immpact dab peroxidase  (Vector Laboratories)


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    Name:
    ImmPACT DAB Peroxidase HRP Substrate
    Description:
    ImmPACT DAB Peroxidase Substrate is 2 4 fold greater sensitivity than original substrate kitOptimized for IHC ICC ISH and blotsHeat StablePermanent mountingIdeal for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and concentrated DAB stock solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This product is an entirely new formula for a diaminobenzidine based peroxidase substrate ImmPACT DAB produces a dark brown reaction product that is crisper and is 3 4 times more sensitive than the original Vector DAB substrate kit It can be used for both manual and automated staining methods The ImmPACT DAB working solution is stable for at least five days at room temperature and at least two weeks when stored at 4° C This product consists of 120 ml of diluent and a convenient concentrated stock solution of highly purified DAB This product can also be used on blots
    Catalog Number:
    sk-4105
    Price:
    None
    Size:
    120 ml
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Buy from Supplier


    Structured Review

    Vector Laboratories immpact dab peroxidase
    ImmPACT DAB Peroxidase HRP Substrate
    ImmPACT DAB Peroxidase Substrate is 2 4 fold greater sensitivity than original substrate kitOptimized for IHC ICC ISH and blotsHeat StablePermanent mountingIdeal for single and multiple labelingWorking solutions stable for up to 14 days at 4 °COne year expiry dateSupplied as 120 ml of diluent and concentrated DAB stock solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This product is an entirely new formula for a diaminobenzidine based peroxidase substrate ImmPACT DAB produces a dark brown reaction product that is crisper and is 3 4 times more sensitive than the original Vector DAB substrate kit It can be used for both manual and automated staining methods The ImmPACT DAB working solution is stable for at least five days at room temperature and at least two weeks when stored at 4° C This product consists of 120 ml of diluent and a convenient concentrated stock solution of highly purified DAB This product can also be used on blots
    https://www.bioz.com/result/immpact dab peroxidase/product/Vector Laboratories
    Average 99 stars, based on 9913 article reviews
    Price from $9.99 to $1999.99
    immpact dab peroxidase - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators"

    Article Title: Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0135776

    Human mast cells express GMF. Cytospin smears of cultured human mast cells were analyzed first for mast cell specific tryptase using mouse anti-human mast cell tryptase by ICC (n = 3). Mast cells were then analyzed for the expression of GMF using GMF polyclonal antibody and GMF monoclonal antibody (n = 3). GMF ICC with polyclonal antibody was carried out using ImmPRESS reagent anti-rabbit Ig peroxidase, and tryptase and another GMF ICCs were carried out with monoclonal antibodies using ImmPRESS reagent anti-mouse Ig peroxidase. ImmPACT DAB peroxidase substrate kit was used for both IHCs. Presence of brown color indicates the expression of GMF in human mast cells. Isotype matched control antibodies did not show positive reaction. Original magnification = 400x.
    Figure Legend Snippet: Human mast cells express GMF. Cytospin smears of cultured human mast cells were analyzed first for mast cell specific tryptase using mouse anti-human mast cell tryptase by ICC (n = 3). Mast cells were then analyzed for the expression of GMF using GMF polyclonal antibody and GMF monoclonal antibody (n = 3). GMF ICC with polyclonal antibody was carried out using ImmPRESS reagent anti-rabbit Ig peroxidase, and tryptase and another GMF ICCs were carried out with monoclonal antibodies using ImmPRESS reagent anti-mouse Ig peroxidase. ImmPACT DAB peroxidase substrate kit was used for both IHCs. Presence of brown color indicates the expression of GMF in human mast cells. Isotype matched control antibodies did not show positive reaction. Original magnification = 400x.

    Techniques Used: Cell Culture, Immunocytochemistry, Expressing

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    Article Title: Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors
    Article Snippet: .. The incubation with avidin-peroxidase complex (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) lasted for 30 min. After staining with a chromogenic substrate containing diaminobenzidine (ImmPACT DAB Substrate, SK-4105, Vector Laboratories, USA) for 5 min, the tissue slices were stained with H & E. .. Image analysis Tissue slices were viewed by light microscopy at magnification ×400 and ×1000.

    Article Title: Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators
    Article Snippet: .. ImmPRESS reagent anti-mouse Ig peroxidase, ImmPRESS reagent anti-rabbit Ig peroxidase kits, ImmPACT DAB peroxidase and avidin-biotin complex (ABC) kits were obtained from Vector Laboratories (Burlingame, CA). .. Recombinant α-synuclein (human) was from Enzo Life Sciences (Farmingdale, NY) and used with both human and mouse mast cells.

    Immunostaining:

    Article Title: Connexin 30 deficiency attenuates A2 astrocyte responses and induces severe neurodegeneration in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine hydrochloride Parkinson’s disease animal model
    Article Snippet: .. For colorimetric immunostaining, the sections were incubated with primary antibodies against tyrosine hydroxylase (TH), dopamine transporter (DAT), GFAP, and Iba1 (Additional file : Table S1) followed by a rabbit or mouse Vectastain Elite ABC HRP kit (Vector Laboratories) and ImmPACT DAB Peroxidase Substrate (Vector Laboratories). .. We used Nikon NIS-Elements software (Nikon) to calculate the number of Iba1-positive cells, Cx30 dots, and Cx43 dots in the unilateral striata according to the previously reported procedures [ , ].

    Incubation:

    Article Title: Connexin 30 deficiency attenuates A2 astrocyte responses and induces severe neurodegeneration in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine hydrochloride Parkinson’s disease animal model
    Article Snippet: .. For colorimetric immunostaining, the sections were incubated with primary antibodies against tyrosine hydroxylase (TH), dopamine transporter (DAT), GFAP, and Iba1 (Additional file : Table S1) followed by a rabbit or mouse Vectastain Elite ABC HRP kit (Vector Laboratories) and ImmPACT DAB Peroxidase Substrate (Vector Laboratories). .. We used Nikon NIS-Elements software (Nikon) to calculate the number of Iba1-positive cells, Cx30 dots, and Cx43 dots in the unilateral striata according to the previously reported procedures [ , ].

    Article Title: A model of breast cancer heterogeneity reveals vascular mimicry as a driver of metastasis
    Article Snippet: .. After washing, the slides were incubated with secondary antibody from the previous kit for 30 min, rinsed, and developed with chromogen ImmPACT DAB Peroxidase Substrate for staining (SK-4105, Vector Laboratories) until the desired intensity was achieved. .. Slides were then stained with Periodic Acid-Schiff (PAS) Kit (Sigma) according to manufacturer's instructions.

    Article Title: The absence of the leukotriene B4 receptor BLT1 attenuates peripheral inflammation and spinal nociceptive processing following intraplantar formalin injury
    Article Snippet: .. The sections were incubated with biotinylated goat anti-rabbit IgG (1:200, Vector Laboratories, Burlingame, CA, USA) at room temperature for 1 h and then incubated in Vectastain Elite ABC Reagent (PK-6101, Vector Laboratories) for 1 h followed by incubation in a diaminobenzidine solution (ImmPACT™ DAB substrate, SK-4105, Vector Laboratories) for 1 min. ..

    Article Title: Inositol 1,4,5-triphosphate receptor is selectively expressed in cerebellum but not cerebellum-like structures of the elasmobranch fish, Leucoraja erinacea
    Article Snippet: .. Slides were incubated in ABC reagent for 30 minutes, then in a DAB peroxidase substrate (Vector Laboratories ImmPACT DAB peroxidase (HRP) substrate Catalog # SK-4105 ) for two minutes and dehydrated in an ethyl alcohol series and cleared in xylene. .. A no-primary negative control was done for each protein.

    Article Title: Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors
    Article Snippet: .. The incubation with avidin-peroxidase complex (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) lasted for 30 min. After staining with a chromogenic substrate containing diaminobenzidine (ImmPACT DAB Substrate, SK-4105, Vector Laboratories, USA) for 5 min, the tissue slices were stained with H & E. .. Image analysis Tissue slices were viewed by light microscopy at magnification ×400 and ×1000.

    Modification:

    Article Title: A Murine Orthotopic Allograft to Model Prostate Cancer Growth and Metastasis
    Article Snippet: .. Matrigel (Corning, catalog number: 354234) 1× phosphate buffered saline (PBS), pH 7.4 (Thermo Fisher Scientific, Gibco™, catalog number: 10010-023) 1× trypsin-EDTA (Mediatech, catalog number: 25-053-CI) Fluriso isoflurane (VetOne, catalog number: 501017) 10% povidone-iodine ‘Betadine’ prep solution, 16 oz. (Henry Schein, catalog number: 6906950) 50 mg/ml injectable Carprofen (Zoetis, catalog number: 07-844-7425) Ethanol, reagent alcohol 200 proof, PHARMCO-AAPER™ (Thermo Fisher Scientific, Fisher Scientific, catalog number: 16-100-824) ImmPRESS HRP Anti-Rabbit Polymer Detection Kit (Vector Labs, catalog number: MP-7401) ImmPACT DAB Peroxidase Substrate (Vector Labs, catalog number: SK-4105) Myc-CaP growth medium (see Recipes) Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, Gibco™, catalog number: 11965-092) Fetal bovine serum (Gemini Bio-Products, catalog number: 100-106) Penicillin-streptomycin (10,000 U/ml) (Thermo Fisher Scientific, Gibco™, catalog number: 15140-122) Bovine pituitary extract (BPE) (Thermo Fisher Scientific, Gibco™, catalog number: 13028-014) Recombinant human EGF (R & D Systems, catalog number: 236-EG) Bovine insulin powder (Gemini Bio-Products, catalog number: 700-112) .. Incubator Centrifuge Cell culture laminar flow hood, vacuum suction, centrifuge, cell counter 2 L induction chamber Scissors (Roboz Surgical Instrument, catalog number: RS-5914) Fine forceps (Roboz Surgical Instrument, catalog number: RS-5132) Mouse housing and handling facility Isoflurane gas anesthetic system (VetEquip, catalog number: 922100) including a vaporizer, induction chamber, tubing, nose cones, and oxygen tank Glass bead sterilizer (GERMINATOR 500) (Roboz Surgical Instrument, catalog number: DS-401) Rechargeable shaver Space gel heating pads (Braintree Scientific, catalog number: SPGL) 25 μl, model 702 RN syringe (Hamilton, catalog number: 7636-01) Syringe guide 25 μl–500 μl/7000 SYR (Hamilton, catalog number: 14906)

    Staining:

    Article Title: A model of breast cancer heterogeneity reveals vascular mimicry as a driver of metastasis
    Article Snippet: .. After washing, the slides were incubated with secondary antibody from the previous kit for 30 min, rinsed, and developed with chromogen ImmPACT DAB Peroxidase Substrate for staining (SK-4105, Vector Laboratories) until the desired intensity was achieved. .. Slides were then stained with Periodic Acid-Schiff (PAS) Kit (Sigma) according to manufacturer's instructions.

    Article Title: Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors
    Article Snippet: .. The incubation with avidin-peroxidase complex (VECTASTAIN Elite ABC Kit, PK-7200, Vector Laboratories, USA) lasted for 30 min. After staining with a chromogenic substrate containing diaminobenzidine (ImmPACT DAB Substrate, SK-4105, Vector Laboratories, USA) for 5 min, the tissue slices were stained with H & E. .. Image analysis Tissue slices were viewed by light microscopy at magnification ×400 and ×1000.

    Recombinant:

    Article Title: A Murine Orthotopic Allograft to Model Prostate Cancer Growth and Metastasis
    Article Snippet: .. Matrigel (Corning, catalog number: 354234) 1× phosphate buffered saline (PBS), pH 7.4 (Thermo Fisher Scientific, Gibco™, catalog number: 10010-023) 1× trypsin-EDTA (Mediatech, catalog number: 25-053-CI) Fluriso isoflurane (VetOne, catalog number: 501017) 10% povidone-iodine ‘Betadine’ prep solution, 16 oz. (Henry Schein, catalog number: 6906950) 50 mg/ml injectable Carprofen (Zoetis, catalog number: 07-844-7425) Ethanol, reagent alcohol 200 proof, PHARMCO-AAPER™ (Thermo Fisher Scientific, Fisher Scientific, catalog number: 16-100-824) ImmPRESS HRP Anti-Rabbit Polymer Detection Kit (Vector Labs, catalog number: MP-7401) ImmPACT DAB Peroxidase Substrate (Vector Labs, catalog number: SK-4105) Myc-CaP growth medium (see Recipes) Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, Gibco™, catalog number: 11965-092) Fetal bovine serum (Gemini Bio-Products, catalog number: 100-106) Penicillin-streptomycin (10,000 U/ml) (Thermo Fisher Scientific, Gibco™, catalog number: 15140-122) Bovine pituitary extract (BPE) (Thermo Fisher Scientific, Gibco™, catalog number: 13028-014) Recombinant human EGF (R & D Systems, catalog number: 236-EG) Bovine insulin powder (Gemini Bio-Products, catalog number: 700-112) .. Incubator Centrifuge Cell culture laminar flow hood, vacuum suction, centrifuge, cell counter 2 L induction chamber Scissors (Roboz Surgical Instrument, catalog number: RS-5914) Fine forceps (Roboz Surgical Instrument, catalog number: RS-5132) Mouse housing and handling facility Isoflurane gas anesthetic system (VetEquip, catalog number: 922100) including a vaporizer, induction chamber, tubing, nose cones, and oxygen tank Glass bead sterilizer (GERMINATOR 500) (Roboz Surgical Instrument, catalog number: DS-401) Rechargeable shaver Space gel heating pads (Braintree Scientific, catalog number: SPGL) 25 μl, model 702 RN syringe (Hamilton, catalog number: 7636-01) Syringe guide 25 μl–500 μl/7000 SYR (Hamilton, catalog number: 14906)

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  • 99
    Vector Laboratories avidin biotin peroxidase complex solution
    Avidin Biotin Peroxidase Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin peroxidase complex solution/product/Vector Laboratories
    Average 99 stars, based on 118 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex solution - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Vector Laboratories biotin avidin peroxidase complex solution
    Biotin Avidin Peroxidase Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin avidin peroxidase complex solution/product/Vector Laboratories
    Average 99 stars, based on 185 article reviews
    Price from $9.99 to $1999.99
    biotin avidin peroxidase complex solution - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories avidin biotin complex solution
    Avidin Biotin Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin complex solution/product/Vector Laboratories
    Average 99 stars, based on 182 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex solution - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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