Structured Review

Novocastra avidin biotin peroxidase complex
Avidin Biotin Peroxidase Complex, supplied by Novocastra, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/avidin biotin peroxidase complex/product/Novocastra
Average 93 stars, based on 14 article reviews
Price from $9.99 to $1999.99
avidin biotin peroxidase complex - by Bioz Stars, 2020-09
93/100 stars

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Transduction:

Article Title: Tissue Microarray Analysis of Fas and FasL Expressions in Human Non-small Cell Lung Carcinomas; with Reference to the p53 and bcl-2 Overexpressions
Article Snippet: .. Immunohistochemical staining and its semiquantitative analysis The standard avidin-biotin-peroxidase complex method was used for the immunohistochemical examination with mouse monoclonal antibodies against Fas (GM30, Novocastra, Newcastle upon Tyne, U.K.), FasL (clone 33, Transduction Lab., Lexington, KY, U.S.A.), p53 (DO7, Novocastra), and bcl-2 (clone 124, DAKO, Goldstrup, Denmark). ..

Staining:

Article Title: Prognostic Significance of Immunohistochemical Expression of p53 and Retinoblastoma Gene Protein (pRB) in Curatively Resected Gastric Cancer
Article Snippet: .. Immunohistochemical staining Immunohistochemical staining was performed using the avidin-biotin-peroxidase complex with monoclonal antibodies against p 53 (NCL-p53-D07, Novocastra Laboratories, Newcastle, United Kingdom), and Rb (14001A, Pharmingen, USA). .. Representative paraffin blocks containing tumors isolated from each case subject were sectioned into 5 µm slices and affixed to slides, then dried for 1 hour at 60℃.

Article Title: Tissue Microarray Analysis of Fas and FasL Expressions in Human Non-small Cell Lung Carcinomas; with Reference to the p53 and bcl-2 Overexpressions
Article Snippet: .. Immunohistochemical staining and its semiquantitative analysis The standard avidin-biotin-peroxidase complex method was used for the immunohistochemical examination with mouse monoclonal antibodies against Fas (GM30, Novocastra, Newcastle upon Tyne, U.K.), FasL (clone 33, Transduction Lab., Lexington, KY, U.S.A.), p53 (DO7, Novocastra), and bcl-2 (clone 124, DAKO, Goldstrup, Denmark). ..

Immunohistochemistry:

Article Title: Orexin Neurons Contribute to Central Modulation of Respiratory Drive by Progestins on ex vivo Newborn Rodent Preparations
Article Snippet: .. Immunohistochemical detection of c-FOS was processed on free-floating coronal sections (40 μm) by incubation with first, a rabbit polyclonal antibody against c-FOS (sc-253; Santa Cruz Biotechnology Inc., CA, United States, 1:10000, in 1% bovine serum albumin, BSA; 48 h, 4°C), second a biotinylated goat anti-rabbit IgG antibody (Vector Laboratories, Burlington, Canada, 1:500; 2 h), and finally an avidin-biotin-peroxidase complex (ABC; Novostain Super ABC kit, Novocastra Laboratories, Newcastle, United Kingdom, 1:250; 1 h). .. Peroxidase activity was detected using a solution containing 0.02% 3,3′- diaminobenzidine tetrahydrochloride, 0.04% nickel ammonium sulfate and 0.01% H2 O2 in 0.05 M Tris–HCl buffer (pH 7.6), which results in a blue/gray chromogen.

Article Title: Prognostic Significance of Immunohistochemical Expression of p53 and Retinoblastoma Gene Protein (pRB) in Curatively Resected Gastric Cancer
Article Snippet: .. Immunohistochemical staining Immunohistochemical staining was performed using the avidin-biotin-peroxidase complex with monoclonal antibodies against p 53 (NCL-p53-D07, Novocastra Laboratories, Newcastle, United Kingdom), and Rb (14001A, Pharmingen, USA). .. Representative paraffin blocks containing tumors isolated from each case subject were sectioned into 5 µm slices and affixed to slides, then dried for 1 hour at 60℃.

Article Title: Tissue Microarray Analysis of Fas and FasL Expressions in Human Non-small Cell Lung Carcinomas; with Reference to the p53 and bcl-2 Overexpressions
Article Snippet: .. Immunohistochemical staining and its semiquantitative analysis The standard avidin-biotin-peroxidase complex method was used for the immunohistochemical examination with mouse monoclonal antibodies against Fas (GM30, Novocastra, Newcastle upon Tyne, U.K.), FasL (clone 33, Transduction Lab., Lexington, KY, U.S.A.), p53 (DO7, Novocastra), and bcl-2 (clone 124, DAKO, Goldstrup, Denmark). ..

Incubation:

Article Title: Orexin Neurons Contribute to Central Modulation of Respiratory Drive by Progestins on ex vivo Newborn Rodent Preparations
Article Snippet: .. Immunohistochemical detection of c-FOS was processed on free-floating coronal sections (40 μm) by incubation with first, a rabbit polyclonal antibody against c-FOS (sc-253; Santa Cruz Biotechnology Inc., CA, United States, 1:10000, in 1% bovine serum albumin, BSA; 48 h, 4°C), second a biotinylated goat anti-rabbit IgG antibody (Vector Laboratories, Burlington, Canada, 1:500; 2 h), and finally an avidin-biotin-peroxidase complex (ABC; Novostain Super ABC kit, Novocastra Laboratories, Newcastle, United Kingdom, 1:250; 1 h). .. Peroxidase activity was detected using a solution containing 0.02% 3,3′- diaminobenzidine tetrahydrochloride, 0.04% nickel ammonium sulfate and 0.01% H2 O2 in 0.05 M Tris–HCl buffer (pH 7.6), which results in a blue/gray chromogen.

Article Title: Alteration of the Cardiac Sympathetic Innervation Is Modulated by Duration of Diabetes in Female Rats
Article Snippet: .. The secondary antibody (incubation for 45 min at 37°C) and avidin-biotin peroxidase complex (45 min, 37°C) were applied, using the Novostain Super ABC Universal Kit (Novocastra Laboratories Ltd., Newcastle upon Tyne, UK). .. Following the immunohistochemistry, the background tissue was stained with Gill's haematoxylin (30 s; Bio-Optica, Milano, Italy).

Article Title: Suppression of Extravillous Trophoblast Vascular Endothelial Growth Factor Expression and Uterine Spiral Artery Invasion by Estrogen during Early Baboon Pregnancy
Article Snippet: .. Tissues were incubated 1 h (room temperature) with either biotinylated antimouse or antirabbit immunoglobulin (Novocastra/Vector) and 1 h with an avidin-biotin-peroxidase complex (ABC Elite; Novocastra/Vector). .. Tissue sections were developed using diaminobenzidine (Sigma, St. Louis, MO) and lightly counterstained with hematoxylin.

Article Title: BAG3 regulates formation of the SNARE complex and insulin secretion
Article Snippet: .. After washing thoroughly with PBS, sections were incubated using a biotinylated secondary anti-mouse IgG for 20 min, then rinsed, incubated using avidin-biotin-peroxidase complex (Novocastra; Leica Microsystems, Milan, Italy) and developed using diaminobenzidine (Sigma-Aldrich). .. Finally, the sections were counterstained using hematoxylin, dehydrated in alcohol, cleared in xylene and mounted using Permount (Fisher Scientific Inc., Milan, Italy).

Avidin-Biotin Assay:

Article Title: Orexin Neurons Contribute to Central Modulation of Respiratory Drive by Progestins on ex vivo Newborn Rodent Preparations
Article Snippet: .. Immunohistochemical detection of c-FOS was processed on free-floating coronal sections (40 μm) by incubation with first, a rabbit polyclonal antibody against c-FOS (sc-253; Santa Cruz Biotechnology Inc., CA, United States, 1:10000, in 1% bovine serum albumin, BSA; 48 h, 4°C), second a biotinylated goat anti-rabbit IgG antibody (Vector Laboratories, Burlington, Canada, 1:500; 2 h), and finally an avidin-biotin-peroxidase complex (ABC; Novostain Super ABC kit, Novocastra Laboratories, Newcastle, United Kingdom, 1:250; 1 h). .. Peroxidase activity was detected using a solution containing 0.02% 3,3′- diaminobenzidine tetrahydrochloride, 0.04% nickel ammonium sulfate and 0.01% H2 O2 in 0.05 M Tris–HCl buffer (pH 7.6), which results in a blue/gray chromogen.

Article Title: Syndecan-1 and E-cadherin expression in differentiated type of early gastric cancer
Article Snippet: .. The avidin-biotin peroxidase complex method was employed for the detection of human gastric mucin (HGM; Novocastra Laboratories, Newcastle, UK), MUC2 (Novocastra Laboratories), CD10 (Novocastra Laboratories). .. Paradoxical concanavalin A (Con A) staining, identifying class III mucins in mucous neck and pyloric gland cells, was employed, according to the method of Katsuyama and Spicer[ ].

Article Title: Prognostic Significance of Immunohistochemical Expression of p53 and Retinoblastoma Gene Protein (pRB) in Curatively Resected Gastric Cancer
Article Snippet: .. Immunohistochemical staining Immunohistochemical staining was performed using the avidin-biotin-peroxidase complex with monoclonal antibodies against p 53 (NCL-p53-D07, Novocastra Laboratories, Newcastle, United Kingdom), and Rb (14001A, Pharmingen, USA). .. Representative paraffin blocks containing tumors isolated from each case subject were sectioned into 5 µm slices and affixed to slides, then dried for 1 hour at 60℃.

Article Title: Alteration of the Cardiac Sympathetic Innervation Is Modulated by Duration of Diabetes in Female Rats
Article Snippet: .. The secondary antibody (incubation for 45 min at 37°C) and avidin-biotin peroxidase complex (45 min, 37°C) were applied, using the Novostain Super ABC Universal Kit (Novocastra Laboratories Ltd., Newcastle upon Tyne, UK). .. Following the immunohistochemistry, the background tissue was stained with Gill's haematoxylin (30 s; Bio-Optica, Milano, Italy).

Article Title: Suppression of Extravillous Trophoblast Vascular Endothelial Growth Factor Expression and Uterine Spiral Artery Invasion by Estrogen during Early Baboon Pregnancy
Article Snippet: .. Tissues were incubated 1 h (room temperature) with either biotinylated antimouse or antirabbit immunoglobulin (Novocastra/Vector) and 1 h with an avidin-biotin-peroxidase complex (ABC Elite; Novocastra/Vector). .. Tissue sections were developed using diaminobenzidine (Sigma, St. Louis, MO) and lightly counterstained with hematoxylin.

Article Title: BAG3 regulates formation of the SNARE complex and insulin secretion
Article Snippet: .. After washing thoroughly with PBS, sections were incubated using a biotinylated secondary anti-mouse IgG for 20 min, then rinsed, incubated using avidin-biotin-peroxidase complex (Novocastra; Leica Microsystems, Milan, Italy) and developed using diaminobenzidine (Sigma-Aldrich). .. Finally, the sections were counterstained using hematoxylin, dehydrated in alcohol, cleared in xylene and mounted using Permount (Fisher Scientific Inc., Milan, Italy).

Article Title: Tissue Microarray Analysis of Fas and FasL Expressions in Human Non-small Cell Lung Carcinomas; with Reference to the p53 and bcl-2 Overexpressions
Article Snippet: .. Immunohistochemical staining and its semiquantitative analysis The standard avidin-biotin-peroxidase complex method was used for the immunohistochemical examination with mouse monoclonal antibodies against Fas (GM30, Novocastra, Newcastle upon Tyne, U.K.), FasL (clone 33, Transduction Lab., Lexington, KY, U.S.A.), p53 (DO7, Novocastra), and bcl-2 (clone 124, DAKO, Goldstrup, Denmark). ..

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  • 93
    Novocastra avidin biotin peroxidase complex
    Avidin Biotin Peroxidase Complex, supplied by Novocastra, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin peroxidase complex/product/Novocastra
    Average 93 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    88
    Novocastra avidin biotin peroxidase complex kit reagents
    Avidin Biotin Peroxidase Complex Kit Reagents, supplied by Novocastra, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin peroxidase complex kit reagents/product/Novocastra
    Average 88 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex kit reagents - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    86
    Novocastra short polymer peroxidase complex
    Short Polymer Peroxidase Complex, supplied by Novocastra, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/short polymer peroxidase complex/product/Novocastra
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    short polymer peroxidase complex - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

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