Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Identification of a Transmembrane Protein Involved in Shear Stress Signaling and Hepatocarcinogenesis After a Sustained Virological Response to Hepatitis C Virus

doi: 10.1016/j.jcmgh.2023.04.006

Figure Lengend Snippet: Expression of TMEM164 and ER stress signals in a perfusion culture. ( A ) Illustration of the perfusion culture apparatus. Alvetex Perfusion Plate and Alvetex Scaffold 6-well inserts were used ( http://resources.amsbio.com/Application-Note/Alvetex-Perfusion-Plate.pdf was modified). ( B ) TMEM164 , IL6 , ATF6 , ERN1 , and EIF2AK3 expression in cells treated with TMEM164 siRNA in a perfusion culture. TMEM164 expression was increased after 12-hour perfusion. IL6 , ATF6 , ERN1 , and EIF2AK3 expression was also increased with TMEM164 expression. TMEM164 siRNA efficiently suppressed TMEM164 expression, resulting in marked decreases of IL6 and ATF6 expression but not of ERN1 and EIF2AK3 expression. ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001, ∗∗∗∗ P < .0001, vs control subjects. ( C ) TMEM164 , IL6 , and ATF6 expression in cells treated with ATF6 siRNA in a perfusion culture. Suppression of ATF6 expression resulted in a decrease of IL6 and TMEM164 levels. ∗∗ P < .01, ∗∗∗ P < .001, ∗∗∗∗ P < .0001, vs control subjects.

Article Snippet: After 90-minute culture, Alvetex Scaffold 6-well inserts were placed in an Alvetex Perfusion Plate (Reprocell, Telangana, India), and 9 mL culture solution was added.

Techniques: Expressing, Modification