avidin biotin complex abc solution  (Vector Laboratories)


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    Name:
    VECTASTAIN ABC HRP Kit Peroxidase Standard
    Description:
    VECTASTAIN ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes Original VECTASTAIN ABC Kit Good sensitivity low backgroundOriginal avidin biotin ABC complex formulationLow costAvailable without Standard kit or with biotinylated species specific secondary antibody Kit Contents 2 ml Reagent A2 ml Reagent BReagents A and B form the ABC enzyme complex The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    Catalog Number:
    pk-4000
    Price:
    None
    Size:
    1 Kit
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    No antibody included
    Buy from Supplier


    Structured Review

    Vector Laboratories avidin biotin complex abc solution
    VECTASTAIN ABC HRP Kit Peroxidase Standard
    VECTASTAIN ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes Original VECTASTAIN ABC Kit Good sensitivity low backgroundOriginal avidin biotin ABC complex formulationLow costAvailable without Standard kit or with biotinylated species specific secondary antibody Kit Contents 2 ml Reagent A2 ml Reagent BReagents A and B form the ABC enzyme complex The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    https://www.bioz.com/result/avidin biotin complex abc solution/product/Vector Laboratories
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex abc solution - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: A Possible Contribution of Altered Cathepsin B Expression to the Development of Skin Sclerosis and Vasculopathy in Systemic Sclerosis
    Article Snippet: .. Immunohistochemistry Immunohistochemistry with Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA) was performed on formalin-fixed, paraffin-embedded tissue sections using anti-human CTSB antibody (R & D Systems) or anti-mouse CTSB antibody (Santa Cruz, Santa Cruz, CA, USA). .. Skin samples were obtained from forearms of 8 SSc patients and 8 closely matched healthy controls and from the back of 3-month-old mice.

    Avidin-Biotin Assay:

    Article Title: Heparan Sulfate Proteoglycan Is an Important Attachment Factor for Cell Entry of Akabane and Schmallenberg Viruses
    Article Snippet: .. After washing with PBS-T, avidin-biotinylated horseradish peroxidase (HRP) complex (Vectastain ABC kit; Vector Laboratories) was added to the wells and incubated for 30 min at room temperature. ..

    Article Title: Green algae Chlamydomonas reinhardtii possess endogenous sialylated N-glycans
    Article Snippet: .. The membrane was washed three times with Tris-buffered saline (TBS) (50 mM Tris–HCl, pH 7.5, 150 mM NaCl) and blocked with Carbo-free blocking buffer (Cat. No. SP-5040, Vector Laboratories, Burlingame, CA) for 1 h. After blocking, the membrane was incubated in the lectin incubation buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM CaCl2 , 1 mM MgCl2 , 1 mM MnCl2 ) containing 10 μg/ml biotinylated SNA-1 (Sambucus nigra , Cat. No. BA-6802-1, EY Laboratories, San Mateo, CA) and 50 μg/ml MAA (Maackia amurensis , Cat. No. BA-7801-5, EY Laboratories) for 2 h. After three washes with TBS, the membrane was incubated with avidin plus biotinylated horseradish peroxidase (HRP) using ABC kit (Cat. No. PK-4000, Vector Laboratories) for 30 min and washed three times with TBS. .. Lectin binding to sialic acid-containing proteins was visualized using SuperSignal West Pico Chemiluminescent Substrate (Pierce, Rockford, IL).

    Immunostaining:

    Article Title: Exploitation of Nucleic Acid Packaging Signals To Generate a Novel Influenza Virus-Based Vector Stably Expressing Two Foreign Genes
    Article Snippet: .. Bound antibodies were detected with a Vectastain ABC kit (Vector, Burlingame, Calif.) and a Konica immunostaining kit. .. vRNA present in 293T cells transfected with PolI plasmids was extracted with the Isogen RNA extraction kit (Nippon Gene, Tokyo, Japan) at 24 h posttransfection.

    Incubation:

    Article Title: Heparan Sulfate Proteoglycan Is an Important Attachment Factor for Cell Entry of Akabane and Schmallenberg Viruses
    Article Snippet: .. After washing with PBS-T, avidin-biotinylated horseradish peroxidase (HRP) complex (Vectastain ABC kit; Vector Laboratories) was added to the wells and incubated for 30 min at room temperature. ..

    Article Title: Green algae Chlamydomonas reinhardtii possess endogenous sialylated N-glycans
    Article Snippet: .. The membrane was washed three times with Tris-buffered saline (TBS) (50 mM Tris–HCl, pH 7.5, 150 mM NaCl) and blocked with Carbo-free blocking buffer (Cat. No. SP-5040, Vector Laboratories, Burlingame, CA) for 1 h. After blocking, the membrane was incubated in the lectin incubation buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM CaCl2 , 1 mM MgCl2 , 1 mM MnCl2 ) containing 10 μg/ml biotinylated SNA-1 (Sambucus nigra , Cat. No. BA-6802-1, EY Laboratories, San Mateo, CA) and 50 μg/ml MAA (Maackia amurensis , Cat. No. BA-7801-5, EY Laboratories) for 2 h. After three washes with TBS, the membrane was incubated with avidin plus biotinylated horseradish peroxidase (HRP) using ABC kit (Cat. No. PK-4000, Vector Laboratories) for 30 min and washed three times with TBS. .. Lectin binding to sialic acid-containing proteins was visualized using SuperSignal West Pico Chemiluminescent Substrate (Pierce, Rockford, IL).

    Formalin-fixed Paraffin-Embedded:

    Article Title: A Possible Contribution of Altered Cathepsin B Expression to the Development of Skin Sclerosis and Vasculopathy in Systemic Sclerosis
    Article Snippet: .. Immunohistochemistry Immunohistochemistry with Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA) was performed on formalin-fixed, paraffin-embedded tissue sections using anti-human CTSB antibody (R & D Systems) or anti-mouse CTSB antibody (Santa Cruz, Santa Cruz, CA, USA). .. Skin samples were obtained from forearms of 8 SSc patients and 8 closely matched healthy controls and from the back of 3-month-old mice.

    Blocking Assay:

    Article Title: Green algae Chlamydomonas reinhardtii possess endogenous sialylated N-glycans
    Article Snippet: .. The membrane was washed three times with Tris-buffered saline (TBS) (50 mM Tris–HCl, pH 7.5, 150 mM NaCl) and blocked with Carbo-free blocking buffer (Cat. No. SP-5040, Vector Laboratories, Burlingame, CA) for 1 h. After blocking, the membrane was incubated in the lectin incubation buffer (50 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM CaCl2 , 1 mM MgCl2 , 1 mM MnCl2 ) containing 10 μg/ml biotinylated SNA-1 (Sambucus nigra , Cat. No. BA-6802-1, EY Laboratories, San Mateo, CA) and 50 μg/ml MAA (Maackia amurensis , Cat. No. BA-7801-5, EY Laboratories) for 2 h. After three washes with TBS, the membrane was incubated with avidin plus biotinylated horseradish peroxidase (HRP) using ABC kit (Cat. No. PK-4000, Vector Laboratories) for 30 min and washed three times with TBS. .. Lectin binding to sialic acid-containing proteins was visualized using SuperSignal West Pico Chemiluminescent Substrate (Pierce, Rockford, IL).

    Staining:

    Article Title: Progesterone Synthesized by Schwann Cells during Myelin Formation Regulates Neuronal Gene Expression
    Article Snippet: .. Coverslips were washed and stained using the horseradish peroxidase conjugated Vectastain ABC staining kit (Vector Laboratories) and the ImmunoPure Metal Enhanced DAB Substrate (Pierce). ..

    Plasmid Preparation:

    Article Title: Tissue Distibution of Murine Muc19/Smgc Gene Products
    Article Snippet: .. Sections were then probed with rabbit anti-Muc19 or preimmune serum (1:300 dilution) as described previously , except that we used a Vectastain ABC-HRP kit with Vector Red substrate (Vector Laboratories; Burlingame, CA), and counterstained with hematoxylin. ..

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  • 88
    Vector Laboratories avidin biotin complex abc solution
    Avidin Biotin Complex Abc Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 88/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin complex abc solution/product/Vector Laboratories
    Average 88 stars, based on 207 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex abc solution - by Bioz Stars, 2020-09
    88/100 stars
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