avidin biotin complex solution  (Vector Laboratories)


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    Name:
    Avidin D Alkaline Phosphatase labeled
    Description:
    Alkaline Phosphatase Avidin D reagent is a powerful tool to detect or purify biotinylated proteins nucleic acids and other macromolecules Vector Laboratories enzyme conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios Specific covalent linkages are chosen to provide stable highly active conjugates These enzyme conjugates are suitable for use in solid phase assays tissue cell staining systems and blotting applications One unit is 1 mole p nitrophenylphosphate hydrolyzed per min at 37 C pH 9 8
    Catalog Number:
    A-2100
    Price:
    None
    Category:
    Proteins
    Size:
    100U
    Buy from Supplier


    Structured Review

    Vector Laboratories avidin biotin complex solution
    Avidin D Alkaline Phosphatase labeled
    Alkaline Phosphatase Avidin D reagent is a powerful tool to detect or purify biotinylated proteins nucleic acids and other macromolecules Vector Laboratories enzyme conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios Specific covalent linkages are chosen to provide stable highly active conjugates These enzyme conjugates are suitable for use in solid phase assays tissue cell staining systems and blotting applications One unit is 1 mole p nitrophenylphosphate hydrolyzed per min at 37 C pH 9 8
    https://www.bioz.com/result/avidin biotin complex solution/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex solution - by Bioz Stars, 2021-04
    97/100 stars

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    Incubation:

    Article Title: Neuronal responses to transient hypoglycaemia in the dorsal vagal complex of the rat brainstem
    Article Snippet: .. Sections were then transferred into Tris-buffered saline (TBS), washed 3 ×, blocked with casein solution (Vector Laboratories Ltd, Peterborough, UK) in TBS for 30 min at RT and incubated overnight at 4°C with avidin-conjugated alkaline phosphatase (Vector Laboratories). ..

    Article Title: Inhibition of Choroidal Neovascularization by Intravenous Injection of Adenoviral Vectors Expressing Secretable Endostatin
    Article Snippet: Slides were incubated in methanol/H2 O2 for 10 minutes at 4°C, washed with 0.05 mol/L Tris-buffered saline, pH 7.6 (TBS), and incubated for 30 minutes in 10% normal porcine serum. .. Slides were incubated 2 hours at room temperature with biotinylated GSA and after rinsing with 0.05 mol/L TBS, they were incubated with avidin coupled to alkaline phosphatase (Vector Laboratories) for 45 minutes at room temperature. .. After being washed for 10 minutes with 0.05 mol/L TBS, slides were incubated with Histomark Red (Kirkegaard and Perry) to give a red reaction product that is distinguishable from melanin.

    Article Title: Induction of cytokines and anti-cytokine autoantibodies in cerebrospinal fluid (CSF) during experimental bacterial meningitis
    Article Snippet: .. After another 1 h of incubation at 37°C and 10 washes, 100 μl of avidin-biotin alkaline phosphatase complex (ABC-AP; Vector Labs, Burlingame, CA) diluted 1:100 in PBS were added for 30 min. Unbound ABC-AP was removed by five consecutive washings with PBS, and 100 μl/well of freshly prepared enzyme substrate solution were added. .. Absorbance was measured after 15 min incubation in the dark in a 405 Multiscan photometer (mcc/340; Labsystem, Helsinki, Finland).

    Article Title: A New Cell Enzyme-Linked Immunosorbent Assay Demonstrates Gamma Interferon Suppression by Beta Interferon in Multiple Sclerosis
    Article Snippet: To detect any captured IFN-γ, 100 μl of biotinylated anti-IFN-γ (7B-B6-1) MAb (0.5 μg/ml; Mabtech) diluted in PBS containing 0.5% Tween 20 and 2% bovine serum albumin was added to the wells. .. After another 60 min of incubation at 37°C and five washes, 100 μl of avidin-biotin alkaline phosphatase complex (Vector Laboratories, Burlingame, Calif.) diluted 1:100 in PBS was added for 45 min. Unbound avidin-biotin alkaline phosphatase complex was removed by five consecutive washings with Tween-PBS, and 100 μl of freshly prepared enzyme substrate solution was added to each well. .. Absorbance was measured after 15 min of incubation in the dark in a 405 Multiscan photometer (mcc/340; Labsystem, Helsinki, Finland).

    Article Title: HIV Nef Protein Causes Endothelial Dysfunction in Porcine Pulmonary Arteries and Human Pulmonary Artery Endothelial Cells
    Article Snippet: .. After washing with PBS, the sections were incubated with biotinylated secondary antibody for 30 min. An avidin-biotin reaction using peroxidase enzyme was used for protein detection (ABC kit; Vector Laboratories, Burlingham, CA). .. Immune complexes were detected with diaminobenzidine under the microscope before counterstaining with hematoxylin for 2 min.

    Avidin-Biotin Assay:

    Article Title: Neuronal responses to transient hypoglycaemia in the dorsal vagal complex of the rat brainstem
    Article Snippet: .. Sections were then transferred into Tris-buffered saline (TBS), washed 3 ×, blocked with casein solution (Vector Laboratories Ltd, Peterborough, UK) in TBS for 30 min at RT and incubated overnight at 4°C with avidin-conjugated alkaline phosphatase (Vector Laboratories). ..

    Article Title: Inhibition of Choroidal Neovascularization by Intravenous Injection of Adenoviral Vectors Expressing Secretable Endostatin
    Article Snippet: Slides were incubated in methanol/H2 O2 for 10 minutes at 4°C, washed with 0.05 mol/L Tris-buffered saline, pH 7.6 (TBS), and incubated for 30 minutes in 10% normal porcine serum. .. Slides were incubated 2 hours at room temperature with biotinylated GSA and after rinsing with 0.05 mol/L TBS, they were incubated with avidin coupled to alkaline phosphatase (Vector Laboratories) for 45 minutes at room temperature. .. After being washed for 10 minutes with 0.05 mol/L TBS, slides were incubated with Histomark Red (Kirkegaard and Perry) to give a red reaction product that is distinguishable from melanin.

    Article Title: Expression of ?F508 Cystic Fibrosis Transmembrane Regulator (CFTR) Decreases Membrane Sialylation
    Article Snippet: We used a combination of ELISA, immunocytochemistry and FACS to demonstrate the differential presence of sialic acid residues recognized by specific lectins and quantitative sialic acid analysis, and to determine whether the ΔF508 mutation of the CFTR is responsible for the sialylation defects noted in this common form of cystic fibrosis. .. Reagents Fluorescein and Biotin conjugates of Maackia amaurensis lectin (MAL II), avidin D-alkaline phosphatase, Vector Laboratories (Burlingame, CA); biotinlylated conjugate of cholera toxin, List Biological Lab, Campbell, CA. .. ; N-acetylneuraminic acid and N-glycolylneuraminic acid1,2 diamino-4,5-methylenedioxybenzene, N-propyl gallate, N-ethylmaleimide, PMSF, leupeptin, TBST, goat anti-rabbit alkaline phosphatase, Sigma Chemical Company, St. Louis, MO; Biorad protein standard assay, alkaline phosphatase substrate kit (p-nitrophenylphosphate, diethanolamine buffer), BioRad, Hercules, CA.

    Article Title: Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
    Article Snippet: Wildtype chicken avidin (Belovo, Belgium, MW = 16 kDa/monomer) and fibronectin (gelatin-affinity purified from human serum, MW = 220 kDa/monomer) had been previously labeled with Alexa Fluor® 488 NHS ester (Cat. No. A-20000, Thermo Fisher Scientific, Inc. Waltham, MA, USA) by Dr. J. Pärssinen according to manufacturer’s instructions. .. Chemically competent E. coli Top10 cells were acquired from Thermo Fisher scientific, Inc. For biofunctionalization, neutral chimeric avidin (nChiAvd ) and biotinylated alkaline phosphatase (Cat. No. B-2005, Vector Laboratories (Burlingame, CA, USA) were used. .. Vectashield Hardset Antifade Mounting Medium (Cat. No. H-1400, Vector Laboratories) was used for microscope sample preparation.

    Article Title: Induction of cytokines and anti-cytokine autoantibodies in cerebrospinal fluid (CSF) during experimental bacterial meningitis
    Article Snippet: .. After another 1 h of incubation at 37°C and 10 washes, 100 μl of avidin-biotin alkaline phosphatase complex (ABC-AP; Vector Labs, Burlingame, CA) diluted 1:100 in PBS were added for 30 min. Unbound ABC-AP was removed by five consecutive washings with PBS, and 100 μl/well of freshly prepared enzyme substrate solution were added. .. Absorbance was measured after 15 min incubation in the dark in a 405 Multiscan photometer (mcc/340; Labsystem, Helsinki, Finland).

    Article Title: A New Cell Enzyme-Linked Immunosorbent Assay Demonstrates Gamma Interferon Suppression by Beta Interferon in Multiple Sclerosis
    Article Snippet: To detect any captured IFN-γ, 100 μl of biotinylated anti-IFN-γ (7B-B6-1) MAb (0.5 μg/ml; Mabtech) diluted in PBS containing 0.5% Tween 20 and 2% bovine serum albumin was added to the wells. .. After another 60 min of incubation at 37°C and five washes, 100 μl of avidin-biotin alkaline phosphatase complex (Vector Laboratories, Burlingame, Calif.) diluted 1:100 in PBS was added for 45 min. Unbound avidin-biotin alkaline phosphatase complex was removed by five consecutive washings with Tween-PBS, and 100 μl of freshly prepared enzyme substrate solution was added to each well. .. Absorbance was measured after 15 min of incubation in the dark in a 405 Multiscan photometer (mcc/340; Labsystem, Helsinki, Finland).

    Article Title: HIV Nef Protein Causes Endothelial Dysfunction in Porcine Pulmonary Arteries and Human Pulmonary Artery Endothelial Cells
    Article Snippet: .. After washing with PBS, the sections were incubated with biotinylated secondary antibody for 30 min. An avidin-biotin reaction using peroxidase enzyme was used for protein detection (ABC kit; Vector Laboratories, Burlingham, CA). .. Immune complexes were detected with diaminobenzidine under the microscope before counterstaining with hematoxylin for 2 min.

    Article Title: Contrasting effects of myeloid dendritic cells transduced with an adenoviral vector encoding interleukin-10 on organ allograft and tumour rejection
    Article Snippet: Two days later, LacZ expression was quantified by X-gal staining to determine transduction efficiency. .. The efficiency of IL-10 gene transduction was determined on glass slides by immunocytochemical staining for the gene product, using anti-mouse IL-10 monoclonal antibody (mAb; clone JES5–16E3; PharMingen, San Diego, CA), followed by biotinylated polyclonal anti-rat immunoglobulin (1 : 100; PharMingen) and avidin–biotin complex–alkaline phosphatase (ABC–AP; Vector, Burlingame, CA). .. Production of IL-10 by the transduced DC was determined by enzyme-linked immunosorbent assay (ELISA) measurement of mouse IL-10 in the supernatants of 106 DC cultured for 24-hr periods from 0, 24 and 48 hr after gene transfer.

    Transduction:

    Article Title: Contrasting effects of myeloid dendritic cells transduced with an adenoviral vector encoding interleukin-10 on organ allograft and tumour rejection
    Article Snippet: Two days later, LacZ expression was quantified by X-gal staining to determine transduction efficiency. .. The efficiency of IL-10 gene transduction was determined on glass slides by immunocytochemical staining for the gene product, using anti-mouse IL-10 monoclonal antibody (mAb; clone JES5–16E3; PharMingen, San Diego, CA), followed by biotinylated polyclonal anti-rat immunoglobulin (1 : 100; PharMingen) and avidin–biotin complex–alkaline phosphatase (ABC–AP; Vector, Burlingame, CA). .. Production of IL-10 by the transduced DC was determined by enzyme-linked immunosorbent assay (ELISA) measurement of mouse IL-10 in the supernatants of 106 DC cultured for 24-hr periods from 0, 24 and 48 hr after gene transfer.

    Staining:

    Article Title: Contrasting effects of myeloid dendritic cells transduced with an adenoviral vector encoding interleukin-10 on organ allograft and tumour rejection
    Article Snippet: Two days later, LacZ expression was quantified by X-gal staining to determine transduction efficiency. .. The efficiency of IL-10 gene transduction was determined on glass slides by immunocytochemical staining for the gene product, using anti-mouse IL-10 monoclonal antibody (mAb; clone JES5–16E3; PharMingen, San Diego, CA), followed by biotinylated polyclonal anti-rat immunoglobulin (1 : 100; PharMingen) and avidin–biotin complex–alkaline phosphatase (ABC–AP; Vector, Burlingame, CA). .. Production of IL-10 by the transduced DC was determined by enzyme-linked immunosorbent assay (ELISA) measurement of mouse IL-10 in the supernatants of 106 DC cultured for 24-hr periods from 0, 24 and 48 hr after gene transfer.

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    Vector Laboratories avidin biotin complex solution
    Avidin Biotin Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin complex solution/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
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    99/100 stars
      Buy from Supplier

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