fetuin a levels  (Boster Bio)

 
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    Name:
    Human Fetuin B PicoKine ELISA Kit
    Description:
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Fetuin B 96wells kit with removable strips
    Catalog Number:
    EK1498
    Price:
    369.0
    Applications:
    ELISA
    Conjugate:
    No
    Immunogen:
    Expression system for standard: NSO; Immunogen sequence: C16-P382
    Size:
    96wells kit with removable strips
    Category:
    ELISA Kits
    Reactivity:
    Human
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    Structured Review

    Boster Bio fetuin a levels
    Fetuin A in supernatants of HepG2 cell cultures treated with proinflammatory cytokines. Human hepatocellular carcinoma HepG2 cells were cultured at 37 °C with 5 % CO 2 in high-glucose DMEM medium containing 10 % fetal bovine serum, 100 units/mL penicillin, and 100 μg/mL streptomycin in 6-well plates at a density of 0.25 × 10 6 cells/mL until about 70 % confluence and old medium was replaced with fresh medium. Cell monolayers were then treated with rhTNF-α (50 ng/mL), rhIL-1β (10 ng/mL), rhIFN-γ (50 ng/mL), rhMCP-1 (40 ng/mL), and rhIL-6 (100 ng/mL) and incubated at 37 °C for 24 h. Cell supernatants were collected and <t>fetuin-A</t> levels were measured using sandwich high-sensitivity ELISA (Human fetuin-A PicoKine TM ELISA kit, Boster Biological Technology, USA) following the manufacturer’s instructions as described in Patients and Methods. Fetuin-A production (mean ± SEM) was found to be significantly suppressed in HepG2 cells treated with TNF-α (82.05 ± 1.16 ng/mL, P = 0.002), IL-1β (82.73 ± 1.45 ng/mL, P = 0.003), and IFN-γ (85.70 ± 1.93 ng/mL, P = 0.02) as compared with untreated control (95.73 ± 1.43 ng/mL). However, fetuin-A production in cells treated with MCP-1 (84.74 ± 5.02 ng/mL, P = 0.10) and IL-6 (91.66 ± 2.55 ng/mL, P = 0.24) differed non-sidnificantly from control. The representative data from three independent determinations are shown
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Fetuin B 96wells kit with removable strips
    https://www.bioz.com/result/fetuin a levels/product/Boster Bio
    Average 90 stars, based on 440 article reviews
    Price from $9.99 to $1999.99
    fetuin a levels - by Bioz Stars, 2020-09
    90/100 stars

    Images

    1) Product Images from "Plasma fetuin-A/α2-HS-glycoprotein correlates negatively with inflammatory cytokines, chemokines and activation biomarkers in individuals with type-2 diabetes"

    Article Title: Plasma fetuin-A/α2-HS-glycoprotein correlates negatively with inflammatory cytokines, chemokines and activation biomarkers in individuals with type-2 diabetes

    Journal: BMC Immunology

    doi: 10.1186/s12865-016-0171-y

    Fetuin A in supernatants of HepG2 cell cultures treated with proinflammatory cytokines. Human hepatocellular carcinoma HepG2 cells were cultured at 37 °C with 5 % CO 2 in high-glucose DMEM medium containing 10 % fetal bovine serum, 100 units/mL penicillin, and 100 μg/mL streptomycin in 6-well plates at a density of 0.25 × 10 6 cells/mL until about 70 % confluence and old medium was replaced with fresh medium. Cell monolayers were then treated with rhTNF-α (50 ng/mL), rhIL-1β (10 ng/mL), rhIFN-γ (50 ng/mL), rhMCP-1 (40 ng/mL), and rhIL-6 (100 ng/mL) and incubated at 37 °C for 24 h. Cell supernatants were collected and fetuin-A levels were measured using sandwich high-sensitivity ELISA (Human fetuin-A PicoKine TM ELISA kit, Boster Biological Technology, USA) following the manufacturer’s instructions as described in Patients and Methods. Fetuin-A production (mean ± SEM) was found to be significantly suppressed in HepG2 cells treated with TNF-α (82.05 ± 1.16 ng/mL, P = 0.002), IL-1β (82.73 ± 1.45 ng/mL, P = 0.003), and IFN-γ (85.70 ± 1.93 ng/mL, P = 0.02) as compared with untreated control (95.73 ± 1.43 ng/mL). However, fetuin-A production in cells treated with MCP-1 (84.74 ± 5.02 ng/mL, P = 0.10) and IL-6 (91.66 ± 2.55 ng/mL, P = 0.24) differed non-sidnificantly from control. The representative data from three independent determinations are shown
    Figure Legend Snippet: Fetuin A in supernatants of HepG2 cell cultures treated with proinflammatory cytokines. Human hepatocellular carcinoma HepG2 cells were cultured at 37 °C with 5 % CO 2 in high-glucose DMEM medium containing 10 % fetal bovine serum, 100 units/mL penicillin, and 100 μg/mL streptomycin in 6-well plates at a density of 0.25 × 10 6 cells/mL until about 70 % confluence and old medium was replaced with fresh medium. Cell monolayers were then treated with rhTNF-α (50 ng/mL), rhIL-1β (10 ng/mL), rhIFN-γ (50 ng/mL), rhMCP-1 (40 ng/mL), and rhIL-6 (100 ng/mL) and incubated at 37 °C for 24 h. Cell supernatants were collected and fetuin-A levels were measured using sandwich high-sensitivity ELISA (Human fetuin-A PicoKine TM ELISA kit, Boster Biological Technology, USA) following the manufacturer’s instructions as described in Patients and Methods. Fetuin-A production (mean ± SEM) was found to be significantly suppressed in HepG2 cells treated with TNF-α (82.05 ± 1.16 ng/mL, P = 0.002), IL-1β (82.73 ± 1.45 ng/mL, P = 0.003), and IFN-γ (85.70 ± 1.93 ng/mL, P = 0.02) as compared with untreated control (95.73 ± 1.43 ng/mL). However, fetuin-A production in cells treated with MCP-1 (84.74 ± 5.02 ng/mL, P = 0.10) and IL-6 (91.66 ± 2.55 ng/mL, P = 0.24) differed non-sidnificantly from control. The representative data from three independent determinations are shown

    Techniques Used: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay

    2) Product Images from "Plasma fetuin-A/α2-HS-glycoprotein correlates negatively with inflammatory cytokines, chemokines and activation biomarkers in individuals with type-2 diabetes"

    Article Title: Plasma fetuin-A/α2-HS-glycoprotein correlates negatively with inflammatory cytokines, chemokines and activation biomarkers in individuals with type-2 diabetes

    Journal: BMC Immunology

    doi: 10.1186/s12865-016-0171-y

    Fetuin A in supernatants of HepG2 cell cultures treated with proinflammatory cytokines. Human hepatocellular carcinoma HepG2 cells were cultured at 37 °C with 5 % CO 2 in high-glucose DMEM medium containing 10 % fetal bovine serum, 100 units/mL penicillin, and 100 μg/mL streptomycin in 6-well plates at a density of 0.25 × 10 6 cells/mL until about 70 % confluence and old medium was replaced with fresh medium. Cell monolayers were then treated with rhTNF-α (50 ng/mL), rhIL-1β (10 ng/mL), rhIFN-γ (50 ng/mL), rhMCP-1 (40 ng/mL), and rhIL-6 (100 ng/mL) and incubated at 37 °C for 24 h. Cell supernatants were collected and fetuin-A levels were measured using sandwich high-sensitivity ELISA (Human fetuin-A PicoKine TM ELISA kit, Boster Biological Technology, USA) following the manufacturer’s instructions as described in Patients and Methods. Fetuin-A production (mean ± SEM) was found to be significantly suppressed in HepG2 cells treated with TNF-α (82.05 ± 1.16 ng/mL, P = 0.002), IL-1β (82.73 ± 1.45 ng/mL, P = 0.003), and IFN-γ (85.70 ± 1.93 ng/mL, P = 0.02) as compared with untreated control (95.73 ± 1.43 ng/mL). However, fetuin-A production in cells treated with MCP-1 (84.74 ± 5.02 ng/mL, P = 0.10) and IL-6 (91.66 ± 2.55 ng/mL, P = 0.24) differed non-sidnificantly from control. The representative data from three independent determinations are shown
    Figure Legend Snippet: Fetuin A in supernatants of HepG2 cell cultures treated with proinflammatory cytokines. Human hepatocellular carcinoma HepG2 cells were cultured at 37 °C with 5 % CO 2 in high-glucose DMEM medium containing 10 % fetal bovine serum, 100 units/mL penicillin, and 100 μg/mL streptomycin in 6-well plates at a density of 0.25 × 10 6 cells/mL until about 70 % confluence and old medium was replaced with fresh medium. Cell monolayers were then treated with rhTNF-α (50 ng/mL), rhIL-1β (10 ng/mL), rhIFN-γ (50 ng/mL), rhMCP-1 (40 ng/mL), and rhIL-6 (100 ng/mL) and incubated at 37 °C for 24 h. Cell supernatants were collected and fetuin-A levels were measured using sandwich high-sensitivity ELISA (Human fetuin-A PicoKine TM ELISA kit, Boster Biological Technology, USA) following the manufacturer’s instructions as described in Patients and Methods. Fetuin-A production (mean ± SEM) was found to be significantly suppressed in HepG2 cells treated with TNF-α (82.05 ± 1.16 ng/mL, P = 0.002), IL-1β (82.73 ± 1.45 ng/mL, P = 0.003), and IFN-γ (85.70 ± 1.93 ng/mL, P = 0.02) as compared with untreated control (95.73 ± 1.43 ng/mL). However, fetuin-A production in cells treated with MCP-1 (84.74 ± 5.02 ng/mL, P = 0.10) and IL-6 (91.66 ± 2.55 ng/mL, P = 0.24) differed non-sidnificantly from control. The representative data from three independent determinations are shown

    Techniques Used: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Plasma fetuin-A/α2-HS-glycoprotein correlates negatively with inflammatory cytokines, chemokines and activation biomarkers in individuals with type-2 diabetes
    Article Snippet: .. Fetuin-A levels were measured in cell supernatants using sandwich high-sensitivity ELISA (Sensitivity: < 10 pg/mL; Human fetuin-A PicoKine™ ELISA kit, Boster Biological Technology, USA) following the manufacturer’s instructions. .. Briefly, after adding samples (100 μL) and standards (100 μL), plates were incubated at 37 °C for 90 min. Then, without washing, biotinylated anti-human fetuin-A antibody (1:100 diluted; 130 μL/well) was added and incubated at 37 °C for 60 min. Plates were washed 3 times with 0.01 M Tris-buffered saline (TBS), then Avidin-Biotin-Peroxidase Complex (ABC) was added (1:100 diluted; 130 μL/well) and incubated at 37 °C for 30 min. Plates were washed 5 times with TBS buffer and after adding HRP substrate 3,3',5,5'-Tetramethylbenzidine (TMB color developing agent), plates were incubated at 37 °C in dark for 20–25 min. Color development was stopped by adding TMB stop solution (100 μL/well) and absorbance/optical density (OD) was read at 450 nm wavelength.

    Article Title: Plasma fetuin-A/α2-HS-glycoprotein correlates negatively with inflammatory cytokines, chemokines and activation biomarkers in individuals with type-2 diabetes
    Article Snippet: .. Cell supernatants were collected and fetuin-A levels were measured using sandwich high-sensitivity ELISA (Human fetuin-A PicoKineTM ELISA kit, Boster Biological Technology, USA) following the manufacturer’s instructions as described in Patients and Methods. ..

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    Boster Bio avidin biotin peroxidase complex solution
    Avidin Biotin Peroxidase Complex Solution, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin peroxidase complex solution/product/Boster Bio
    Average 91 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex solution - by Bioz Stars, 2020-09
    91/100 stars
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