avidin biotin complex solutions  (Vector Laboratories)


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    Name:
    Avidin Biotin Blocking Kit
    Description:
    Avidin Biotin Blocking Kit blocks all endogenous biotin biotin receptors and avidin binding sites present in tissues This kit is designed for use with avidin detection systems such as the VECTASTAIN ABC Kits if avidin or biotinylated reagents bind non specifically to tissues or proteins This blocking kit consists of 18 ml of Avidin D and 18 ml of biotin in convenient dropper bottles
    Catalog Number:
    sp-2001
    Price:
    None
    Size:
    1 Kit
    Category:
    Histology reagents or solutions or stains
    Buy from Supplier


    Structured Review

    Vector Laboratories avidin biotin complex solutions
    Avidin Biotin Blocking Kit
    Avidin Biotin Blocking Kit blocks all endogenous biotin biotin receptors and avidin binding sites present in tissues This kit is designed for use with avidin detection systems such as the VECTASTAIN ABC Kits if avidin or biotinylated reagents bind non specifically to tissues or proteins This blocking kit consists of 18 ml of Avidin D and 18 ml of biotin in convenient dropper bottles
    https://www.bioz.com/result/avidin biotin complex solutions/product/Vector Laboratories
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex solutions - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Effects of Estrogen Therapy on the Serotonergic System in an Animal Model of Perimenopause Induced by 4-Vinylcyclohexen Diepoxide (VCD)
    Article Snippet: .. Signal amplification was performed using an avidin-biotin kit (1:100; Vector Laboratories) for 1 h at room temperature. ..

    Avidin-Biotin Assay:

    Article Title: Mechanistic insight into hyaluronic acid and platelet-rich plasma-mediated anti-inflammatory and anti-apoptotic activities in osteoarthritic mice
    Article Snippet: .. Avidin and biotin binding sites contained in the tissue samples were blocked using a commercial avidin-biotin blocking kit (Vector laboratories). .. Sections were then incubated for 30 minutes at room temperature with the following mouse anti-human monoclonal antibodies diluted in phosphate buffered saline (PBS) containing BSA; anti- MMP-1 (1:40 dilution) and incubated at 4°C for overnight (Calbiochem, Oncogene Science Inc. Cambridge).

    Article Title: Hepatic acute phase response protects the brain from focal inflammation during postnatal window of susceptibility
    Article Snippet: .. These were separated by an avidin/biotin blocking (Avidin/Biotin blocking kit, Vector Laboratories, UK). .. A citrate buffer (10 mM Citric Acid, 0.05% Tween 20, pH 6.0) antigen retrieval step was added to the laminin staining protocol.

    Article Title: ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer
    Article Snippet: .. Endogenous biotin was blocked using an avidin/biotin blocking kit (Vector Labs), followed by a 30-min protein-blocking step with serum-free protein block (Life Technologies) at 37°C. .. Sections were incubated with anti-ETV7 antibody overnight at 4°C.

    Article Title: Effects of Estrogen Therapy on the Serotonergic System in an Animal Model of Perimenopause Induced by 4-Vinylcyclohexen Diepoxide (VCD)
    Article Snippet: .. Signal amplification was performed using an avidin-biotin kit (1:100; Vector Laboratories) for 1 h at room temperature. ..

    Article Title: GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment
    Article Snippet: .. Sections were blocked with normal serum for 1 h at room temperature and endogenous biotin activity and avidin binding sites were blocked with avidin–biotin blocking kit according to manufacturer's instructions (Vector laboratories, SP‐2001). .. Primary antibody dilutions are as follows: pMLC‐2 (Cell Signaling, 3671, 1/200), α‐SMA (Abcam, ab5694, 1/300), CD68 (AbD Serotec, MCA1957GA, 1/500), YAP (Santa Cruz sc‐101199, 1/100).

    Article Title: Na+/Ca2+ Exchanger 1 in Airway Smooth Muscle of Allergic Inflammation Mouse Model
    Article Snippet: .. They were then treated with and Avidin/Biotin blocking kit (Vector Labs; Burlingame, CA, United States) and then incubated with 1:200 dilution R3F1 primary or isotype control antibody at 4°C overnight, followed by a biotin-conjugated anti-mouse secondary antibody (Jackson Immunoresearch; Westgrove, PA, United States) for 1 h. Samples were then washed and incubated with Alexa fluor 488 labeled streptavidin (Invitrogen, Carlsbad, CA, United States) for 1 h. The samples were mounted in an aqueous mounting solution containing DAPI. .. The samples were examined with a Nikon i80 fluorescent microscope fitted with a low light digital camera and image analysis software.

    Labeling:

    Article Title: Na+/Ca2+ Exchanger 1 in Airway Smooth Muscle of Allergic Inflammation Mouse Model
    Article Snippet: .. They were then treated with and Avidin/Biotin blocking kit (Vector Labs; Burlingame, CA, United States) and then incubated with 1:200 dilution R3F1 primary or isotype control antibody at 4°C overnight, followed by a biotin-conjugated anti-mouse secondary antibody (Jackson Immunoresearch; Westgrove, PA, United States) for 1 h. Samples were then washed and incubated with Alexa fluor 488 labeled streptavidin (Invitrogen, Carlsbad, CA, United States) for 1 h. The samples were mounted in an aqueous mounting solution containing DAPI. .. The samples were examined with a Nikon i80 fluorescent microscope fitted with a low light digital camera and image analysis software.

    Incubation:

    Article Title: Na+/Ca2+ Exchanger 1 in Airway Smooth Muscle of Allergic Inflammation Mouse Model
    Article Snippet: .. They were then treated with and Avidin/Biotin blocking kit (Vector Labs; Burlingame, CA, United States) and then incubated with 1:200 dilution R3F1 primary or isotype control antibody at 4°C overnight, followed by a biotin-conjugated anti-mouse secondary antibody (Jackson Immunoresearch; Westgrove, PA, United States) for 1 h. Samples were then washed and incubated with Alexa fluor 488 labeled streptavidin (Invitrogen, Carlsbad, CA, United States) for 1 h. The samples were mounted in an aqueous mounting solution containing DAPI. .. The samples were examined with a Nikon i80 fluorescent microscope fitted with a low light digital camera and image analysis software.

    Activity Assay:

    Article Title: GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment
    Article Snippet: .. Sections were blocked with normal serum for 1 h at room temperature and endogenous biotin activity and avidin binding sites were blocked with avidin–biotin blocking kit according to manufacturer's instructions (Vector laboratories, SP‐2001). .. Primary antibody dilutions are as follows: pMLC‐2 (Cell Signaling, 3671, 1/200), α‐SMA (Abcam, ab5694, 1/300), CD68 (AbD Serotec, MCA1957GA, 1/500), YAP (Santa Cruz sc‐101199, 1/100).

    Blocking Assay:

    Article Title: Ultrastructural Visualization of 3D Chromatin Folding Using Serial Block-Face Scanning Electron Microscopy and In Situ Hybridization (3D-EMISH)
    Article Snippet: .. After blocking of endogenous biotin (Vector Laboratories; SP-2001), the cells were permeabilized with 0.5% Triton X-100 (Sigma) in PBS (RT, 20 min). .. To augment permeabilization, the cells were immersed in a cryoprotectant solution (20% glycerol in PBS, RT, 2h) followed by their repeated freezing-thawing above the surface of the liquid nitrogen (4 × 30 sec).

    Article Title: Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization
    Article Snippet: .. In details, after blocking of endogenous biotin (Vector Laboratories; SP-2001), the cells were permeabilized with 0.5% Triton X-100 (Sigma) in PBS (RT, 20 min). ..

    Article Title: Mechanistic insight into hyaluronic acid and platelet-rich plasma-mediated anti-inflammatory and anti-apoptotic activities in osteoarthritic mice
    Article Snippet: .. Avidin and biotin binding sites contained in the tissue samples were blocked using a commercial avidin-biotin blocking kit (Vector laboratories). .. Sections were then incubated for 30 minutes at room temperature with the following mouse anti-human monoclonal antibodies diluted in phosphate buffered saline (PBS) containing BSA; anti- MMP-1 (1:40 dilution) and incubated at 4°C for overnight (Calbiochem, Oncogene Science Inc. Cambridge).

    Article Title: Hepatic acute phase response protects the brain from focal inflammation during postnatal window of susceptibility
    Article Snippet: .. These were separated by an avidin/biotin blocking (Avidin/Biotin blocking kit, Vector Laboratories, UK). .. A citrate buffer (10 mM Citric Acid, 0.05% Tween 20, pH 6.0) antigen retrieval step was added to the laminin staining protocol.

    Article Title: ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer
    Article Snippet: .. Endogenous biotin was blocked using an avidin/biotin blocking kit (Vector Labs), followed by a 30-min protein-blocking step with serum-free protein block (Life Technologies) at 37°C. .. Sections were incubated with anti-ETV7 antibody overnight at 4°C.

    Article Title: GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment
    Article Snippet: .. Sections were blocked with normal serum for 1 h at room temperature and endogenous biotin activity and avidin binding sites were blocked with avidin–biotin blocking kit according to manufacturer's instructions (Vector laboratories, SP‐2001). .. Primary antibody dilutions are as follows: pMLC‐2 (Cell Signaling, 3671, 1/200), α‐SMA (Abcam, ab5694, 1/300), CD68 (AbD Serotec, MCA1957GA, 1/500), YAP (Santa Cruz sc‐101199, 1/100).

    Article Title: Na+/Ca2+ Exchanger 1 in Airway Smooth Muscle of Allergic Inflammation Mouse Model
    Article Snippet: .. They were then treated with and Avidin/Biotin blocking kit (Vector Labs; Burlingame, CA, United States) and then incubated with 1:200 dilution R3F1 primary or isotype control antibody at 4°C overnight, followed by a biotin-conjugated anti-mouse secondary antibody (Jackson Immunoresearch; Westgrove, PA, United States) for 1 h. Samples were then washed and incubated with Alexa fluor 488 labeled streptavidin (Invitrogen, Carlsbad, CA, United States) for 1 h. The samples were mounted in an aqueous mounting solution containing DAPI. .. The samples were examined with a Nikon i80 fluorescent microscope fitted with a low light digital camera and image analysis software.

    Binding Assay:

    Article Title: Mechanistic insight into hyaluronic acid and platelet-rich plasma-mediated anti-inflammatory and anti-apoptotic activities in osteoarthritic mice
    Article Snippet: .. Avidin and biotin binding sites contained in the tissue samples were blocked using a commercial avidin-biotin blocking kit (Vector laboratories). .. Sections were then incubated for 30 minutes at room temperature with the following mouse anti-human monoclonal antibodies diluted in phosphate buffered saline (PBS) containing BSA; anti- MMP-1 (1:40 dilution) and incubated at 4°C for overnight (Calbiochem, Oncogene Science Inc. Cambridge).

    Article Title: GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment
    Article Snippet: .. Sections were blocked with normal serum for 1 h at room temperature and endogenous biotin activity and avidin binding sites were blocked with avidin–biotin blocking kit according to manufacturer's instructions (Vector laboratories, SP‐2001). .. Primary antibody dilutions are as follows: pMLC‐2 (Cell Signaling, 3671, 1/200), α‐SMA (Abcam, ab5694, 1/300), CD68 (AbD Serotec, MCA1957GA, 1/500), YAP (Santa Cruz sc‐101199, 1/100).

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  • 93
    Vector Laboratories avidin biotin complex solution
    Avidin Biotin Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin complex solution/product/Vector Laboratories
    Average 93 stars, based on 182 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex solution - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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