azide peg3 biotin conjugate  (Jena Bioscience)


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Bioz Manufacturer Symbol Jena Bioscience manufactures this product  
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    Name:
    Azide PEG3 Biotin Conjugate
    Description:
    Azide PEG3 Biotin is suitable for the introduction of a biotin moiety to terminal Alkyne and strained Alkyne e g DBCO labeled biomolecules via Cu I catalyzed Alknye Azide CUAAC or Cu I free strain promoted Alkyne Azide Click Chemistry SPAAC reaction respectively The hydrophilic PEG3 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP labeled streptavidin or its affinity purification via streptavidin agarose Due to the extremely high affinity of biotin towards streptavidin KD 10 15 M the biotinylated molecule streptavidin interaction is essentially irreversible under physiological conditions
    Catalog Number:
    clk-az104p4-100
    Molecular Weight:
    444.54 g/mol
    Price:
    293.66
    Purity:
    > 95 % (H NMR)
    Category:
    Click Chemistry
    Format:
    off-white to grey solid
    Formula:
    C18H32N6O5S
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    Structured Review

    Jena Bioscience azide peg3 biotin conjugate
    Azide PEG3 Biotin is suitable for the introduction of a biotin moiety to terminal Alkyne and strained Alkyne e g DBCO labeled biomolecules via Cu I catalyzed Alknye Azide CUAAC or Cu I free strain promoted Alkyne Azide Click Chemistry SPAAC reaction respectively The hydrophilic PEG3 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP labeled streptavidin or its affinity purification via streptavidin agarose Due to the extremely high affinity of biotin towards streptavidin KD 10 15 M the biotinylated molecule streptavidin interaction is essentially irreversible under physiological conditions
    https://www.bioz.com/result/azide peg3 biotin conjugate/product/Jena Bioscience
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    azide peg3 biotin conjugate - by Bioz Stars, 2020-09
    95/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: NAD captureSeq indicates NAD as a bacterial cap for a subset of regulatory RNAs.
    Article Snippet: .. A distinctive feature of prokaryotic gene expression is the absence of 5'-capped RNA. .. A distinctive feature of prokaryotic gene expression is the absence of 5'-capped RNA.

    Isolation:

    Article Title: NAD captureSeq indicates NAD as a bacterial cap for a subset of regulatory RNAs.
    Article Snippet: .. A distinctive feature of prokaryotic gene expression is the absence of 5'-capped RNA. .. A distinctive feature of prokaryotic gene expression is the absence of 5'-capped RNA.

    Concentration Assay:

    Article Title: An Activity-Based Probe for N-Acylethanolamine Acid Amidase
    Article Snippet: .. Briefly, the following reagents were added to the protein preparations at the indicated final concentration: 100μM Azide-PEG3-biotin conjugate (CLK-AZ104P4, Jena Bioscience), 1mM TCEP, 100 μM Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA), 1mM CuSO4.5H2O. ..

    Incubation:

    Article Title: NAD captureSeq indicates NAD as a bacterial cap for a subset of regulatory RNAs.
    Article Snippet: .. A distinctive feature of prokaryotic gene expression is the absence of 5'-capped RNA. .. A distinctive feature of prokaryotic gene expression is the absence of 5'-capped RNA.

    Sonication:

    Article Title: The oncoprotein DEK affects the outcome of PARP1/2 inhibition during mild replication stress
    Article Snippet: .. Click reaction to label EdU-containing DNA was performed using biotin-PEG3-azide (Jena Bioscience) for 90 min and cells were sonicated in a Bioruptor sonicator (Diogenode) to solubilize chromatin fragments. .. Biotin-linked fragments were precipitated overnight at 4°C using streptavidin-coupled magnetic beads (0.8 μm, Solulink).

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  • 95
    Jena Bioscience azide peg3 biotin conjugate
    Azide Peg3 Biotin Conjugate, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/azide peg3 biotin conjugate/product/Jena Bioscience
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    azide peg3 biotin conjugate - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

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