1:100 dilution of Alexa-Fluor 488 nm labeled tyramide, which was dissolved in 150 μl DMSO (ThermoFisher Scientific).

    Incubation:

    Article Title: Amplification of unscheduled DNA synthesis signal enables fluorescence-based single cell quantification of transcription-coupled nucleotide excision repair
    Article Snippet: After washing the cells twice in 3% BSA in PBS and twice in PBS, cells were mounted using Vectashield (Vector Laboratories) with DAPI to stain DNA and cover slips were sealed using nail polish. .. After blocking, endogenous peroxidases were quenched to reduce non-specific activation of tyramide using 3% hydrogen peroxide solution (Sigma-Aldrich) in PBS for 1 h. Subsequently, DNA was denaturated using freshly prepared 0.07 M NaOH in PBS for 5 min at room temperature, after which samples were incubated with PBS containing 0.5% BSA and 0.15% glycine for 1 h. The Click-it reaction was performed using 100 μl of the Click it reaction cocktail containing Azide-PEG3-Biotin Conjugate (20 μM in DMSO, Jena Bioscience), 1× Click-it reaction buffer (ThermoFisher Scientific), copper(III) sulfate (0.1 M) and 10× reaction buffer additive (ThermoFisher Scientific). .. Subsequently, for tyramide-based amplification, samples were incubated with 100 μl HRP–streptavidin conjugate (500 μg/ml) in 1% BSA for 1 h. Subsequently, samples were incubated for 10 min at room temperature with 100 μl amplification buffer containing 0.0015% H2 O2 and 1:100 dilution of Alexa-Fluor 488 nm labeled tyramide, which was dissolved in 150 μl DMSO (ThermoFisher Scientific).

    Protein Concentration:

    Article Title: An Activity-Based Probe for N-Acylethanolamine Acid Amidase
    Article Snippet: Briefly, the following reagents were added to the protein preparations at the indicated final concentration: 100μM Azide-PEG3-biotin conjugate (CLK-AZ104P4, Jena Bioscience), 1mM TCEP, 100 μM Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA), 1mM CuSO4.5H2O. .. Briefly, the following reagents were added to the protein preparations at the indicated final concentration: 100μM Azide-PEG3-biotin conjugate (CLK-AZ104P4, Jena Bioscience), 1mM TCEP, 100 μM Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA), 1mM CuSO4.5H2O.

    Sonication:

    Article Title: An Activity-Based Probe for N-Acylethanolamine Acid Amidase
    Article Snippet: Briefly, the following reagents were added to the protein preparations at the indicated final concentration: 100μM Azide-PEG3-biotin conjugate (CLK-AZ104P4, Jena Bioscience), 1mM TCEP, 100 μM Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA), 1mM CuSO4.5H2O. .. After the reaction time the samples were either directly analyzed by protein blot or streptavidin enriched as described.

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  • 91
    Jena Bioscience azide peg3 biotin conjugate
    Azide Peg3 Biotin Conjugate, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/azide peg3 biotin conjugate/product/Jena Bioscience
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    azide peg3 biotin conjugate - by Bioz Stars, 2019-12
    91/100 stars
      Buy from Supplier

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