Structured Review

Axygen axypreptm pcr cleanup kit
Axypreptm Pcr Cleanup Kit, supplied by Axygen, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axypreptm pcr cleanup kit/product/Axygen
Average 88 stars, based on 8 article reviews
Price from $9.99 to $1999.99
axypreptm pcr cleanup kit - by Bioz Stars, 2020-09
88/100 stars

Images

Related Articles

Amplification:

Article Title: Klebsiella Phage KP34 RNA Polymerase and Its Use in RNA Synthesis
Article Snippet: .. PCR products were purified by AxyPrepTM PCR Cleanup Kit, and then ligated to T-Vector pMD19 with T4 DNA ligase (2X ligation Mix); the reaction was incubated at 16°C for 30 min. Plasmids containing inserted sequences were amplified and sent for sequencing. ..

Ligation:

Article Title: Klebsiella Phage KP34 RNA Polymerase and Its Use in RNA Synthesis
Article Snippet: .. PCR products were purified by AxyPrepTM PCR Cleanup Kit, and then ligated to T-Vector pMD19 with T4 DNA ligase (2X ligation Mix); the reaction was incubated at 16°C for 30 min. Plasmids containing inserted sequences were amplified and sent for sequencing. ..

Purification:

Article Title: Characterization of the Copy Number and Variants of Deformed Wing Virus (DWV) in the Pairs of Honey Bee Pupa and Infesting Varroa destructor or Tropilaelaps mercedesae
Article Snippet: .. Target DNA was prepared by PCR, followed by purification with a AxyPrepTM PCR cleanup kit (Axygen). .. DNA concentration was measured using a Nanodrop 2000 spectrophotometer (Thermo Fischer) to calculate copy number using the formula Copy number = DNA concentration (ng/μL) × 6.02 × 1023 (copies/mol)/length (bp) × 6.6 × 1011 (ng/mol), in which 6.6 × 1011 ng/mol is the average molecular mass of one base pair and 6.022 × 1023 copies/mol is Avogadro’s number.

Article Title: Toxoplasma gondii prevalence in food animals and rodents in different regions of China: isolation, genotyping and mouse pathogenicity
Article Snippet: .. The remaining products were purified using AxyPrepTM PCR Cleanup Kit (Axygen, Union, USA) and digested with appropriate restriction endonucleases [ ]. .. The restriction fragments were run by electrophoresis through a 2.5% ethidium bromide stained agarose gel for all markers except Apico, which required a 3% gel, and then visualized under UV light.

Article Title: A Novel Partitivirus in the Hypovirulent Isolate QT5-19 of the Plant Pathogenic Fungus Botrytis cinerea
Article Snippet: .. The pure dsRNAs were ligated with the adaptor 110a ( ) at the 3′-terminus using T4 RNA ligase (Promega, Madison, WI, USA) at 16 °C for 12 h. The adaptor-ligated dsRNAs were purified using AxyPrepTM PCR cleanup kit (Axygen Scientific Inc., Union City, CA, USA). ..

Article Title: Klebsiella Phage KP34 RNA Polymerase and Its Use in RNA Synthesis
Article Snippet: .. PCR products and linearized plasmids were purified using AxyPrepTM PCR Cleanup Kit (Axygen), and DNA concentrations were determined by NanoPhotometer® (Implen). .. Transcription Assays For the assays shown in , , , , the transcription reaction mixtures (10 μl) contained 40 mM Tris–HCl (pH 8.0), 2 mM spermidine, 20 mM DTT, 20 mM MgCl2, 4 mM of each of the 4 NTPs (with 0 to 4 of the NTPs replaced by their dNTP or 2′-F-dNTP analogs), 40 U/μl RNaseOUTTM Recombinant Ribonuclease Inhibitor, 0.04 U/μl E. coli inorganic pyrophosphatase, DNA template (2 μM annealed oligonucleotides, 20 nM linearized plasmids, or 0.5 μM PCR products), and RNA polymerase (1 μM KP34 RNAP or its mutants, or 0.2 μM T7 RNAP or 1 μM Syn5 RNAP).

Article Title: Klebsiella Phage KP34 RNA Polymerase and Its Use in RNA Synthesis
Article Snippet: .. PCR products were purified by AxyPrepTM PCR Cleanup Kit, and then ligated to T-Vector pMD19 with T4 DNA ligase (2X ligation Mix); the reaction was incubated at 16°C for 30 min. Plasmids containing inserted sequences were amplified and sent for sequencing. ..

Polymerase Chain Reaction:

Article Title: Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite
Article Snippet: .. The AxyPrepTM DNA gel extraction kit, AxyPrepTM plasmid miniprep kit, and AxyPrepTM PCR cleanup kit were obtained from Axygen (USA). .. A fosmid library was constructed with the vector of pCC2FOS™ (Epicentre, USA) and the host strain of EPI300™-T1R (Epicentre, USA).

Article Title: Characterization of the Copy Number and Variants of Deformed Wing Virus (DWV) in the Pairs of Honey Bee Pupa and Infesting Varroa destructor or Tropilaelaps mercedesae
Article Snippet: .. Target DNA was prepared by PCR, followed by purification with a AxyPrepTM PCR cleanup kit (Axygen). .. DNA concentration was measured using a Nanodrop 2000 spectrophotometer (Thermo Fischer) to calculate copy number using the formula Copy number = DNA concentration (ng/μL) × 6.02 × 1023 (copies/mol)/length (bp) × 6.6 × 1011 (ng/mol), in which 6.6 × 1011 ng/mol is the average molecular mass of one base pair and 6.022 × 1023 copies/mol is Avogadro’s number.

Article Title: Toxoplasma gondii prevalence in food animals and rodents in different regions of China: isolation, genotyping and mouse pathogenicity
Article Snippet: .. The remaining products were purified using AxyPrepTM PCR Cleanup Kit (Axygen, Union, USA) and digested with appropriate restriction endonucleases [ ]. .. The restriction fragments were run by electrophoresis through a 2.5% ethidium bromide stained agarose gel for all markers except Apico, which required a 3% gel, and then visualized under UV light.

Article Title: A Novel Partitivirus in the Hypovirulent Isolate QT5-19 of the Plant Pathogenic Fungus Botrytis cinerea
Article Snippet: .. The pure dsRNAs were ligated with the adaptor 110a ( ) at the 3′-terminus using T4 RNA ligase (Promega, Madison, WI, USA) at 16 °C for 12 h. The adaptor-ligated dsRNAs were purified using AxyPrepTM PCR cleanup kit (Axygen Scientific Inc., Union City, CA, USA). ..

Article Title: Klebsiella Phage KP34 RNA Polymerase and Its Use in RNA Synthesis
Article Snippet: .. PCR products and linearized plasmids were purified using AxyPrepTM PCR Cleanup Kit (Axygen), and DNA concentrations were determined by NanoPhotometer® (Implen). .. Transcription Assays For the assays shown in , , , , the transcription reaction mixtures (10 μl) contained 40 mM Tris–HCl (pH 8.0), 2 mM spermidine, 20 mM DTT, 20 mM MgCl2, 4 mM of each of the 4 NTPs (with 0 to 4 of the NTPs replaced by their dNTP or 2′-F-dNTP analogs), 40 U/μl RNaseOUTTM Recombinant Ribonuclease Inhibitor, 0.04 U/μl E. coli inorganic pyrophosphatase, DNA template (2 μM annealed oligonucleotides, 20 nM linearized plasmids, or 0.5 μM PCR products), and RNA polymerase (1 μM KP34 RNAP or its mutants, or 0.2 μM T7 RNAP or 1 μM Syn5 RNAP).

Article Title: Klebsiella Phage KP34 RNA Polymerase and Its Use in RNA Synthesis
Article Snippet: .. PCR products were purified by AxyPrepTM PCR Cleanup Kit, and then ligated to T-Vector pMD19 with T4 DNA ligase (2X ligation Mix); the reaction was incubated at 16°C for 30 min. Plasmids containing inserted sequences were amplified and sent for sequencing. ..

Incubation:

Article Title: Klebsiella Phage KP34 RNA Polymerase and Its Use in RNA Synthesis
Article Snippet: .. PCR products were purified by AxyPrepTM PCR Cleanup Kit, and then ligated to T-Vector pMD19 with T4 DNA ligase (2X ligation Mix); the reaction was incubated at 16°C for 30 min. Plasmids containing inserted sequences were amplified and sent for sequencing. ..

Sequencing:

Article Title: Klebsiella Phage KP34 RNA Polymerase and Its Use in RNA Synthesis
Article Snippet: .. PCR products were purified by AxyPrepTM PCR Cleanup Kit, and then ligated to T-Vector pMD19 with T4 DNA ligase (2X ligation Mix); the reaction was incubated at 16°C for 30 min. Plasmids containing inserted sequences were amplified and sent for sequencing. ..

Gel Extraction:

Article Title: Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite
Article Snippet: .. The AxyPrepTM DNA gel extraction kit, AxyPrepTM plasmid miniprep kit, and AxyPrepTM PCR cleanup kit were obtained from Axygen (USA). .. A fosmid library was constructed with the vector of pCC2FOS™ (Epicentre, USA) and the host strain of EPI300™-T1R (Epicentre, USA).

Plasmid Preparation:

Article Title: Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite
Article Snippet: .. The AxyPrepTM DNA gel extraction kit, AxyPrepTM plasmid miniprep kit, and AxyPrepTM PCR cleanup kit were obtained from Axygen (USA). .. A fosmid library was constructed with the vector of pCC2FOS™ (Epicentre, USA) and the host strain of EPI300™-T1R (Epicentre, USA).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89
    Axygen axyprep pcr cleanup kit
    Axyprep Pcr Cleanup Kit, supplied by Axygen, used in various techniques. Bioz Stars score: 89/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axyprep pcr cleanup kit/product/Axygen
    Average 89 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    axyprep pcr cleanup kit - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    Image Search Results