avidin biotin complex horseradish peroxidase solution  (Vector Laboratories)


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    Avidin D Peroxidase labeled Av HRP Ready to Use R T U
    Description:
    Horseradish Peroxidase Avidin ready to use reagents are powerful tools to detect or purify biotinylated proteins nucleic acids and other macromolecules Vector Laboratories enzyme conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios Specific covalent linkages are chosen to provide stable highly active conjugates Horseradish Peroxidase Avidin D HRP Avidin D is produced by our own coupling procedure which preserves the high specific activity of the peroxidase Using biotinylated primary or secondary intermediates and peroxidase labeled Avidin D antigens can be localized in histological sections cytospin preparations or smears Horseradish Peroxidase Avidin D is offered as a concentrate 5 mg ml or in a ready to use R T U stabilized solution 100ml 1 g ml in a bottle fitted with a drop dispenser top
    Catalog Number:
    a-2704
    Price:
    None
    Size:
    100 ml
    Category:
    Proteins
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    Structured Review

    Vector Laboratories avidin biotin complex horseradish peroxidase solution
    Avidin D Peroxidase labeled Av HRP Ready to Use R T U
    Horseradish Peroxidase Avidin ready to use reagents are powerful tools to detect or purify biotinylated proteins nucleic acids and other macromolecules Vector Laboratories enzyme conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios Specific covalent linkages are chosen to provide stable highly active conjugates Horseradish Peroxidase Avidin D HRP Avidin D is produced by our own coupling procedure which preserves the high specific activity of the peroxidase Using biotinylated primary or secondary intermediates and peroxidase labeled Avidin D antigens can be localized in histological sections cytospin preparations or smears Horseradish Peroxidase Avidin D is offered as a concentrate 5 mg ml or in a ready to use R T U stabilized solution 100ml 1 g ml in a bottle fitted with a drop dispenser top
    https://www.bioz.com/result/avidin biotin complex horseradish peroxidase solution/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex horseradish peroxidase solution - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Electron Microscopy:

    Article Title: Left Ventricular Hypertrophy is Prevalent in Sprague-Dawley Rats
    Article Snippet: .. Slide-mounted paraffin-embedded tissues were dewaxed (Histoclear, Electron Microscopy Sciences, Hatfield, PA; 3 times for 5 min each), rehydrated through repeated serial-alcohol rinses (100%, 95%, 70%, 0% twice each for 5 min), boiled for 5 min in 10 mM sodium citrate buffer (pH 6.0), cooled, rinsed in 10 mM PBS, incubated in PBS containing 5% normal goat serum (60 min), incubated against rabbit monoclonal antiHDAC3 antibody (1:250, 18 h at 4 °C; catalog no. 04-230, Millipore, Billerica, MA), and detected with a goat antirabbit biotin-conjugated secondary antibody (1:150, 60 min; catalog no. BA-1000, Vector Laboratories, Burlingame, CA) and avidin–peroxidase (Vectastain Elite, Vector Laboratories) before being dehydrated through graded alcohols and coverslipped. .. Myocyte-specific overexpression of HDAC3 has been associated with increased cardiomyocyte hyperplasia without hypertrophy.

    Incubation:

    Article Title: p75NTR Mediates Neurotrophin-Induced Apoptosis of Vascular Smooth Muscle Cells
    Article Snippet: .. Following incubation with the appropriate biotinylated secondary antibody (Vector Laboratories, Burlingame, CA), immunoreactive proteins were detected using an avidin-biotin-based horseradish peroxidase system using Vector VIP solution as a chromogenic substrate (Vector Laboratories), followed by counterstain with hematoxylin. .. Cryopreserved sections of human endarterectomy were air-dried onto microscope slides and postfixed with 3% paraformaldehyde for 10 minutes, followed by incubation with 0.1% sodium citrate containing 0.1% Triton at 4°C.

    Article Title: In vivo localization of human acetylcholinesterase-derived species in a β-sheet conformation at the core of senile plaques in Alzheimer's disease
    Article Snippet: .. Single-labeled sections were incubated with primary antibody (30 min), M.O.M. anti-mouse, or Vectastain anti-rabbit biotinylated antisera (10 min), followed by avidin conjugated to peroxidase (5 min) and DAB as chromagen (2 min, Vector Laboratories), or avidin-FITC conjugate, or Texas red (5 min). .. Double-labeled sections were incubated with first antibody as above.

    Article Title: Dyrk1A Haploinsufficiency Affects Viability and Causes Developmental Delay and Abnormal Brain Morphology in Mice
    Article Snippet: .. Sections were incubated with a biotinylated secondary antibody (dilution, 1:200; Vector Labs) for an hour, and positive signals were developed with a diaminobenzidine substrate by using the avidin-biotin-peroxidase system according to the manufacturer's instructions (Vector Labs). .. Whole-mount in situ hybridization was performed essentially as previously described ( ).

    Article Title: The Substructure of the Suprachiasmatic Nucleus: Similarities between Nocturnal and Diurnal Spiny Mice
    Article Snippet: .. Tissue was then incubated in (iii) biotinylated secondary antibody, followed by (iv) avidin–biotin peroxidase complex (ABC Vectastain Kit; Vector Laboratories, Burlingame, Calif., USA). .. Protein was visualized by reacting with diaminobenzidine (0.5 mg/ml; Sigma) in Trizma buffer (Sigma).

    Article Title: Striking Similarity of Murine Nectin-1? to Human Nectin-1? (HveC) in Sequence and Activity as a Glycoprotein D Receptor for Alphaherpesvirus Entry
    Article Snippet: .. After 1 h, the sections were washed in PBS and incubated with biotinylated goat anti-rabbit IgG for 30 min. After further washes with PBS, the sections were incubated with an avidin-biotinylated horseradish peroxidase complex for 1 h. The sections were washed in PBS and developed by the addition of Vector VIP substrate for peroxidase (Vector Laboratories) as specified by the manufacturer. .. The sequence for murine nectin-1α from FVB/N mice has been deposited in the GenBank database under accession no. .

    Article Title: Left Ventricular Hypertrophy is Prevalent in Sprague-Dawley Rats
    Article Snippet: .. Slide-mounted paraffin-embedded tissues were dewaxed (Histoclear, Electron Microscopy Sciences, Hatfield, PA; 3 times for 5 min each), rehydrated through repeated serial-alcohol rinses (100%, 95%, 70%, 0% twice each for 5 min), boiled for 5 min in 10 mM sodium citrate buffer (pH 6.0), cooled, rinsed in 10 mM PBS, incubated in PBS containing 5% normal goat serum (60 min), incubated against rabbit monoclonal antiHDAC3 antibody (1:250, 18 h at 4 °C; catalog no. 04-230, Millipore, Billerica, MA), and detected with a goat antirabbit biotin-conjugated secondary antibody (1:150, 60 min; catalog no. BA-1000, Vector Laboratories, Burlingame, CA) and avidin–peroxidase (Vectastain Elite, Vector Laboratories) before being dehydrated through graded alcohols and coverslipped. .. Myocyte-specific overexpression of HDAC3 has been associated with increased cardiomyocyte hyperplasia without hypertrophy.

    Binding Assay:

    Article Title: Pancreatic Expression of Keratinocyte Growth Factor Leads to Differentiation of Islet Hepatocytes and Proliferation of Duct Cells
    Article Snippet: .. Binding of the primary antibody was detected using the appropriate secondary antibody (Vector Laboratories, Burlingame, CA) and the horseradish peroxidase (HRP)-labeled avidin-biotin complex (ABC kit, Vector Laboratories). ..

    Avidin-Biotin Assay:

    Article Title: p75NTR Mediates Neurotrophin-Induced Apoptosis of Vascular Smooth Muscle Cells
    Article Snippet: .. Following incubation with the appropriate biotinylated secondary antibody (Vector Laboratories, Burlingame, CA), immunoreactive proteins were detected using an avidin-biotin-based horseradish peroxidase system using Vector VIP solution as a chromogenic substrate (Vector Laboratories), followed by counterstain with hematoxylin. .. Cryopreserved sections of human endarterectomy were air-dried onto microscope slides and postfixed with 3% paraformaldehyde for 10 minutes, followed by incubation with 0.1% sodium citrate containing 0.1% Triton at 4°C.

    Article Title: In vivo localization of human acetylcholinesterase-derived species in a β-sheet conformation at the core of senile plaques in Alzheimer's disease
    Article Snippet: .. Single-labeled sections were incubated with primary antibody (30 min), M.O.M. anti-mouse, or Vectastain anti-rabbit biotinylated antisera (10 min), followed by avidin conjugated to peroxidase (5 min) and DAB as chromagen (2 min, Vector Laboratories), or avidin-FITC conjugate, or Texas red (5 min). .. Double-labeled sections were incubated with first antibody as above.

    Article Title: Testosterone Supplementation Reverses Sarcopenia in Aging through Regulation of Myostatin, c-Jun NH2-Terminal Kinase, Notch, and Akt Signaling Pathways
    Article Snippet: .. Immunoreactivity was detected using biotinylated antirabbit or antimouse IgG secondary antibody followed by avidin-biotinylated horseradish peroxidase complex and visualized with diaminobenzidine tetrahydrochloride as per the manufacturer’s instructions (VECTASTAIN Elile ABC rabbit or mouse IgG kit, Vector Laboratories, Burlingame, CA). .. Negative control was run for every assay and was processed in an identical manner, except the primary antibody was substituted by the rabbit or mouse IgG.

    Article Title: Dyrk1A Haploinsufficiency Affects Viability and Causes Developmental Delay and Abnormal Brain Morphology in Mice
    Article Snippet: .. Sections were incubated with a biotinylated secondary antibody (dilution, 1:200; Vector Labs) for an hour, and positive signals were developed with a diaminobenzidine substrate by using the avidin-biotin-peroxidase system according to the manufacturer's instructions (Vector Labs). .. Whole-mount in situ hybridization was performed essentially as previously described ( ).

    Article Title: The Substructure of the Suprachiasmatic Nucleus: Similarities between Nocturnal and Diurnal Spiny Mice
    Article Snippet: .. Tissue was then incubated in (iii) biotinylated secondary antibody, followed by (iv) avidin–biotin peroxidase complex (ABC Vectastain Kit; Vector Laboratories, Burlingame, Calif., USA). .. Protein was visualized by reacting with diaminobenzidine (0.5 mg/ml; Sigma) in Trizma buffer (Sigma).

    Article Title: Striking Similarity of Murine Nectin-1? to Human Nectin-1? (HveC) in Sequence and Activity as a Glycoprotein D Receptor for Alphaherpesvirus Entry
    Article Snippet: .. After 1 h, the sections were washed in PBS and incubated with biotinylated goat anti-rabbit IgG for 30 min. After further washes with PBS, the sections were incubated with an avidin-biotinylated horseradish peroxidase complex for 1 h. The sections were washed in PBS and developed by the addition of Vector VIP substrate for peroxidase (Vector Laboratories) as specified by the manufacturer. .. The sequence for murine nectin-1α from FVB/N mice has been deposited in the GenBank database under accession no. .

    Article Title: Left Ventricular Hypertrophy is Prevalent in Sprague-Dawley Rats
    Article Snippet: .. Slide-mounted paraffin-embedded tissues were dewaxed (Histoclear, Electron Microscopy Sciences, Hatfield, PA; 3 times for 5 min each), rehydrated through repeated serial-alcohol rinses (100%, 95%, 70%, 0% twice each for 5 min), boiled for 5 min in 10 mM sodium citrate buffer (pH 6.0), cooled, rinsed in 10 mM PBS, incubated in PBS containing 5% normal goat serum (60 min), incubated against rabbit monoclonal antiHDAC3 antibody (1:250, 18 h at 4 °C; catalog no. 04-230, Millipore, Billerica, MA), and detected with a goat antirabbit biotin-conjugated secondary antibody (1:150, 60 min; catalog no. BA-1000, Vector Laboratories, Burlingame, CA) and avidin–peroxidase (Vectastain Elite, Vector Laboratories) before being dehydrated through graded alcohols and coverslipped. .. Myocyte-specific overexpression of HDAC3 has been associated with increased cardiomyocyte hyperplasia without hypertrophy.

    Article Title: Pancreatic Expression of Keratinocyte Growth Factor Leads to Differentiation of Islet Hepatocytes and Proliferation of Duct Cells
    Article Snippet: .. Binding of the primary antibody was detected using the appropriate secondary antibody (Vector Laboratories, Burlingame, CA) and the horseradish peroxidase (HRP)-labeled avidin-biotin complex (ABC kit, Vector Laboratories). ..

    Plasmid Preparation:

    Article Title: p75NTR Mediates Neurotrophin-Induced Apoptosis of Vascular Smooth Muscle Cells
    Article Snippet: .. Following incubation with the appropriate biotinylated secondary antibody (Vector Laboratories, Burlingame, CA), immunoreactive proteins were detected using an avidin-biotin-based horseradish peroxidase system using Vector VIP solution as a chromogenic substrate (Vector Laboratories), followed by counterstain with hematoxylin. .. Cryopreserved sections of human endarterectomy were air-dried onto microscope slides and postfixed with 3% paraformaldehyde for 10 minutes, followed by incubation with 0.1% sodium citrate containing 0.1% Triton at 4°C.

    Article Title: Striking Similarity of Murine Nectin-1? to Human Nectin-1? (HveC) in Sequence and Activity as a Glycoprotein D Receptor for Alphaherpesvirus Entry
    Article Snippet: .. After 1 h, the sections were washed in PBS and incubated with biotinylated goat anti-rabbit IgG for 30 min. After further washes with PBS, the sections were incubated with an avidin-biotinylated horseradish peroxidase complex for 1 h. The sections were washed in PBS and developed by the addition of Vector VIP substrate for peroxidase (Vector Laboratories) as specified by the manufacturer. .. The sequence for murine nectin-1α from FVB/N mice has been deposited in the GenBank database under accession no. .

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    Vector Laboratories avidin biotin complex conjugated to hrp
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