avidin biotin peroxidase complex solution  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Name:
    VECTASTAIN ABC HRP Kit Peroxidase Standard
    Description:
    VECTASTAIN ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes Original VECTASTAIN ABC Kit Good sensitivity low backgroundOriginal avidin biotin ABC complex formulationLow costAvailable without Standard kit or with biotinylated species specific secondary antibody Kit Contents 2 ml Reagent A2 ml Reagent BReagents A and B form the ABC enzyme complex The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    Catalog Number:
    PK-4000
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    No antibody included
    Size:
    1 Kit
    Buy from Supplier


    Structured Review

    Vector Laboratories avidin biotin peroxidase complex solution
    VECTASTAIN ABC HRP Kit Peroxidase Standard
    VECTASTAIN ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes Original VECTASTAIN ABC Kit Good sensitivity low backgroundOriginal avidin biotin ABC complex formulationLow costAvailable without Standard kit or with biotinylated species specific secondary antibody Kit Contents 2 ml Reagent A2 ml Reagent BReagents A and B form the ABC enzyme complex The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    https://www.bioz.com/result/avidin biotin peroxidase complex solution/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex solution - by Bioz Stars, 2021-04
    98/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Progesterone Synthesized by Schwann Cells during Myelin Formation Regulates Neuronal Gene Expression
    Article Snippet: The coverslips were washed in phosphate-buffered saline and treated with a rat adsorbed biotinylated antimouse secondary antibody (Vector Laboratories, Burlingame, CA) for 30 min at 25°C. .. Coverslips were washed and stained using the horseradish peroxidase conjugated Vectastain ABC staining kit (Vector Laboratories) and the ImmunoPure Metal Enhanced DAB Substrate (Pierce). ..

    Article Title: The antimicrobial protein, CAP37, is upregulated in pyramidal neurons during Alzheimer’s disease
    Article Snippet: .. Following treatment, cells were fixed with formol acetone and stained using the Vectastain ABC peroxidase system (Vector Laboratories, Burlingame, CA) as previously described (Pereira et al. ). .. The rabbit anti-CAP37 and normal rabbit serum were added for 17 h. Images of stained neurons were taken at 400X magnification (Nikon TE2000, Nikon Instruments Inc., Melville, NY).

    Blocking Assay:

    Article Title: Green algae Chlamydomonas reinhardtii possess endogenous sialylated N-glycans
    Article Snippet: Briefly, proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to a polyvinylidene fluoride (PVDF) membrane. .. The membrane was washed three times with Tris-buffered saline (TBS) (50 mM Tris–HCl, pH 7.5, 150 mM NaCl) and blocked with Carbo-free blocking buffer (Cat. No. SP-5040, Vector Laboratories, Burlingame, CA) for 1 h. After blocking, the membrane was incubated in the lectin incubation buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM CaCl2 , 1 mM MgCl2 , 1 mM MnCl2 ) containing 10 μg/ml biotinylated SNA-1 (Sambucus nigra , Cat. No. BA-6802-1, EY Laboratories, San Mateo, CA) and 50 μg/ml MAA (Maackia amurensis , Cat. No. BA-7801-5, EY Laboratories) for 2 h. After three washes with TBS, the membrane was incubated with avidin plus biotinylated horseradish peroxidase (HRP) using ABC kit (Cat. No. PK-4000, Vector Laboratories) for 30 min and washed three times with TBS. .. Lectin binding to sialic acid-containing proteins was visualized using SuperSignal West Pico Chemiluminescent Substrate (Pierce, Rockford, IL).

    Incubation:

    Article Title: Green algae Chlamydomonas reinhardtii possess endogenous sialylated N-glycans
    Article Snippet: Briefly, proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to a polyvinylidene fluoride (PVDF) membrane. .. The membrane was washed three times with Tris-buffered saline (TBS) (50 mM Tris–HCl, pH 7.5, 150 mM NaCl) and blocked with Carbo-free blocking buffer (Cat. No. SP-5040, Vector Laboratories, Burlingame, CA) for 1 h. After blocking, the membrane was incubated in the lectin incubation buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM CaCl2 , 1 mM MgCl2 , 1 mM MnCl2 ) containing 10 μg/ml biotinylated SNA-1 (Sambucus nigra , Cat. No. BA-6802-1, EY Laboratories, San Mateo, CA) and 50 μg/ml MAA (Maackia amurensis , Cat. No. BA-7801-5, EY Laboratories) for 2 h. After three washes with TBS, the membrane was incubated with avidin plus biotinylated horseradish peroxidase (HRP) using ABC kit (Cat. No. PK-4000, Vector Laboratories) for 30 min and washed three times with TBS. .. Lectin binding to sialic acid-containing proteins was visualized using SuperSignal West Pico Chemiluminescent Substrate (Pierce, Rockford, IL).

    Article Title: Heparan Sulfate Proteoglycan Is an Important Attachment Factor for Cell Entry of Akabane and Schmallenberg Viruses
    Article Snippet: After washing with PBS–0.1% Tween 20 (PBS-T), biotinylated (biotin labeling kit-NH2 ; Dojindo) anti-AKAV mouse PAb was added to the wells and incubated for 30 min at room temperature. .. After washing with PBS-T, avidin-biotinylated horseradish peroxidase (HRP) complex (Vectastain ABC kit; Vector Laboratories) was added to the wells and incubated for 30 min at room temperature. ..

    Avidin-Biotin Assay:

    Article Title: Green algae Chlamydomonas reinhardtii possess endogenous sialylated N-glycans
    Article Snippet: Briefly, proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to a polyvinylidene fluoride (PVDF) membrane. .. The membrane was washed three times with Tris-buffered saline (TBS) (50 mM Tris–HCl, pH 7.5, 150 mM NaCl) and blocked with Carbo-free blocking buffer (Cat. No. SP-5040, Vector Laboratories, Burlingame, CA) for 1 h. After blocking, the membrane was incubated in the lectin incubation buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM CaCl2 , 1 mM MgCl2 , 1 mM MnCl2 ) containing 10 μg/ml biotinylated SNA-1 (Sambucus nigra , Cat. No. BA-6802-1, EY Laboratories, San Mateo, CA) and 50 μg/ml MAA (Maackia amurensis , Cat. No. BA-7801-5, EY Laboratories) for 2 h. After three washes with TBS, the membrane was incubated with avidin plus biotinylated horseradish peroxidase (HRP) using ABC kit (Cat. No. PK-4000, Vector Laboratories) for 30 min and washed three times with TBS. .. Lectin binding to sialic acid-containing proteins was visualized using SuperSignal West Pico Chemiluminescent Substrate (Pierce, Rockford, IL).

    Article Title: Heparan Sulfate Proteoglycan Is an Important Attachment Factor for Cell Entry of Akabane and Schmallenberg Viruses
    Article Snippet: After washing with PBS–0.1% Tween 20 (PBS-T), biotinylated (biotin labeling kit-NH2 ; Dojindo) anti-AKAV mouse PAb was added to the wells and incubated for 30 min at room temperature. .. After washing with PBS-T, avidin-biotinylated horseradish peroxidase (HRP) complex (Vectastain ABC kit; Vector Laboratories) was added to the wells and incubated for 30 min at room temperature. ..

    Article Title: Cortical connectivity maps reveal anatomically distinct areas in the parietal cortex of the rat
    Article Snippet: A second series was mounted and stained with Cresyl violet. .. This procedure involved processing tissue with anti-parvalbumin antibody, incubating the tissue in avidin and biotinylated horseradish peroxidase (Vectastain ABC, Vector Laboratories, Burlington, ON) and lastly subjecting the sections to a diaminobenzidine (DAB) reaction. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Vector Laboratories avidin biotin complex solution
    Avidin Biotin Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin complex solution/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex solution - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories abc kit
    Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abc kit/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    abc kit - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    Image Search Results