avidin biotin complex blotting proteins  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Proteins
    Description:
    This book provides an overview of the various medical diagnostic and industrial uses of proteins It has evolved from an earlier text Protein Biotechnology and includes the latest developments in the field and offers a varied selection of relevant case study material Initial chapters focus upon issues such as protein structure folding stability purification and characterization Later chapters detail the industrial production of proteins and their applications in medicine analysis and industry It presents a comprehensive review of proteins and enzymes used in the diagnostic therapeutic and industrial areas
    Catalog Number:
    p7492
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore avidin biotin complex blotting proteins
    Proteins
    This book provides an overview of the various medical diagnostic and industrial uses of proteins It has evolved from an earlier text Protein Biotechnology and includes the latest developments in the field and offers a varied selection of relevant case study material Initial chapters focus upon issues such as protein structure folding stability purification and characterization Later chapters detail the industrial production of proteins and their applications in medicine analysis and industry It presents a comprehensive review of proteins and enzymes used in the diagnostic therapeutic and industrial areas
    https://www.bioz.com/result/avidin biotin complex blotting proteins/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex blotting proteins - by Bioz Stars, 2020-07
    99/100 stars

    Related Products / Commonly Used Together

    pvdf membranes
    mini trans-blot electrophoretic-transfer cell

    Images

    Related Articles

    In Vitro:

    Article Title: Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions
    Article Snippet: .. In vitro binding assays with GST fusion protein and analysis of cAMP-induced activation of RasB For interaction with cytoskeletal proteins, 5 × 107 AX2 cells were lysed in lysis buffer (LB; 25 mM Tris/HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.5% Triton X-100, and 1 mM DTT, supplemented with protease inhibitors [Sigma-Aldrich] with 5 mM ATP added) with or without a preincubation with 10 μM Lat A (Sigma-Aldrich for 1 h) and incubated with equal amounts of GST-GEF bound to beads for 3 h at 4°C. .. Beads were washed with wash buffer (25 mM Tris/HCl, pH 7.5, 150 mM NaCl, and 5 mM EDTA) and the pulldown eluates were analyzed in Western blots.

    Construct:

    Article Title: Analysis of Novel Iron-Regulated, Surface-Anchored Hemin-Binding Proteins in Corynebacterium diphtheriae
    Article Snippet: .. Plasmid constructs that expressed the N-terminally Strep-tagged proteins ChtA, ChtA CR, ChtA C-terminal region, ChtB, and ChtC were constructed using the pET24a vector (Novagen). .. PCR-derived DNA fragments harboring the gene of interest were initially cloned into the pCR-Blunt II-TOPO vector and subsequently ligated into the NdeI and EcoRI sites of pET24a.

    Purification:

    Article Title: Catabolic Repression of secB Expression Is Positively Controlled by Cyclic AMP (cAMP) Receptor Protein-cAMP Complexes at the Transcriptional Level
    Article Snippet: .. 32 P-labeled DNA fragments and purified CRP proteins were incubated in binding buffer (10 mM Tris-HCl [pH 7.8], 50 mM KCl, 1 mM EDTA, 50 μg of bovine serum albumin/ml, 1 mM DTT, 0.05% Nonidet P-40, 50 μM cAMP, 20 μg of salmon sperm DNA [Sigma Chemical Co., St. Louis, Mo. .. Then, 3 μl of loading buffer (binding buffer containing 50% glycerol and 0.1 mg of bromophenol blue/ml) was added, and the samples were immediately loaded on a 5% polyacrylamide gel (Protogel; National Diagnostics), with current applied.

    Article Title: CK2 phosphorylation of the PRH/Hex homeodomain functions as a reversible switch for DNA binding
    Article Snippet: .. Protein phosphorylation and dephosphorylation Purified PRH protein, truncated PRH proteins and PRH mutants (10 ng) were incubated in CK2 phosphorylation buffer (50 mM KCl, 10 mM MgCl2 , 20 mM Tris–HCl pH 7.5, 200 μM ATP) with 100 U CK2 enzyme (500 U/μl Calbiochem) for 30 min at 30°C. .. Dephosphorylation was brought about by incubation of the phosphorylated protein with 10 U of calf alkaline phosphatase (CAP) (6 U/μl Promega) in CAP buffer (50 mM Tris–HCl pH 9.3, 10 mM MgCl2 , 1 mM ZnCl2 , 10 mM spermidine) for 20 min at 25°C.

    Activation Assay:

    Article Title: Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions
    Article Snippet: .. In vitro binding assays with GST fusion protein and analysis of cAMP-induced activation of RasB For interaction with cytoskeletal proteins, 5 × 107 AX2 cells were lysed in lysis buffer (LB; 25 mM Tris/HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.5% Triton X-100, and 1 mM DTT, supplemented with protease inhibitors [Sigma-Aldrich] with 5 mM ATP added) with or without a preincubation with 10 μM Lat A (Sigma-Aldrich for 1 h) and incubated with equal amounts of GST-GEF bound to beads for 3 h at 4°C. .. Beads were washed with wash buffer (25 mM Tris/HCl, pH 7.5, 150 mM NaCl, and 5 mM EDTA) and the pulldown eluates were analyzed in Western blots.

    Incubation:

    Article Title: Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions
    Article Snippet: .. In vitro binding assays with GST fusion protein and analysis of cAMP-induced activation of RasB For interaction with cytoskeletal proteins, 5 × 107 AX2 cells were lysed in lysis buffer (LB; 25 mM Tris/HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.5% Triton X-100, and 1 mM DTT, supplemented with protease inhibitors [Sigma-Aldrich] with 5 mM ATP added) with or without a preincubation with 10 μM Lat A (Sigma-Aldrich for 1 h) and incubated with equal amounts of GST-GEF bound to beads for 3 h at 4°C. .. Beads were washed with wash buffer (25 mM Tris/HCl, pH 7.5, 150 mM NaCl, and 5 mM EDTA) and the pulldown eluates were analyzed in Western blots.

    Article Title: Development of a Biological Scaffold Engineered Using the Extracellular Matrix Secreted by Skeletal Muscle Cells
    Article Snippet: .. To visualize accumulated ECM proteins within DSM and eECM samples, mounted sections were immune-reacted for the presence of collagen type I (αrat collagen 1, mouse IgG1, 750:1, Sigma, St. Louis MO) and cellular fibronectin (αrat cellular fibronectin, mouse IgM, 400:1, Sigma, St. Louis MO) followed by incubation with the appropriate fluorescently labeled secondary antibodies (500:1, Invitrogen, Carlsbad, CA). .. Sections were counterstained with the nuclear staining reagent DAPI, and then microscopically imaged.

    Article Title: Catabolic Repression of secB Expression Is Positively Controlled by Cyclic AMP (cAMP) Receptor Protein-cAMP Complexes at the Transcriptional Level
    Article Snippet: .. 32 P-labeled DNA fragments and purified CRP proteins were incubated in binding buffer (10 mM Tris-HCl [pH 7.8], 50 mM KCl, 1 mM EDTA, 50 μg of bovine serum albumin/ml, 1 mM DTT, 0.05% Nonidet P-40, 50 μM cAMP, 20 μg of salmon sperm DNA [Sigma Chemical Co., St. Louis, Mo. .. Then, 3 μl of loading buffer (binding buffer containing 50% glycerol and 0.1 mg of bromophenol blue/ml) was added, and the samples were immediately loaded on a 5% polyacrylamide gel (Protogel; National Diagnostics), with current applied.

    Article Title: CK2 phosphorylation of the PRH/Hex homeodomain functions as a reversible switch for DNA binding
    Article Snippet: .. Protein phosphorylation and dephosphorylation Purified PRH protein, truncated PRH proteins and PRH mutants (10 ng) were incubated in CK2 phosphorylation buffer (50 mM KCl, 10 mM MgCl2 , 20 mM Tris–HCl pH 7.5, 200 μM ATP) with 100 U CK2 enzyme (500 U/μl Calbiochem) for 30 min at 30°C. .. Dephosphorylation was brought about by incubation of the phosphorylated protein with 10 U of calf alkaline phosphatase (CAP) (6 U/μl Promega) in CAP buffer (50 mM Tris–HCl pH 9.3, 10 mM MgCl2 , 1 mM ZnCl2 , 10 mM spermidine) for 20 min at 25°C.

    Labeling:

    Article Title: Development of a Biological Scaffold Engineered Using the Extracellular Matrix Secreted by Skeletal Muscle Cells
    Article Snippet: .. To visualize accumulated ECM proteins within DSM and eECM samples, mounted sections were immune-reacted for the presence of collagen type I (αrat collagen 1, mouse IgG1, 750:1, Sigma, St. Louis MO) and cellular fibronectin (αrat cellular fibronectin, mouse IgM, 400:1, Sigma, St. Louis MO) followed by incubation with the appropriate fluorescently labeled secondary antibodies (500:1, Invitrogen, Carlsbad, CA). .. Sections were counterstained with the nuclear staining reagent DAPI, and then microscopically imaged.

    De-Phosphorylation Assay:

    Article Title: CK2 phosphorylation of the PRH/Hex homeodomain functions as a reversible switch for DNA binding
    Article Snippet: .. Protein phosphorylation and dephosphorylation Purified PRH protein, truncated PRH proteins and PRH mutants (10 ng) were incubated in CK2 phosphorylation buffer (50 mM KCl, 10 mM MgCl2 , 20 mM Tris–HCl pH 7.5, 200 μM ATP) with 100 U CK2 enzyme (500 U/μl Calbiochem) for 30 min at 30°C. .. Dephosphorylation was brought about by incubation of the phosphorylated protein with 10 U of calf alkaline phosphatase (CAP) (6 U/μl Promega) in CAP buffer (50 mM Tris–HCl pH 9.3, 10 mM MgCl2 , 1 mM ZnCl2 , 10 mM spermidine) for 20 min at 25°C.

    Staining:

    Article Title: An in silico model of the ubiquitin-proteasome system that incorporates normal homeostasis and age-related decline
    Article Snippet: .. The membranes were autoclaved on a liquid cycle for 45 minutes to enhance the detection of poly-ubiquitinated proteins, stained with Ponceau S (Sigma, St. Louis, Missouri, USA) and analyzed by western blotting with the indicated antibody. .. The proteins were visualized by a horseradish peroxidase method using the ECL kit from Kirkegaard and Perry Laboratories Inc., Gaithersburg, Maryland, USA.

    Western Blot:

    Article Title: An in silico model of the ubiquitin-proteasome system that incorporates normal homeostasis and age-related decline
    Article Snippet: .. The membranes were autoclaved on a liquid cycle for 45 minutes to enhance the detection of poly-ubiquitinated proteins, stained with Ponceau S (Sigma, St. Louis, Missouri, USA) and analyzed by western blotting with the indicated antibody. .. The proteins were visualized by a horseradish peroxidase method using the ECL kit from Kirkegaard and Perry Laboratories Inc., Gaithersburg, Maryland, USA.

    Lysis:

    Article Title: Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions
    Article Snippet: .. In vitro binding assays with GST fusion protein and analysis of cAMP-induced activation of RasB For interaction with cytoskeletal proteins, 5 × 107 AX2 cells were lysed in lysis buffer (LB; 25 mM Tris/HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.5% Triton X-100, and 1 mM DTT, supplemented with protease inhibitors [Sigma-Aldrich] with 5 mM ATP added) with or without a preincubation with 10 μM Lat A (Sigma-Aldrich for 1 h) and incubated with equal amounts of GST-GEF bound to beads for 3 h at 4°C. .. Beads were washed with wash buffer (25 mM Tris/HCl, pH 7.5, 150 mM NaCl, and 5 mM EDTA) and the pulldown eluates were analyzed in Western blots.

    Binding Assay:

    Article Title: Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions
    Article Snippet: .. In vitro binding assays with GST fusion protein and analysis of cAMP-induced activation of RasB For interaction with cytoskeletal proteins, 5 × 107 AX2 cells were lysed in lysis buffer (LB; 25 mM Tris/HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.5% Triton X-100, and 1 mM DTT, supplemented with protease inhibitors [Sigma-Aldrich] with 5 mM ATP added) with or without a preincubation with 10 μM Lat A (Sigma-Aldrich for 1 h) and incubated with equal amounts of GST-GEF bound to beads for 3 h at 4°C. .. Beads were washed with wash buffer (25 mM Tris/HCl, pH 7.5, 150 mM NaCl, and 5 mM EDTA) and the pulldown eluates were analyzed in Western blots.

    Article Title: Catabolic Repression of secB Expression Is Positively Controlled by Cyclic AMP (cAMP) Receptor Protein-cAMP Complexes at the Transcriptional Level
    Article Snippet: .. 32 P-labeled DNA fragments and purified CRP proteins were incubated in binding buffer (10 mM Tris-HCl [pH 7.8], 50 mM KCl, 1 mM EDTA, 50 μg of bovine serum albumin/ml, 1 mM DTT, 0.05% Nonidet P-40, 50 μM cAMP, 20 μg of salmon sperm DNA [Sigma Chemical Co., St. Louis, Mo. .. Then, 3 μl of loading buffer (binding buffer containing 50% glycerol and 0.1 mg of bromophenol blue/ml) was added, and the samples were immediately loaded on a 5% polyacrylamide gel (Protogel; National Diagnostics), with current applied.

    SDS Page:

    Article Title: The phosphoinositide phosphatase Sac1 regulates cell shape and microtubule stability in the developing Drosophila eye
    Article Snippet: .. Flag-tagged Sac1 proteins were analyzed by SDS page and immunoblotting (1:1000) using mouse monoclonal anti-Flag M2 antiserum (Sigma-Aldrich F3165). .. Anti-GAPDH antibody (1:1000; Abcam, ab9485) was used as the loading control.

    Plasmid Preparation:

    Article Title: Analysis of Novel Iron-Regulated, Surface-Anchored Hemin-Binding Proteins in Corynebacterium diphtheriae
    Article Snippet: .. Plasmid constructs that expressed the N-terminally Strep-tagged proteins ChtA, ChtA CR, ChtA C-terminal region, ChtB, and ChtC were constructed using the pET24a vector (Novagen). .. PCR-derived DNA fragments harboring the gene of interest were initially cloned into the pCR-Blunt II-TOPO vector and subsequently ligated into the NdeI and EcoRI sites of pET24a.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore avidin biotin complex blotting proteins
    Avidin Biotin Complex Blotting Proteins, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin complex blotting proteins/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex blotting proteins - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results