avidin biotin complex solution  (Vector Laboratories)


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    Name:
    VECTASTAIN Elite ABC HRP Kit Peroxidase Standard
    Description:
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Components 2 ml Reagent A2 ml Reagent BReagent A and B when mixed form the ABC peroxidase complex Kit contains sufficient reagents to stain 500 1000 sections The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    Catalog Number:
    pk-6100
    Price:
    None
    Size:
    1 Kit
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    No antibody included
    Buy from Supplier


    Structured Review

    Vector Laboratories avidin biotin complex solution
    VECTASTAIN Elite ABC HRP Kit Peroxidase Standard
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Components 2 ml Reagent A2 ml Reagent BReagent A and B when mixed form the ABC peroxidase complex Kit contains sufficient reagents to stain 500 1000 sections The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    https://www.bioz.com/result/avidin biotin complex solution/product/Vector Laboratories
    Average 99 stars, based on 182 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex solution - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Produced:

    Article Title: The Importance of Titrating Antibodies for Immunocytochemical Methods
    Article Snippet: .. Brain sections as obtained in Basic Protocol 1, steps 1 to 15 Primary antibody Biotinylated secondary antibody Vectastain Elite ABC Kit (Standard; Vector Laboratories, cat. no. PK-6100) including solution A and Solution B Biotinylated tyramine produced as outlined in . .. This reagent can be purchased from a number of suppliers (such as ThermoFisher Scientific) but concentrations must be determined empirically.

    Staining:

    Article Title: Expression and Function of Chemokine Receptors on Human Thymocytes: Implications for Infection by Human Immunodeficiency Virus Type 1
    Article Snippet: .. The staining was developed with a Vectastain Elite ABC kit with 3-amino-9-ethylcarbazole substrate (Vector Labs). .. Sections were counterstained with hematoxylin and mounted with Crystal/Mount (both from Biomeda Corp., Foster City, Calif.).

    Immunohistochemistry:

    Article Title: Modulation of lethal HPAIV H5N8 clade 2.3.4.4B infection in AIV pre-exposed mallards
    Article Snippet: .. Immunohistochemistry was employed to detect influenza A virus matrix protein using the avidin–biotin-peroxidase-complex method (Vectastain PK 6100; Vector Laboratories, Burlingame, CA, USA) with citric buffer (10 mM, pH 6.0) pretreatment, a monoclonal antibody (mAb) directed against an epitope of the influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (Agilent Technologies, Santa Clara, CA, USA), and hematoxylin counterstain [ ]. ..

    Avidin-Biotin Assay:

    Article Title: Modulation of lethal HPAIV H5N8 clade 2.3.4.4B infection in AIV pre-exposed mallards
    Article Snippet: .. Immunohistochemistry was employed to detect influenza A virus matrix protein using the avidin–biotin-peroxidase-complex method (Vectastain PK 6100; Vector Laboratories, Burlingame, CA, USA) with citric buffer (10 mM, pH 6.0) pretreatment, a monoclonal antibody (mAb) directed against an epitope of the influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (Agilent Technologies, Santa Clara, CA, USA), and hematoxylin counterstain [ ]. ..

    Article Title: Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan
    Article Snippet: .. A Vectastain Elite kit was used for secondary antibody and Avidin–Biotin Complex (ABC) at RT in accordance with the manufacturer's instructions (Vector laboratories, Burlingame, USA). .. Secondary antibody was added for 30 min followed by a wash and incubation with ABC for another 30 min. 3,3΄-diaminobenzidine (DAB) (Vector laboratories) was used to stain slides for 2 min and colouring reaction stopped using distilled water.

    Article Title: Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats
    Article Snippet: .. Immunohistology was performed using a mouse monoclonal antibody against the RVFV Gc-protein (clone: GC9A9) [ ], the avidin–biotin–peroxidase complex method (ABC, Elite PK6100; Vector Laboratories, Burlingame, CA, USA) with 3-amino-9-ethylcarbazole (AEC, Dako, Glostrup, Denmark) as chromogen and hematoxylin counterstain. ..

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  • 91
    Vector Laboratories avidin biotin peroxidase complex solution
    Avidin Biotin Peroxidase Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 91/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin peroxidase complex solution/product/Vector Laboratories
    Average 91 stars, based on 118 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex solution - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    93
    Vector Laboratories avidin biotin complex solution
    Avidin Biotin Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin complex solution/product/Vector Laboratories
    Average 93 stars, based on 182 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex solution - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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