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Abcam avidin biotin blocking
Avidin Biotin Blocking, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/avidin biotin blocking/product/Abcam
Average 90 stars, based on 3 article reviews
Price from $9.99 to $1999.99
avidin biotin blocking - by Bioz Stars, 2020-09
90/100 stars

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Related Articles

Avidin-Biotin Assay:

Article Title: Pre-conditioned mesenchymal stem cells ameliorate renal ischemic injury in rats by augmented survival and engraftment
Article Snippet: .. Briefly, the sections were subjected to citrate based antigen retrieval followed by endogenous peroxidase and avidin-biotin blocking using a Streptavidin-Biotin blocking reagent (Abcam, UK). .. Following serum blocking, sections were incubated either for one hour at room temperature or overnight at 4°C in humid conditions with the following primary antibodies of specified dilutions: a Rabbit polyclonal anti iNOS antibody, mouse monoclonal to VEGF, rabbit polyclonal to Ki-67, rabbit polyclonal anti eNOS antibody (Abcam, UK), rabbit polyclonal to IGF1 and rabbit polyclonal Bcl-2 (Santa Cruz, USA) followed by HRP conjugated secondary antibodies.

Article Title: Notch ligand Dll4 impairs cell recruitment into aortic clusters and limits hematopoietic stem cells
Article Snippet: .. For CD31 staining, an additional blocking of endogenous biotin was performed (Endogenous Avidin+Biotin Blocking System, ab3387 abcam) and detected with streptavidin Alexa Red 555 (BD 32355). .. For BrdU and Ki67 staining a primary antigen retrieval was performed in 10 mM sodium citrate pH6, 30 min at 80°C.

Article Title: NKT cells are important mediators of hepatic ischemia-reperfusion injury
Article Snippet: .. Sections were deparaffinised and rehydrated before endogenous peroxidase and avidin/biotin activity were quenched, prior to incubating with a rat monoclonal anti-mouse Ly6g antibody (Ab25377, Abcam, Cambridge, UK) at a dilution of 1 in 100. .. Slides were subsequently incubated at room temperature with polyclonal rabbit anti-rat biotinylated secondary antibody (E0468, DAKO, Ely, UK) at 1 in 400 dilution for 40 min.

Article Title: Expression of semaphorin 6D and its receptor plexin-A1 in gastric cancer and their association with tumor angiogenesis
Article Snippet: .. The sections were blocked for endogenous avidin, peroxidase and biotin, and then incubated with anti-plexin-A1 antibody (ab32960; Abcam) or anti-Sema6D antibody (sc-67965; Santa Cruz Biotechnology, Inc.) overnight at 4°C. .. Upon washing three times with phosphate-buffered saline (PBS), the staining was developed using the Universal LSAB™ kit/HRP, Rabbit/Mouse/Goat (Dako, Glostrup, Denmark) according to the manufacturer's protocol.

Article Title: Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells
Article Snippet: .. The samples were treated with Endogenous Avidin/Biotin Blocking System (Abcam) and were subsequently blocked in PBS containing 5.0% BSA and 0.1% Triton. .. The samples were incubated with our anti-KSP antibody conjugated with biotin, anti-human specific mitochondria antibody conjugated with biotin (Abcam), anti-Megalin antibody, anti-AQP2 (Alomone Labs) or anti-Podocalyxin (R & D) antibodies.

Blocking Assay:

Article Title: Pre-conditioned mesenchymal stem cells ameliorate renal ischemic injury in rats by augmented survival and engraftment
Article Snippet: .. Briefly, the sections were subjected to citrate based antigen retrieval followed by endogenous peroxidase and avidin-biotin blocking using a Streptavidin-Biotin blocking reagent (Abcam, UK). .. Following serum blocking, sections were incubated either for one hour at room temperature or overnight at 4°C in humid conditions with the following primary antibodies of specified dilutions: a Rabbit polyclonal anti iNOS antibody, mouse monoclonal to VEGF, rabbit polyclonal to Ki-67, rabbit polyclonal anti eNOS antibody (Abcam, UK), rabbit polyclonal to IGF1 and rabbit polyclonal Bcl-2 (Santa Cruz, USA) followed by HRP conjugated secondary antibodies.

Article Title: Notch ligand Dll4 impairs cell recruitment into aortic clusters and limits hematopoietic stem cells
Article Snippet: .. For CD31 staining, an additional blocking of endogenous biotin was performed (Endogenous Avidin+Biotin Blocking System, ab3387 abcam) and detected with streptavidin Alexa Red 555 (BD 32355). .. For BrdU and Ki67 staining a primary antigen retrieval was performed in 10 mM sodium citrate pH6, 30 min at 80°C.

Article Title: Protease-Activated Receptor-2 Regulates Neuro-Epidermal Communication in Atopic Dermatitis
Article Snippet: .. For Cryosections Five-micrometer sections of frozen samples were fixed with methanol, followed by inactivation of endogenous peroxidase with 0.3% H2 O2, blocking of endogenous biotin with Biotin-Blocking System (DAKO) and unspecific binding with 5% rabbit serum. .. The samples were incubated with the respective primary antibody, followed by incubation with the relevant horseradish peroxidase-labeled secondary antibody (Vector).

Article Title: Kidney Specific Protein-Positive Cells Derived from Embryonic Stem Cells Reproduce Tubular Structures In Vitro and Differentiate into Renal Tubular Cells
Article Snippet: .. The samples were treated with Endogenous Avidin/Biotin Blocking System (Abcam) and were subsequently blocked in PBS containing 5.0% BSA and 0.1% Triton. .. The samples were incubated with our anti-KSP antibody conjugated with biotin, anti-human specific mitochondria antibody conjugated with biotin (Abcam), anti-Megalin antibody, anti-AQP2 (Alomone Labs) or anti-Podocalyxin (R & D) antibodies.

Incubation:

Article Title: Expression of semaphorin 6D and its receptor plexin-A1 in gastric cancer and their association with tumor angiogenesis
Article Snippet: .. The sections were blocked for endogenous avidin, peroxidase and biotin, and then incubated with anti-plexin-A1 antibody (ab32960; Abcam) or anti-Sema6D antibody (sc-67965; Santa Cruz Biotechnology, Inc.) overnight at 4°C. .. Upon washing three times with phosphate-buffered saline (PBS), the staining was developed using the Universal LSAB™ kit/HRP, Rabbit/Mouse/Goat (Dako, Glostrup, Denmark) according to the manufacturer's protocol.

other:

Article Title: Slow-wave coupling across a gastroduodenal anastomosis as a mechanism for postsurgical gastric dysfunction: evidence for a “gastrointestinal aberrant pathway”
Article Snippet: After treatment to neutralize endogenous biotin and peroxidases, the primary antibody, mouse IgG2b against human DOG-1 (also known as ANO1; Leica, Buffalo Grove, IL) was added at 3–30 µg/ml diluted in Tris-buffered saline overnight at 4°C.

Activity Assay:

Article Title: NKT cells are important mediators of hepatic ischemia-reperfusion injury
Article Snippet: .. Sections were deparaffinised and rehydrated before endogenous peroxidase and avidin/biotin activity were quenched, prior to incubating with a rat monoclonal anti-mouse Ly6g antibody (Ab25377, Abcam, Cambridge, UK) at a dilution of 1 in 100. .. Slides were subsequently incubated at room temperature with polyclonal rabbit anti-rat biotinylated secondary antibody (E0468, DAKO, Ely, UK) at 1 in 400 dilution for 40 min.

Staining:

Article Title: Notch ligand Dll4 impairs cell recruitment into aortic clusters and limits hematopoietic stem cells
Article Snippet: .. For CD31 staining, an additional blocking of endogenous biotin was performed (Endogenous Avidin+Biotin Blocking System, ab3387 abcam) and detected with streptavidin Alexa Red 555 (BD 32355). .. For BrdU and Ki67 staining a primary antigen retrieval was performed in 10 mM sodium citrate pH6, 30 min at 80°C.

Binding Assay:

Article Title: Protease-Activated Receptor-2 Regulates Neuro-Epidermal Communication in Atopic Dermatitis
Article Snippet: .. For Cryosections Five-micrometer sections of frozen samples were fixed with methanol, followed by inactivation of endogenous peroxidase with 0.3% H2 O2, blocking of endogenous biotin with Biotin-Blocking System (DAKO) and unspecific binding with 5% rabbit serum. .. The samples were incubated with the respective primary antibody, followed by incubation with the relevant horseradish peroxidase-labeled secondary antibody (Vector).

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    Abcam avidin biotin blocking kit
    Avidin Biotin Blocking Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin blocking kit/product/Abcam
    Average 95 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    avidin biotin blocking kit - by Bioz Stars, 2020-09
    95/100 stars
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