avidin biotin antibody complex  (Millipore)


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  • 90
    Name:
    Anti Avidin Biotin antibody
    Description:
    The antibody binds to the avidin molecule without interfering with the biotin binding reaction The product reacts specifically with egg white avidin ExtrAvidin and their complexes formed with biotin The antibody shows no cross reaction with streptavidin
    Catalog Number:
    b9655
    Price:
    None
    Applications:
    Anti-Avidin-Biotin antibody, Mouse monoclonal may be used for ELISA at a working dilution of 1:150,000; for dot blot at a working dilution of 1:20,000; for immunohistochemistry at a working dilution of 1:2000. It was used for FISH analysis of chromosomes X and 8 in lymphocytes isolated from women.
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    Structured Review

    Millipore avidin biotin antibody complex
    The antibody binds to the avidin molecule without interfering with the biotin binding reaction The product reacts specifically with egg white avidin ExtrAvidin and their complexes formed with biotin The antibody shows no cross reaction with streptavidin
    https://www.bioz.com/result/avidin biotin antibody complex/product/Millipore
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin antibody complex - by Bioz Stars, 2020-09
    90/100 stars

    Related Products / Commonly Used Together

    nickel-intensified diaminobenzidine reaction

    Images

    Related Articles

    Amplification:

    Article Title: Basic cytogenetics and physical mapping of 5S and 18S ribosomal genes in Hoplias malabaricus (Osteichthyes, Characiformes, Erythrinidae) from isolated natural lagoons: a conserved karyomorph along the Iguaçu river basin
    Article Snippet: .. The detection and amplification of the hybridization signal was performed using antidigoxigenin-rhodamine for 5S rDNA (Roche) and avidin-FITC and anti-avidin-biotin for 18S rDNA (Sigma). .. The best metaphases were captured in an Olympus BX60 photomicroscope with a digital camera DP71 and DPcontroller 3.2.1.276 software (Olympus).

    Article Title: Extra Microchromosomes Play Male Determination Role in Polyploid Gibel Carp
    Article Snippet: .. The probe with Biotin was first stained with ExtraAvidin-Cy3 antibody (Sigma Chemical, St. Louis, MO) and the signal was amplified by orderly adding monoclonal Biotin-conjugated anti-avidin antibody (Sigma Chemical) and ExtraAvidin-Cy3 antibody. .. Lastly, the slides were stained with 0.25 μg/ml DAPI in 1× PBS solution, and then the images were observed and acquired under confocal microscopy (NOL-LSM 710; Carl Zeiss, Thornwood, NY) as described ( ).

    Article Title: The B chromosomes of the African cichlid fish Haplochromis obliquidens harbour 18S rRNA gene copies
    Article Snippet: .. Detection of hybridized probes was carried out with 0.07% avidin FITC conjugate (Sigma) in C buffer (0.1 M NaHCO3 /0.15 M NaCl) for 1 h, followed by two rounds of signal amplification using 2.5% anti-avidin biotin conjugate (Sigma) in blocking buffer (1.26% NaHCO3 , 0.018% sodium citrate, 0.0386% Triton X-100 an 1% non-fat dried milk) for 30 min. Each treatment with anti-avidin biotin conjugate was followed by a treatment with avidin-FITC. .. The treatments with avidin-FITC and anti-avidin-biotin were conducted in a 2× SSC moist chamber at 37°C.

    Avidin-Biotin Assay:

    Article Title: Reduction of food intake by cholecystokinin requires activation of hindbrain NMDA-type glutamate receptors
    Article Snippet: .. The avidin-biotin-antibody complex was revealed histochemically using a nickel-intensified diaminobenzidine reaction (Sigma). ..

    Article Title: Basic cytogenetics and physical mapping of 5S and 18S ribosomal genes in Hoplias malabaricus (Osteichthyes, Characiformes, Erythrinidae) from isolated natural lagoons: a conserved karyomorph along the Iguaçu river basin
    Article Snippet: .. The detection and amplification of the hybridization signal was performed using antidigoxigenin-rhodamine for 5S rDNA (Roche) and avidin-FITC and anti-avidin-biotin for 18S rDNA (Sigma). .. The best metaphases were captured in an Olympus BX60 photomicroscope with a digital camera DP71 and DPcontroller 3.2.1.276 software (Olympus).

    Article Title: The B chromosomes of the African cichlid fish Haplochromis obliquidens harbour 18S rRNA gene copies
    Article Snippet: .. Detection of hybridized probes was carried out with 0.07% avidin FITC conjugate (Sigma) in C buffer (0.1 M NaHCO3 /0.15 M NaCl) for 1 h, followed by two rounds of signal amplification using 2.5% anti-avidin biotin conjugate (Sigma) in blocking buffer (1.26% NaHCO3 , 0.018% sodium citrate, 0.0386% Triton X-100 an 1% non-fat dried milk) for 30 min. Each treatment with anti-avidin biotin conjugate was followed by a treatment with avidin-FITC. .. The treatments with avidin-FITC and anti-avidin-biotin were conducted in a 2× SSC moist chamber at 37°C.

    Blocking Assay:

    Article Title: The B chromosomes of the African cichlid fish Haplochromis obliquidens harbour 18S rRNA gene copies
    Article Snippet: .. Detection of hybridized probes was carried out with 0.07% avidin FITC conjugate (Sigma) in C buffer (0.1 M NaHCO3 /0.15 M NaCl) for 1 h, followed by two rounds of signal amplification using 2.5% anti-avidin biotin conjugate (Sigma) in blocking buffer (1.26% NaHCO3 , 0.018% sodium citrate, 0.0386% Triton X-100 an 1% non-fat dried milk) for 30 min. Each treatment with anti-avidin biotin conjugate was followed by a treatment with avidin-FITC. .. The treatments with avidin-FITC and anti-avidin-biotin were conducted in a 2× SSC moist chamber at 37°C.

    other:

    Article Title: Expression of green fluorescent protein defines a specific population of lamina II excitatory interneurons in the GRP::eGFP mouse
    Article Snippet: The secondary antibody mixture included fluorescent secondary antibodies to reveal eGFP, Homer and VGLUT3, together with a biotinylated antibody to reveal eGFP.

    Staining:

    Article Title: Extra Microchromosomes Play Male Determination Role in Polyploid Gibel Carp
    Article Snippet: .. The probe with Biotin was first stained with ExtraAvidin-Cy3 antibody (Sigma Chemical, St. Louis, MO) and the signal was amplified by orderly adding monoclonal Biotin-conjugated anti-avidin antibody (Sigma Chemical) and ExtraAvidin-Cy3 antibody. .. Lastly, the slides were stained with 0.25 μg/ml DAPI in 1× PBS solution, and then the images were observed and acquired under confocal microscopy (NOL-LSM 710; Carl Zeiss, Thornwood, NY) as described ( ).

    Hybridization:

    Article Title: Basic cytogenetics and physical mapping of 5S and 18S ribosomal genes in Hoplias malabaricus (Osteichthyes, Characiformes, Erythrinidae) from isolated natural lagoons: a conserved karyomorph along the Iguaçu river basin
    Article Snippet: .. The detection and amplification of the hybridization signal was performed using antidigoxigenin-rhodamine for 5S rDNA (Roche) and avidin-FITC and anti-avidin-biotin for 18S rDNA (Sigma). .. The best metaphases were captured in an Olympus BX60 photomicroscope with a digital camera DP71 and DPcontroller 3.2.1.276 software (Olympus).

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  • 99
    Millipore streptavidin linked hrp
    Conception and applicability of autotransporter (AT)—complement receptor ligand (CRL) fusions. ( a ) Graphical abstract. ( b ) The modification of YadA to yield YFC (YadA-FLAG-C3d) and YFP (YadA-FLAG-p28); SS: signal sequence. F: FLAG tag. ( c ) Heterologous expression of plasmid-borne YadA by Ec and Ft detected by western blot with α-YadA sera followed by horseradish peroxidase <t>(HRP)-conjugated</t> secondary Ab. Strains bearing empty vector are denoted by “-”. Arrows indicate the trimers and monomers of unmodified YadA. ( d ) Collagen-coated ELISA plates were incubated with serial dilutions of intact Ft :-, Ft :YadA, and Ft :YFC; bound bacteria were detected by α- Ft LPS Ab. ( e , f ) Whole cell lysates of Ec , Kp , and Ft containing empty vector (-) or the YFP or YFC expression vectors were probed by western blot with primary Ab specific for C3d and the FLAG epitope followed sequentially by biotinylated secondary Abs and <t>streptavidin-HRP</t> (SA-HRP). YFC and YFP trimers and monomers are designated with black and grey arrows. The ~20 kDa bands evident in all lanes are endogenously biotinylated bacterial proteins (annotated AccB in Ec and Ft [ 40 ]) detected by SA-HRP. ( g ) Intact bacteria as indicated were incubated in solution with α-FLAG Ab. ( h ) Intact Ft as indicated were incubated in solution with the indicated Ab. IglC is primarily a cytoplasmic Ft protein. Washed bacteria in ( g , h ) were probed for IgG heavy chain (HC), followed by biotinylated secondary Abs and SA-HRP.
    Streptavidin Linked Hrp, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin linked hrp/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptavidin linked hrp - by Bioz Stars, 2020-09
    99/100 stars
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    90
    Millipore avidin biotin immunoperoxidase technique
    Conception and applicability of autotransporter (AT)—complement receptor ligand (CRL) fusions. ( a ) Graphical abstract. ( b ) The modification of YadA to yield YFC (YadA-FLAG-C3d) and YFP (YadA-FLAG-p28); SS: signal sequence. F: FLAG tag. ( c ) Heterologous expression of plasmid-borne YadA by Ec and Ft detected by western blot with α-YadA sera followed by horseradish peroxidase <t>(HRP)-conjugated</t> secondary Ab. Strains bearing empty vector are denoted by “-”. Arrows indicate the trimers and monomers of unmodified YadA. ( d ) Collagen-coated ELISA plates were incubated with serial dilutions of intact Ft :-, Ft :YadA, and Ft :YFC; bound bacteria were detected by α- Ft LPS Ab. ( e , f ) Whole cell lysates of Ec , Kp , and Ft containing empty vector (-) or the YFP or YFC expression vectors were probed by western blot with primary Ab specific for C3d and the FLAG epitope followed sequentially by biotinylated secondary Abs and <t>streptavidin-HRP</t> (SA-HRP). YFC and YFP trimers and monomers are designated with black and grey arrows. The ~20 kDa bands evident in all lanes are endogenously biotinylated bacterial proteins (annotated AccB in Ec and Ft [ 40 ]) detected by SA-HRP. ( g ) Intact bacteria as indicated were incubated in solution with α-FLAG Ab. ( h ) Intact Ft as indicated were incubated in solution with the indicated Ab. IglC is primarily a cytoplasmic Ft protein. Washed bacteria in ( g , h ) were probed for IgG heavy chain (HC), followed by biotinylated secondary Abs and SA-HRP.
    Avidin Biotin Immunoperoxidase Technique, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin immunoperoxidase technique/product/Millipore
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    avidin biotin immunoperoxidase technique - by Bioz Stars, 2020-09
    90/100 stars
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    85
    Millipore biotinylated proteasome specific affinity probe adak
    Conception and applicability of autotransporter (AT)—complement receptor ligand (CRL) fusions. ( a ) Graphical abstract. ( b ) The modification of YadA to yield YFC (YadA-FLAG-C3d) and YFP (YadA-FLAG-p28); SS: signal sequence. F: FLAG tag. ( c ) Heterologous expression of plasmid-borne YadA by Ec and Ft detected by western blot with α-YadA sera followed by horseradish peroxidase <t>(HRP)-conjugated</t> secondary Ab. Strains bearing empty vector are denoted by “-”. Arrows indicate the trimers and monomers of unmodified YadA. ( d ) Collagen-coated ELISA plates were incubated with serial dilutions of intact Ft :-, Ft :YadA, and Ft :YFC; bound bacteria were detected by α- Ft LPS Ab. ( e , f ) Whole cell lysates of Ec , Kp , and Ft containing empty vector (-) or the YFP or YFC expression vectors were probed by western blot with primary Ab specific for C3d and the FLAG epitope followed sequentially by biotinylated secondary Abs and <t>streptavidin-HRP</t> (SA-HRP). YFC and YFP trimers and monomers are designated with black and grey arrows. The ~20 kDa bands evident in all lanes are endogenously biotinylated bacterial proteins (annotated AccB in Ec and Ft [ 40 ]) detected by SA-HRP. ( g ) Intact bacteria as indicated were incubated in solution with α-FLAG Ab. ( h ) Intact Ft as indicated were incubated in solution with the indicated Ab. IglC is primarily a cytoplasmic Ft protein. Washed bacteria in ( g , h ) were probed for IgG heavy chain (HC), followed by biotinylated secondary Abs and SA-HRP.
    Biotinylated Proteasome Specific Affinity Probe Adak, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated proteasome specific affinity probe adak/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated proteasome specific affinity probe adak - by Bioz Stars, 2020-09
    85/100 stars
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    Image Search Results


    Conception and applicability of autotransporter (AT)—complement receptor ligand (CRL) fusions. ( a ) Graphical abstract. ( b ) The modification of YadA to yield YFC (YadA-FLAG-C3d) and YFP (YadA-FLAG-p28); SS: signal sequence. F: FLAG tag. ( c ) Heterologous expression of plasmid-borne YadA by Ec and Ft detected by western blot with α-YadA sera followed by horseradish peroxidase (HRP)-conjugated secondary Ab. Strains bearing empty vector are denoted by “-”. Arrows indicate the trimers and monomers of unmodified YadA. ( d ) Collagen-coated ELISA plates were incubated with serial dilutions of intact Ft :-, Ft :YadA, and Ft :YFC; bound bacteria were detected by α- Ft LPS Ab. ( e , f ) Whole cell lysates of Ec , Kp , and Ft containing empty vector (-) or the YFP or YFC expression vectors were probed by western blot with primary Ab specific for C3d and the FLAG epitope followed sequentially by biotinylated secondary Abs and streptavidin-HRP (SA-HRP). YFC and YFP trimers and monomers are designated with black and grey arrows. The ~20 kDa bands evident in all lanes are endogenously biotinylated bacterial proteins (annotated AccB in Ec and Ft [ 40 ]) detected by SA-HRP. ( g ) Intact bacteria as indicated were incubated in solution with α-FLAG Ab. ( h ) Intact Ft as indicated were incubated in solution with the indicated Ab. IglC is primarily a cytoplasmic Ft protein. Washed bacteria in ( g , h ) were probed for IgG heavy chain (HC), followed by biotinylated secondary Abs and SA-HRP.

    Journal: Pathogens

    Article Title: Autotransporter-Mediated Display of Complement Receptor Ligands by Gram-Negative Bacteria Increases Antibody Responses and Limits Disease Severity

    doi: 10.3390/pathogens9050375

    Figure Lengend Snippet: Conception and applicability of autotransporter (AT)—complement receptor ligand (CRL) fusions. ( a ) Graphical abstract. ( b ) The modification of YadA to yield YFC (YadA-FLAG-C3d) and YFP (YadA-FLAG-p28); SS: signal sequence. F: FLAG tag. ( c ) Heterologous expression of plasmid-borne YadA by Ec and Ft detected by western blot with α-YadA sera followed by horseradish peroxidase (HRP)-conjugated secondary Ab. Strains bearing empty vector are denoted by “-”. Arrows indicate the trimers and monomers of unmodified YadA. ( d ) Collagen-coated ELISA plates were incubated with serial dilutions of intact Ft :-, Ft :YadA, and Ft :YFC; bound bacteria were detected by α- Ft LPS Ab. ( e , f ) Whole cell lysates of Ec , Kp , and Ft containing empty vector (-) or the YFP or YFC expression vectors were probed by western blot with primary Ab specific for C3d and the FLAG epitope followed sequentially by biotinylated secondary Abs and streptavidin-HRP (SA-HRP). YFC and YFP trimers and monomers are designated with black and grey arrows. The ~20 kDa bands evident in all lanes are endogenously biotinylated bacterial proteins (annotated AccB in Ec and Ft [ 40 ]) detected by SA-HRP. ( g ) Intact bacteria as indicated were incubated in solution with α-FLAG Ab. ( h ) Intact Ft as indicated were incubated in solution with the indicated Ab. IglC is primarily a cytoplasmic Ft protein. Washed bacteria in ( g , h ) were probed for IgG heavy chain (HC), followed by biotinylated secondary Abs and SA-HRP.

    Article Snippet: Secondary horseradish peroxidase (HRP)-conjugated antibodies were diluted to the indicated concentrations in PBST and incubated at 4 °C for 1 h. Where indicated, a biotinylated secondary antibody was used, followed by streptavidin-linked HRP, each for 1 h incubations at 4 °C.

    Techniques: Modification, Sequencing, FLAG-tag, Expressing, Plasmid Preparation, Western Blot, Enzyme-linked Immunosorbent Assay, Incubation