Structured Review

Axygen axyprep mag pcr clean up
Axyprep Mag Pcr Clean Up, supplied by Axygen, used in various techniques. Bioz Stars score: 90/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axyprep mag pcr clean up/product/Axygen
Average 90 stars, based on 48 article reviews
Price from $9.99 to $1999.99
axyprep mag pcr clean up - by Bioz Stars, 2020-08
90/100 stars

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Polymerase Chain Reaction:

Article Title: Regulation of zebrafish dorsoventral patterning by phase separation of RNA-binding protein Rbm14
Article Snippet: .. The PCR products were cleaned up using AxyPrep Mag PCR Clean-up (Axygen), validated using an Agilent 2100 Bioanalyzer (Agilent Technologies), and quantified by a Qubit 2.0 Fluorometer (Invitrogen). .. Then the libraries with different indices were multiplexed and loaded on an Illumina HiSeq instrument according to the manufacturer’s instructions (Illumina).

Article Title: CD24 regulates sorafenib resistance via activating autophagy in hepatocellular carcinoma
Article Snippet: .. In brief, the PCR products were cleaned up using AxyPrep Mag PCR Clean-up (Axygen), validated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA). .. Then libraries with different indices were multiplexed and loaded on an Illumina HiSeq instrument according to manufacturer’s instructions (Illumina, San Diego, CA, USA).

Article Title: A human ciliopathy with polycystic ovarian syndrome and multiple subcutaneous cysts
Article Snippet: .. Size selection of adaptor-ligated DNA was then performed using AxyPrep Mag PCR Clean-up (Axygen), and fragments of ∼410 bp (with an approximate insert size of 350 bp) were recovered. .. Each sample was then amplified by PCR for 8 cycles using P5 and P7 primers, with both primers carrying sequences that can anneal with the flowcell to perform bridge PCR and the P7 primer carrying a six-base index allowing for multiplexing.

Article Title: Global transcriptomic study of circRNAs expression profile in sorafenib resistant hepatocellular carcinoma cells
Article Snippet: .. Size selection of Adaptor-ligated DNA was then performed using AxyPrep Mag PCR Clean-up (Axygen), and fragments of ~360 bp (with the approximate insert size of 300 bp) were recovered. .. The dUTP-marked second strand was digested with Uracil-Specific Excision Reagent (USER) enzyme (New England Biolabs).

Article Title: Transcriptomic Profiling of 3D Glioblastoma Tumoroids for the Identification of Mechanisms Involved in Anticancer Drug Resistance
Article Snippet: .. The PCR products were cleaned up using AxyPrep Mag PCR Clean-up (Axygen), validated using an Agilent 2100 Bioanalyzer (Agilent Technologies), and quantified by Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA). .. Libraries with different indices were multiplexed and loaded on an Illumina HiSeq instrument according to manufacturer’s instructions (Illumina).

Article Title: Zfp422 promotes skeletal muscle differentiation by regulating EphA7 to induce appropriate myoblast apoptosis
Article Snippet: .. Adapter-ligated DNA was then recovered using AxyPrep Mag PCR Clean-up (Axygen). .. Each sample was then amplified by PCR for eight cycles using P5 and P7 primers, with both primers carrying sequences which can anneal with Flowcell to perform bridge PCR and P7 primer carrying a six-base index allowing for multiplexing.

Article Title: Global transcriptomic study of circRNAs expression profile in sorafenib resistant hepatocellular carcinoma cells
Article Snippet: .. The purified double-stranded cDNA by AxyPrep Mag PCR Clean-up (Axygen) was then treated with End Prep Enzyme Mix to repair both ends and add a dA-tailing in one reaction, followed by a T-A ligation to add adaptors to both ends. .. Size selection of Adaptor-ligated DNA was then performed using AxyPrep Mag PCR Clean-up (Axygen), and fragments of ~360 bp (with the approximate insert size of 300 bp) were recovered.

Purification:

Article Title: Global transcriptomic study of circRNAs expression profile in sorafenib resistant hepatocellular carcinoma cells
Article Snippet: .. The purified double-stranded cDNA by AxyPrep Mag PCR Clean-up (Axygen) was then treated with End Prep Enzyme Mix to repair both ends and add a dA-tailing in one reaction, followed by a T-A ligation to add adaptors to both ends. .. Size selection of Adaptor-ligated DNA was then performed using AxyPrep Mag PCR Clean-up (Axygen), and fragments of ~360 bp (with the approximate insert size of 300 bp) were recovered.

Selection:

Article Title: A human ciliopathy with polycystic ovarian syndrome and multiple subcutaneous cysts
Article Snippet: .. Size selection of adaptor-ligated DNA was then performed using AxyPrep Mag PCR Clean-up (Axygen), and fragments of ∼410 bp (with an approximate insert size of 350 bp) were recovered. .. Each sample was then amplified by PCR for 8 cycles using P5 and P7 primers, with both primers carrying sequences that can anneal with the flowcell to perform bridge PCR and the P7 primer carrying a six-base index allowing for multiplexing.

Article Title: Global transcriptomic study of circRNAs expression profile in sorafenib resistant hepatocellular carcinoma cells
Article Snippet: .. Size selection of Adaptor-ligated DNA was then performed using AxyPrep Mag PCR Clean-up (Axygen), and fragments of ~360 bp (with the approximate insert size of 300 bp) were recovered. .. The dUTP-marked second strand was digested with Uracil-Specific Excision Reagent (USER) enzyme (New England Biolabs).

Ligation:

Article Title: Global transcriptomic study of circRNAs expression profile in sorafenib resistant hepatocellular carcinoma cells
Article Snippet: .. The purified double-stranded cDNA by AxyPrep Mag PCR Clean-up (Axygen) was then treated with End Prep Enzyme Mix to repair both ends and add a dA-tailing in one reaction, followed by a T-A ligation to add adaptors to both ends. .. Size selection of Adaptor-ligated DNA was then performed using AxyPrep Mag PCR Clean-up (Axygen), and fragments of ~360 bp (with the approximate insert size of 300 bp) were recovered.

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  • 89
    Axygen axyprep mag pcr clean up kit
    Axyprep Mag Pcr Clean Up Kit, supplied by Axygen, used in various techniques. Bioz Stars score: 89/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axyprep mag pcr clean up kit/product/Axygen
    Average 89 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    axyprep mag pcr clean up kit - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    90
    Axygen axyprep mag pcr clean up
    Axyprep Mag Pcr Clean Up, supplied by Axygen, used in various techniques. Bioz Stars score: 90/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axyprep mag pcr clean up/product/Axygen
    Average 90 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    axyprep mag pcr clean up - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    90
    Axygen axyprep mag pcr clean up beads
    Axyprep Mag Pcr Clean Up Beads, supplied by Axygen, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axyprep mag pcr clean up beads/product/Axygen
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    axyprep mag pcr clean up beads - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

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